LOCUS       RC216727L1                9000 bp    DNA     circular  SYN 
DEFINITION  UBE3A (Myc-DDK-tagged)-Human ubiquitin protein ligase E3A (UBE3A), transcript variant 2
ACCESSION   NM_000462
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7291..7471 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    8199..8858 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    7630..8198 
                     /label="ColE1"  
     misc_feature    6166..6186 
                     /label="LR50 primer"  
     misc_feature    5963..6034 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    6175..6766 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5945..5951 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5945 
                     /label="UBE3A"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGAGAAGCTG CACCAGTGTT ATTGGAAATC 
     3361 AGGAGAACCT CAGTCTGACG ACATTGAAGC TAGCCGAATG AAGCGAGCAG CTGCAAAGCA 
     3421 TCTAATAGAA CGCTACTACC ACCAGTTAAC TGAGGGCTGT GGAAATGAAG CCTGCACGAA 
     3481 TGAGTTTTGT GCTTCCTGTC CAACTTTTCT TCGTATGGAT AATAATGCAG CAGCTATTAA 
     3541 AGCCCTCGAG CTTTATAAGA TTAATGCAAA ACTCTGTGAT CCTCATCCCT CCAAGAAAGG 
     3601 AGCAAGCTCA GCTTACCTTG AGAACTCGAA AGGTGCCCCC AACAACTCCT GCTCTGAGAT 
     3661 AAAAATGAAC AAGAAAGGCG CTAGAATTGA TTTTAAAGAT GTGACTTACT TAACAGAAGA 
     3721 GAAGGTATAT GAAATTCTTG AATTATGTAG AGAAAGAGAG GATTATTCCC CTTTAATCCG 
     3781 TGTTATTGGA AGAGTTTTTT CTAGTGCTGA GGCATTGGTA CAGAGCTTCC GGAAAGTTAA 
     3841 ACAACACACC AAGGAAGAAC TGAAATCTCT TCAAGCAAAA GATGAAGACA AAGATGAAGA 
     3901 TGAAAAGGAA AAAGCTGCAT GTTCTGCTGC TGCTATGGAA GAAGACTCAG AAGCATCTTC 
     3961 CTCAAGGATA GGTGATAGCT CACAGGGAGA CAACAATTTG CAAAAATTAG GCCCTGATGA 
     4021 TGTGTCTGTG GATATTGATG CCATTAGAAG GGTCTACACC AGATTGCTCT CTAATGAAAA 
     4081 AATTGAAACT GCCTTTCTCA ATGCACTTGT ATATTTGTCA CCTAACGTGG AATGTGACTT 
     4141 GACGTATCAC AATGTATACT CTCGAGATCC TAATTATCTG AATTTGTTCA TTATCGTAAT 
     4201 GGAGAATAGA AATCTCCACA GTCCTGAATA TCTGGAAATG GCTTTGCCAT TATTTTGCAA 
     4261 AGCGATGAGC AAGCTACCCC TTGCAGCCCA AGGAAAACTG ATCAGACTGT GGTCTAAATA 
     4321 CAATGCAGAC CAGATTCGGA GAATGATGGA GACATTTCAG CAACTTATTA CTTATAAAGT 
     4381 CATAAGCAAT GAATTTAACA GTCGAAATCT AGTGAATGAT GATGATGCCA TTGTTGCTGC 
     4441 TTCGAAGTGC TTGAAAATGG TTTACTATGC AAATGTAGTG GGAGGGGAAG TGGACACAAA 
     4501 TCACAATGAA GAAGATGATG AAGAGCCCAT CCCTGAGTCC AGCGAGCTGA CACTTCAGGA 
     4561 ACTTTTGGGA GAAGAAAGAA GAAACAAGAA AGGTCCTCGA GTGGACCCCC TGGAAACTGA 
     4621 ACTTGGTGTT AAAACCCTGG ATTGTCGAAA ACCACTTATC CCTTTTGAAG AGTTTATTAA 
     4681 TGAACCACTG AATGAGGTTC TAGAAATGGA TAAAGATTAT ACTTTTTTCA AAGTAGAAAC 
     4741 AGAGAACAAA TTCTCTTTTA TGACATGTCC CTTTATATTG AATGCTGTCA CAAAGAATTT 
     4801 GGGATTATAT TATGACAATA GAATTCGCAT GTACAGTGAA CGAAGAATCA CTGTTCTCTA 
     4861 CAGCTTAGTT CAAGGACAGC AGTTGAATCC ATATTTGAGA CTCAAAGTTA GACGTGACCA 
     4921 TATCATAGAT GATGCACTTG TCCGGCTAGA GATGATCGCT ATGGAAAATC CTGCAGACTT 
     4981 GAAGAAGCAG TTGTATGTGG AATTTGAAGG AGAACAAGGA GTTGATGAGG GAGGTGTTTC 
     5041 CAAAGAATTT TTTCAGCTGG TTGTGGAGGA AATCTTCAAT CCAGATATTG GTATGTTCAC 
     5101 ATACGATGAA TCTACAAAAT TGTTTTGGTT TAATCCATCT TCTTTTGAAA CTGAGGGTCA 
     5161 GTTTACTCTG ATTGGCATAG TACTGGGTCT GGCTATTTAC AATAACTGTA TACTGGATGT 
     5221 ACATTTTCCC ATGGTTGTCT ACAGGAAGCT AATGGGGAAA AAAGGAACTT TTCGTGACTT 
     5281 GGGAGACTCT CACCCAGTTC TATATCAGAG TTTAAAAGAT TTATTGGAGT ATGAAGGGAA 
     5341 TGTGGAAGAT GACATGATGA TCACTTTCCA GATATCACAG ACAGATCTTT TTGGTAACCC 
     5401 AATGATGTAT GATCTAAAGG AAAATGGTGA TAAAATTCCA ATTACAAATG AAAACAGGAA 
     5461 GGAATTTGTC AATCTTTATT CTGACTACAT TCTCAATAAA TCAGTAGAAA AACAGTTCAA 
     5521 GGCTTTTCGG AGAGGTTTTC ATATGGTGAC CAATGAATCT CCCTTAAAGT ACTTATTCAG 
     5581 ACCAGAAGAA ATTGAATTGC TTATATGTGG AAGCCGGAAT CTAGATTTCC AAGCACTAGA 
     5641 AGAAACTACA GAATATGACG GTGGCTATAC CAGGGACTCT GTTCTGATTA GGGAGTTCTG 
     5701 GGAAATCGTT CATTCATTTA CAGATGAACA GAAAAGACTC TTCTTGCAGT TTACAACGGG 
     5761 CACAGACAGA GCACCTGTGG GAGGACTAGG AAAATTAAAG ATGATTATAG CCAAAAATGG 
     5821 CCCAGACACA GAAAGGTTAC CTACATCTCA TACTTGCTTT AATGTGCTTT TACTTCCGGA 
     5881 ATACTCAAGC AAAGAAAAAC TTAAAGAGAG ATTGTTGAAG GCCATCACGT ATGCCAAAGG 
     5941 ATTTGGCATG CTGACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT 
     6001 GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG 
     6061 CGGTCTGTAC AAGTAGGATT CGTCGAGGGA CCTAATAACT TCGTATAGCA TACATTATAC 
     6121 GAAGTTATAC ATGTTTAAGG GTTCCGGTTC CACTAGGTAC AATTCGATAT CAAGCTTATC 
     6181 GATAATCAAC CTCTGGATTA CAAAATTTGT GAAAGATTGA CTGGTATTCT TAACTATGTT 
     6241 GCTCCTTTTA CGCTATGTGG ATACGCTGCT TTAATGCCTT TGTATCATGC TATTGCTTCC 
     6301 CGTATGGCTT TCATTTTCTC CTCCTTGTAT AAATCCTGGT TGCTGTCTCT TTATGAGGAG 
     6361 TTGTGGCCCG TTGTCAGGCA ACGTGGCGTG GTGTGCACTG TGTTTGCTGA CGCAACCCCC 
     6421 ACTGGTTGGG GCATTGCCAC CACCTGTCAG CTCCTTTCCG GGACTTTCGC TTTCCCCCTC 
     6481 CCTATTGCCA CGGCGGAACT CATCGCCGCC TGCCTTGCCC GCTGCTGGAC AGGGGCTCGG 
     6541 CTGTTGGGCA CTGACAATTC CGTGGTGTTG TCGGGGAAAT CATCGTCCTT TCCTTGGCTG 
     6601 CTCGCCTGTG TTGCCACCTG GATTCTGCGC GGGACGTCCT TCTGCTACGT CCCTTCGGCC 
     6661 CTCAATCCAG CGGACCTTCC TTCCCGCGGC CTGCTGCCGG CTCTGCGGCC TCTTCCGCGT 
     6721 CTTCGCCTTC GCCCTCAGAC GAGTCGGATC TCCCTTTGGG CCGCCTCCCC GCATCGATAC 
     6781 CGTCGACCTC GATCGAGACC TAGAAAAACA TGGAGCAATC ACAAGTAGCA ATACAGCAGC 
     6841 TACCAATGCT GATTGTGCCT GGCTAGAAGC ACAAGAGGAG GAGGAGGTGG GTTTTCCAGT 
     6901 CACACCTCAG GTACCTTTAA GACCAATGAC TTACAAGGCA GCTGTAGATC TTAGCCACTT 
     6961 TTTAAAAGAA AAGGGGGGAC TGGAAGGGCT AATTCACTCC CAACGAAGAC AAGATATCCT 
     7021 TGATCTGTGG ATCTACCACA CACAAGGCTA CTTCCCTGAT TGGCAGAACT ACACACCAGG 
     7081 GCCAGGGATC AGATATCCAC TGACCTTTGG ATGGTGCTAC AAGCTAGTAC CAGTTGAGCA 
     7141 AGAGAAGGTA GAAGAAGCCA ATGAAGGAGA GAACACCCGC TTGTTACACC CTGTGAGCCT 
     7201 GCATGGGATG GATGACCCGG AGAGAGAAGT ATTAGAGTGG AGGTTTGACA GCCGCCTAGC 
     7261 ATTTCATCAC ATGGCCCGAG AGCTGCATCC GGACTGTACT GGGTCTCTCT GGTTAGACCA 
     7321 GATCTGAGCC TGGGAGCTCT CTGGCTAACT AGGGAACCCA CTGCTTAAGC CTCAATAAAG 
     7381 CTTGCCTTGA GTGCTTCAAG TAGTGTGTGC CCGTCTGTTG TGTGACTCTG GTAACTAGAG 
     7441 ATCCCTCAGA CCCTTTTAGT CAGTGTGGAA AATCTCTAGC AGCATGTGAG CAAAAGGCCA 
     7501 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     7561 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     7621 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     7681 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     7741 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     7801 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     7861 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     7921 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     7981 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     8041 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     8101 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     8161 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGATTA CGCCCCGCCC 
     8221 TGCCACTCAT CGCAGTACTG TTGTAATTCA TTAAGCATTC TGCCGACATG GAAGCCATCA 
     8281 CAAACGGCAT GATGAACCTG AATCGCCAGC GGCATCAGCA CCTTGTCGCC TTGCGTATAA 
     8341 TATTTGCCCA TGGTGAAAAC GGGGGCGAAG AAGTTGTCCA TATTGGCCAC GTTTAAATCA 
     8401 AAACTGGTGA AACTCACCCA GGGATTGGCT GAGACGAAAA ACATATTCTC AATAAACCCT 
     8461 TTAGGGAAAT AGGCCAGGTT TTCACCGTAA CACGCCACAT CTTGCGAATA TATGTGTAGA 
     8521 AACTGCCGGA AATCGTCGTG GTATTCACTC CAGAGCGATG AAAACGTTTC AGTTTGCTCA 
     8581 TGGAAAACGG TGTAACAAGG GTGAACACTA TCCCATATCA CCAGCTCACC GTCTTTCATT 
     8641 GCCATACGGA ACTCCGGATG AGCATTCATC AGGCGGGCAA GAATGTGAAT AAAGGCCGGA 
     8701 TAAAACTTGT GCTTATTTTT CTTTACGGTC TTTAAAAAGG CCGTAATATC CAGCTGAACG 
     8761 GTCTGGTTAT AGGTACATTG AGCAACTGAC TGAAATGCCT CAAAATGTTC TTTACGATGC 
     8821 CATTGGGATA TATCAACGGT GGTATATCCA GTGATTTTTT TCTCCATACT CTTCCTTTTT 
     8881 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     8941 ATTTAGAAAA ATAAACAAAT AGGGGTCCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
//