LOCUS       RC216770                8475 bp    DNA     circular  SYN 
DEFINITION  HCN4 (Myc-DDK-tagged)-Human hyperpolarization activated cyclic nucleotide-gated potassium channel 4 (HCN4)
ACCESSION   NM_005477
VERSION     NM_005477.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4632..4723 
                     /label="Myc-DDK"  
     misc_feature    4753..5338 
                     /label="PolyA signal"  
     misc_feature    4810..4829 
                     /label="Restriction Enzyme"  
     misc_feature    5614..6286 
                     /label="ColE1"  
     misc_feature    6716..7510 
                     /label="Kan/Neo"  
     misc_feature    7545..7847 
                     /label="SV40 ori"  
     misc_feature    7933..8410 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4629..4635 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4629 
                     /label="HCN4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGACAAGCTG CCGCCGTCCA TGCGCAAGCG GCTCTACAGC CTCCCGCAGC 
     1081 AGGTGGGGGC CAAGGCGTGG ATCATGGACG AGGAAGAGGA CGCCGAGGAG GAGGGGGCCG 
     1141 GGGGCCGCCA AGACCCCAGC CGCAGGAGCA TCCGGCTGCG GCCACTGCCC TCGCCCTCCC 
     1201 CCTCGGCGGC CGCGGGTGGC ACGGAGTCCC GGAGCTCGGC CCTCGGGGCA GCGGACAGCG 
     1261 AAGGGCCGGC CCGCGGCGCG GGCAAGTCCA GCACGAACGG CGACTGCAGG CGCTTCCGCG 
     1321 GGAGCCTGGC CTCGCTGGGC AGCCGGGGCG GCGGCAGCGG CGGCACGGGG AGCGGCAGCA 
     1381 GTCACGGACA CCTGCATGAC TCCGCGGAGG AGCGGCGGCT CATCGCCGAG GGCGACGCGT 
     1441 CCCCCGGCGA GGACAGGACG CCCCCAGGCC TGGCGGCCGA GCCCGAGCGC CCCGGCGCCT 
     1501 CGGCGCAGCC CGCAGCCTCG CCGCCGCCGC CCCAGCAGCC ACCGCAGCCG GCCTCCGCCT 
     1561 CCTGCGAGCA GCCCTCGGTG GACACCGCTA TCAAAGTGGA GGGAGGCGCG GCTGCCGGCG 
     1621 ACCAGATCCT CCCGGAGGCC GAGGTGCGCC TGGGCCAGGC CGGCTTCATG CAGCGCCAGT 
     1681 TCGGGGCCAT GCTCCAACCC GGGGTCAACA AATTCTCCCT AAGGATGTTC GGCAGCCAGA 
     1741 AAGCCGTGGA GCGCGAACAG GAGAGGGTCA AGTCGGCCGG ATTTTGGATT ATCCACCCCT 
     1801 ACAGTGACTT CAGATTTTAC TGGGACCTGA CCATGCTGCT GCTGATGGTG GGAAACCTGA 
     1861 TTATCATTCC TGTGGGCATC ACCTTCTTCA AGGATGAGAA CACCACACCC TGGATTGTCT 
     1921 TCAATGTGGT GTCAGACACA TTCTTCCTCA TCGACTTGGT CCTCAACTTC CGCACAGGGA 
     1981 TCGTGGTGGA GGACAACACA GAGATCATCC TGGACCCGCA GCGGATTAAA ATGAAGTACC 
     2041 TGAAAAGCTG GTTCATGGTA GATTTCATTT CCTCCATCCC CGTGGACTAC ATCTTCCTCA 
     2101 TTGTGGAGAC ACGCATCGAC TCGGAGGTCT ACAAGACTGC CCGGGCCCTG CGCATTGTCC 
     2161 GCTTCACGAA GATCCTCAGC CTCTTACGCC TGTTACGCCT CTCCCGCCTC ATTCGATATA 
     2221 TTCACCAGTG GGAAGAGATC TTCCACATGA CCTACGACCT GGCCAGCGCC GTGGTGCGCA 
     2281 TCGTGAACCT CATCGGCATG ATGCTCCTGC TCTGCCACTG GGACGGCTGC CTGCAGTTCC 
     2341 TGGTACCCAT GCTACAGGAC TTCCCTGACG ACTGCTGGGT GTCCATCAAC AACATGGTGA 
     2401 ACAACTCCTG GGGGAAGCAG TACTCCTACG CGCTCTTCAA GGCCATGAGC CACATGCTGT 
     2461 GCATCGGCTA CGGGCGGCAG GCGCCCGTGG GCATGTCCGA CGTCTGGCTC ACCATGCTCA 
     2521 GCATGATCGT GGGTGCCACC TGCTACGCCA TGTTCATTGG CCACGCCACT GCCCTCATCC 
     2581 AGTCCCTGGA CTCCTCCCGG CGCCAGTACC AGGAAAAGTA CAAGCAGGTG GAGCAGTACA 
     2641 TGTCCTTTCA CAAGCTCCCG CCCGACACCC GGCAGCGCAT CCACGACTAC TACGAGCACC 
     2701 GCTACCAGGG CAAGATGTTC GACGAGGAGA GCATCCTGGG CGAGCTAAGC GAGCCCCTGC 
     2761 GGGAGGAGAT CATCAACTTT AACTGTCGGA AGCTGGTGGC CTCCATGCCA CTGTTTGCCA 
     2821 ATGCGGACCC CAACTTCGTG ACGTCCATGC TGACCAAGCT GCGTTTCGAG GTCTTCCAGC 
     2881 CTGGGGACTA CATCATCCGG GAAGGCACCA TTGGCAAGAA GATGTACTTC ATCCAGCATG 
     2941 GCGTGGTCAG CGTGCTCACC AAGGGCAACA AGGAGACCAA GCTGGCCGAC GGCTCCTACT 
     3001 TTGGAGAGAT CTGCCTGCTG ACCCGGGGCC GGCGCACAGC CAGCGTGAGG GCCGACACCT 
     3061 ACTGCCGCCT CTACTCGCTG AGCGTGGACA ACTTCAATGA GGTGCTGGAG GAGTACCCCA 
     3121 TGATGCGAAG GGCCTTCGAG ACCGTGGCGC TGGACCGCCT GGACCGCATT GGCAAGAAGA 
     3181 ACTCCATCCT CCTCCACAAA GTCCAGCACG ACCTCAACTC CGGCGTCTTC AACTACCAGG 
     3241 AGAATGAGAT CATCCAGCAG ATTGTGCAGC ATGACCGGGA GATGGCCCAC TGCGCGCACC 
     3301 GCGTCCAGGC TGCTGCCTCT GCCACCCCAA CCCCCACGCC CGTCATCTGG ACCCCGCTGA 
     3361 TCCAGGCACC ACTGCAGGCT GCCGCTGCCA CCACTTCTGT GGCCATAGCC CTCACCCACC 
     3421 ACCCTCGCCT GCCTGCTGCC ATCTTCCGCC CTCCCCCAGG ATCTGGGCTG GGCAACCTCG 
     3481 GTGCCGGGCA GACGCCAAGG CACCTGAAAC GGCTGCAGTC CCTGATCCCT TCTGCGCTGG 
     3541 GCTCCGCCTC GCCCGCCAGC AGCCCGTCCC AGGTGGACAC ACCGTCTTCA TCCTCCTTCC 
     3601 ACATCCAACA GCTGGCTGGA TTCTCTGCCC CCGCTGGACT GAGCCCACTC CTGCCCTCAT 
     3661 CCAGCTCCTC CCCACCCCCC GGGGCCTGTG GCTCCCCCTC GGCTCCCACA CCATCAGCTG 
     3721 GCGTAGCCGC CACCACCATA GCCGGGTTTG GCCACTTCCA CAAGGCGCTG GGTGGCTCCC 
     3781 TGTCCTCCTC CGACTCTCCC CTGCTCACCC CGCTGCAGCC AGGCGCCCGC TCCCCGCAGG 
     3841 CTGCCCAGCC ATCTCCCGCG CCACCCGGGG CCCGGGGAGG CCTGGGACTC CCGGAGCACT 
     3901 TCCTGCCACC CCCACCCTCA TCCAGATCCC CGTCATCTAG CCCCGGGCAG CTGGGCCAGC 
     3961 CTCCCGGGGA GTTGTCCCTA GGTCTGGCCA CTGGCCCACT GAGCACGCCA GAGACACCCC 
     4021 CACGGCAGCC TGAGCCGCCG TCCCTTGTGG CAGGGGCCTC TGGGGGGGCT TCCCCTGTAG 
     4081 GCTTTACTCC CCGAGGAGGT CTCAGCCCCC CTGGCCACAG CCCAGGCCCC CCAAGAACCT 
     4141 TCCCGAGTGC CCCGCCCCGG GCCTCTGGCT CCCACGGATC CTTGCTCCTG CCACCTGCAT 
     4201 CCAGCCCCCC ACCACCCCAG GTCCCCCAGC GCCGGGGCAC ACCCCCGCTC ACCCCCGGCC 
     4261 GCCTCACCCA GGACCTCAAG CTCATCTCCG CGTCTCAGCC AGCCCTGCCT CAGGACGGGG 
     4321 CGCAGACTCT CCGCAGAGCC TCCCCGCACT CCTCAGGGGA GTCCATGGCT GCCTTCCCGC 
     4381 TCTTCCCCAG GGCTGGGGGT GGCAGCGGGG GCAGTGGGAG CAGCGGGGGC CTCGGTCCCC 
     4441 CTGGGAGGCC CTATGGTGCC ATCCCCGGCC AGCACGTCAC TCTGCCTCGG AAGACATCCT 
     4501 CAGGTTCTTT GCCACCCCCT CTGTCTTTGT TTGGGGCAAG AGCCACCTCT TCTGGGGGGC 
     4561 CCCCTCTGAC TGCTGGACCC CAGAGGGAAC CTGGGGCCAG GCCTGAGCCA GTGCGCTCCA 
     4621 AACTGCCATC CAATCTACTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     4681 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     4741 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     4801 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     4861 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     4921 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     4981 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     5041 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     5101 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     5161 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     5221 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     5281 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     5341 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     5401 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     5461 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     5521 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     5581 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     5641 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     5701 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     5761 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     5821 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     5881 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     5941 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     6001 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     6061 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     6121 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     6181 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     6241 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     6301 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     6361 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     6421 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     6481 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     6541 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     6601 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     6661 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     6721 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     6781 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     6841 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     6901 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     6961 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     7021 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     7081 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     7141 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     7201 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     7261 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     7321 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     7381 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     7441 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     7501 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     7561 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     7621 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     7681 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     7741 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     7801 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7861 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     7921 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     7981 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     8041 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     8101 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     8161 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     8221 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     8281 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     8341 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     8401 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     8461 TTTAACGCGA ATTTT
//