LOCUS       RC216796L2                9309 bp    DNA     circular  SYN 
DEFINITION  ASPH (Myc-DDK-tagged)-Human aspartate beta-hydroxylase (ASPH), transcript variant 1
ACCESSION   NM_004318
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7600..7780 
                     /label="3-LTR"  
     misc_feature    833..1013 
                     /label="5-LTR"  
     misc_feature    8508..9167 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV"  
     misc_feature    7828..8447 
                     /label="ColE1"  
     misc_feature    6475..6495 
                     /label="LR50"  
     misc_feature    5615..6340 
                     /label="mGFP"  
     misc_feature    3252..3271 
                     /label="T7"  
     misc_feature    6484..7075 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5594..5600 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5594 
                     /label="ASPH"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGCCCAGCGT AAGAATGCCA AGAGCAGCGG 
     3361 CAACAGCAGC AGCAGCGGCT CCGGCAGCGG TAGCACGAGT GCGGGCAGCA GCAGCCCCGG 
     3421 GGCCCGGAGA GAGACAAAGC ATGGAGGACA CAAGAATGGG AGGAAAGGCG GACTCTCAGG 
     3481 AACTTCATTC TTCACGTGGT TTATGGTGAT TGCATTGCTG GGCGTCTGGA CATCTGTAGC 
     3541 TGTCGTTTGG TTTGATCTTG TTGACTATGA GGAAGTTCTA GGAAAACTAG GAATCTATGA 
     3601 TGCTGATGGT GATGGAGATT TTGATGTGGA TGATGCCAAA GTTTTATTAG GACTTAAAGA 
     3661 GAGATCTACT TCAGAGCCAG CAGTCCCGCC AGAAGAGGCT GAGCCACACA CTGAGCCCGA 
     3721 GGAGCAGGTT CCTGTGGAGG CAGAACCCCA GAATATCGAA GATGAAGCAA AAGAACAAAT 
     3781 TCAGTCCCTT CTCCATGAAA TGGTACACGC AGAACATGTT GAGGGAGAAG ACTTGCAACA 
     3841 AGAAGATGGA CCCACAGGAG AACCACAACA AGAGGATGAT GAGTTTCTTA TGGCGACTGA 
     3901 TGTAGATGAT AGATTTGAGA CCCTGGAACC TGAAGTATCT CATGAAGAAA CCGAGCATAG 
     3961 TTACCACGTG GAAGAGACAG TTTCACAAGA CTGTAATCAG GATATGGAAG AGATGATGTC 
     4021 TGAGCAGGAA AATCCAGATT CCAGTGAACC AGTAGTAGAA GATGAAAGAT TGCACCATGA 
     4081 TACAGATGAT GTAACATACC AAGTCTATGA GGAACAAGCA GTATATGAAC CTCTAGAAAA 
     4141 TGAAGGGATA GAAATCACAG AAGTAACTGC TCCCCCTGAG GATAATCCTG TAGAAGATTC 
     4201 ACAGGTAATT GTAGAAGAAG TAAGCATTTT TCCTGTGGAA GAACAGCAGG AAGTACCACC 
     4261 AGAAACAAAT AGAAAAACAG ATGATCCAGA ACAAAAAGCA AAAGTTAAGA AAAAGAAGCC 
     4321 TAAACTTTTA AATAAATTTG ATAAGACTAT TAAAGCTGAA CTTGATGCTG CAGAAAAACT 
     4381 CCGTAAAAGG GGAAAAATTG AGGAAGCAGT GAATGCATTT AAAGAACTAG TACGCAAATA 
     4441 CCCTCAGAGT CCACGAGCAA GATATGGGAA GGCGCAGTGT GAGGATGATT TGGCTGAGAA 
     4501 GAGGAGAAGT AATGAGGTGC TACGTGGAGC CATCGAGACC TACCAAGAGG TGGCCAGCCT 
     4561 ACCTGATGTC CCTGCAGACC TGCTGAAGCT GAGTTTGAAG CGTCGCTCAG ACAGGCAACA 
     4621 ATTTCTAGGT CATATGAGAG GTTCCCTGCT TACCCTGCAG AGATTAGTTC AACTATTTCC 
     4681 CAATGATACT TCCTTAAAAA ATGACCTTGG CGTGGGATAC CTCTTGATAG GAGATAATGA 
     4741 CAATGCAAAG AAAGTTTATG AAGAGGTGCT GAGTGTGACA CCTAATGATG GCTTTGCTAA 
     4801 AGTCCATTAT GGCTTCATCC TGAAGGCACA GAACAAAATT GCTGAGAGCA TCCCATATTT 
     4861 AAAGGAAGGA ATAGAATCCG GAGATCCTGG CACTGATGAT GGGAGATTTT ATTTCCACCT 
     4921 GGGGGATGCC ATGCAGAGGG TTGGGAACAA AGAGGCATAT AAGTGGTATG AGCTTGGGCA 
     4981 CAAGAGAGGA CACTTTGCAT CTGTCTGGCA ACGCTCACTC TACAATGTGA ATGGACTGAA 
     5041 AGCACAGCCT TGGTGGACCC CAAAAGAAAC GGGCTACACA GAGTTAGTAA AGTCTTTAGA 
     5101 AAGAAACTGG AAGTTAATCC GAGATGAAGG CCTTGCAGTG ATGGATAAAG CCAAAGGTCT 
     5161 CTTCCTGCCT GAGGATGAAA ACCTGAGGGA AAAAGGGGAC TGGAGCCAGT TCACGCTGTG 
     5221 GCAGCAAGGA AGAAGAAATG AAAATGCCTG CAAAGGAGCT CCTAAAACCT GTACCTTACT 
     5281 AGAAAAGTTC CCCGAGACAA CAGGATGCAG AAGAGGACAG ATCAAATATT CCATCATGCA 
     5341 CCCCGGGACT CACGTGTGGC CGCACACAGG GCCCACAAAC TGCAGGTTCC GAATGCACCT 
     5401 GGGCTTGGTG ATTCCCAAGG AAGGCTGCAA GATTCGATGT GCCAACGAGA CCAAGACCTG 
     5461 GGAGGAAGGC AAGGTGCTCA TCTTTGATGA CTCCTTTGAG CACGAGGTAT GGCAGGATGC 
     5521 CTCATCTTTC CGGCTGATAT TCATCGTGGA TGTGTGGCAT CCGGAACTGA CACCACAGCA 
     5581 GAGACGCAGC CTTCCAGCAA TTACGCGTAC GCGGCCGCTC GAGATGAGCG GGGGCGAGGA 
     5641 GCTGTTCGCC GGCATCGTGC CCGTGCTGAT CGAGCTGGAC GGCGACGTGC ACGGCCACAA 
     5701 GTTCAGCGTG CGCGGCGAGG GCGAGGGCGA CGCCGACTAC GGCAAGCTGG AGATCAAGTT 
     5761 CATCTGCACC ACCGGCAAGC TGCCCGTGCC CTGGCCCACC CTGGTGACCA CCCTCTGCTA 
     5821 CGGCATCCAG TGCTTCGCCC GCTACCCCGA GCACATGAAG ATGAACGACT TCTTCAAGAG 
     5881 CGCCATGCCC GAGGGCTACA TCCAGGAGCG CACCATCCAG TTCCAGGACG ACGGCAAGTA 
     5941 CAAGACCCGC GGCGAGGTGA AGTTCGAGGG CGACACCCTG GTGAACCGCA TCGAGCTGAA 
     6001 GGGCAAGGAC TTCAAGGAGG ACGGCAACAT CCTGGGCCAC AAGCTGGAGT ACAGCTTCAA 
     6061 CAGCCACAAC GTGTACATCC GCCCCGACAA GGCCAACAAC GGCCTGGAGG CTAACTTCAA 
     6121 GACCCGCCAC AACATCGAGG GCGGCGGCGT GCAGCTGGCC GACCACTACC AGACCAACGT 
     6181 GCCCCTGGGC GACGGCCCCG TGCTGATCCC CATCAACCAC TACCTGAGCA CTCAGACCAA 
     6241 GATCAGCAAG GACCGCAACG AGGCCCGCGA CCACATGGTG CTCCTGGAGT CCTTCAGCGC 
     6301 CTGCTGCCAC ACCCACGGCA TGGACGAGCT GTACAGGTCC GGACTCAGAT AAGTTTAAAC 
     6361 GGCCGGCCGC GGTCTGTACA AGTAGGATTC GTCGAGGGAC CTAATAACTT CGTATAGCAT 
     6421 ACATTATACG AAGTTATACA TGTTTAAGGG TTCCGGTTCC ACTAGGTACA ATTCGATATC 
     6481 AAGCTTATCG ATAATCAACC TCTGGATTAC AAAATTTGTG AAAGATTGAC TGGTATTCTT 
     6541 AACTATGTTG CTCCTTTTAC GCTATGTGGA TACGCTGCTT TAATGCCTTT GTATCATGCT 
     6601 ATTGCTTCCC GTATGGCTTT CATTTTCTCC TCCTTGTATA AATCCTGGTT GCTGTCTCTT 
     6661 TATGAGGAGT TGTGGCCCGT TGTCAGGCAA CGTGGCGTGG TGTGCACTGT GTTTGCTGAC 
     6721 GCAACCCCCA CTGGTTGGGG CATTGCCACC ACCTGTCAGC TCCTTTCCGG GACTTTCGCT 
     6781 TTCCCCCTCC CTATTGCCAC GGCGGAACTC ATCGCCGCCT GCCTTGCCCG CTGCTGGACA 
     6841 GGGGCTCGGC TGTTGGGCAC TGACAATTCC GTGGTGTTGT CGGGGAAATC ATCGTCCTTT 
     6901 CCTTGGCTGC TCGCCTGTGT TGCCACCTGG ATTCTGCGCG GGACGTCCTT CTGCTACGTC 
     6961 CCTTCGGCCC TCAATCCAGC GGACCTTCCT TCCCGCGGCC TGCTGCCGGC TCTGCGGCCT 
     7021 CTTCCGCGTC TTCGCCTTCG CCCTCAGACG AGTCGGATCT CCCTTTGGGC CGCCTCCCCG 
     7081 CATCGATACC GTCGACCTCG ATCGAGACCT AGAAAAACAT GGAGCAATCA CAAGTAGCAA 
     7141 TACAGCAGCT ACCAATGCTG ATTGTGCCTG GCTAGAAGCA CAAGAGGAGG AGGAGGTGGG 
     7201 TTTTCCAGTC ACACCTCAGG TACCTTTAAG ACCAATGACT TACAAGGCAG CTGTAGATCT 
     7261 TAGCCACTTT TTAAAAGAAA AGGGGGGACT GGAAGGGCTA ATTCACTCCC AACGAAGACA 
     7321 AGATATCCTT GATCTGTGGA TCTACCACAC ACAAGGCTAC TTCCCTGATT GGCAGAACTA 
     7381 CACACCAGGG CCAGGGATCA GATATCCACT GACCTTTGGA TGGTGCTACA AGCTAGTACC 
     7441 AGTTGAGCAA GAGAAGGTAG AAGAAGCCAA TGAAGGAGAG AACACCCGCT TGTTACACCC 
     7501 TGTGAGCCTG CATGGGATGG ATGACCCGGA GAGAGAAGTA TTAGAGTGGA GGTTTGACAG 
     7561 CCGCCTAGCA TTTCATCACA TGGCCCGAGA GCTGCATCCG GACTGTACTG GGTCTCTCTG 
     7621 GTTAGACCAG ATCTGAGCCT GGGAGCTCTC TGGCTAACTA GGGAACCCAC TGCTTAAGCC 
     7681 TCAATAAAGC TTGCCTTGAG TGCTTCAAGT AGTGTGTGCC CGTCTGTTGT GTGACTCTGG 
     7741 TAACTAGAGA TCCCTCAGAC CCTTTTAGTC AGTGTGGAAA ATCTCTAGCA GCATGTGAGC 
     7801 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     7861 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     7921 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     7981 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     8041 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     8101 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     8161 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     8221 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     8281 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     8341 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     8401 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     8461 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGATTAC 
     8521 GCCCCGCCCT GCCACTCATC GCAGTACTGT TGTAATTCAT TAAGCATTCT GCCGACATGG 
     8581 AAGCCATCAC AAACGGCATG ATGAACCTGA ATCGCCAGCG GCATCAGCAC CTTGTCGCCT 
     8641 TGCGTATAAT ATTTGCCCAT GGTGAAAACG GGGGCGAAGA AGTTGTCCAT ATTGGCCACG 
     8701 TTTAAATCAA AACTGGTGAA ACTCACCCAG GGATTGGCTG AGACGAAAAA CATATTCTCA 
     8761 ATAAACCCTT TAGGGAAATA GGCCAGGTTT TCACCGTAAC ACGCCACATC TTGCGAATAT 
     8821 ATGTGTAGAA ACTGCCGGAA ATCGTCGTGG TATTCACTCC AGAGCGATGA AAACGTTTCA 
     8881 GTTTGCTCAT GGAAAACGGT GTAACAAGGG TGAACACTAT CCCATATCAC CAGCTCACCG 
     8941 TCTTTCATTG CCATACGGAA CTCCGGATGA GCATTCATCA GGCGGGCAAG AATGTGAATA 
     9001 AAGGCCGGAT AAAACTTGTG CTTATTTTTC TTTACGGTCT TTAAAAAGGC CGTAATATCC 
     9061 AGCTGAACGG TCTGGTTATA GGTACATTGA GCAACTGACT GAAATGCCTC AAAATGTTCT 
     9121 TTACGATGCC ATTGGGATAT ATCAACGGTG GTATATCCAG TGATTTTTTT CTCCATACTC 
     9181 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     9241 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     9301 CCACCTGAC
//