LOCUS       RC216806                8109 bp    DNA     circular  SYN 
DEFINITION  NEMF (Myc-DDK-tagged)-Human nuclear export mediator factor (NEMF)
ACCESSION   NM_004713
VERSION     NM_004713.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4266..4357 
                     /label="Myc-DDK"  
     misc_feature    4387..4972 
                     /label="PolyA signal"  
     misc_feature    4444..4463 
                     /label="Restriction Enzyme"  
     misc_feature    5248..5920 
                     /label="ColE1"  
     misc_feature    6350..7144 
                     /label="Kan/Neo"  
     misc_feature    7179..7481 
                     /label="SV40 ori"  
     misc_feature    7567..8044 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4248..4254 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4248 
                     /label="NEMF"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGAGCCGC TTTAGCACCA TTGACCTCCG CGCCGTACTC GCGGAGCTGA 
     1081 ATGCTAGCTT GCTAGGAATG AGAGTAAACA ATGTTTATGA TGTGGATAAT AAGACATACC 
     1141 TTATTCGTCT TCAAAAACCG GACTTTAAAG CTACACTTTT ACTTGAATCT GGCATACGAA 
     1201 TTCATACAAC AGAATTTGAG TGGCCTAAGA ATATGATGCC GTCTAGTTTT GCCATGAAGT 
     1261 GCCGAAAACA TTTGAAGAGT CGGAGATTAG TCAGTGCAAA ACAGCTTGGT GTGGATAGAA 
     1321 TTGTAGATTT TCAATTTGGA AGTGATGAAG CTGCTTACCA TTTAATCATT GAGCTCTATG 
     1381 ATAGGGGGAA CATTGTTCTT ACAGATTATG AGTACGTAAT TTTAAATATT CTAAGGTTTC 
     1441 GAACTGATGA GGCAGATGAT GTTAAATTTG CTGTTCGTGA ACGCTATCCA CTTGATCATG 
     1501 CTAGAGCTGC TGAACCTTTG CTTACTTTGG AAAGGTTGAC TGAAATAGTA GCCAGCGCAC 
     1561 CTAAGGGTGA ACTACTGAAG AGGGTGCTTA ACCCATTACT TCCCTATGGA CCAGCTCTCA 
     1621 TTGAACACTG TCTTTTAGAA AATGGATTCT CGGGTAATGT CAAAGTGGAT GAAAAACTTG 
     1681 AAACTAAAGA TATTGAAAAA GTACTTGTTT CTCTGCAGAA AGCAGAAGAC TATATGAAAA 
     1741 CAACATCCAA CTTCAGTGGG AAGGGATATA TCATTCAGAA AAGAGAAATA AAACCATGCT 
     1801 TGGAAGCAGA TAAACCAGTT GAAGACATAC TGACGTATGA GGAATTTCAT CCTTTCTTGT 
     1861 TTTCTCAACA TTCACAATGT CCATATATAG AATTTGAATC ATTTGACAAG GCGGTGGATG 
     1921 AATTTTATTC CAAGATAGAA GGCCAGAAAA TTGACTTAAA AGCTTTACAA CAGGAAAAGC 
     1981 AAGCATTGAA GAAATTAGAT AATGTTCGAA AGGATCACGA AAACAGATTG GAAGCTCTTC 
     2041 AGCAGGCTCA GGAAATAGAC AAACTGAAAG GAGAGCTCAT AGAAATGAAC CTACAAATAG 
     2101 TTGACAGAGC CATTCAGGTA GTTCGAAGTG CTTTAGCTAA CCAGATAGAT TGGACAGAAA 
     2161 TTGGGTTAAT TGTGAAAGAA GCCCAGGCTC AAGGAGACCC TGTTGCAAGT GCAATCAAAG 
     2221 AATTAAAACT ACAAACAAAC CATGTTACAA TGCTGCTAAG AAATCCATAC TTGTTATCAG 
     2281 AGGAGGAAGA TGATGATGTT GATGGTGACG TCAATGTTGA GAAAAATGAA ACTGAACCAC 
     2341 CAAAAGGAAA AAAGAAAAAA CAAAAGAATA AACAGCTGCA GAAGCCTCAG AAAAATAAGC 
     2401 CCTTACTTGT AGATGTTGAT CTCAGCTTGT CAGCATATGC CAATGCCAAA AAGTATTATG 
     2461 ATCACAAGAG ATATGCTGCT AAGAAAACAC AAAAGACTGT TGAAGCTGCT GAGAAGGCAT 
     2521 TCAAGTCAGC AGAAAAGAAA ACAAAGCAAA CATTAAAAGA AGTTCAGACT GTTACCTCTA 
     2581 TTCAAAAAGC AAGAAAAGTA TATTGGTTTG AGAAATTTCT GTGGTTCATT AGCTCAGAGA 
     2641 ACTATCTAAT TATAGGTGGA CGAGATCAGC AACAGAATGA AATAATTGTG AAAAGATACT 
     2701 TGACACCAGG AGACATTTAT GTACATGCTG ATCTTCATGG AGCTACTAGC TGTGTAATTA 
     2761 AGAATCCAAC AGGAGAACCC ATCCCCCCAC GGACCTTGAC TGAAGCTGGC ACAATGGCAC 
     2821 TTTGCTACAG TGCTGCTTGG GATGCACGAG TTATCACTAG TGCTTGGTGG GTGTACCATC 
     2881 ATCAGGTATC TAAAACAGCA CCAACTGGAG AATATTTGAC AACAGGAAGC TTCATGATAA 
     2941 GAGGAAAAAA GAATTTTCTT CCTCCCTCAT ATCTAATGAT GGGGTTTAGC TTCCTTTTTA 
     3001 AGGTAGATGA GTCTTGTGTT TGGAGACATC AGGGTGAACG AAAAGTCAGA GTACAGGATG 
     3061 AAGACATGGA GACACTGGCA AGTTGTACAA GTGAACTCAT ATCAGAAGAA ATGGAACAAT 
     3121 TAGATGGAGG TGACACGAGC AGTGATGAGG ATAAAGAAGA ACATGAAACT CCTGTGGAAG 
     3181 TAGAACTCAT GACTCAGGTT GACCAAGAGG ATATCACTCT TCAGAGTGGC AGAGATGAAC 
     3241 TAAATGAGGA GCTCATTCAG GAAGAAAGCT CTGAAGACGA AGGAGAATAT GAAGAGGTTA 
     3301 GAAAAGATCA GGATTCTGTT GGTGAAATGA AGGATGAAGG GGAAGAGACA TTAAATTATC 
     3361 CTGATACTAC CATTGACTTG TCTCACCTTC AACCCCAAAG GTCCATCCAG AAATTGGCTT 
     3421 CAAAAGAGGA ATCTTCTAAT TCTAGTGACA GTAAATCACA GAGCCGGAGA CATTTGTCAG 
     3481 CCAAGGAAAG AAGGGAAATG AAAAAGAAAA AACTTCCAAG TGACTCAGGA GATTTAGAAG 
     3541 CGTTAGAGGG AAAGGATAAA GAAAAAGAAA GTACTGTACA CATTGAAACT CATCAGAACA 
     3601 CAAGCAAAAA TGTTGCGGCT GTGCAGCCAA TGAAACGAGG ACAAAAGAGT AAAATGAAAA 
     3661 AAATGAAAGA AAAATACAAA GACCAGGATG AAGAAGACCG TGAACTTATC ATGAAGTTGC 
     3721 TGGGGTCTGC AGGTTCAAAC AAAGAAGAAA AAGGGAAGAA GGGGAAGAAA GGAAAAACAA 
     3781 AGGACGAACC TGTGAAGAAA CAGCCCCAGA AACCTAGAGG TGGACAGAGG GTCTCTGACA 
     3841 ACATTAAGAA AGAAACTCCG TTCCTTGAGG TTATAACTCA TGAGTTACAA GACTTTGCTG 
     3901 TAGATGATCC ACATGATGAC AAGGAAGAGC AAGATCTGGA TCAACAGGGA AATGAGGAAA 
     3961 ACCTATTTGA TTCTTTGACA GGCCAGCCAC ATCCTGAAGA TGTACTACTG TTTGCCATTC 
     4021 CAATATGTGC CCCTTACACC ACCATGACAA ACTACAAATA TAAAGTGAAA CTTACTCCTG 
     4081 GAGTGCAGAA AAAGGGAAAA GCTGCAAAAA CAGCCTTGAA TAGTTTCATG CATTCCAAAG 
     4141 AAGCAACAGC AAGAGAAAAA GACTTATTCC GCAGCGTAAA GGACACAGAT TTATCAAGAA 
     4201 ACATTCCTGG CAAAGTGAAA GTGTCTGCAC CCAATCTTCT GAACGTAAAA AGGAAAACGC 
     4261 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4321 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4381 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4441 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4501 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4561 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     4621 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     4681 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     4741 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     4801 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     4861 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     4921 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     4981 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5041 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5101 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5161 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5221 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5281 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5341 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5401 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     5461 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     5521 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     5581 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     5641 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     5701 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     5761 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     5821 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     5881 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     5941 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6001 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6061 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6121 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6181 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6241 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6301 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6361 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6421 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     6481 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     6541 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     6601 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     6661 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     6721 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     6781 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     6841 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     6901 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     6961 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7021 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7081 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7141 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7201 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7261 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7321 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7381 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7441 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7501 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     7561 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     7621 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     7681 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     7741 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     7801 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     7861 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     7921 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     7981 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8041 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8101 GCGAATTTT
//