LOCUS       RC216728                6702 bp    DNA     circular  SYN 
DEFINITION  ATG16L1 (Myc-DDK-tagged)-Human ATG16 autophagy related 16-like 1 (S. cerevisiae) (ATG16L1), transcript variant 1
ACCESSION   NM_030803
VERSION     NM_030803.6
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2859..2950 
                     /label="Myc-DDK"  
     misc_feature    2980..3565 
                     /label="PolyA signal"  
     misc_feature    3037..3056 
                     /label="Restriction Enzyme"  
     misc_feature    3841..4513 
                     /label="ColE1"  
     misc_feature    4943..5737 
                     /label="Kan/Neo"  
     misc_feature    5772..6074 
                     /label="SV40 ori"  
     misc_feature    6160..6637 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2841..2847 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2841 
                     /label="ATG16L1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCGTCGGGC CTCCGCGCCG CTGACTTCCC CCGCTGGAAG CGCCACATCT 
     1081 CGGAGCAACT GAGGCGCCGG GACCGGCTGC AGAGACAGGC GTTCGAGGAG ATCATCCTGC 
     1141 AGTATAACAA ATTGCTGGAA AAGTCAGATC TTCATTCAGT GTTGGCCCAG AAACTACAGG 
     1201 CTGAAAAGCA TGACGTACCA AACAGGCACG AGATAAGTCC CGGACATGAT GGCACATGGA 
     1261 ATGACAATCA GCTACAAGAA ATGGCCCAAC TGAGGATTAA GCACCAAGAG GAACTGACTG 
     1321 AATTACACAA GAAACGTGGG GAGTTAGCTC AACTGGTGAT TGACCTGAAT AACCAAATGC 
     1381 AGCGGAAGGA CAGGGAGATG CAGATGAATG AAGCAAAAAT TGCAGAATGT TTGCAGACTA 
     1441 TCTCTGACCT GGAGACGGAG TGCCTAGACC TGCGCACTAA GCTTTGTGAC CTTGAAAGAG 
     1501 CCAACCAGAC CCTGAAGGAT GAATATGATG CCCTGCAGAT CACTTTTACT GCCTTGGAGG 
     1561 GAAAACTGAG GAAAACTACG GAAGAGAACC AGGAGCTGGT CACCAGATGG ATGGCTGAGA 
     1621 AAGCCCAGGA AGCCAATCGG CTTAATGCAG AGAATGAAAA AGACTCCAGG AGGCGGCAAG 
     1681 CCCGGCTGCA GAAAGAGCTT GCAGAAGCAG CAAAGGAACC TCTACCAGTC GAACAGGATG 
     1741 ATGACATTGA GGTCATTGTG GATGAAACTT CTGATCACAC AGAAGAGACC TCTCCTGTGC 
     1801 GAGCCATCAG CAGAGCAGCC ACTAAGCGAC TCTCGCAGCC TGCTGGAGGC CTTCTGGATT 
     1861 CTATCACTAA TATCTTTGGG AGACGCTCTG TCTCTTCCTT CCCAGTCCCC CAGGACAATG 
     1921 TGGATACTCA TCCTGGTTCT GGTAAAGAAG TGAGGGTACC AGCTACTGCC TTGTGTGTCT 
     1981 TCGATGCACA TGATGGGGAA GTCAACGCTG TGCAGTTCAG TCCAGGTTCC CGGTTACTGG 
     2041 CCACTGGAGG CATGGACCGC AGGGTTAAGC TTTGGGAAGT ATTTGGAGAA AAATGTGAGT 
     2101 TCAAGGGTTC CCTATCTGGC AGTAATGCAG GAATTACAAG CATTGAATTT GATAGTGCTG 
     2161 GATCTTACCT CTTAGCAGCT TCAAATGATT TTGCAAGCCG AATCTGGACT GTGGATGATT 
     2221 ATCGATTACG GCACACACTC ACGGGACACA GTGGGAAAGT GCTGTCTGCT AAGTTCCTGC 
     2281 TGGACAATGC GCGGATTGTC TCAGGAAGTC ACGACCGGAC TCTCAAACTC TGGGATCTAC 
     2341 GCAGCAAAGT CTGCATAAAG ACAGTGTTTG CAGGATCCAG TTGCAATGAT ATTGTCTGCA 
     2401 CAGAGCAATG TGTAATGAGT GGACATTTTG ACAAGAAAAT TCGTTTCTGG GACATTCGAT 
     2461 CAGAGAGCAT AGTTCGAGAG ATGGAGCTGT TGGGAAAGAT TACTGCCCTG GACTTAAACC 
     2521 CAGAAAGGAC TGAGCTCCTG AGCTGCTCCC GTGATGACTT GCTAAAAGTT ATTGATCTCC 
     2581 GAACAAATGC TATCAAGCAG ACATTCAGTG CACCTGGGTT CAAGTGCGGC TCTGACTGGA 
     2641 CCAGAGTTGT CTTCAGCCCT GATGGCAGTT ACGTGGCGGC AGGCTCTGCT GAGGGCTCTC 
     2701 TGTATATCTG GAGTGTGCTC ACAGGGAAAG TGGAAAAGGT TCTTTCAAAG CAGCACAGCT 
     2761 CATCCATCAA TGCGGTGGCG TGGTCGCCCT CTGGCTCGCA CGTTGTCAGT GTGGACAAAG 
     2821 GATGCAAAGC TGTGCTGTGG GCACAGTACA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     2881 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     2941 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     3001 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     3061 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     3121 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     3181 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     3241 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     3301 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     3361 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     3421 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     3481 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     3541 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     3601 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     3661 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     3721 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     3781 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     3841 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     3901 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     3961 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     4021 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     4081 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     4141 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     4201 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     4261 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     4321 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     4381 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     4441 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     4501 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     4561 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     4621 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     4681 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     4741 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     4801 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     4861 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     4921 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     4981 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     5041 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     5101 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     5161 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     5221 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     5281 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     5341 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     5401 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     5461 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     5521 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     5581 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     5641 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     5701 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     5761 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     5821 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     5881 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     5941 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     6001 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     6061 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     6121 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     6181 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     6241 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     6301 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     6361 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     6421 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     6481 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     6541 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     6601 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     6661 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//