LOCUS       RC216503                8010 bp    DNA     circular  SYN 
DEFINITION  RAG1 (Myc-DDK-tagged)-Human recombination activating gene 1 (RAG1)
ACCESSION   NM_000448
VERSION     NM_000448.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4167..4258 
                     /label="Myc-DDK"  
     misc_feature    4288..4873 
                     /label="PolyA signal"  
     misc_feature    4345..4364 
                     /label="Restriction Enzyme"  
     misc_feature    5149..5821 
                     /label="ColE1"  
     misc_feature    6251..7045 
                     /label="Kan/Neo"  
     misc_feature    7080..7382 
                     /label="SV40 ori"  
     misc_feature    7468..7945 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4149..4155 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4149 
                     /label="RAG1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCAGCCTCT TTCCCACCCA CCTTGGGACT CAGTTCTGCC CCAGATGAAA 
     1081 TTCAGCACCC ACATATTAAA TTTTCAGAAT GGAAATTTAA GCTGTTCCGG GTGAGATCCT 
     1141 TTGAAAAGAC ACCTGAAGAA GCTCAAAAGG AAAAGAAGGA TTCCTTTGAG GGGAAACCCT 
     1201 CTCTGGAGCA ATCTCCAGCA GTCCTGGACA AGGCTGATGG TCAGAAGCCA GTCCCAACTC 
     1261 AGCCATTGTT AAAAGCCCAC CCTAAGTTTT CAAAGAAATT TCACGACAAC GAGAAAGCAA 
     1321 GAGGCAAAGC GATCCATCAA GCCAACCTTC GACATCTCTG CCGCATCTGT GGGAATTCTT 
     1381 TTAGAGCTGA TGAGCACAAC AGGAGATATC CAGTCCATGG TCCTGTGGAT GGTAAAACCC 
     1441 TAGGCCTTTT ACGAAAGAAG GAAAAGAGAG CTACTTCCTG GCCGGACCTC ATTGCCAAGG 
     1501 TTTTCCGGAT CGATGTGAAG GCAGATGTTG ACTCGATCCA CCCCACTGAG TTCTGCCATA 
     1561 ACTGCTGGAG CATCATGCAC AGGAAGTTTA GCAGTGCCCC ATGTGAGGTT TACTTCCCGA 
     1621 GGAACGTGAC CATGGAGTGG CACCCCCACA CACCATCCTG TGACATCTGC AACACTGCCC 
     1681 GTCGGGGACT CAAGAGGAAG AGTCTTCAGC CAAACTTGCA GCTCAGCAAA AAACTCAAAA 
     1741 CTGTGCTTGA CCAAGCAAGA CAAGCCCGTC AGCGCAAGAG AAGAGCTCAG GCAAGGATCA 
     1801 GCAGCAAGGA TGTCATGAAG AAGATCGCCA ACTGCAGTAA GATACATCTT AGTACCAAGC 
     1861 TCCTTGCAGT GGACTTCCCA GAGCACTTTG TGAAATCCAT CTCCTGCCAG ATCTGTGAAC 
     1921 ACATTCTGGC TGACCCTGTG GAGACCAACT GTAAGCATGT CTTTTGCCGG GTCTGCATTC 
     1981 TCAGATGCCT CAAAGTCATG GGCAGCTATT GTCCCTCTTG CCGATATCCA TGCTTCCCTA 
     2041 CTGACCTGGA GAGTCCAGTG AAGTCCTTTC TGAGCGTCTT GAATTCCCTG ATGGTGAAAT 
     2101 GTCCAGCAAA AGAGTGCAAT GAGGAGGTCA GTTTGGAAAA ATATAATCAC CACATCTCAA 
     2161 GTCACAAGGA ATCAAAAGAG ATTTTTGTGC ACATTAATAA AGGGGGCCGG CCCCGCCAAC 
     2221 ATCTTCTGTC GCTGACTCGG AGAGCTCAGA AGCACCGGCT GAGGGAGCTC AAGCTGCAAG 
     2281 TCAAAGCCTT TGCTGACAAA GAAGAAGGTG GAGATGTGAA GTCCGTGTGC ATGACCTTGT 
     2341 TCCTGCTGGC TCTGAGGGCG AGGAATGAGC ACAGGCAAGC TGATGAGCTG GAGGCCATCA 
     2401 TGCAGGGAAA GGGCTCTGGC CTGCAGCCAG CTGTTTGCTT GGCCATCCGT GTCAACACCT 
     2461 TCCTCAGCTG CAGTCAGTAC CACAAGATGT ACAGGACTGT GAAAGCCATC ACAGGGAGAC 
     2521 AGATTTTTCA GCCTTTGCAT GCCCTTCGGA ATGCTGAGAA GGTACTTCTG CCAGGCTACC 
     2581 ACCACTTTGA GTGGCAGCCA CCTCTGAAGA ATGTGTCTTC CAGCACTGAT GTTGGCATTA 
     2641 TTGATGGGCT GTCTGGACTA TCATCCTCTG TGGATGATTA CCCAGTGGAC ACCATTGCAA 
     2701 AGAGGTTCCG CTATGATTCA GCTTTGGTGT CTGCTTTGAT GGACATGGAA GAAGACATCT 
     2761 TGGAAGGCAT GAGATCCCAA GACCTTGATG ATTACCTGAA TGGCCCCTTC ACTGTGGTGG 
     2821 TGAAGGAGTC TTGTGATGGA ATGGGAGACG TGAGTGAGAA GCATGGGAGT GGGCCTGTAG 
     2881 TTCCAGAAAA GGCAGTCCGT TTTTCATTCA CAATCATGAA AATTACTATT GCCCACAGCT 
     2941 CTCAGAATGT GAAAGTATTT GAAGAAGCCA AACCTAACTC TGAACTGTGT TGCAAGCCAT 
     3001 TGTGCCTTAT GCTGGCAGAT GAGTCTGACC ACGAGACGCT GACTGCCATC CTGAGTCCTC 
     3061 TCATTGCTGA GAGGGAGGCC ATGAAGAGCA GTGAATTAAT GCTTGAGCTG GGAGGCATTC 
     3121 TCCGGACTTT CAAGTTCATC TTCAGGGGCA CCGGCTATGA TGAAAAACTT GTGCGGGAAG 
     3181 TGGAAGGCCT CGAGGCTTCT GGCTCAGTCT ACATTTGTAC TCTTTGTGAT GCCACCCGTC 
     3241 TGGAAGCCTC TCAAAATCTT GTCTTCCACT CTATAACCAG AAGCCATGCT GAGAACCTGG 
     3301 AACGTTATGA GGTCTGGCGT TCCAACCCTT ACCATGAGTC TGTGGAAGAA CTGCGGGATC 
     3361 GGGTGAAAGG GGTCTCAGCT AAACCTTTCA TTGAGACAGT CCCTTCCATA GATGCACTCC 
     3421 ACTGTGACAT TGGCAATGCA GCTGAGTTCT ACAAGATCTT CCAGCTAGAG ATAGGGGAAG 
     3481 TGTATAAGAA TCCCAATGCT TCCAAAGAGG AAAGGAAAAG GTGGCAGGCC ACACTGGACA 
     3541 AGCATCTCCG GAAGAAGATG AACCTCAAAC CAATCATGAG GATGAATGGC AACTTTGCCA 
     3601 GGAAGCTCAT GACCAAAGAG ACTGTGGATG CAGTTTGTGA GTTAATTCCT TCCGAGGAGA 
     3661 GGCACGAGGC TCTGAGGGAG CTGATGGATC TTTACCTGAA GATGAAACCA GTATGGCGAT 
     3721 CATCATGCCC TGCTAAAGAG TGCCCAGAAT CCCTCTGCCA GTACAGTTTC AATTCACAGC 
     3781 GTTTTGCTGA GCTCCTTTCT ACGAAGTTCA AGTATAGGTA TGAGGGAAAA ATCACCAATT 
     3841 ATTTTCACAA AACCCTGGCC CATGTTCCTG AAATTATTGA GAGGGATGGC TCCATTGGGG 
     3901 CATGGGCAAG TGAGGGAAAT GAGTCTGGTA ACAAACTGTT TAGGCGCTTC CGGAAAATGA 
     3961 ATGCCAGGCA GTCCAAATGC TATGAGATGG AAGATGTCCT GAAACACCAC TGGTTGTACA 
     4021 CCTCCAAATA CCTCCAGAAG TTTATGAATG CTCATAATGC ATTAAAAACC TCTGGGTTTA 
     4081 CCATGAACCC TCAGGCAAGC TTAGGGGACC CATTAGGCAT AGAGGACTCT CTGGAAAGCC 
     4141 AAGATTCAAT GGAATTTACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     4201 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     4261 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     4321 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     4381 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     4441 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     4501 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     4561 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     4621 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     4681 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     4741 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     4801 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     4861 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     4921 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     4981 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     5041 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     5101 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     5161 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     5221 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     5281 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     5341 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     5401 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     5461 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     5521 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     5581 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     5641 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     5701 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     5761 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     5821 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     5881 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     5941 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     6001 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     6061 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     6121 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     6181 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     6241 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     6301 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     6361 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     6421 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     6481 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     6541 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     6601 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     6661 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     6721 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     6781 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     6841 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     6901 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     6961 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     7021 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     7081 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     7141 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     7201 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     7261 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7321 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7381 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     7441 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     7501 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     7561 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     7621 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     7681 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     7741 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     7801 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     7861 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     7921 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     7981 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//