LOCUS       RC216742                8703 bp    DNA     circular  SYN 
DEFINITION  SYMPK (Myc-DDK-tagged)-Human symplekin (SYMPK)
ACCESSION   NM_004819
VERSION     NM_004819.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4860..4951 
                     /label="Myc-DDK"  
     misc_feature    4981..5566 
                     /label="PolyA signal"  
     misc_feature    5038..5057 
                     /label="Restriction Enzyme"  
     misc_feature    5842..6514 
                     /label="ColE1"  
     misc_feature    6944..7738 
                     /label="Kan/Neo"  
     misc_feature    7773..8075 
                     /label="SV40 ori"  
     misc_feature    8161..8638 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4842..4848 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4842 
                     /label="SYMPK"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGAGCGGC AGTGGAGACA GCGTCACCCG TCGGAGCGTG GCATCACAGT 
     1081 TTTTCACTCA AGAGGAGGGG CCGGGCATCG ATGGCATGAC CACCTCAGAG AGGGTGGTGG 
     1141 ATCTTCTGAA CCAGGCGGCG CTGATCACCA ATGACTCAAA GATCACAGTG CTCAAACAGG 
     1201 TCCAGGAGCT GATCATCAAC AAAGACCCCA CACTACTGGA CAACTTCCTG GATGAGATCA 
     1261 TCGCATTCCA AGCAGACAAG TCAATCGAAG TGCGAAAATT TGTCATCGGC TTCATCGAGG 
     1321 AGGCATGCAA GCGAGACATT GAGTTGCTGC TGAAACTCAT TGCAAACCTC AACATGCTCT 
     1381 TGAGGGACGA GAATGTGAAC GTGGTGAAGA AGGCTATCCT CACCATGACC CAGCTCTACA 
     1441 AGGTGGCCCT GCAGTGGATG GTAAAGTCAC GGGTCATTAG CGAGCTACAG GAGGCCTGCT 
     1501 GGGACATGGT ATCTGCCATG GCGGGGGACA TCATCCTGCT ATTGGACTCT GACAATGACG 
     1561 GCATCCGCAC CCACGCCATC AAGTTTGTGG AGGGCCTCAT TGTCACCCTG TCACCCCGCA 
     1621 TGGCTGACTC AGAGATACCC CGACGCCAGG AGCATGATAT CAGCCTGGAC CGCATCCCTC 
     1681 GTGACCACCC CTACATCCAG TACAACGTGC TATGGGAAGA GGGCAAGGCA GCCTTGGAGC 
     1741 AGCTGCTTAA GTTCATGGTG CACCCTGCCA TCTCCTCCAT CAACCTGACC ACAGCGCTGG 
     1801 GCTCCCTTGC CAATATCGCC CGCCAGAGAC CCATGTTCAT GTCTGAGGTG ATCCAGGCCT 
     1861 ATGAAACTCT GCATGCCAAC CTGCCCCCGA CGCTGGCCAA ATCGCAGGTG AGCAGTGTGC 
     1921 GTAAGAATCT GAAGCTGCAC CTGTTGAGTG TGCTGAAGCA CCCGGCTTCC TTGGAGTTCC 
     1981 AGGCCCAGAT CACCACCCTG CTGGTGGACC TGGGCACACC TCAGGCCGAG ATCGCCCGCA 
     2041 ACATGCCGAG CAGCAAGGAC ACCCGCAAGC GGCCCCGCGA TGACTCGGAC TCCACACTCA 
     2101 AGAAGATGAA GCTGGAGCCC AACCTGGGGG AGGACGATGA GGACAAAGAC TTGGAGCCAG 
     2161 GCCCGTCGGG GACCTCGAAG GCCTCAGCGC AGATCTCCGG CCAGTCAGAC ACGGACATCA 
     2221 CAGCTGAGTT CCTGCAGCCT CTGCTGACGC CTGATAATGT GGCTAATCTG GTCCTCATCA 
     2281 GCATGGTGTA CCTACCCGAG GCCATGCCAG CCTCCTTCCA GGCCATCTAC ACCCCCGTGG 
     2341 AGTCAGCAGG CACGGAAGCC CAGATCAAGC ACCTGGCTCG GCTCATGGCC ACACAGATGA 
     2401 CAGCTGCCGG ACTGGGACCA GGTGTAGAGC AGACCAAACA GTGCAAGGAG GAGCCCAAGG 
     2461 AGGAGAAGGT GGTGAAGACA GAGAGCGTCC TGATCAAGCG GCGCCTGTCA GCCCAGGGCC 
     2521 AAGCCATCTC GGTGGTGGGT TCCCTGAGCT CCATGTCCCC CCTGGAGGAA GAGGCACCGC 
     2581 AGGCCAAGAG GAGGCCAGAG CCCATTATCC CTGTCACTCA GCCCCGGCTG GCAGGCGCTG 
     2641 GTGGGCGCAA GAAAATTTTC CGTCTCAGCG ACGTGCTGAA GCCCCTTACC GATGCCCAGG 
     2701 TGGAAGCCAT GAAGCTGGGC GCTGTGAAGC GGATCCTGCG GGCTGAGAAG GCTGTGGCCT 
     2761 GCAGCGGGGC AGCCCAGGTC CGCATAAAGA TCCTGGCCAG CCTGGTGACA CAGTTCAACT 
     2821 CGGGCCTGAA GGCGGAGGTC CTGTCCTTCA TCCTGGAGGA TGTGCGGGCC CGCCTGGACC 
     2881 TGGCCTTCGC CTGGCTCTAC CAGGAGTACA ACGCCTACCT GGCCGCAGGT GCCTCGGGCT 
     2941 CCCTGGACAA GTATGAGGAC TGCCTCATCC GCCTGTTGTC TGGCCTGCAG GAGAAACCAG 
     3001 ACCAGAAGGA TGGGATCTTC ACCAAGGTTG TGCTGGAGGC GCCACTCATC ACAGAGAGTG 
     3061 CCCTGGAGGT GGTCCGCAAG TACTGCGAGG ATGAGAGTCG CACCTATCTG GGCATGTCCA 
     3121 CACTTCGAGA CCTGATCTTC AAGCGCCCGT CCCGCCAGTT CCAGTACCTG CATGTCCTCC 
     3181 TCGACCTCAG CTCCCATGAG AAGGACAAGG TGCGCTCCCA GGCCCTGCTG TTCATCAAAC 
     3241 GCATGTATGA GAAGGAGCAG CTGCGGGAGT ATGTGGAGAA ATTTGCCCTC AACTACCTGC 
     3301 AGCTCCTGGT GCACCCCAAC CCACCGTCTG TGCTGTTTGG AGCTGACAAG GACACAGAGG 
     3361 TGGCAGCACC CTGGACGGAG GAGACAGTGA AGCAGTGTCT GTACCTCTAC CTGGCCCTCC 
     3421 TGCCTCAGAA CCACAAGCTG ATCCACGAAC TGGCGGCCGT GTACACTGAA GCCATCGCCG 
     3481 ACATCAAGCG GACGGTGCTG AGGGTCATTG AGCAGCCGAT CCGAGGAATG GGCATGAACT 
     3541 CCCCGGAGCT GCTCCTGCTG GTGGAAAATT GTCCCAAGGG AGCAGAGACA CTGGTCACGA 
     3601 GATGTCTGCA CAGCCTCACA GACAAAGTCC CACCCTCCCC AGAGCTGGTG AAGCGGGTCC 
     3661 GGGATCTCTA CCACAAGCGA CTGCCAGACG TCCGCTTCCT CATCCCGGTG CTCAATGGGC 
     3721 TGGAGAAGAA AGAGGTGATC CAGGCCCTGC CTAAACTCAT CAAACTCAAC CCCATCGTGG 
     3781 TGAAGGAAGT CTTCAACCGC CTGCTGGGCA CCCAGCATGG TGAGGGAAAC TCAGCCTTGT 
     3841 CCCCGCTGAA CCCTGGAGAG CTCCTGATCG CATTACACAA CATTGACTCC GTGAAGTGCG 
     3901 ACATGAAATC CATCATCAAA GCCACCAACC TGTGCTTTGC GGAGCGGAAC GTGTACACGT 
     3961 CAGAGGTGCT GGCCGTGGTG ATGCAGCAGC TGATGGAGCA GAGCCCCCTG CCCATGCTGC 
     4021 TCATGAGGAC CGTCATCCAG TCCCTGACCA TGTACCCCCG CCTGGGGGGC TTCGTCATGA 
     4081 ACATCCTGTC CCGCCTCATC ATGAAGCAGG TGTGGAAGTA CCCCAAGGTG TGGGAGGGCT 
     4141 TCATCAAGTG CTGCCAGCGC ACAAAGCCCC AGAGCTTCCA GGTCATCCTG CAGCTGCCGC 
     4201 CCCAGCAGCT GGGAGCCGTC TTTGACAAGT GCCCAGAGCT CCGGGAGCCC CTGCTGGCCC 
     4261 ATGTCCGCTC CTTCACCCCC CACCAGCAAG CTCACATCCC TAACTCCATC ATGACCATCT 
     4321 TGGAGGCCAG CGGCAAGCAG GAGCCAGAGG CCAAGGAGGC GCCTGCGGGG CCCTTGGAGG 
     4381 AGGATGATCT GGAGCCCCTG ACCTTGGCCC CGGCCCCAGC ACCCCGGCCC CCTCAGGACC 
     4441 TCATCGGCCT GCGACTGGCC CAGGAGAAGG CCTTAAAGCG GCAGCTGGAG GAGGAACAGA 
     4501 AGCTGAAGCC GGGAGGAGTG GGAGCCCCCT CCTCTTCCTC CCCCTCTCCC TCTCCGTCGG 
     4561 CCCGGCCAGG CCCGCCCCCG TCTGAGGAAG CCATGGATTT CCGGGAGGAG GGGCCTGAGT 
     4621 GCGAGACCCC GGGCATCTTC ATCAGCATGG ATGACGACTC GGGGCTGACC GAGGCCGCGC 
     4681 TGTTGGACTC TAGTCTCGAG GGCCCCCTAC CCAAGGAGAC GGCAGCGGGC GGGCTGACCT 
     4741 TGAAGGAGGA GCGGAGCCCC CAGACCCTCG CACCTGTTGG AGAAGATGCT ATGAAGACTC 
     4801 CCAGCCCGGC TGCCGAGGAC GCCAGGGAAC CCGAGGCCAA GGGGAACAGC ACGCGTACGC 
     4861 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     4921 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4981 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     5041 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     5101 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     5161 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     5221 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     5281 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     5341 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     5401 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     5461 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     5521 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     5581 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     5641 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     5701 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     5761 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     5821 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     5881 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     5941 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     6001 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     6061 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     6121 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     6181 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     6241 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     6301 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     6361 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     6421 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     6481 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     6541 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     6601 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     6661 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     6721 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     6781 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     6841 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     6901 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     6961 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     7021 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     7081 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     7141 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     7201 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     7261 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     7321 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     7381 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     7441 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     7501 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     7561 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     7621 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     7681 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     7741 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     7801 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     7861 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     7921 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     7981 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     8041 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     8101 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     8161 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     8221 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     8281 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     8341 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     8401 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     8461 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     8521 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     8581 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     8641 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     8701 TTT
//