LOCUS       RC216481                9300 bp    DNA     circular  SYN 
DEFINITION  NLRP1 (Myc-DDK-tagged)-Human NLR family, pyrin domain containing 1 (NLRP1), transcript variant 1
ACCESSION   NM_033004
VERSION     NM_033004.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5457..5548 
                     /label="Myc-DDK"  
     misc_feature    5578..6163 
                     /label="PolyA signal"  
     misc_feature    5635..5654 
                     /label="Restriction Enzyme"  
     misc_feature    6439..7111 
                     /label="ColE1"  
     misc_feature    7541..8335 
                     /label="Kan/Neo"  
     misc_feature    8370..8672 
                     /label="SV40 ori"  
     misc_feature    8758..9235 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5439..5445 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5439 
                     /label="NLRP1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTGGCGGA GCCTGGGGCC GCCTGGCCTG TTACTTGGAG TTCCTGAAGA 
     1081 AGGAGGAGCT GAAGGAGTTC CAGCTTCTGC TCGCCAATAA AGCGCACTCC AGGAGCTCTT 
     1141 CGGGTGAGAC ACCCGCTCAG CCAGAGAAGA CGAGTGGCAT GGAGGTGGCC TCGTACCTGG 
     1201 TGGCTCAGTA TGGGGAGCAG CGGGCCTGGG ACCTAGCCCT CCATACCTGG GAGCAGATGG 
     1261 GGCTGAGGTC ACTGTGCGCC CAAGCCCAGG AAGGGGCAGG CCACTCTCCC TCATTCCCCT 
     1321 ACAGCCCAAG TGAACCCCAC CTGGGGTCTC CCAGCCAACC CACCTCCACC GCAGTGCTAA 
     1381 TGCCCTGGAT CCATGAATTG CCGGCGGGGT GCACCCAGGG CTCAGAGAGA AGGGTTTTGA 
     1441 GACAGCTGCC TGACACATCT GGACGCCGCT GGAGAGAAAT CTCTGCCTCA CTCCTCTACC 
     1501 AAGCTCTTCC AAGCTCCCCA GACCATGAGT CTCCAAGCCA GGAGTCACCC AACGCCCCCA 
     1561 CATCCACAGC AGTGCTGGGG AGCTGGGGAT CCCCACCTCA GCCCAGCCTA GCACCCAGAG 
     1621 AGCAGGAGGC TCCTGGGACC CAATGGCCTC TGGATGAAAC GTCAGGAATT TACTACACAG 
     1681 AAATCAGAGA AAGAGAGAGA GAGAAATCAG AGAAAGGCAG GCCCCCATGG GCAGCGGTGG 
     1741 TAGGAACGCC CCCACAGGCG CACACCAGCC TACAGCCCCA CCACCACCCA TGGGAGCCTT 
     1801 CTGTGAGAGA GAGCCTCTGT TCCACATGGC CCTGGAAAAA TGAGGATTTT AACCAAAAAT 
     1861 TCACACAGCT GCTACTTCTA CAAAGACCTC ACCCCAGAAG CCAAGATCCC CTGGTCAAGA 
     1921 GAAGCTGGCC TGATTATGTG GAGGAGAATC GAGGACATTT AATTGAGATC AGAGACTTAT 
     1981 TTGGCCCAGG CCTGGATACC CAAGAACCTC GCATAGTCAT ACTGCAGGGG GCTGCTGGAA 
     2041 TTGGGAAGTC AACACTGGCC AGGCAGGTGA AGGAAGCCTG GGGGAGAGGC CAGCTGTATG 
     2101 GGGACCGCTT CCAGCATGTC TTCTACTTCA GCTGCAGAGA GCTGGCCCAG TCCAAGGTGG 
     2161 TGAGTCTCGC TGAGCTCATC GGAAAAGATG GGACAGCCAC TCCGGCTCCC ATTAGACAGA 
     2221 TCCTGTCTAG GCCAGAGCGG CTGCTCTTCA TCCTCGATGG TGTAGATGAG CCAGGATGGG 
     2281 TCTTGCAGGA GCCGAGTTCT GAGCTCTGTC TGCACTGGAG CCAGCCACAG CCGGCGGATG 
     2341 CACTGCTGGG CAGTTTGCTG GGGAAAACTA TACTTCCCGA GGCATCCTTC CTGATCACGG 
     2401 CTCGGACCAC AGCTCTGCAG AACCTCATTC CTTCTTTGGA GCAGGCACGT TGGGTAGAGG 
     2461 TCCTGGGGTT CTCTGAGTCC AGCAGGAAGG AATATTTCTA CAGATATTTC ACAGATGAAA 
     2521 GGCAAGCAAT TAGAGCCTTT AGGTTGGTCA AATCAAACAA AGAGCTCTGG GCCCTGTGTC 
     2581 TTGTGCCCTG GGTGTCCTGG CTGGCCTGCA CTTGCCTGAT GCAGCAGATG AAGCGGAAGG 
     2641 AAAAACTCAC ACTGACTTCC AAGACCACCA CAACCCTCTG TCTACATTAC CTTGCCCAGG 
     2701 CTCTCCAAGC TCAGCCATTG GGACCCCAGC TCAGAGACCT CTGCTCTCTG GCTGCTGAGG 
     2761 GCATCTGGCA AAAAAAGACC CTTTTCAGTC CAGATGACCT CAGGAAGCAT GGGTTAGATG 
     2821 GGGCCATCAT CTCCACCTTC TTGAAGATGG GTATTCTTCA AGAGCACCCC ATCCCTCTGA 
     2881 GCTACAGCTT CATTCACCTC TGTTTCCAAG AGTTCTTTGC AGCAATGTCC TATGTCTTGG 
     2941 AGGATGAGAA GGGGAGAGGT AAACATTCTA ATTGCATCAT AGATTTGGAA AAGACGCTAG 
     3001 AAGCATATGG AATACATGGC CTGTTTGGGG CATCAACCAC ACGTTTCCTA TTGGGCCTGT 
     3061 TAAGTGATGA GGGGGAGAGA GAGATGGAGA ACATCTTTCA CTGCCGGCTG TCTCAGGGGA 
     3121 GGAACCTGAT GCAGTGGGTC CCGTCCCTGC AGCTGCTGCT GCAGCCACAC TCTCTGGAGT 
     3181 CCCTCCACTG CTTGTACGAG ACTCGGAACA AAACGTTCCT GACACAAGTG ATGGCCCATT 
     3241 TCGAAGAAAT GGGCATGTGT GTAGAAACAG ACATGGAGCT CTTAGTGTGC ACTTTCTGCA 
     3301 TTAAATTCAG CCGCCACGTG AAGAAGCTTC AGCTGATTGA GGGCAGGCAG CACAGATCAA 
     3361 CATGGAGCCC CACCATGGTA GTCCTGTTCA GGTGGGTCCC AGTCACAGAT GCCTATTGGC 
     3421 AGATTCTCTT CTCCGTCCTC AAGGTCACCA GAAACCTGAA GGAGCTGGAC CTAAGTGGAA 
     3481 ACTCGCTGAG CCACTCTGCA GTGAAGAGTC TTTGTAAGAC CCTGAGACGC CCTCGCTGCC 
     3541 TCCTGGAGAC CCTGCGGTTG GCTGGCTGTG GCCTCACAGC TGAGGACTGC AAGGACCTTG 
     3601 CCTTTGGGCT GAGAGCCAAC CAGACCCTGA CCGAGCTGGA CCTGAGCTTC AATGTGCTCA 
     3661 CGGATGCTGG AGCCAAACAC CTTTGCCAGA GACTGAGACA GCCGAGCTGC AAGCTACAGC 
     3721 GACTGCAGCT GGTCAGCTGT GGCCTCACGT CTGACTGCTG CCAGGACCTG GCCTCTGTGC 
     3781 TTAGTGCCAG CCCCAGCCTG AAGGAGCTAG ACCTGCAGCA GAACAACCTG GATGACGTTG 
     3841 GCGTGCGACT GCTCTGTGAG GGGCTCAGGC ATCCTGCCTG CAAACTCATA CGCCTGGGGC 
     3901 TGGACCAGAC AACTCTGAGT GATGAGATGA GGCAGGAACT GAGGGCCCTG GAGCAGGAGA 
     3961 AACCTCAGCT GCTCATCTTC AGCAGACGGA AACCAAGTGT GATGACCCCT ACTGAGGGCC 
     4021 TGGATACGGG AGAGATGAGT AATAGCACAT CCTCACTCAA GCGGCAGAGA CTCGGATCAG 
     4081 AGAGGGCGGC TTCCCATGTT GCTCAGGCTA ATCTCAAACT CCTGGACGTG AGCAAGATCT 
     4141 TCCCAATTGC TGAGATTGCA GAGGAAAGCT CCCCAGAGGT AGTACCGGTG GAACTCTTGT 
     4201 GCGTGCCTTC TCCTGCCTCT CAAGGGGACC TGCATACGAA GCCTTTGGGG ACTGACGATG 
     4261 ACTTCTGGGG CCCCACGGGG CCTGTGGCTA CTGAGGTAGT TGACAAAGAA AAGAACTTGT 
     4321 ACCGAGTTCA CTTCCCTGTA GCTGGCTCCT ACCGCTGGCC CAACACGGGT CTCTGCTTTG 
     4381 TGATGAGAGA AGCGGTGACC GTTGAGATTG AATTCTGTGT GTGGGACCAG TTCCTGGGTG 
     4441 AGATCAACCC ACAGCACAGC TGGATGGTGG CAGGGCCTCT GCTGGACATC AAGGCTGAGC 
     4501 CTGGAGCTGT GGAAGCTGTG CACCTCCCTC ACTTTGTGGC TCTCCAAGGG GGCCATGTGG 
     4561 ACACATCCCT GTTCCAAATG GCCCACTTTA AAGAGGAGGG GATGCTCCTG GAGAAGCCAG 
     4621 CCAGGGTGGA GCTGCATCAC ATAGTTCTGG AAAACCCCAG CTTCTCCCCC TTGGGAGTCC 
     4681 TCCTGAAAAT GATCCATAAT GCCCTGCGCT TCATTCCCGT CACCTCTGTG GTGTTGCTTT 
     4741 ACCACCGCGT CCATCCTGAG GAAGTCACCT TCCACCTCTA CCTGATCCCA AGTGACTGCT 
     4801 CCATTCGGAA GGCCATAGAT GATCTAGAAA TGAAATTCCA GTTTGTGCGA ATCCACAAGC 
     4861 CACCCCCGCT GACCCCACTT TATATGGGCT GTCGTTACAC TGTGTCTGGG TCTGGTTCAG 
     4921 GGATGCTGGA AATACTCCCC AAGGAACTGG AGCTCTGCTA TCGAAGCCCT GGAGAAGACC 
     4981 AGCTGTTCTC GGAGTTCTAC GTTGGCCACT TGGGATCAGG GATCAGGCTG CAAGTGAAAG 
     5041 ACAAGAAAGA TGAGACTCTG GTGTGGGAGG CCTTGGTGAA ACCAGGAGAT CTCATGCCTG 
     5101 CAACTACTCT GATCCCTCCA GCCCGCATAG CCGTACCTTC ACCTCTGGAT GCCCCGCAGT 
     5161 TGCTGCACTT TGTGGACCAG TATCGAGAGC AGCTGATAGC CCGAGTGACA TCGGTGGAGG 
     5221 TTGTCTTGGA CAAACTGCAT GGACAGGTGC TGAGCCAGGA GCAGTACGAG AGGGTGCTGG 
     5281 CTGAGAACAC GAGGCCCAGC CAGATGCGGA AGCTGTTCAG CTTGAGCCAG TCCTGGGACC 
     5341 GGAAGTGCAA AGATGGACTC TACCAAGCCC TGAAGGAGAC CCATCCTCAC CTCATTATGG 
     5401 AACTCTGGGA GAAGGGCAGC AAAAAGGGAC TCCTGCCACT CAGCAGCACG CGTACGCGGC 
     5461 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     5521 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     5581 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     5641 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     5701 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     5761 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     5821 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     5881 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     5941 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     6001 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     6061 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     6121 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     6181 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     6241 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     6301 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     6361 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     6421 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     6481 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     6541 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     6601 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     6661 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     6721 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     6781 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     6841 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     6901 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     6961 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     7021 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     7081 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     7141 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     7201 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     7261 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     7321 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     7381 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     7441 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     7501 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     7561 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     7621 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     7681 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     7741 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     7801 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     7861 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     7921 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     7981 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     8041 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     8101 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     8161 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     8221 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     8281 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     8341 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     8401 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     8461 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     8521 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     8581 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     8641 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     8701 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     8761 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     8821 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     8881 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     8941 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     9001 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     9061 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     9121 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     9181 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     9241 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//