LOCUS       RC216734                8889 bp    DNA     circular  SYN 
DEFINITION  SH3TC1 (Myc-DDK-tagged)-Human SH3 domain and tetratricopeptide repeats 1 (SH3TC1)
ACCESSION   NM_018986
VERSION     NM_018986.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5046..5137 
                     /label="Myc-DDK"  
     misc_feature    5167..5752 
                     /label="PolyA signal"  
     misc_feature    5224..5243 
                     /label="Restriction Enzyme"  
     misc_feature    6028..6700 
                     /label="ColE1"  
     misc_feature    7130..7924 
                     /label="Kan/Neo"  
     misc_feature    7959..8261 
                     /label="SV40 ori"  
     misc_feature    8347..8824 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5028..5034 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5028 
                     /label="SH3TC1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGAACCTC CCTGCCGTGA CCACTGAGGA GCCGACCCCC ATGGGGAGGG 
     1081 GTCCTGTGGG ACCCTCAGGA GGTGGCAGCA CCCGGGACCA GGTCCGGACT GTGGTCATGA 
     1141 GGCCCTCTGT GAGCTGGGAG AAAGCGGGGC CCGAGGAGGC CAAGGCGCCA GTGAGAGGCG 
     1201 ACGAGGCTCC TCCTGCCCGC GTGGCTGGGC CTGCTGCTGG GACCCCTCCC TGCCAGATGG 
     1261 GGGTTTATCC CACAGACCTG ACCCTGCAGC TGCTGGCTGT GCGGAGGAAG AGCAGACTGC 
     1321 GGGACCCCGG CCTACAGCAG ACCCTCCGGG GCCAGCTCCG CCTGCTGGAG AATGATAGCC 
     1381 GGGAGATGGC CCGCGTGCTT GGGGAATTAT CAGCCAGGCT GCTGTCCATC CACAGTGACC 
     1441 AGGACCGGAT CGTGGTGACG TTTAAGACTT TTGAAGAAAT CTGGAAGTTT TCCACCTACC 
     1501 ATGCTCTCGG CTTCACTCAT CACTGCCTGG CAAACCTGCT CATGGACCAG GCCTTCTGGC 
     1561 TGCTCTTGCC CAGTGAGGAG GAGGAGACGG CCATCCAAGT CCATGTGGAT GAGAACGCCT 
     1621 TAAGGCTGAC CCACGAGAGC CTCCTCATCC AAGAAGGGCC CTTCTTTGTC CTGTGTCCTG 
     1681 ACCACCATGT GAGAGTGATG ACGGGTCCCC GGGATGCAGG AAATGGCCCC CAGGCCCTCA 
     1741 GGCAGGCTTC GGGGGCACCC CAGGGAGAGG CGGCCCCGGA AACAGACTCT TCACCGCCGA 
     1801 GCCCCAGCGT GTCCTCCGAG GAGGTGGCAG TGGCGGCCGC CCCGGAGCCT TTGATTCCAT 
     1861 TTCATCAGTG GGCTCTTAGG ATCCCCCAGG ACCCCATCGA CGATGCCATG GGTGGCCCTG 
     1921 TGATGCCCGG CAACCCGCTG ATGGCTGTGG GCCTGGCCTC GGCATTGGCA GACTTCCAGG 
     1981 GCTCGGGGCC CGAAGAGATG ACCTTCCGAG GTGGCGACCT CATCGAGATC CTTGGGGCGC 
     2041 AGGTGCCCAG CCTGCCCTGG TGCGTGGGCC GACACGCAGC CTCGGGCCGG GTGGGGTTTG 
     2101 TGCGGAGCAG CCTCATCAGC ATGCAGGGCC CCGTGTCCGA GTTGGAAAGT GCGATTTTTC 
     2161 TCAATGAGGA AGAAAAGTCA TTCTTCAGCG AGGGCTGCTT TTCTGAGGAG GATGCCAGGC 
     2221 AGTTGCTGAG GCGGATGTCG GGCACCGATG TCTGCAGCGT GTACAGCCTG GACTCAGTAG 
     2281 AGGAAGCTGA GACCGAGCAG CCGCAGGAAA AAGAAATACC TCCACCTTGC CTGAGCCCGG 
     2341 AGCCACAGGA GACCTTGCAG AAGGTGAAGA ATGTTCTGGA ACAATGCAAG ACCTGCCCAG 
     2401 GCTGCCCCCA GGAGCCAGCG TCCTGGGGTC TCTGTGCGGC ATCCAGCGAC GTGAGCTTGC 
     2461 AGGACCCCGA GGAGCCCTCC TTCTGCTTGG AAGCCGAGGA CGACTGGGAG GACCCAGAGG 
     2521 CCCTGAGCTC ACTGCTGCTG TTCCTGAACG CCCCTGGGTA CAAGGCCAGC TTCCGTGGCC 
     2581 TGTACGATGT GGCGCTGCCG TGGCTGAGCA GCGTGTTCCG CAGCTTCAGC GACGAGGAGG 
     2641 AGCTGACTGG GCGCCTGGCA CAGGCCCGGG GGGCGGCCAA GAAAGCTGGC CTCCTCATGG 
     2701 CCCTGGCCAG GCTCTGCTTC CTCCTGGGGC GGCTGTGCAG CAGGAGGCTC AAGCTGTCCC 
     2761 AGGCCCGGGT GTACTTTGAG GAAGCGCTGG GGGCCCTGGA GGGCAGCTTC GGGGACCTGT 
     2821 TCCTAGTGGT GGCTGTGTAC GCCAACCTGG CCAGCATTTA CCGGAAGCAG AAGAACCGGG 
     2881 AGAAGTGTGC ACAGGTGGTG CCCAAAGCCA TGGCCCTGCT CCTGGGGACG CCTGACCACA 
     2941 TCTGCAGCAC CGAGGCGGAG GGGGAGCTCC TGCAGCTGGC GCTGCGGCGG GCGGTGGGTG 
     3001 GCCAGAGCCT GCAGGCCGAG GCCCGGGCCT GCTTCCTGCT GGCCAGGCAC CACGTGCACC 
     3061 TCAAGCAGCC CGAGGAGGCC CTGCCCTTCC TAGAGCGGCT GCTGCTTTTG CACAGGGACT 
     3121 CGGGAGCCCC AGAGGCCGCG TGGCTCTCAG ACTGCTACCT ACTCCTGGCT GACATCTACA 
     3181 GCCGCAAGTG CCTGCCCCAC CTGGTGCTGA GCTGTGTCAA GGTGGCCTCA TTGCGGACAC 
     3241 GGGGCTCGCT GGCCGGCTCG CTGAGGAGTG TGAACCTGGT GCTCCAGAAC GCCCCCCAGC 
     3301 CCCACAGCCT CCCTGCCCAA ACTTCCCACT ACCTCAGGCA AGCGCTGGCC TCCCTGACCC 
     3361 CGGGCACAGG CCAGGCGCTG CGCGGCCCCC TCTACACCAG CTTGGCCCAG CTGTACAGCC 
     3421 ACCATGGCTG CCACGGCCCG GCCATCACCT TCATGACGCA GGCAGTGGAA GCCAGTGCTA 
     3481 TTGCCGGAGT CCGTGCCATC GTGGACCACC TGGTGGCCCT GGCCTGGCTG CACGTGCTTC 
     3541 ATGGGCAGAG CCCGGTGGCC CTGGACATCC TGCAGTCTGT CCGGGATGCA GTGGTGGCCA 
     3601 GCGAGGACCA GGAGGGCGTG ATTGCCAACA TGGTGGCCGT GGCTCTGAAG AGGACGGGCC 
     3661 GGACGAGGCA GGCAGCTGAG AGCTACTACC GCGCCCTGCG GGTGGCTCGG GACCTGGGCC 
     3721 AGCAAAGGAA CCAGGCAGTG GGGCTGGCCA ACTTCGGGGC CCTGTGCCTG CATGCGGGTG 
     3781 CCAGCAGGCT GGCCCAGCAC TACCTCCTGG AGGCCGTGCG GCTGTTCTCG AGGCTGCCCC 
     3841 TTGGGGAGTG TGGCCGGGAC TTCACCCACG TGCTCCTGCA GCTGGGCCAT CTCTGCACCC 
     3901 GCCAGGGCCC GGCCCAGCAG GGCAAGGGCT ACTACGAGTG GGCCCTTCTG GTCGCCGTGG 
     3961 AGATGGGCCA CGTGGAGAGC CAGCTGCGGG CCGTCCAGCG GCTGTGCCAC TTCTACAGCG 
     4021 CCGTCATGCC CAGCGAGGCC CAGTGTGTCA TCTACCATGA GCTCCAGCTC TCCCTGGCCT 
     4081 GCAAGGTGGC CGACAAGGTG CTGGAGGGGC AGCTCCTGGA GACCATCAGC CAGCTCTACC 
     4141 TGTCCCTGGG CACCGAGCGG GCCTACAAAT CCGCACTGGA CTACACCAAA CGAAGTCTGG 
     4201 GGATTTTCAT TGACCTCCAG AAGAAAGAGA AGGAGGCGCA TGCCTGGCTG CAAGCAGGGA 
     4261 AGATCTATTA CATCTTGCGG CAGAGCGAGC TGGTGGACCT CTACATCCAG GTGGCACAGA 
     4321 ACGTGGCCCT GTACACAGGC GACCCCAACC TGGGGCTGGA GCTGTTTGAG GCGGCTGGAG 
     4381 ACATCTTCTT CGACGGGGCC TGGGAGCGGG AGAAAGCTGT GTCCTTCTAC CGGGACCGGG 
     4441 CCCTGCCCCT GGCAGTGACT ACGGGCAACC GCAAGGCGGA GCTGCGGCTG TGCAACAAGC 
     4501 TGGTGGCACT GCTGGCCACG CTGGAGGAGC CCCAGGAGGG CTTGGAGTTT GCCCACATGG 
     4561 CCCTAGCACT CAGCATCACC CTGGGGGACC GGCTGAACGA GCGCGTGGCC TACCACCGGC 
     4621 TGGCCGCCCT GCAACACCGA CTGGGCCATG GCGAGCTGGC AGAGCACTTC TACCTCAAGG 
     4681 CCCTGTCGCT CTGCAACTCG CCGCTGGAGT TTGACGAGGA GACCCTCTAC TACGTGAAGG 
     4741 TGTACCTGGT GCTCGGTGAC ATCATCTTCT ACGACCTGAA GGACCCGTTT GATGCAGCCG 
     4801 GGTACTACCA GCTGGCGCTG GCGGCCGCCG TGGACCTGGG CAACAAGAAG GCACAGCTGA 
     4861 AGATCTACAC GCGGCTGGCC ACCATCTACC ACAACTTCCT CCTGGACCGT GAGAAGTCGC 
     4921 TCTTCTTCTA CCAGAAGGCC AGGACCTTCG CCACAGAGCT CAACGTCCGC AGGGTCAACC 
     4981 TGCCTCCTCT GCCACTCTGC GGGTGGGCCC CCTGGTTGGC CCCCAGCCAC CCTCGCACGC 
     5041 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     5101 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     5161 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     5221 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     5281 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     5341 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     5401 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     5461 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     5521 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5581 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5641 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5701 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5761 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5821 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5881 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5941 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     6001 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     6061 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     6121 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     6181 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     6241 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     6301 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     6361 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     6421 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     6481 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6541 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6601 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6661 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6721 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6781 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6841 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6901 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6961 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     7021 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     7081 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     7141 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     7201 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     7261 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     7321 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     7381 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     7441 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     7501 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7561 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7621 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7681 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7741 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7801 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7861 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7921 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7981 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     8041 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     8101 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     8161 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     8221 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8281 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     8341 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     8401 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     8461 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     8521 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     8581 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8641 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8701 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8761 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8821 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8881 GCGAATTTT
//