LOCUS       RC216843                8292 bp    DNA     circular  SYN 
DEFINITION  MAMDC4 (Myc-DDK-tagged)-Human MAM domain containing 4 (MAMDC4)
ACCESSION   NM_206920
VERSION     NM_206920.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4449..4540 
                     /label="Myc-DDK"  
     misc_feature    4570..5155 
                     /label="PolyA signal"  
     misc_feature    4627..4646 
                     /label="Restriction Enzyme"  
     misc_feature    5431..6103 
                     /label="ColE1"  
     misc_feature    6533..7327 
                     /label="Kan/Neo"  
     misc_feature    7362..7664 
                     /label="SV40 ori"  
     misc_feature    7750..8227 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4431..4437 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4431 
                     /label="MAMDC4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCTCTGTCC AGCCACCTGC TGCCCGCCTT GGTCCTGTTC CTGGCAGGGT 
     1081 CCTCAGGCTG GGCCTGGGTC CCCAACCACT GCAGGAGCCC TGGCCAGGCC GTGTGCAACT 
     1141 TCGTGTGTGA CTGCAGGGAC TGCTCAGATG AGGCCCAGTG TGGTTACCAC GGGGCCTCGC 
     1201 CCACCCTGGG CGCCCCCTTC GCCTGTGACT TCGAGCAGGA CCCCTGCGGC TGGCGGGACA 
     1261 TTAGTACCTC AGGCTACAGC TGGCTCCGAG ACAGGGCAGG GGCCGCACTG GAGGGTCCTG 
     1321 GGCCTCACTC AGACCACACA CTGGGCACCG ACTTGGGCTG GTACATGGCC GTTGGAACCC 
     1381 ACCGAGGGAA AGAGGCATCC ACCGCAGCCC TGCGCTCGCC AACCCTGCGA GAGGCAGCCT 
     1441 CCTCTTGCAA GCTGAGGCTC TGGTACCACG CGGCCTCTGG AGATGTGGCT GAACTGCGGG 
     1501 TGGAGCTGAC CCATGGCGCA GAGACCCTGA CCCTGTGGCA GAGCACAGGG CCCTGGGGCC 
     1561 CTGGCTGGCA GGAGTTGGCA GTGACCACAG GCCGCATCCG GGGTGACTTC CGAGTGACCT 
     1621 TCTCTGCCAC CCGAAATGCC ACCCACAGGG GCGCTGTGGC TCTAGATGAC CTAGAGTTCT 
     1681 GGGACTGTGG TCTGCCCACC CCCCAGGCCA ACTGTCCCCC GGGACACCAC CACTGCCAGA 
     1741 ACAAGGTCTG CGTGGAGCCC CAGCAGCTGT GCGACGGGGA AGACAACTGC GGGGACCTGT 
     1801 CTGATGAGAA CCCACTCACC TGTGGCCGCC ACATAGCCAC CGACTTTGAG ACAGGCCTGG 
     1861 GCCCATGGAA CCGCTCGGAA GGCTGGTCCC GGAACCACCG TGCTGGTGGT CCTGAGCGCC 
     1921 CCTCCTGGCC ACGCCGTGAC CACAGCCGGA ACAGTGCACA GGGCTCCTTC CTGGTCTCCG 
     1981 TGGCCGAGCC TGGCACCCCT GCTATACTCT CCAGCCCCGA ATTCCAAGCC TCAGGCACCT 
     2041 CCAACTGCTC GCTGGTCTTC TATCAGTACC TGAGTGGGTC TGAGGCTGGC TGCCTCCAGC 
     2101 TGTTCCTGCA GACTCTGGGG CCCGGCGCCC CCCGGGCCCC CGTCCTGCTG CGGAGGCGCC 
     2161 GAGGGGAGCT GGGGACCGCC TGGGTCCGAG ACCGTGTTGA CATCCAGAGC GCCTACCCCT 
     2221 TCCAGATCCT CCTGGCCGGG CAGACAGGCC CGGGGGGCGT CGTGGGTCTG GACGACCTCA 
     2281 TCCTGTCTGA CCACTGCAGA CCAGTCTCGG AGGTGTCCAC CCTGCAGCCG CTGCCTCCTG 
     2341 GGCCCCGGGC CCCAGCCCCC CAGCCCCTGC CGCCCAGCTC GCGGCTCCAG GATTCCTGCA 
     2401 AGCAGGGGCA TCTTGCCTGC GGGGACCTGT GTGTGCCCCC GGAACAACTG TGTGACTTCG 
     2461 AGGAGCAGTG CGCAGGGGGC GAGGACGAGC AGGCCTGTGG CACCACAGAC TTTGAGTCCC 
     2521 CCGAGGCTGG GGGCTGGGAG GACGCCAGCG TGGGGCGGCT GCAGTGGCGG CGTGTCTCAG 
     2581 CCCAGGAGAG CCAGGGGTCC AGTGCAGCTG CTGCTGGGCA CTTCCTGTCT CTGCAGCGGG 
     2641 CCTGGGGGCA GCTAGGCGCT GAGGCCCGGG TCCTCACACC CCTCCTTGGC CCTTCTGGCC 
     2701 CCAGCTGTGA ACTCCACCTG GCTTATTATT TACAGAGCCA GCCCCGAGAG GTCTCCTGTA 
     2761 ACTTTGAGCG GGACACATGC AGCTGGTACC CAGGCCACCT CTCAGACACA CACTGGCGCT 
     2821 GGGTGGAGAG CCGCGGCCCT GACCACGACC ACACCACAGG CCAAGGCCAC TTTGTGCTCC 
     2881 TGGACCCCAC AGACCCCCTG GCCTGGGGCC ACAGTGCCCA CCTGCTCTCC AGGCCCCAGG 
     2941 TGCCAGCAGC ACCCACGGAG TGTCTCAGCT TCTGGTACCA CCTCCATGGG CCCCAGATTG 
     3001 GGACTCTGCG CCTAGCCATG AGACGGGAAG GGGAGGAGAC ACACCTGTGG TCGCGGTCAG 
     3061 GCACCCAGGG CAACCGCTGG CACGAGGCCT GGGCCACCCT TTCCCACCAG CCTGGCTCCC 
     3121 ATGCCCAGTA CCAGCTGCTG TTCGAGGGCC TCCGGGACGG ATACCACGGC ACCATGGCGC 
     3181 TGGACGATGT GGCCGTGCGG CCGGGCCCCT GCTGGGCCCC TAATTACTGC TCCTTTGAGG 
     3241 ACTCAGACTG CGGCTTCTCC CCTGGAGGCC AAGGTCTCTG GAGGCGGCAG GCCAATGCCT 
     3301 CGGGCCATGC TGCCTGGGGC CCCCCAACAG ACCATACCAC TGAGACAGCC CAAGGGCACT 
     3361 ACATGGTGGT GGACACAAGC CCAGACGCAC TACCCCGGGG CCAGACGGCC TCCCTGACCT 
     3421 CCAAGGAGCA CAGGCCCCTG GCCCAGCCTG CTTGTCTGAC CTTCTGGTAC CACGGGAGCC 
     3481 TCCGCAGCCC AGGCACCCTG CGGGTCTACC TGGAGGAGCG CGGGAGGCAC CAGGTGCTCA 
     3541 GCCTCAGTGC CCACGGCGGG CTTGCCTGGC GCCTGGGCAG CATGGACGTG CAGGCCGAGC 
     3601 GAGCCTGGAG GGTGGTGTTT GAGGCAGTGG CCGCAGGCGT GGCACACTCC TACGTGGCTC 
     3661 TGGATGATCT GCTCCTCCAG GACGGGCCCT GCCCTCAGCC AGGTTCCTGT GATTTTGAGT 
     3721 CTGGCCTGTG TGGCTGGAGC CACCTGGCCG GGCCCGGCCT GGGCGGATAC AGCTGGGACT 
     3781 GGGGCGGGGG AGCCACCCCC TCTCGTTACC CCCAGCCCCC TGTGGACCAC ACCCTGGGCA 
     3841 CAGAGGCAGG CCACTTTGCC TTCTTTGAAA CTGGCGTGCT GGGCCCCGGG GGCCGGGCCG 
     3901 CCTGGCTGCG CAGCGAGCCT CTGCCGGCCA CCCCAGCCTC CTGCCTCCGC TTCTGGTACC 
     3961 ACATGGGTTT TCCTGAGCAC TTCTACAAGG GGGAGCTGAA GGTACTGCTG CACAGTGCTC 
     4021 AGGGCCAGCT GGCTGTGTGG GGCGCAGGCG GGCATCGGCG GCACCAGTGG CTGGAGGCCC 
     4081 AGGTGGAGGT AGCCAGTGCC AAGGAGTTCC AGATCGTGTT TGAAGCCACT CTGGGCGGCC 
     4141 AGCCAGCCCT GGGGCCCATT GCCCTGGATG ACGTGGAGTA TCTGGCTGGG CAGCATTGCC 
     4201 AGCAGCCTGC CCCCAGCCCG GGGAACACAG CCGCACCCGG GTCTGTGCCA GCTGTGGTTG 
     4261 GCAGTGCCCT CCTATTGCTC ATGCTCCTGG TGCTGCTGGG ACTTGGGGGA CGGCGCTGGC 
     4321 TGCAGAAGAA GGGGAGCTGC CCCTTCCAGA GCAACACAGA GGCCACAGCC CCTGGCTTTG 
     4381 ACAACATCCT TTTCAATGCG GATGGTGTCA CCCTCCCGGC ATCTGTCACC AGTGATCCGA 
     4441 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     4501 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     4561 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     4621 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     4681 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     4741 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     4801 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     4861 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     4921 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     4981 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     5041 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     5101 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     5161 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     5221 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     5281 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     5341 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     5401 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     5461 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     5521 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     5581 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     5641 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     5701 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     5761 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     5821 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     5881 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     5941 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     6001 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     6061 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     6121 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     6181 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     6241 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     6301 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     6361 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     6421 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     6481 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     6541 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     6601 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     6661 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     6721 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     6781 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     6841 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     6901 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     6961 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     7021 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     7081 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     7141 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     7201 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     7261 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     7321 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     7381 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     7441 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     7501 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     7561 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     7621 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7681 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     7741 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     7801 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     7861 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     7921 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     7981 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     8041 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     8101 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     8161 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     8221 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     8281 AACGCGAATT TT
//