LOCUS       RC216892                8421 bp    DNA     circular  SYN 
DEFINITION  GRM5 (Myc-DDK-tagged)-Human glutamate receptor, metabotropic 5 (GRM5), transcript variant b
ACCESSION   NM_000842
VERSION     NM_000842.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4578..4669 
                     /label="Myc-DDK"  
     misc_feature    4699..5284 
                     /label="PolyA signal"  
     misc_feature    4756..4775 
                     /label="Restriction Enzyme"  
     misc_feature    5560..6232 
                     /label="ColE1"  
     misc_feature    6662..7456 
                     /label="Kan/Neo"  
     misc_feature    7491..7793 
                     /label="SV40 ori"  
     misc_feature    7879..8356 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4560..4566 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4560 
                     /label="GRM5"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTCCTTCTG TTGATCCTGT CAGTCTTACT TTTGAAAGAA GATGTCCGTG 
     1081 GGAGTGCACA GTCCAGTGAG AGGAGGGTGG TGGCTCACAT GCCGGGTGAC ATCATTATTG 
     1141 GAGCTCTCTT TTCTGTTCAT CACCAGCCTA CTGTGGACAA AGTTCATGAG AGGAAGTGTG 
     1201 GGGCGGTCCG TGAACAGTAT GGCATTCAGA GAGTGGAGGC CATGCTGCAT ACCCTGGAAA 
     1261 GGATCAATTC AGACCCCACA CTCTTGCCCA ACATCACACT GGGCTGTGAG ATAAGGGACT 
     1321 CCTGCTGGCA TTCGGCTGTG GCCCTAGAGC AGAGCATTGA GTTCATAAGA GATTCCCTCA 
     1381 TTTCTTCAGA AGAGGAAGAA GGCTTGGTAC GCTGTGTGGA TGGCTCCTCC TCTTCCTTCC 
     1441 GCTCCAAGAA GCCCATAGTA GGGGTCATTG GGCCTGGCTC CAGTTCTGTA GCCATTCAGG 
     1501 TCCAGAATTT GCTCCAGCTT TTCAACATAC CTCAGATTGC TTACTCAGCA ACCAGCATGG 
     1561 ATCTGAGTGA CAAGACTCTG TTCAAATATT TCATGAGGGT TGTGCCTTCA GATGCTCAGC 
     1621 AGGCAAGGGC CATGGTGGAC ATAGTGAAGA GGTACAACTG GACCTATGTA TCAGCCGTGC 
     1681 ACACAGAAGG CAACTATGGA GAAAGTGGGA TGGAAGCCTT CAAAGATATG TCAGCGAAGG 
     1741 AAGGGATTTG CATCGCCCAC TCTTACAAAA TCTACAGTAA TGCAGGGGAG CAGAGCTTTG 
     1801 ATAAGCTGCT GAAGAAGCTC ACAAGTCACT TGCCCAAGGC CCGGGTGGTG GCCTGCTTCT 
     1861 GTGAGGGCAT GACGGTGAGA GGTCTGCTGA TGGCCATGAG GCGCCTGGGT CTAGCGGGAG 
     1921 AATTTCTGCT TCTGGGCAGT GATGGCTGGG CTGACAGGTA TGATGTGACA GATGGATATC 
     1981 AGCGAGAAGC TGTTGGTGGC ATCACAATCA AGCTCCAATC TCCCGATGTC AAGTGGTTTG 
     2041 ATGATTATTA TCTGAAGCTC CGGCCAGAAA CAAACCACCG AAACCCTTGG TTTCAAGAAT 
     2101 TTTGGCAGCA TCGTTTTCAG TGCCGACTGG AAGGGTTTCC ACAGGAGAAC AGCAAATACA 
     2161 ACAAGACTTG CAATAGTTCT CTGACTCTGA AAACACATCA TGTTCAGGAT TCCAAAATGG 
     2221 GATTTGTGAT CAACGCCATC TATTCGATGG CCTATGGGCT CCACAACATG CAGATGTCCC 
     2281 TCTGCCCAGG CTATGCAGGA CTCTGTGATG CCATGAAGCC AATTGATGGA CGGAAACTTT 
     2341 TGGAGTCCCT GATGAAAACC AATTTTACTG GGGTTTCTGG AGATACGATC CTATTCGATG 
     2401 AGAATGGAGA CTCTCCAGGA AGGTATGAAA TAATGAATTT CAAGGAAATG GGAAAAGATT 
     2461 ACTTTGATTA TATCAACGTT GGAAGTTGGG ACAATGGAGA ATTAAAAATG GATGATGATG 
     2521 AAGTATGGTC CAAGAAAAGC AACATCATCA GATCTGTGTG CAGTGAACCA TGTGAGAAAG 
     2581 GCCAGATCAA GGTGATCCGA AAGGGAGAAG TCAGCTGTTG TTGGACCTGT ACACCTTGTA 
     2641 AGGAGAATGA GTATGTCTTT GATGAGTACA CATGCAAGGC ATGCCAACTG GGGTCTTGGC 
     2701 CCACTGATGA TCTCACAGGT TGTGACTTGA TCCCAGTACA GTATCTTCGA TGGGGTGACC 
     2761 CTGAACCCAT TGCAGCTGTG GTGTTTGCCT GCCTTGGCCT CCTGGCCACC CTGTTTGTTA 
     2821 CTGTAGTCTT CATCATTTAC CGTGATACAC CAGTAGTCAA GTCCTCAAGC AGGGAACTCT 
     2881 GCTACATTAT CCTTGCTGGC ATCTGCCTGG GCTACTTATG TACCTTCTGC CTCATTGCGA 
     2941 AGCCCAAACA GATTTACTGC TACCTTCAGA GAATTGGCAT TGGTCTCTCC CCAGCCATGA 
     3001 GCTACTCAGC CCTTGTAACA AAGACCAACC GTATTGCAAG GATCCTGGCT GGCAGCAAGA 
     3061 AGAAGATCTG TACCAAAAAG CCCAGATTCA TGAGTGCCTG TGCCCAGCTA GTGATTGCTT 
     3121 TCATTCTCAT ATGCATCCAG TTGGGCATCA TCGTTGCCCT CTTTATAATG GAGCCTCCTG 
     3181 ACATAATGCA TGACTACCCA AGCATTCGAG AAGTCTACCT GATCTGTAAC ACCACCAACC 
     3241 TAGGAGTTGT CACTCCACTT GGATACAATG GATTGTTGAT TTTGAGCTGC ACCTTCTATG 
     3301 CGTTCAAGAC CAGAAATGTT CCAGCTAACT TCAACGAGGC CAAGTATATC GCCTTCACAA 
     3361 TGTACACGAC CTGCATTATA TGGCTAGCTT TTGTGCCAAT CTACTTTGGC AGCAACTACA 
     3421 AAATCATCAC CATGTGTTTC TCGGTCAGCC TCAGTGCCAC AGTGGCCCTA GGCTGCATGT 
     3481 TTGTGCCGAA GGTGTACATC ATCCTGGCCA AACCAGAGAG AAACGTGCGC AGCGCCTTCA 
     3541 CCACATCTAC CGTGGTGCGC ATGCATGTAG GGGATGGCAA GTCATCCTCC GCAGCCAGCA 
     3601 GATCCAGCAG CCTAGTCAAC CTGTGGAAGA GAAGGGGCTC CTCTGGGGAA ACCTTAAGTT 
     3661 CCAATGGAAA ATCCGTCACG TGGGCCCAGA ATGAGAAGAG CAGCCGGGGG CAGCACCTGT 
     3721 GGCAGCGCCT GTCCATCCAC ATCAACAAGA AAGAAAACCC CAACCAAACG GCCGTCATCA 
     3781 AGCCCTTCCC CAAGAGCACG GAGAGCCGTG GCCTGGGCGC TGGCGCTGGC GCAGGCGGGA 
     3841 GCGCTGGGGG CGTGGGGGCC ACGGGCGGTG CGGGCTGCGC AGGCGCCGGC CCAGGCGGGC 
     3901 CCGAGTCCCC AGACGCCGGC CCCAAGGCGC TGTATGATGT GGCCGAGGCT GAGGAGCACT 
     3961 TCCCGGCGCC CGCGCGGCCG CGCTCACCGT CGCCCATCAG CACGCTGAGC CACCGCGCGG 
     4021 GCTCGGCCAG CCGCACGGAC GACGATGTGC CGTCGCTGCA CTCGGAGCCT GTGGCGCGCA 
     4081 GCAGCTCCTC GCAGGGCTCC CTCATGGAGC AGATCAGCAG TGTGGTCACC CGCTTCACGG 
     4141 CCAACATCAG CGAGCTCAAC TCCATGATGC TGTCCACCGC GGCCCCCAGC CCCGGCGTCG 
     4201 GCGCCCCGCT CTGCTCGTCC TACCTGATCC CCAAAGAGAT CCAGTTGCCC ACGACCATGA 
     4261 CGACCTTTGC CGAAATCCAG CCTCTGCCGG CCATCGAAGT CACGGGCGGC GCGCAGCCCG 
     4321 CGGCAGGGGC GCAGGCGGCT GGGGACGCGG CCCGGGAGAG CCCCGCGGCC GGTCCCGAGG 
     4381 CTGCGGCCGC CAAGCCAGAC CTGGAGGAGC TGGTGGCTCT CACCCCGCCG TCCCCCTTCA 
     4441 GAGACTCGGT GGACTCGGGG AGCACAACCC CCAACTCGCC AGTGTCCGAG TCGGCCCTCT 
     4501 GTATCCCGTC GTCTCCCAAA TATGACACTC TTATCATAAG AGATTACACT CAGAGCTCCT 
     4561 CGTCGTTGAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     4621 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     4681 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     4741 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4801 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     4861 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     4921 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     4981 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     5041 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     5101 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     5161 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     5221 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5281 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5341 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     5401 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     5461 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     5521 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     5581 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     5641 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     5701 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     5761 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     5821 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     5881 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     5941 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     6001 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     6061 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     6121 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     6181 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     6241 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     6301 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     6361 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     6421 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     6481 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     6541 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     6601 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     6661 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     6721 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     6781 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     6841 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     6901 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     6961 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     7021 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     7081 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     7141 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     7201 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     7261 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     7321 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     7381 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     7441 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     7501 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     7561 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     7621 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     7681 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7741 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7801 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     7861 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     7921 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     7981 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     8041 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     8101 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     8161 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     8221 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     8281 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     8341 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     8401 CAAAAATTTA ACGCGAATTT T
//