LOCUS       RC216318                8697 bp    DNA     circular  SYN 
DEFINITION  DIAPH1 (Myc-DDK-tagged)-Human diaphanous homolog 1 (Drosophila) (DIAPH1), transcript variant 1
ACCESSION   NM_005219
VERSION     NM_005219.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4854..4945 
                     /label="Myc-DDK"  
     misc_feature    4975..5560 
                     /label="PolyA signal"  
     misc_feature    5032..5051 
                     /label="Restriction Enzyme"  
     misc_feature    5836..6508 
                     /label="ColE1"  
     misc_feature    6938..7732 
                     /label="Kan/Neo"  
     misc_feature    7767..8069 
                     /label="SV40 ori"  
     misc_feature    8155..8632 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4836..4842 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4836 
                     /label="DIAPH1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCCGCCC GGCGGGAGCC TGGGGCCCGG CCGCGGGACC CGGGACAAGA 
     1081 AGAAGGGCCG GAGCCCAGAT GAGCTGCCCT CGGCGGGCGG CGACGGCGGC AAATCTAAGA 
     1141 AATTTACTCT GAAGCGGCTC ATGGCAGATG AGCTGGAGAG ATTTACCAGC ATGAGAATTA 
     1201 AGAAGGAGAA GGAAAAGCCC AATTCTGCTC ATAGAAATTC TTCTGCATCA TATGGGGATG 
     1261 ATCCCACAGC ACAGTCATTG CAAGATGTTT CAGATGAACA AGTGCTGGTT CTCTTTGAAC 
     1321 AGATGCTGCT GGATATGAAC CTGAATGAGG AGAAACAGCA ACCTTTGAGG GAGAAGGACA 
     1381 TCATCATCAA GAGGGAGATG GTGTCCCAAT ACTTGTACAC CTCCAAGGCT GGCATGAGCC 
     1441 AGAAGGAGAG CTCTAAGTCT GCCATGATGT ATATTCAGGA GTTGAGGTCA GGCTTGCGGG 
     1501 ATATGCCTCT GCTCAGCTGC CTGGAGTCCC TTCGTGTGTC TCTCAACAAC AACCCTGTCA 
     1561 GTTGGGTGCA AACATTTGGT GCTGAAGGCT TGGCCTCCTT ATTGGACATT CTTAAACGAC 
     1621 TTCATGATGA GAAAGAAGAG ACTGCTGGGA GTTACGATAG CCGGAACAAG CATGAGATCA 
     1681 TTCGCTGCTT GAAAGCTTTT ATGAACAACA AGTTTGGAAT CAAGACCATG TTGGAGACAG 
     1741 AAGAAGGAAT CCTACTGCTG GTCAGAGCCA TGGATCCTGC TGTTCCCAAC ATGATGATTG 
     1801 ATGCAGCTAA GCTGCTTTCT GCTCTTTGTA TTCTACCGCA GCCAGAGGAC ATGAATGAAA 
     1861 GGGTTTTGGA GGCAATGACA GAAAGAGCTG AGATGGATGA AGTGGAACGT TTCCAGCCGC 
     1921 TGCTGGATGG ATTAAAAAGT GGAACCACTA TTGCACTGAA GGTTGGATGC CTACAGCTGA 
     1981 TCAATGCTCT CATCACACCA GCGGAGGAAC TTGACTTCCG AGTTCACATC AGAAGTGAAC 
     2041 TGATGCGTTT GGGGCTACAT CAGGTGTTGC AGGACCTTCG AGAGATTGAA AATGAAGATA 
     2101 TGAGAGTGCA ACTAAATGTG TTTGATGAAC AAGGGGAAGA GGATTCCTAT GACCTGAAGG 
     2161 GACGGCTGGA TGACATTCGC ATGGAGATGG ATGACTTTAA TGAAGTCTTT CAGATTCTCT 
     2221 TAAACACAGT GAAGGATTCA AAGGCAGAGC CACACTTCCT TTCCATCCTG CAGCACTTAC 
     2281 TCTTGGTCCG AAATGACTAT GAGGCCAGAC CTCAGTACTA TAAGTTGATT GAAGAATGTA 
     2341 TTTCCCAGAT AGTTCTGCAC AAGAACGGGG CTGATCCTGA CTTCAAGTGC CGGCACCTCC 
     2401 AGATTGAGAT TGAGGGATTA ATTGATCAAA TGATTGATAA GACAAAGGTG GAGAAATCTG 
     2461 AAGCCAAAGC TGCAGAGCTG GAAAAGAAGT TGGACTCAGA GTTAACAGCC CGACATGAGC 
     2521 TACAGGTGGA AATGAAAAAG ATGGAAAGTG ACTTTGAGCA GAAGCTTCAA GATCTTCAGG 
     2581 GAGAAAAAGA TGCACTGCAT TCTGAAAAGC AGCAAATTGC CACAGAGAAA CAGGACCTGG 
     2641 AAGCAGAGGT GTCCCAGCTC ACAGGAGAGG TTGCCAAGCT GACAAAGGAA CTGGAAGATG 
     2701 CCAAGAAAGA AATGGCTTCC CTCTCTGCGG CAGCTATTAC TGTACCTCCT TCTGTTCCTA 
     2761 GTCGTGCTCC TGTTCCCCCT GCCCCTCCTT TACCTGGTGA CTCTGGCACT ATTATTCCAC 
     2821 CACCACCTGC TCCTGGGGAT AGTACCACTC CTCCTCCTCC TCCTCCTCCT CCTCCTCCTC 
     2881 CACCTCCTTT GCCTGGGGGT GTTTGCATCT CCTCACCCCC TTCTTTACCT GGAGGTACTG 
     2941 CTATCTCTCC ACCCCCTCCT TTGTCTGGGG ATGCTACCAT CCCTCCACCC CCTCCTTTGC 
     3001 CTGAGGGTGT TGGCATCCCT TCACCCTCTT CTTTGCCTGG AGGTACTGCC ATCCCCCCAC 
     3061 CTCCTCCTTT GCCTGGGAGT GCTAGAATCC CCCCACCACC ACCTCCTTTG CCTGGGAGTG 
     3121 CTGGAATTCC CCCCCCACCT CCTCCCTTGC CTGGAGAAGC AGGAATGCCA CCTCCTCCTC 
     3181 CCCCTCTTCC TGGTGGTCCT GGAATCCCTC CACCTCCTCC ATTTCCCGGA GGCCCTGGCA 
     3241 TTCCTCCACC TCCACCCGGA ATGGGTATGC CTCCACCTCC CCCATTTGGA TTTGGAGTTC 
     3301 CTGCAGCCCC AGTTCTGCCA TTTGGATTAA CCCCCAAAAA GCTTTATAAG CCAGAGGTGC 
     3361 AGCTCCGGAG GCCAAACTGG TCCAAGCTTG TGGCTGAGGA CCTCTCCCAG GACTGCTTCT 
     3421 GGACAAAGGT GAAGGAGGAC CGCTTTGAGA ACAATGAACT TTTCGCCAAA CTTACCCTTA 
     3481 CCTTCTCTGC CCAGACCAAG ACTTCCAAAG CCAAGAAGGA TCAAGAAGGT GGAGAAGAAA 
     3541 AGAAATCTGT GCAAAAGAAA AAAGTAAAAG AGTTAAAGGT GTTGGATTCA AAGACAGCCC 
     3601 AGAATCTCTC AATCTTTTTG GGTTCCTTCC GCATGCCCTA TCAAGAGATT AAGAATGTCA 
     3661 TCCTGGAGGT GAATGAGGCT GTTCTGACTG AGTCTATGAT CCAGAACCTC ATTAAGCAAA 
     3721 TGCCAGAGCC AGAGCAGTTA AAAATGCTTT CTGAACTGAA GGATGAATAT GATGACCTGG 
     3781 CTGAGTCAGA GCAGTTTGGC GTGGTGATGG GCACTGTGCC CCGACTGCGG CCTCGCCTCA 
     3841 ATGCCATTCT CTTCAAGCTA CAATTCAGCG AGCAAGTGGA GAATATCAAG CCAGAGATTG 
     3901 TGTCTGTCAC TGCTGCATGT GAGGAGTTAC GTAAGAGTGA GAGCTTTTCC AATCTCCTAG 
     3961 AGATTACCTT GCTTGTTGGA AATTACATGA ATGCTGGCTC CAGAAATGCT GGTGCTTTTG 
     4021 GCTTCAATAT CAGCTTCCTC TGTAAGCTTC GAGACACCAA GTCCACAGAT CAGAAGATGA 
     4081 CGTTGTTACA CTTCTTGGCT GAGTTGTGTG AGAATGACTA TCCCGATGTC CTCAAGTTTC 
     4141 CAGACGAGCT TGCCCATGTG GAGAAAGCCA GCCGAGTTTC TGCTGAAAAC TTGCAAAAGA 
     4201 ACCTAGATCA GATGAAGAAA CAAATTTCTG ATGTGGAACG TGATGTTCAG AATTTCCCAG 
     4261 CTGCCACAGA TGAAAAAGAC AAGTTTGTTG AAAAAATGAC CAGCTTTGTG AAGGATGCAC 
     4321 AGGAACAGTA TAACAAGCTG CGGATGATGC ATTCTAACAT GGAGACCCTC TATAAGGAGC 
     4381 TGGGCGAGTA CTTCCTCTTT GACCCCAAGA AGTTGTCTGT TGAAGAATTT TTCATGGATC 
     4441 TTCACAATTT TCGGAATATG TTTTTGCAAG CAGTCAAGGA GAACCAGAAG CGGCGGGAGA 
     4501 CAGAAGAAAA GATGAGGCGA GCAAAACTAG CCAAGGAGAA GGCAGAGAAG GAGCGGCTAG 
     4561 AGAAGCAGCA GAAGAGAGAG CAACTCATAG ACATGAATGC AGAGGGCGAT GAGACAGGTG 
     4621 TGATGGACAG TCTTCTAGAA GCCCTGCAGT CAGGGGCAGC ATTCCGACGG AAGAGAGGGC 
     4681 CCCGTCAAGC CAACAGGAAG GCCGGGTGTG CAGTCACATC TCTGCTAGCT TCGGAGCTGA 
     4741 CCAAGGATGA TGCCATGGCT GCTGTTCCTG CCAAGGTGTC CAAGAACAGT GAGACATTCC 
     4801 CCACAATCCT TGAGGAAGCC AAGGAGTTGG TTGGCCGTGC AAGCACGCGT ACGCGGCCGC 
     4861 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     4921 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4981 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     5041 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     5101 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     5161 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     5221 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     5281 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     5341 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     5401 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     5461 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     5521 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5581 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     5641 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5701 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5761 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5821 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     5881 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     5941 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     6001 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     6061 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     6121 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     6181 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     6241 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     6301 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     6361 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     6421 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     6481 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     6541 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     6601 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6661 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6721 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6781 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6841 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     6901 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     6961 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     7021 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     7081 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     7141 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     7201 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     7261 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     7321 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     7381 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     7441 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     7501 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     7561 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     7621 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7681 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7741 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7801 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7861 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     7921 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7981 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     8041 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     8101 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     8161 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     8221 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     8281 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     8341 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     8401 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     8461 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     8521 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     8581 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     8641 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//