LOCUS       RC216328                7179 bp    DNA     circular  SYN 
DEFINITION  EMILIN3 (Myc-DDK-tagged)-Human elastin microfibril interfacer 3 (EMILIN3)
ACCESSION   NM_052846
VERSION     NM_052846.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3336..3427 
                     /label="Myc-DDK"  
     misc_feature    3457..4042 
                     /label="PolyA signal"  
     misc_feature    3514..3533 
                     /label="Restriction Enzyme"  
     misc_feature    4318..4990 
                     /label="ColE1"  
     misc_feature    5420..6214 
                     /label="Kan/Neo"  
     misc_feature    6249..6551 
                     /label="SV40 ori"  
     misc_feature    6637..7114 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3318..3324 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3318 
                     /label="EMILIN3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCCGCCGC CGCCTGCTCG TCTGGCTGTG CGCCGTCGCG GCGCTGCTCT 
     1081 CGGGGGCGCA GGCCAGGGGC ACCCCGCTCC TGGCGCGGCC TGCGCCGCCC GGTGCCTCCC 
     1141 GCTACAGTCT CTACACGACG GGATGGCGCC CGCGGCTGCG CCCGGGGCCG CACAAGGCCC 
     1201 TCTGTGCCTA TGTGGTGCAC AGGAATGTGA CCTGCATCCT ACAGGAGGGA GCGGAGAGCT 
     1261 ACGTAAAGGC TGAATACCGG CAGTGTAGAT GGGGGCCCAA GTGCCCCGGG ACAGTCACGT 
     1321 ACCGCACAGT ACTCAGACCC AAATACAAGG TTGGCTACAA GACAGTGACA GACCTCGCCT 
     1381 GGCGTTGCTG TCCCGGCTTC ACTGGGAAAC GCTGCCCTGA GCACCTCACG GACCATGGGG 
     1441 CTGCCTCACC CCAGCTGGAG CCTGAGCCTC AGATTCCTTC AGGGCAGCTG GACCCAGGCC 
     1501 CCAGGCCCCC TTCCTACAGC AGAGCAGCCC CCAGCCCTCA TGGAAGGAAA GGCCCAGGGC 
     1561 TGTTTGGTGA GCGGCTGGAA CGCCTGGAGG GTGATGTCCA GCGCCTGGCT CAAACATATG 
     1621 GTACCCTCAG TGGCCTGGTG GCTAGCCACG AGGATCCCAA CAGGATGACT GGTGGCCCCA 
     1681 GGGCTCCTGC TGTCCCTGTG GGCTTTGGGG TCATCCCTGA GGGGCTTGTG GGCCCAGGAG 
     1741 ACAGAGCCAG AGGGCCACTA ACACCTCCCT TAGACGAGAT CCTAAGCAAG GTGACAGAGG 
     1801 TGAGCAACAC TCTTCAGACC AAGGTGCAGC TTCTAGACAA GGTGCATGGG CTGGCACTTG 
     1861 GCCATGAGGC CCACCTGCAG CGGCTGCGGG AAGCCCCACC ATCCCCGCTC ACCTCCCTGG 
     1921 CCCTGCTGGA GGAGTACGTG GACCGACGGC TGCACCGACT CTGGGGGAGC CTGCTGGATG 
     1981 GCTTTGAGCA GAAGCTGCAA GGCGTCCAGA GTGAGTGTGA CCTGCGGGTG CAGGAGGTAC 
     2041 GGCGGCAATG TGAGGAGGGT CAGGCCGCCA GCCGGAGGCT GCACCAGAGC CTTGATGGCC 
     2101 GGGAGCTGGC CCTGCGCCAG GAGCTGTCAC AGCTGGGCAG CCAGCTGCAG GGCCTGAGCG 
     2161 TGTCTGGCAG GGGCAGCTGC TGTGGGCAAC TAGCCTTGAT CAATGCCCGT ATGGATGGCC 
     2221 TTGAGAGGGC CCTGCAGGCA GTCACCGAGA CCCAAAGGGG CCCCGGTGCC CCGGCCGGGG 
     2281 ATGAGCTTAC GAGGCTCTCT GCTGCCATGC TCGAGGGAGG TGTGGACGGG CTGCTTGAGG 
     2341 GTCTGGAGAC GCTCAATGGG ACAGAGGGTG GAGCAAGGGG ATGCTGTCTG AGGTTGGACA 
     2401 TGGGGGGGTG GGGAGTGGGC GGCTTTGGGA CCATGCTGGA AGAGCGCGTG CAGAGCCTCG 
     2461 AGGAGCGCCT AGCAACATTG GCTGGGGAGC TAAGCCATGA CAGCGCCTCT CCGGGCAGGT 
     2521 CAGCTCGGCC CCTTGTACAG ACAGAGCTGG CTGTGCTAGA GCAACGGTTG GTCTCACTGG 
     2581 AGACTTCGTG CACCCCGAGC ACCACCTCAG CCATCCTGGA CAGCCTCGTG GCAGAGGTGA 
     2641 AGGCCTGGCA GAGCCGGAGC GAGGCCCTCC TACGCCAGGT GGCCAGCCAC GCAGCACTGC 
     2701 TCCAGCAGCT CAATGGCACT GTGGCCGAGG TCCAGGGACA GCTGGCAGAA GGGACGGGCA 
     2761 GCTCACTTCA AGGCGAGATC ACTCTGCTCA AGGTCAATCT GAACTCAGTG AGCAAGTCGC 
     2821 TCACAGGCCT CAGTGACTCT GTCAGCCAGT ACTCTGATGC CTTCTTGGCT GCCAACACGT 
     2881 CCCTGGATGA GCGGGAACGC AAGGTGGAAG CCGAAGTCCA GGCCATCCAG GAGCAGGTCA 
     2941 GCAGCCAAGG CTCCAGGCTT CAGGCTGGCC ACAGGCAGGT CCTGAACCTG CGTGGGGAGC 
     3001 TGGAGCAACT CAAGGCTGGT GTGGCCAAGG TGGCCAGTGG GCTGAGCCGC TGCCAGGACA 
     3061 CAGCCCAGAA ACTTCAGCAC ACAGTGGGAC ACTTTGACCA GCGGGTGGCA CAAGTGGAGG 
     3121 GTGCGTGCAG GAGGCTGGGC CTGCTGGCCG CGGGCCTGGA CAGCTTGCCC ACTGAGCCAC 
     3181 TGAGGCCCAG AGAGGGCCTG TGGAGCCATG TGGACCAGCT GAATCGTACG CTGGCCCAGC 
     3241 ACACGCAGGA CATTGCCCGC CTCCGGGATG ACCTACTGGA CTGCCAGGCC CAGCTGGCTG 
     3301 AGCAGGTGCG GCCAGGGCAA GCCAACACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     3361 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     3421 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     3481 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     3541 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     3601 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     3661 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     3721 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     3781 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     3841 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     3901 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     3961 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     4021 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     4081 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     4141 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     4201 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     4261 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     4321 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     4381 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     4441 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     4501 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     4561 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     4621 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     4681 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     4741 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     4801 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     4861 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     4921 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     4981 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     5041 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     5101 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     5161 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     5221 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     5281 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     5341 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     5401 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     5461 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     5521 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     5581 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     5641 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     5701 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     5761 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     5821 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     5881 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     5941 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     6001 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     6061 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     6121 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     6181 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     6241 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     6301 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     6361 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     6421 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     6481 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     6541 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     6601 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     6661 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     6721 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     6781 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     6841 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     6901 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     6961 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     7021 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     7081 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     7141 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//