LOCUS       RC217003                9051 bp    DNA     circular  SYN 
DEFINITION  MET (Myc-DDK-tagged)-Human met proto-oncogene (hepatocyte growth factor receptor) (MET), transcript variant 2
ACCESSION   NM_000245
VERSION     NM_000245.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5208..5299 
                     /label="Myc-DDK"  
     misc_feature    5329..5914 
                     /label="PolyA signal"  
     misc_feature    5386..5405 
                     /label="Restriction Enzyme"  
     misc_feature    6190..6862 
                     /label="ColE1"  
     misc_feature    7292..8086 
                     /label="Kan/Neo"  
     misc_feature    8121..8423 
                     /label="SV40 ori"  
     misc_feature    8509..8986 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5190..5196 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5190 
                     /label="MET"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGGCCCCC GCTGTGCTTG CACCTGGCAT CCTCGTGCTC CTGTTTACCT 
     1081 TGGTGCAGAG GAGCAATGGG GAGTGTAAAG AGGCACTAGC AAAGTCCGAG ATGAATGTGA 
     1141 ATATGAAGTA TCAGCTTCCC AACTTCACCG CGGAAACACC CATCCAGAAT GTCATTCTAC 
     1201 ATGAGCATCA CATTTTCCTT GGTGCCACTA ACTACATTTA TGTTTTAAAT GAGGAAGACC 
     1261 TTCAGAAGGT TGCTGAGTAC AAGACTGGGC CTGTGCTGGA ACACCCAGAT TGTTTCCCAT 
     1321 GTCAGGACTG CAGCAGCAAA GCCAATTTAT CAGGAGGTGT TTGGAAAGAT AACATCAACA 
     1381 TGGCTCTAGT TGTCGACACC TACTATGATG ATCAACTCAT TAGCTGTGGC AGCGTCAACA 
     1441 GAGGGACCTG CCAGCGACAT GTCTTTCCCC ACAATCATAC TGCTGACATA CAGTCGGAGG 
     1501 TTCACTGCAT ATTCTCCCCA CAGATAGAAG AGCCCAGCCA GTGTCCTGAC TGTGTGGTGA 
     1561 GCGCCCTGGG AGCCAAAGTC CTTTCATCTG TAAAGGACCG GTTCATCAAC TTCTTTGTAG 
     1621 GCAATACCAT AAATTCTTCT TATTTCCCAG ATCATCCATT GCATTCGATA TCAGTGAGAA 
     1681 GGCTAAAGGA AACGAAAGAT GGTTTTATGT TTTTGACGGA CCAGTCCTAC ATTGATGTTT 
     1741 TACCTGAGTT CAGAGATTCT TACCCCATTA AGTATGTCCA TGCCTTTGAA AGCAACAATT 
     1801 TTATTTACTT CTTGACGGTC CAAAGGGAAA CTCTAGATGC TCAGACTTTT CACACAAGAA 
     1861 TAATCAGGTT CTGTTCCATA AACTCTGGAT TGCATTCCTA CATGGAAATG CCTCTGGAGT 
     1921 GTATTCTCAC AGAAAAGAGA AAAAAGAGAT CCACAAAGAA GGAAGTGTTT AATATACTTC 
     1981 AGGCTGCGTA TGTCAGCAAG CCTGGGGCCC AGCTTGCTAG ACAAATAGGA GCCAGCCTGA 
     2041 ATGATGACAT TCTTTTCGGG GTGTTCGCAC AAAGCAAGCC AGATTCTGCC GAACCAATGG 
     2101 ATCGATCTGC CATGTGTGCA TTCCCTATCA AATATGTCAA CGACTTCTTC AACAAGATCG 
     2161 TCAACAAAAA CAATGTGAGA TGTCTCCAGC ATTTTTACGG ACCCAATCAT GAGCACTGCT 
     2221 TTAATAGGAC ACTTCTGAGA AATTCATCAG GCTGTGAAGC GCGCCGTGAT GAATATCGAA 
     2281 CAGAGTTTAC CACAGCTTTG CAGCGCGTTG ACTTATTCAT GGGTCAATTC AGCGAAGTCC 
     2341 TCTTAACATC TATATCCACC TTCATTAAAG GAGACCTCAC CATAGCTAAT CTTGGGACAT 
     2401 CAGAGGGTCG CTTCATGCAG GTTGTGGTTT CTCGATCAGG ACCATCAACC CCTCATGTGA 
     2461 ATTTTCTCCT GGACTCCCAT CCAGTGTCTC CAGAAGTGAT TGTGGAGCAT ACATTAAACC 
     2521 AAAATGGCTA CACACTGGTT ATCACTGGGA AGAAGATCAC GAAGATCCCA TTGAATGGCT 
     2581 TGGGCTGCAG ACATTTCCAG TCCTGCAGTC AATGCCTCTC TGCCCCACCC TTTGTTCAGT 
     2641 GTGGCTGGTG CCACGACAAA TGTGTGCGAT CGGAGGAATG CCTGAGCGGG ACATGGACTC 
     2701 AACAGATCTG TCTGCCTGCA ATCTACAAGG TTTTCCCAAA TAGTGCACCC CTTGAAGGAG 
     2761 GGACAAGGCT GACCATATGT GGCTGGGACT TTGGATTTCG GAGGAATAAT AAATTTGATT 
     2821 TAAAGAAAAC TAGAGTTCTC CTTGGAAATG AGAGCTGCAC CTTGACTTTA AGTGAGAGCA 
     2881 CGATGAATAC ATTGAAATGC ACAGTTGGTC CTGCCATGAA TAAGCATTTC AATATGTCCA 
     2941 TAATTATTTC AAATGGCCAC GGGACAACAC AATACAGTAC ATTCTCCTAT GTGGATCCTG 
     3001 TAATAACAAG TATTTCGCCG AAATACGGTC CTATGGCTGG TGGCACTTTA CTTACTTTAA 
     3061 CTGGAAATTA CCTAAACAGT GGGAATTCTA GACACATTTC AATTGGTGGA AAAACATGTA 
     3121 CTTTAAAAAG TGTGTCAAAC AGTATTCTTG AATGTTATAC CCCAGCCCAA ACCATTTCAA 
     3181 CTGAGTTTGC TGTTAAATTG AAAATTGACT TAGCCAACCG AGAGACAAGC ATCTTCAGTT 
     3241 ACCGTGAAGA TCCCATTGTC TATGAAATTC ATCCAACCAA ATCTTTTATT AGTGGTGGGA 
     3301 GCACAATAAC AGGTGTTGGG AAAAACCTGA ATTCAGTTAG TGTCCCGAGA ATGGTCATAA 
     3361 ATGTGCATGA AGCAGGAAGG AACTTTACAG TGGCATGTCA ACATCGCTCT AATTCAGAGA 
     3421 TAATCTGTTG TACCACTCCT TCCCTGCAAC AGCTGAATCT GCAACTCCCC CTGAAAACCA 
     3481 AAGCCTTTTT CATGTTAGAT GGGATCCTTT CCAAATACTT TGATCTCATT TATGTACATA 
     3541 ATCCTGTGTT TAAGCCTTTT GAAAAGCCAG TGATGATCTC AATGGGCAAT GAAAATGTAC 
     3601 TGGAAATTAA GGGAAATGAT ATTGACCCTG AAGCAGTTAA AGGTGAAGTG TTAAAAGTTG 
     3661 GAAATAAGAG CTGTGAGAAT ATACACTTAC ATTCTGAAGC CGTTTTATGC ACGGTCCCCA 
     3721 ATGACCTGCT GAAATTGAAC AGCGAGCTAA ATATAGAGTG GAAGCAAGCA ATTTCTTCAA 
     3781 CCGTCCTTGG AAAAGTAATA GTTCAACCAG ATCAGAATTT CACAGGATTG ATTGCTGGTG 
     3841 TTGTCTCAAT ATCAACAGCA CTGTTATTAC TACTTGGGTT TTTCCTGTGG CTGAAAAAGA 
     3901 GAAAGCAAAT TAAAGATCTG GGCAGTGAAT TAGTTCGCTA CGATGCAAGA GTACACACTC 
     3961 CTCATTTGGA TAGGCTTGTA AGTGCCCGAA GTGTAAGCCC AACTACAGAA ATGGTTTCAA 
     4021 ATGAATCTGT AGACTACCGA GCTACTTTTC CAGAAGATCA GTTTCCTAAT TCATCTCAGA 
     4081 ACGGTTCATG CCGACAAGTG CAGTATCCTC TGACAGACAT GTCCCCCATC CTAACTAGTG 
     4141 GGGACTCTGA TATATCCAGT CCATTACTGC AAAATACTGT CCACATTGAC CTCAGTGCTC 
     4201 TAAATCCAGA GCTGGTCCAG GCAGTGCAGC ATGTAGTGAT TGGGCCCAGT AGCCTGATTG 
     4261 TGCATTTCAA TGAAGTCATA GGAAGAGGGC ATTTTGGTTG TGTATATCAT GGGACTTTGT 
     4321 TGGACAATGA TGGCAAGAAA ATTCACTGTG CTGTGAAATC CTTGAACAGA ATCACTGACA 
     4381 TAGGAGAAGT TTCCCAATTT CTGACCGAGG GAATCATCAT GAAAGATTTT AGTCATCCCA 
     4441 ATGTCCTCTC GCTCCTGGGA ATCTGCCTGC GAAGTGAAGG GTCTCCGCTG GTGGTCCTAC 
     4501 CATACATGAA ACATGGAGAT CTTCGAAATT TCATTCGAAA TGAGACTCAT AATCCAACTG 
     4561 TAAAAGATCT TATTGGCTTT GGTCTTCAAG TAGCCAAAGG CATGAAATAT CTTGCAAGCA 
     4621 AAAAGTTTGT CCACAGAGAC TTGGCTGCAA GAAACTGTAT GCTGGATGAA AAATTCACAG 
     4681 TCAAGGTTGC TGATTTTGGT CTTGCCAGAG ACATGTATGA TAAAGAATAC TATAGTGTAC 
     4741 ACAACAAAAC AGGTGCAAAG CTGCCAGTGA AGTGGATGGC TTTGGAAAGT CTGCAAACTC 
     4801 AAAAGTTTAC CACCAAGTCA GATGTGTGGT CCTTTGGCGT GCTCCTCTGG GAGCTGATGA 
     4861 CAAGAGGAGC CCCACCTTAT CCTGATGTAA ACACCTTTGA TATAACTGTT TACTTGTTGC 
     4921 AAGGGAGAAG ACTCCTACAA CCCGAATACT GCCCAGACCC CTTATATGAA GTAATGCTAA 
     4981 AATGCTGGCA CCCTAAAGCC GAAATGCGCC CATCCTTTTC TGAACTGGTG TCCCGGATAT 
     5041 CAGCAATCTT CTCTACTTTC ATTGGGGAGC ACTATGTCCA TGTGAACGCT ACTTATGTGA 
     5101 ACGTAAAATG TGTCGCTCCA TATCCTTCTC TGTTGTCATC AGAAGATAAC GCTGATGATG 
     5161 AGGTGGACAC ACGACCAGCC TCCTTCTGGG AGACATCAAC GCGTACGCGG CCGCTCGAGC 
     5221 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     5281 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     5341 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     5401 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     5461 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     5521 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     5581 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     5641 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     5701 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     5761 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     5821 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     5881 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     5941 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     6001 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     6061 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     6121 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     6181 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     6241 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     6301 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     6361 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     6421 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     6481 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     6541 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     6601 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     6661 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     6721 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     6781 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     6841 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     6901 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     6961 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     7021 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     7081 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     7141 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     7201 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     7261 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     7321 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     7381 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     7441 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     7501 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     7561 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     7621 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     7681 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     7741 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     7801 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     7861 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     7921 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     7981 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     8041 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     8101 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     8161 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     8221 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     8281 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     8341 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     8401 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     8461 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     8521 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     8581 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     8641 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     8701 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     8761 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     8821 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     8881 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     8941 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     9001 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
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