LOCUS       RC216972L2                9213 bp    DNA     circular  SYN 
DEFINITION  PKP1 (Myc-DDK-tagged)-Human plakophilin 1 (ectodermal dysplasia/skin fragility syndrome) (PKP1), transcript variant 1a
ACCESSION   NM_001005337
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7504..7684 
                     /label="3-LTR"  
     misc_feature    833..1013 
                     /label="5-LTR"  
     misc_feature    8412..9071 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV"  
     misc_feature    7732..8351 
                     /label="ColE1"  
     misc_feature    6379..6399 
                     /label="LR50"  
     misc_feature    5519..6244 
                     /label="mGFP"  
     misc_feature    3252..3271 
                     /label="T7"  
     misc_feature    6388..6979 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5498..5504 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5498 
                     /label="PKP1"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GAACCACTCG CCGCTCAAGA CCGCCTTGGC 
     3361 GTACGAATGC TTCCAGGACC AGGACAACTC CACGTTGGCT TTGCCGTCGG ACCAAAAGAT 
     3421 GAAAACAGGC ACGTCTGGCA GGCAGCGCGT GCAGGAGCAG GTGATGATGA CCGTCAAGCG 
     3481 GCAGAAGTCC AAGTCTTCCC AGTCGTCCAC CCTGAGCCAC TCCAATCGAG GTTCCATGTA 
     3541 TGATGGCTTG GCTGACAATT ACAACTATGG GACCACCAGC AGGAGCAGCT ACTACTCCAA 
     3601 GTTCCAGGCA GGGAATGGCT CATGGGGATA TCCGATCTAC AATGGAACCC TCAAGCGGGA 
     3661 GCCTGACAAC AGGCGCTTCA GCTCCTACAG CCAGATGGAG AACTGGAGCC GGCACTACCC 
     3721 CCGGGGCAGC TGTAACACCA CCGGCGCAGG CAGCGACATC TGCTTCATGC AGAAAATCAA 
     3781 GGCGAGCCGC AGTGAGCCCG ACCTCTACTG TGACCCACGG GGCACCCTGC GCAAGGGCAC 
     3841 GCTGGGCAGC AAGGGCCAGA AGACCACCCA GAACCGCTAC AGCTTTTACA GCACCTGCAG 
     3901 TGGTCAGAAG GCCATAAAGA AGTGCCCTGT GCGCCCGCCC TCTTGTGCCT CCAAGCAGGA 
     3961 CCCTGTGTAT ATCCCGCCCA TCTCCTGCAA CAAGGACCTG TCCTTTGGCC ACTCTAGGGC 
     4021 CAGCTCCAAG ATCTGCAGTG AGGACATCGA GTGCAGTGGG CTGACCATCC CCAAGGCTGT 
     4081 GCAGTACCTG AGCTCCCAGG ATGAGAAGTA CCAGGCCATT GGGGCCTATT ACATCCAGCA 
     4141 TACCTGCTTC CAGGATGAAT CTGCCAAGCA ACAGGTCTAT CAGCTGGGAG GCATCTGCAA 
     4201 GCTGGTGGAC CTCCTCCGCA GCCCCAACCA GAACGTCCAG CAGGCTGCGG CAGGGGCCCT 
     4261 GCGCAACCTG GTGTTCAGGA GCACCACCAA CAAGCTGGAG ACCCGGAGGC AGAATGGGAT 
     4321 CCGCGAGGCA GTCAGCCTCC TGAGGAGAAC CGGGAACGCC GAGATCCAGA AGCAGCTGAC 
     4381 TGGGCTGCTC TGGAACCTGT CTTCCACTGA CGAGCTGAAG GAGGAACTCA TTGCCGACGC 
     4441 CCTGCCTGTT CTGGCCGACC GCGTCATCAT TCCCTTCTCT GGCTGGTGCG ATGGCAATAG 
     4501 CAACATGTCC CGGGAAGTGG TGGACCCTGA GGTCTTCTTC AATGCCACAG GCTGCTTGAG 
     4561 GAACCTGAGC TCGGCCGATG CAGGCCGCCA GACCATGCGT AACTACTCAG GGCTCATTGA 
     4621 TTCCCTCATG GCCTATGTCC AGAACTGTGT AGCGGCCAGC CGCTGTGACG ACAAGTCTGT 
     4681 GGAAAACTGC ATGTGTGTTC TGCACAACCT CTCCTACCGC CTGGACGCCG AGGTGCCCAC 
     4741 CCGCTACCGC CAGCTGGAGT ATAACGCCCG CAACGCCTAC ACCGAGAAGT CCTCCACTGG 
     4801 CTGCTTCAGC AACAAGAGCG ACAAGATGAT GAACAACAAC TATGACTGCC CCCTGCCTGA 
     4861 GGAAGAGACC AACCCCAAGG GCAGCGGCTG GTTGTACCAT TCAGATGCCA TCCGCACCTA 
     4921 CCTGAACCTC ATGGGCAAGA GCAAGAAAGA TGCTACCCTG GAGGCCTGTG CTGGTGCCCT 
     4981 GCAGAACCTG ACAGCCAGCA AGGGGCTGAT GTCCAGTGGC ATGAGCCAGT TGATTGGGCT 
     5041 GAAGGAAAAG GGCCTGCCAC AAATTGCCCG CCTCCTGCAA TCTGGCAACT CTGATGTGGT 
     5101 GCGGTCCGGA GCCTCCCTCC TGAGCAACAT GTCCCGCCAC CCTCTGCTGC ACAGAGTGAT 
     5161 GGGGAACCAG GTGTTCCCGG AGGTGACCAG GCTCCTCACC AGCCACACTG GCAATACCAG 
     5221 CAACTCCGAA GACATCTTGT CCTCGGCCTG CTACACTGTG AGGAACCTGA TGGCCTCGCA 
     5281 GCCACAACTG GCCAAGCAGT ACTTCTCCAG CAGCATGCTC AACAACATCA TCAACCTGTG 
     5341 CCGAAGCAGT GCCTCACCCA AGGCCGCAGA AGCTGCCCGG CTTCTCCTGT CTGACATGTG 
     5401 GTCCAGCAAG GAACTGCAGG GTGTCCTCAG ACAGCAAGGT TTCGATAGGA ACATGCTGGG 
     5461 AACCTTAGCT GGGGCCAACA GCCTCAGGAA CTTCACCTCC CGATTCACGC GTACGCGGCC 
     5521 GCTCGAGATG AGCGGGGGCG AGGAGCTGTT CGCCGGCATC GTGCCCGTGC TGATCGAGCT 
     5581 GGACGGCGAC GTGCACGGCC ACAAGTTCAG CGTGCGCGGC GAGGGCGAGG GCGACGCCGA 
     5641 CTACGGCAAG CTGGAGATCA AGTTCATCTG CACCACCGGC AAGCTGCCCG TGCCCTGGCC 
     5701 CACCCTGGTG ACCACCCTCT GCTACGGCAT CCAGTGCTTC GCCCGCTACC CCGAGCACAT 
     5761 GAAGATGAAC GACTTCTTCA AGAGCGCCAT GCCCGAGGGC TACATCCAGG AGCGCACCAT 
     5821 CCAGTTCCAG GACGACGGCA AGTACAAGAC CCGCGGCGAG GTGAAGTTCG AGGGCGACAC 
     5881 CCTGGTGAAC CGCATCGAGC TGAAGGGCAA GGACTTCAAG GAGGACGGCA ACATCCTGGG 
     5941 CCACAAGCTG GAGTACAGCT TCAACAGCCA CAACGTGTAC ATCCGCCCCG ACAAGGCCAA 
     6001 CAACGGCCTG GAGGCTAACT TCAAGACCCG CCACAACATC GAGGGCGGCG GCGTGCAGCT 
     6061 GGCCGACCAC TACCAGACCA ACGTGCCCCT GGGCGACGGC CCCGTGCTGA TCCCCATCAA 
     6121 CCACTACCTG AGCACTCAGA CCAAGATCAG CAAGGACCGC AACGAGGCCC GCGACCACAT 
     6181 GGTGCTCCTG GAGTCCTTCA GCGCCTGCTG CCACACCCAC GGCATGGACG AGCTGTACAG 
     6241 GTCCGGACTC AGATAAGTTT AAACGGCCGG CCGCGGTCTG TACAAGTAGG ATTCGTCGAG 
     6301 GGACCTAATA ACTTCGTATA GCATACATTA TACGAAGTTA TACATGTTTA AGGGTTCCGG 
     6361 TTCCACTAGG TACAATTCGA TATCAAGCTT ATCGATAATC AACCTCTGGA TTACAAAATT 
     6421 TGTGAAAGAT TGACTGGTAT TCTTAACTAT GTTGCTCCTT TTACGCTATG TGGATACGCT 
     6481 GCTTTAATGC CTTTGTATCA TGCTATTGCT TCCCGTATGG CTTTCATTTT CTCCTCCTTG 
     6541 TATAAATCCT GGTTGCTGTC TCTTTATGAG GAGTTGTGGC CCGTTGTCAG GCAACGTGGC 
     6601 GTGGTGTGCA CTGTGTTTGC TGACGCAACC CCCACTGGTT GGGGCATTGC CACCACCTGT 
     6661 CAGCTCCTTT CCGGGACTTT CGCTTTCCCC CTCCCTATTG CCACGGCGGA ACTCATCGCC 
     6721 GCCTGCCTTG CCCGCTGCTG GACAGGGGCT CGGCTGTTGG GCACTGACAA TTCCGTGGTG 
     6781 TTGTCGGGGA AATCATCGTC CTTTCCTTGG CTGCTCGCCT GTGTTGCCAC CTGGATTCTG 
     6841 CGCGGGACGT CCTTCTGCTA CGTCCCTTCG GCCCTCAATC CAGCGGACCT TCCTTCCCGC 
     6901 GGCCTGCTGC CGGCTCTGCG GCCTCTTCCG CGTCTTCGCC TTCGCCCTCA GACGAGTCGG 
     6961 ATCTCCCTTT GGGCCGCCTC CCCGCATCGA TACCGTCGAC CTCGATCGAG ACCTAGAAAA 
     7021 ACATGGAGCA ATCACAAGTA GCAATACAGC AGCTACCAAT GCTGATTGTG CCTGGCTAGA 
     7081 AGCACAAGAG GAGGAGGAGG TGGGTTTTCC AGTCACACCT CAGGTACCTT TAAGACCAAT 
     7141 GACTTACAAG GCAGCTGTAG ATCTTAGCCA CTTTTTAAAA GAAAAGGGGG GACTGGAAGG 
     7201 GCTAATTCAC TCCCAACGAA GACAAGATAT CCTTGATCTG TGGATCTACC ACACACAAGG 
     7261 CTACTTCCCT GATTGGCAGA ACTACACACC AGGGCCAGGG ATCAGATATC CACTGACCTT 
     7321 TGGATGGTGC TACAAGCTAG TACCAGTTGA GCAAGAGAAG GTAGAAGAAG CCAATGAAGG 
     7381 AGAGAACACC CGCTTGTTAC ACCCTGTGAG CCTGCATGGG ATGGATGACC CGGAGAGAGA 
     7441 AGTATTAGAG TGGAGGTTTG ACAGCCGCCT AGCATTTCAT CACATGGCCC GAGAGCTGCA 
     7501 TCCGGACTGT ACTGGGTCTC TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA 
     7561 ACTAGGGAAC CCACTGCTTA AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG 
     7621 TGCCCGTCTG TTGTGTGACT CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG 
     7681 GAAAATCTCT AGCAGCATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     7741 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     7801 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     7861 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     7921 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     7981 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     8041 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     8101 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     8161 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     8221 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     8281 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     8341 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     8401 TTAAGGGATT TTGGTCATGA TTACGCCCCG CCCTGCCACT CATCGCAGTA CTGTTGTAAT 
     8461 TCATTAAGCA TTCTGCCGAC ATGGAAGCCA TCACAAACGG CATGATGAAC CTGAATCGCC 
     8521 AGCGGCATCA GCACCTTGTC GCCTTGCGTA TAATATTTGC CCATGGTGAA AACGGGGGCG 
     8581 AAGAAGTTGT CCATATTGGC CACGTTTAAA TCAAAACTGG TGAAACTCAC CCAGGGATTG 
     8641 GCTGAGACGA AAAACATATT CTCAATAAAC CCTTTAGGGA AATAGGCCAG GTTTTCACCG 
     8701 TAACACGCCA CATCTTGCGA ATATATGTGT AGAAACTGCC GGAAATCGTC GTGGTATTCA 
     8761 CTCCAGAGCG ATGAAAACGT TTCAGTTTGC TCATGGAAAA CGGTGTAACA AGGGTGAACA 
     8821 CTATCCCATA TCACCAGCTC ACCGTCTTTC ATTGCCATAC GGAACTCCGG ATGAGCATTC 
     8881 ATCAGGCGGG CAAGAATGTG AATAAAGGCC GGATAAAACT TGTGCTTATT TTTCTTTACG 
     8941 GTCTTTAAAA AGGCCGTAAT ATCCAGCTGA ACGGTCTGGT TATAGGTACA TTGAGCAACT 
     9001 GACTGAAATG CCTCAAAATG TTCTTTACGA TGCCATTGGG ATATATCAAC GGTGGTATAT 
     9061 CCAGTGATTT TTTTCTCCAT ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     9121 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     9181 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GAC
//