LOCUS       RC216722                8445 bp    DNA     circular  SYN 
DEFINITION  ITGA11 (Myc-DDK-tagged)-Human integrin, alpha 11 (ITGA11)
ACCESSION   NM_001004439
VERSION     NM_001004439.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4602..4693 
                     /label="Myc-DDK"  
     misc_feature    4723..5308 
                     /label="PolyA signal"  
     misc_feature    4780..4799 
                     /label="Restriction Enzyme"  
     misc_feature    5584..6256 
                     /label="ColE1"  
     misc_feature    6686..7480 
                     /label="Kan/Neo"  
     misc_feature    7515..7817 
                     /label="SV40 ori"  
     misc_feature    7903..8380 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4584..4590 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4584 
                     /label="ITGA11"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGACCTGCCC AGGGGCCTGG TGGTGGCCTG GGCGCTCAGC CTGTGGCCAG 
     1081 GGTTCACGGA CACCTTCAAC ATGGACACCA GGAAGCCCCG GGTCATCCCT GGCTCCAGGA 
     1141 CCGCCTTCTT TGGCTACACA GTGCAGCAGC ACGACATCAG TGGCAATAAG TGGCTGGTCG 
     1201 TGGGCGCCCC ACTGGAAACC AATGGCTACC AGAAGACGGG AGACGTGTAC AAGTGTCCAG 
     1261 TGATCCACGG GAACTGCACC AAACTCAACC TGGGAAGGGT CACCCTGTCC AACGTGTCCG 
     1321 AGCGGAAAGA CAACATGCGC CTCGGCCTTA GTCTCGCCAC CAACCCCAAG GACAACAGCT 
     1381 TCCTGGCCTG CAGCCCCCTC TGGTCTCATG AGTGTGGGAG CTCCTACTAC ACCACAGGGA 
     1441 TGTGTTCAAG AGTCAACTCC AACTTCAGGT TCTCCAAGAC CGTGGCCCCA GCTCTCCAAA 
     1501 GGTGCCAGAC CTACATGGAC ATCGTCATTG TCCTGGATGG CTCCAACAGC ATCTACCCCT 
     1561 GGGTGGAGGT TCAGCACTTC CTCATCAACA TCCTGAAAAA GTTTTACATT GGCCCAGGGC 
     1621 AGATCCAGGT TGGAGTTGTG CAGTATGGCG AAGATGTGGT GCATGAGTTT CACCTCAACG 
     1681 ACTACAGGTC TGTAAAAGAT GTGGTGGAAG CTGCCAGCCA CATTGAGCAG AGAGGAGGAA 
     1741 CAGAGACCCG GACGGCATTT GGCATTGAAT TTGCACGCTC AGAGGCTTTC CAGAAGGGTG 
     1801 GAAGGAAAGG AGCCAAGAAG GTGATGATTG TCATCACAGA TGGGGAGTCC CACGACAGCC 
     1861 CAGACCTGGA GAAGGTGATC CAGCAAAGCG AAAGAGACAA CGTAACAAGA TATGCGGTGG 
     1921 CCGTCCTGGG CTACTACAAC CGCAGGGGGA TCAATCCAGA AACTTTTCTA AATGAAATCA 
     1981 AATACATCGC CAGTGACCCT GATGACAAGC ACTTCTTCAA TGTCACTGAT GAGGCTGCCT 
     2041 TGAAGGACAT TGTCGATGCC CTGGGGGACA GAATCTTCAG CCTGGAAGGC ACCAACAAGA 
     2101 ACGAGACCTC CTTTGGGCTG GAGATGTCAC AGACGGGCTT TTCCTCGCAC GTGGTGGAGG 
     2161 ATGGGGTTCT GCTGGGAGCC GTCGGTGCCT ATGACTGGAA TGGAGCTGTG CTAAAGGAGA 
     2221 CGAGTGCCGG GAAGGTCATT CCTCTCCGCG AGTCCTACCT GAAAGAGTTC CCCGAGGAGC 
     2281 TCAAGAACCA TGGTGCATAC CTGGGGTACA CAGTCACATC GGTCGTGTCC TCCAGGCAGG 
     2341 GGCGGGTGTA CGTGGCCGGA GCCCCCCGGT TCAACCACAC GGGCAAGGTC ATCCTGTTCA 
     2401 CCATGCACAA CAACCGGAGC CTCACCATCC ACCAGGCTAT GCGGGGCCAG CAGATAGGCT 
     2461 CTTACTTTGG GAGTGAAATC ACCTCGGTGG ACATCGACGG CGACGGCGTG ACTGATGTCC 
     2521 TGCTGGTGGG CGCACCCATG TACTTCAACG AGGGCCGTGA GCGAGGCAAG GTGTACGTCT 
     2581 ATGAGCTGAG ACAGAACCTG TTTGTTTATA ACGGAACGCT AAAGGATTCA CACAGTTACC 
     2641 AGAATGCCCG ATTTGGGTCC TCCATTGCCT CAGTTCGAGA CCTCAACCAG GATTCCTACA 
     2701 ATGACGTGGT GGTGGGAGCC CCCCTGGAGG ACAACCACGC AGGAGCCATC TACATCTTCC 
     2761 ACGGCTTCCG AGGCAGCATC CTGAAGACAC CTAAGCAGAG AATCACAGCC TCAGAGCTGG 
     2821 CTACCGGCCT CCAGTATTTT GGCTGCAGCA TCCACGGGCA ATTGGACCTC AATGAGGATG 
     2881 GGCTCATCGA CCTGGCAGTG GGAGCCCTTG GCAACGCTGT GATTCTGTGG TCCCGCCCAG 
     2941 TGGTTCAGAT CAATGCCAGC CTCCACTTTG AGCCATCCAA GATCAACATC TTCCACAGAG 
     3001 ACTGCAAGCG CAGTGGCAGG GATGCCACCT GCCTGGCCGC CTTCCTCTGC TTCACGCCCA 
     3061 TCTTCCTGGC ACCCCATTTC CAAACAACAA CTGTTGGCAT CAGATACAAC GCCACCATGG 
     3121 ATGAGAGGCG GTATACACCG AGGGCCCACC TGGACGAGGG CGGGGACCGA TTCACCAACA 
     3181 GAGCCGTACT GCTCTCCTCC GGCCAGGAGC TCTGTGAGCG GATCAACTTC CATGTCCTGG 
     3241 ACACTGCTGA CTACGTGAAG CCAGTGACCT TCTCAGTCGA GTATTCCCTG GAGGACCCTG 
     3301 ACCATGGCCC CATGCTGGAC GACGGCTGGC CCACCACTCT CAGAGTCTCG GTGCCCTTCT 
     3361 GGAACGGCTG CAATGAGGAT GAGCACTGTG TCCCTGACCT TGTGTTGGAT GCCCGGAGTG 
     3421 ACCTGCCCAC GGCCATGGAG TACTGCCAGA GGGTGCTGAG GAAGCCTGCG CAGGACTGCT 
     3481 CCGCATACAC GCTGTCCTTC GACACCACAG TCTTCATCAT AGAGAGCACA CGCCAGCGAG 
     3541 TGGCGGTGGA GGCCACACTG GAGAACAGGG GCGAGAACGC CTACAGCACG GTCCTAAATA 
     3601 TCTCGCAGTC AGCAAACCTG CAGTTTGCCA GCTTGATCCA GAAGGAGGAC TCAGACGGTA 
     3661 GCATTGAGTG TGTGAACGAG GAGAGGAGGC TCCAGAAGCA AGTCTGCAAC GTCAGCTATC 
     3721 CCTTCTTCCG GGCCAAGGCC AAGGTGGCTT TCCGTCTTGA TTTTGAGTTC AGCAAATCCA 
     3781 TCTTCCTACA CCACCTGGAG ATCGAGCTCG CTGCAGGCAG TGACAGTAAT GAGCGGGACA 
     3841 GCACCAAGGA AGACAACGTG GCCCCCTTAC GCTTCCACCT CAAATACGAG GCTGACGTCC 
     3901 TCTTCACCAG GAGCAGCAGC CTGAGCCACT ACGAGGTCAA GCCCAACAGC TCGCTGGAGA 
     3961 GATACGATGG TATCGGGCCT CCCTTCAGCT GCATCTTCAG GATCCAGAAC TTGGGCTTGT 
     4021 TCCCCATCCA CGGGATGATG ATGAAGATCA CCATTCCCAT CGCCACCAGG AGCGGCAACC 
     4081 GCCTACTGAA GCTGAGGGAC TTCCTCACGG ACGAGGCGAA CACGTCCTGT AACATCTGGG 
     4141 GCAATAGCAC TGAGTACCGG CCCACCCCAG TGGAGGAAGA CTTGCGTCGT GCTCCACAGC 
     4201 TGAATCACAG CAACTCTGAT GTCGTCTCCA TCAACTGCAA TATACGGCTG GTCCCCAACC 
     4261 AGGAAATCAA TTTCCATCTA CTGGGGAACC TGTGGTTGAG GTCCCTAAAA GCACTCAAGT 
     4321 ACAAATCCAT GAAAATCATG GTCAACGCAG CCTTGCAGAG GCAGTTCCAC AGCCCCTTCA 
     4381 TCTTCCGTGA GGAGGATCCC AGCCGCCAGA TCGTGTTTGA GATCTCCAAG CAAGAGGACT 
     4441 GGCAGGTCCC CATCTGGATC ATTGTAGGCA GCACCCTGGG GGGCCTCCTA CTGCTGGCCC 
     4501 TGCTGGTCCT GGCACTGTGG AAGCTCGGCT TCTTTAGAAG TGCCAGGCGC AGGAGGGAGC 
     4561 CTGGTCTGGA CCCCACCCCC AAAGTGCTGG AGACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     4621 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     4681 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4741 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4801 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     4861 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     4921 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     4981 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     5041 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     5101 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     5161 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5221 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5281 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5341 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     5401 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     5461 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     5521 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     5581 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     5641 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     5701 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     5761 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     5821 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     5881 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     5941 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     6001 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     6061 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     6121 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     6181 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     6241 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     6301 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     6361 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     6421 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     6481 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     6541 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     6601 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     6661 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     6721 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     6781 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     6841 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     6901 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     6961 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     7021 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     7081 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     7141 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     7201 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     7261 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     7321 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     7381 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     7441 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     7501 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     7561 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     7621 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     7681 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7741 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7801 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     7861 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     7921 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     7981 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     8041 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     8101 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     8161 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     8221 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     8281 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     8341 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     8401 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//