LOCUS RC216521 7626 bp DNA circular SYN DEFINITION ENPP2 (Myc-DDK-tagged)-Human ectonucleotide pyrophosphatase/phosphodiesterase 2 (ENPP2), transcript variant 1 ACCESSION NM_006209 VERSION null SOURCE synthetic construct FEATURES Location/Qualifiers misc_feature 201..924 /label="CMV promoter" misc_feature 839..856 /label="Restriction Enzyme" misc_feature 953..971 /label="T7 promoter" misc_feature 3783..3874 /label="Myc-DDK" misc_feature 3904..4489 /label="PolyA signal" misc_feature 3961..3980 /label="Restriction Enzyme" misc_feature 4765..5437 /label="ColE1" misc_feature 5867..6661 /label="Kan/Neo" misc_feature 6696..6998 /label="SV40 ori" misc_feature 7084..7561 /label="f1 ori" misc_feature 1020..1028 /label="Restriction Enzyme" misc_feature 3765..3771 /label="Restriction Enzyme" misc_feature 1020..3765 /label="ENPP2" ORIGIN 1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 1021 CGATCGCCAT GGCAAGGAGG AGCTCGTTCC AGTCGTGTCA GATAATATCC CTGTTCACTT 1081 TTGCCGTTGG AGTCAATATC TGCTTAGGAT TCACTGCACA TCGAATTAAG AGAGCAGAAG 1141 GATGGGAGGA AGGTCCTCCT ACAGTGCTAT CAGACTCCCC CTGGACCAAC ATCTCCGGAT 1201 CTTGCAAGGG CAGGTGCTTT GAACTTCAAG AGGCTGGACC TCCTGATTGT CGCTGTGACA 1261 ACTTGTGTAA GAGCTATACC AGTTGCTGCC ATGACTTTGA TGAGCTGTGT TTGAAGACAG 1321 CCCGTGGCTG GGAGTGTACT AAGGACAGAT GTGGAGAAGT CAGAAATGAA GAAAATGCCT 1381 GTCACTGCTC AGAGGACTGC TTGGCCAGGG GAGACTGCTG TACCAATTAC CAAGTGGTTT 1441 GCAAAGGAGA GTCGCATTGG GTTGATGATG ACTGTGAGGA AATAAAGGCC GCAGAATGCC 1501 CTGCAGGGTT TGTTCGCCCT CCATTAATCA TCTTCTCCGT GGATGGCTTC CGTGCATCAT 1561 ACATGAAGAA AGGCAGCAAA GTCATGCCTA ATATTGAAAA ACTAAGGTCT TGTGGCACAC 1621 ACTCTCCCTA CATGAGGCCG GTGTACCCAA CTAAAACCTT TCCTAACTTA TACACTTTGG 1681 CCACTGGGCT ATATCCAGAA TCACATGGAA TTGTTGGCAA TTCAATGTAT GATCCTGTAT 1741 TTGATGCCAC TTTTCATCTG CGAGGGCGAG AGAAATTTAA TCATAGATGG TGGGGAGGTC 1801 AACCGCTATG GATTACAGCC ACCAAGCAAG GGGTGAAAGC TGGAACATTC TTTTGGTCTG 1861 TTGTCATCCC TCACGAGCGG AGAATATTAA CCATATTGCA GTGGCTCACC CTGCCAGATC 1921 ATGAGAGGCC TTCGGTCTAT GCCTTCTATT CTGAGCAACC TGATTTCTCT GGACACAAAT 1981 ATGGCCCTTT CGGCCCTGAG GAGAGTAGTT ATGGCTCACC TTTTACTCCG GCTAAGAGAC 2041 CTAAGAGGAA AGTTGCCCCT AAGAGGAGAC AGGAAAGACC AGTTGCTCCT CCAAAGAAAA 2101 GAAGAAGAAA AATACATAGG ATGGATCATT ATGCTGCGGA AACTCGTCAG GACAAAATGA 2161 CAAATCCTCT GAGGGAAATC GACAAAATTG TGGGGCAATT AATGGATGGA CTGAAACAAC 2221 TAAAACTGCA TCGGTGTGTC AACGTCATCT TTGTCGGAGA CCATGGAATG GAAGATGTCA 2281 CATGTGATAG AACTGAGTTC TTGAGTAATT ACCTAACTAA TGTGGATGAT ATTACTTTAG 2341 TGCCTGGAAC TCTAGGAAGA ATTCGATCCA AATTTAGCAA CAATGCTAAA TATGACCCCA 2401 AAGCCATTAT TGCCAATCTC ACGTGTAAAA AACCAGATCA GCACTTTAAG CCTTACTTGA 2461 AACAGCACCT TCCCAAACGT TTGCACTATG CCAACAACAG AAGAATTGAG GATATCCATT 2521 TATTGGTGGA ACGCAGATGG CATGTTGCAA GGAAACCTTT GGATGTTTAT AAGAAACCAT 2581 CAGGAAAATG CTTTTTCCAG GGAGACCACG GATTTGATAA CAAGGTCAAC AGCATGCAGA 2641 CTGTTTTTGT AGGTTATGGC CCAACATTTA AGTACAAGAC TAAAGTGCCT CCATTTGAAA 2701 ACATTGAACT TTACAATGTT ATGTGTGATC TCCTGGGATT GAAGCCAGCT CCTAATAATG 2761 GGACCCATGG AAGTTTGAAT CATCTCCTGC GCACTAATAC CTTCAGGCCA ACCATGCCAG 2821 AGGAAGTTAC CAGACCCAAT TATCCAGGGA TTATGTACCT TCAGTCTGAT TTTGACCTGG 2881 GCTGCACTTG TGATGATAAG GTAGAGCCAA AGAACAAGTT GGATGAACTC AACAAACGGC 2941 TTCATACAAA AGGGTCTACA GAAGAGAGAC ACCTCCTCTA TGGGCGACCT GCAGTGCTTT 3001 ATCGGACTAG ATATGATATC TTATATCACA CTGACTTTGA AAGTGGTTAT AGTGAAATAT 3061 TCCTAATGCC ACTCTGGACA TCATATACTG TTTCCAAACA GGCTGAGGTT TCCAGCGTTC 3121 CTGACCATCT GACCAGTTGC GTCCGGCCTG ATGTCCGTGT TTCTCCGAGT TTCAGTCAGA 3181 ACTGTTTGGC CTACAAAAAT GATAAGCAGA TGTCCTACGG ATTCCTCTTT CCTCCTTATC 3241 TGAGCTCTTC ACCAGAGGCT AAATATGATG CATTCCTTGT AACCAATATG GTTCCAATGT 3301 ATCCTGCTTT CAAACGGGTC TGGAATTATT TCCAAAGGGT ATTGGTGAAG AAATATGCTT 3361 CGGAAAGAAA TGGAGTTAAC GTGATAAGTG GACCAATCTT CGACTATGAC TATGATGGCT 3421 TACATGACAC AGAAGACAAA ATAAAACAGT ACGTGGAAGG CAGTTCCATT CCTGTTCCAA 3481 CTCACTACTA CAGCATCATC ACCAGCTGTC TGGATTTCAC TCAGCCTGCC GACAAGTGTG 3541 ACGGCCCTCT CTCTGTGTCC TCCTTCATCC TGCCTCACCG GCCTGACAAC GAGGAGAGCT 3601 GCAATAGCTC AGAGGACGAA TCAAAATGGG TAGAAGAACT CATGAAGATG CACACAGCTA 3661 GGGTGCGTGA CATTGAACAT CTCACCAGCC TGGACTTCTT CCGAAAGACC AGCCGCAGCT 3721 ACCCAGAAAT CCTGACACTC AAGACATACC TGCATACATA TGAGAGCGAG ATTACGCGTA 3781 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 3841 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 3901 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 3961 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 4021 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 4081 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 4141 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 4201 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 4261 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 4321 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 4381 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 4441 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 4501 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 4561 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 4621 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 4681 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 4741 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 4801 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 4861 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 4921 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 4981 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 5041 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 5101 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 5161 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 5221 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 5281 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 5341 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 5401 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 5461 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 5521 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 5581 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 5641 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 5701 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 5761 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 5821 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 5881 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 5941 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 6001 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 6061 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 6121 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 6181 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 6241 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 6301 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 6361 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 6421 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 6481 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 6541 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 6601 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 6661 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 6721 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 6781 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 6841 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 6901 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 6961 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 7021 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 7081 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 7141 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 7201 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 7261 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 7321 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 7381 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 7441 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 7501 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 7561 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 7621 AATTTT //