LOCUS       RC216923                8175 bp    DNA     circular  SYN 
DEFINITION  AASDH (Myc-DDK-tagged)-Human aminoadipate-semialdehyde dehydrogenase (AASDH)
ACCESSION   NM_181806
VERSION     NM_181806.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4332..4423 
                     /label="Myc-DDK"  
     misc_feature    4453..5038 
                     /label="PolyA signal"  
     misc_feature    4510..4529 
                     /label="Restriction Enzyme"  
     misc_feature    5314..5986 
                     /label="ColE1"  
     misc_feature    6416..7210 
                     /label="Kan/Neo"  
     misc_feature    7245..7547 
                     /label="SV40 ori"  
     misc_feature    7633..8110 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4314..4320 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4314 
                     /label="AASDH"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACTCTTCAG GAATTGGTGC ATAAGGCTGC CTCCTGTTAT ATGGACAGAG 
     1081 TAGCTGTATG TTTTGATGAA TGCAACAACC AGCTTCCAGT TTACTACACC TACAAGACTG 
     1141 TGGTTAATGC TGCTTCTGAA TTATCAAATT TTCTGCTGTT ACACTGTGAC TTTCAAGGAA 
     1201 TTCGGGAAAT TGGTCTCTAC TGCCAACCTG GGATAGACTT ACCCTCTTGG ATTTTAGGAA 
     1261 TTCTCCAAGT CCCGGCTGCT TATGTACCTA TCGAGCCAGA TTCACCACCG TCATTATCAA 
     1321 CTCATTTTAT GAAAAAATGT AATCTAAAGT ATATCCTTGT TGAAAAAAAA CAAATTAATA 
     1381 AATTTAAATC TTTTCATGAA ACATTATTGA ACTATGATAC ATTTACAGTG GAACATAATG 
     1441 ACCTAGTGCT CTTCAGACTT CACTGGAAAA ATACTGAGGT GAACTTGATG CTAAATGATG 
     1501 GAAAAGAGAA ATATGAAAAA GAAAAAATAA AAAGCATAAG TTCTGAGCAT GTCAATGAAG 
     1561 AAAAAGCAGA AGAACACATG GATCTGAGGC TAAAGCATTG CTTAGCCTAT GTTCTACATA 
     1621 CATCAGGGAC TACAGGGATA CCGAAGATTG TCAGAGTGCC TCATAAGTGT ATAGTACCAA 
     1681 ATATCCAGCA TTTTCGGGTA CTTTTTGACA TCACACAAGA AGATGTTTTG TTTCTGGCTT 
     1741 CACCTCTGAC CTTCGATCCT TCTGTTGTGG AAATATTTCT TGCTCTATCA AGTGGTGCCT 
     1801 CTCTGCTTAT TGTACCAACT TCCGTCAAGT TGCTCCCATC AAAATTAGCC AGCGTTCTCT 
     1861 TTTCCCATCA TAGAGTGACT GTTTTGCAGG CAACACCAAC ATTGCTTAGA AGATTTGGAT 
     1921 CTCAGCTTAT CAAGTCAACT GTTTTGTCAG CCACTACTTC TCTTCGAGTA TTAGCCCTTG 
     1981 GTGGTGAAGC GTTTCCATCA TTGACAGTTC TCAGAAGCTG GAGAGGAGAA GGCAATAAAA 
     2041 CACAAATATT TAATGTTTAT GGTATCACAG AGGTATCAAG TTGGGCGACC ATTTATAGGA 
     2101 TTCCAGAGAA GACTCTTAAC TCTACTCTCA AATGTGAATT GCCTGTACAA CTGGGATTTC 
     2161 CACTTCTTGG AACAGTAGTT GAAGTCAGAG ATACTAATGG CTTCACAATT CAGGAAGGCA 
     2221 GTGGCCAAGT ATTTTTAGGT GGCAGAAACA GAGTGTGTTT TCTTGATGAT GAAGTGACAG 
     2281 TACCACTTGG CACAATGCGA GCTACAGGAG ACTTTGTGAC TGTGAAAGAT GGAGAGATTT 
     2341 TTTTTTTGGG ACGAAAAGAC AGTCAGATCA AACGTCATGG CAAACGTCTT AACATTGAAC 
     2401 TTGTGCAACA GGTTGCTGAA GAGCTTCAGC AAGTGGAGTC TTGTGCAGTT ACATGGTATA 
     2461 ATCAGGAAAA ATTAATTCTC TTCATGGTGT CTAAAGATGC TTCAGTAAAA GAATACATCT 
     2521 TTAAAGAACT GCAGAAATAT CTTCCAAGTC ATGCAGTCCC GGATGAGCTT GTATTGATCG 
     2581 ACTCTCTACC ATTTACATCC CACGGCAAAA TTGATGTTTC TGAGTTAAAC AAGATATATT 
     2641 TAAACTACAT AAACTTGAAG TCTGAGAATA AGCTCAGTGG GAAAGAGGAC CTTTGGGAAA 
     2701 AATTACAGTA TTTGTGGAAG TCTACTCTGA ATCTCCCAGA AGATCTTTTG AGGGTTCCTG 
     2761 ATGAGTCACT CTTCTTAAAT AGTGGTGGAG ATTCCTTAAA GTCCATCCGG CTCCTCAGTG 
     2821 AGATTGAAAA ACTTGTTGGT ACATCAGTAC CTGGGCTTCT GGAAATTATT CTCAGCAGTT 
     2881 CCATTTTAGA GATTTATAAT CACATCCTTC AAACAGTGGT TCCAGATGAA GATGTGACAT 
     2941 TCAGGAAGAG TTGTGCCACA AAAAGGAAAC TCAGCGACAT TAATCAAGAG GAAGCCAGTG 
     3001 GAACATCTTT ACATCAGAAA GCCATCATGA CTTTCACTTG CCACAATGAG ATTAATGCTT 
     3061 TTGTTGTACT GAGCAGAGGG AGTCAAATTT TGTCTCTGAA TTCCACTAGG TTTTTAACAA 
     3121 AGTTAGGACA TTGCTCTTCA GCCTGTCCTT CTGACTCAGT TTCACAGACC AACATTCAAA 
     3181 ATTTGAAAGG CTTAAATTCT CCAGTTCTTA TTGGGAAGTC AAAAGATCCA TCCTGTGTTG 
     3241 CAAAAGTTTC TGAAGAGGGG AAACCTGCGA TAGGGACTCA GAAAATGGAG TTACATGTGA 
     3301 GGTGGAGGTC AGACACAGGC AAATGTGTAG ATGCTTCACC GCTGGTTGTA ATACCCACTT 
     3361 TTGATAAGTC ATCTACAACT GTGTACATTG GTTCCCATTC TCATAGAATG AAGGCAGTTG 
     3421 ACTTTTACTC TGGGAAGGTA AAATGGGAAC AGATTTTGGG AGATCGAATT GAATCCTCAG 
     3481 CATGTGTATC TAAGTGTGGA AACTTTATTG TGGTGGGCTG TTATAATGGA TTAGTTTATG 
     3541 TTCTGAAAAG TAATAGTGGA GAAAAATACT GGATGTTTAC TACTGAAGAT GCTGTCAAAA 
     3601 GCTCGGCAAC CATGGATCCA ACCACAGGAC TCATTTACAT TGGATCTCAT GACCAGCACG 
     3661 CATATGCTTT AGATATTTAT AGAAAGAAGT GTGTTTGGAA GTCAAAATGT GGAGGAACTG 
     3721 TCTTTTCCTC TCCGTGTTTG AACCTGATTC CACATCATTT GTATTTTGCT ACATTGGGAG 
     3781 GACTTTTACT GGCTGTAAAT CCTGCTACTG GGAACGTTAT TTGGAAACAT TCCTGTGGAA 
     3841 AACCACTCTT CTCTTCCCCA CAATGTTGCT CACAGTATAT TTGTATTGGC TGTGTAGATG 
     3901 GGAATTTACT CTGCTTTACT CACTTTGGAG AACAGGTTTG GCAGTTCTCT ACCAGTGGAC 
     3961 CAATCTTTTC ATCCCCGTGT ACCTCACCAT CAGAGCAAAA AATATTTTTT GGTTCCCATG 
     4021 ATTGCTTTAT CTACTGTTGT AACATGAAAG GTCACCTGCA GTGGAAATTT GAAACTACTT 
     4081 CAAGGGTCTA TGCAACACCG TTTGCTTTCC ATAACTACAA TGGCAGCAAT GAAATGTTGC 
     4141 TGGCAGCAGC ATCTACTGAT GGGAAAGTGT GGATCTTGGA ATCTCAGAGT GGACAATTGC 
     4201 AAAGTGTTTA TGAACTTCCT GGAGAAGTCT TCTCTTCTCC TGTGGTCCTG GAATCAATGC 
     4261 TCATTATTGG GTGTAGAGAT AATTATGTTT ATTGTCTGGA TTTATTGGGT GGCAATCAAA 
     4321 AAACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     4381 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4441 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4501 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4561 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4621 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4681 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     4741 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     4801 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     4861 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     4921 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     4981 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     5041 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     5101 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     5161 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     5221 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     5281 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     5341 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     5401 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     5461 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     5521 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     5581 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     5641 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     5701 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     5761 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     5821 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     5881 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     5941 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     6001 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     6061 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     6121 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     6181 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     6241 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     6301 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     6361 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     6421 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     6481 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     6541 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     6601 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     6661 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     6721 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     6781 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     6841 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     6901 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     6961 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     7021 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     7081 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     7141 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     7201 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     7261 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     7321 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     7381 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     7441 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     7501 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7561 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     7621 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     7681 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     7741 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     7801 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     7861 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     7921 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     7981 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     8041 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     8101 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     8161 TTTAACGCGA ATTTT
//