LOCUS       RC216272                8256 bp    DNA     circular  SYN 
DEFINITION  ANLN (Myc-DDK-tagged)-Human anillin, actin binding protein (ANLN)
ACCESSION   NM_018685
VERSION     NM_018685.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4413..4504 
                     /label="Myc-DDK"  
     misc_feature    4534..5119 
                     /label="PolyA signal"  
     misc_feature    4591..4610 
                     /label="Restriction Enzyme"  
     misc_feature    5395..6067 
                     /label="ColE1"  
     misc_feature    6497..7291 
                     /label="Kan/Neo"  
     misc_feature    7326..7628 
                     /label="SV40 ori"  
     misc_feature    7714..8191 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4395..4401 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4395 
                     /label="ANLN"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATCCGTTT ACGGAGAAAC TGCTGGAGCG AACCCGTGCC AGGCGAGAGA 
     1081 ATCTTCAGAG AAAAATGGCT GAGAGGCCCA CAGCAGCTCC AAGGTCTATG ACTCATGCTA 
     1141 AGCGAGCTAG ACAGCCACTT TCAGAAGCAA GTAACCAGCA GCCCCTCTCT GGTGGTGAAG 
     1201 AGAAATCTTG TACAAAACCA TCGCCATCAA AAAAACGCTG TTCTGACAAC ACTGAAGTAG 
     1261 AAGTTTCTAA CTTGGAAAAT AAACAACCAG TTGAGTCGAC ATCTGCAAAA TCTTGTTCTC 
     1321 CAAGTCCTGT GTCTCCTCAG GTGCAGCCAC AAGCAGCAGA TACCATCAGT GATTCTGTTG 
     1381 CTGTCCCGGC ATCACTGCTG GGCATGAGGA GAGGGCTGAA CTCAAGATTG GAAGCAACTG 
     1441 CAGCCTCCTC AGTTAAAACA CGTATGCAAA AACTTGCAGA GCAACGGCGC CGTTGGGATA 
     1501 ATGATGATAT GACAGATGAC ATTCCTGAAA GCTCACTCTT CTCACCAATG CCATCAGAGG 
     1561 AAAAGGCTGC TTCCCCTCCC AAACCTCTGC TTTCAAATGC CTCGGCAACT CCAGTTGGCA 
     1621 GAAGGGGCCG TCTGGCCAAT CTTGCTGCAA CTATTTGCTC CTGGGAAGAT GATGTAAATC 
     1681 ACTCATTTGC AAAACAAAAC AGTGTACAAG AACAGCCTGG TACCGCTTGT TTATCCAAAT 
     1741 TTTCCTCTGC AAGTGGAGCA TCTGCTAGGA TCAATAGCAG CAGTGTTAAG CAGGAAGCTA 
     1801 CATTCTGTTC CCAAAGGGAT GGCGATGCCT CTTTGAATAA AGCCCTATCC TCAAGTGCTG 
     1861 ATGATGCGTC TTTGGTTAAT GCCTCAATTT CCAGCTCTGT GAAAGCTACT TCTTCTCCAG 
     1921 TGAAATCTAC TACATCTATC ACTGATGCTA AAAGTTGTGA GGGACAAAAT CCTGAGCTAC 
     1981 TTCCAAAAAC TCCTATTAGT CCTCTGAAAA CGGGGGTATC GAAACCAATT GTGAAGTCAA 
     2041 CTTTATCCCA GACAGTTCCA TCCAAGGGAG AATTAAGTAG AGAAATTTGT CTGCAATCTC 
     2101 AATCTAAAGA CAAATCTACG ACACCAGGAG GAACAGGAAT TAAGCCTTTC CTGGAACGCT 
     2161 TTGGAGAGCG TTGTCAAGAA CATAGCAAAG AAAGTCCAGC TCGTAGCACA CCCCACAGAA 
     2221 CCCCCATTAT TACTCCAAAT ACAAAGGCCA TCCAAGAAAG ATTATTCAAG CAAGACACAT 
     2281 CTTCATCTAC TACCCATTTA GCACAACAGC TCAAGCAGGA ACGTCAAAAA GAACTAGCAT 
     2341 GTCTTCGTGG CCGATTTGAC AAGGGCAATA TATGGAGTGC AGAAAAAGGC GGAAACTCAA 
     2401 AAAGCAAACA ACTAGAAACC AAACAGGAAA CTCACTGTCA GAGCACTCCC CTCAAAAAAC 
     2461 ACCAAGGTGT TTCAAAAACT CAGTCACTTC CAGTAACAGA AAAGGTGACC GAAAACCAGA 
     2521 TACCAGCCAA AAATTCTAGT ACAGAACCTA AAGGTTTCAC TGAATGCGAA ATGACGAAAT 
     2581 CTAGCCCTTT GAAAATAACA TTGTTTTTAG AAGAGGACAA ATCCTTAAAA GTAACATCAG 
     2641 ACCCAAAGGT TGAGCAGAAA ATTGAAGTGA TACGTGAAAT TGAGATGAGT GTGGATGATG 
     2701 ATGATATCAA TAGTTCGAAA GTAATTAATG ACCTCTTCAG TGATGTCCTA GAGGAAGGTG 
     2761 AACTAGATAT GGAGAAGAGC CAAGAGGAGA TGGATCAAGC ATTAGCAGAA AGCAGCGAAG 
     2821 AACAGGAAGA TGCACTGAAT ATCTCCTCAA TGTCTTTACT TGCACCATTG GCACAAACAG 
     2881 TTGGTGTGGT AAGTCCAGAG AGTTTAGTGT CCACACCTAG ACTGGAATTG AAAGACACCA 
     2941 GCAGAAGTGA TGAAAGTCCA AAACCAGGAA AATTCCAAAG AACTCGTGTC CCTCGAGCTG 
     3001 AATCTGGTGA TAGCCTTGGT TCTGAAGATC GTGATCTTCT TTACAGCATT GATGCATATA 
     3061 GATCTCAAAG ATTCAAAGAA ACAGAACGTC CATCAATAAA GCAGGTGATT GTTCGGAAGG 
     3121 AAGATGTTAC TTCAAAACTG GATGAAAAAA ATAATGCCTT TCCTTGTCAA GTTAATATCA 
     3181 AACAGAAAAT GCAGGAACTC AATAACGAAA TAAATATGCA ACAGACAGTG ATCTATCAAG 
     3241 CTAGCCAGGC TCTTAACTGC TGTGTTGATG AAGAACATGG AAAAGGGTCC CTAGAAGAAG 
     3301 CTGAAGCAGA AAGACTTCTT CTAATTGCAA CTGGGAAGAG AACACTTTTG ATTGATGAAT 
     3361 TGAATAAATT GAAGAACGAA GGACCTCAGA GGAAGAATAA GGCTAGTCCC CAAAGTGAAT 
     3421 TTATGCCATC CAAAGGATCA GTTACTTTGT CAGAAATCCG CTTGCCTCTA AAAGCAGATT 
     3481 TTGTCTGCAG TACGGTTCAG AAACCAGATG CAGCAAATTA CTATTACTTA ATTATACTAA 
     3541 AAGCAGGAGC TGAAAATATG GTAGCCACAC CATTAGCAAG TACTTCAAAC TCTCTTAACG 
     3601 GTGATGCTCT GACATTCACT ACTACATTTA CTCTGCAAGA TGTATCCAAT GACTTTGAAA 
     3661 TAAATATTGA AGTTTACAGC TTGGTGCAAA AGAAAGATCC CTCAGGCCTT GATAAGAAGA 
     3721 AAAAAACATC CAAGTCCAAG GCTATTACTC CAAAGCGACT CCTCACATCT ATAACCACAA 
     3781 AAAGCAACAT TCATTCTTCA GTCATGGCCA GTCCAGGAGG TCTTAGTGCT GTGCGAACCA 
     3841 GCAACTTCGC CCTTGTTGGA TCTTACACAT TATCATTGTC TTCAGTAGGA AATACTAAGT 
     3901 TTGTTCTGGA CAAGGTCCCC TTTTTATCTT CTTTGGAAGG TCATATTTAT TTAAAAATAA 
     3961 AATGTCAAGT GAATTCCAGT GTTGAAGAAA GAGGTTTTCT AACCATATTT GAAGATGTTA 
     4021 GTGGTTTTGG TGCCTGGCAT CGAAGATGGT GTGTTCTTTC TGGAAACTGT ATATCTTATT 
     4081 GGACTTATCC AGATGATGAG AAACGCAAGA ATCCCATAGG AAGGATAAAT CTGGCTAATT 
     4141 GTACCAGTCG TCAGATAGAA CCAGCCAACA GAGAATTTTG TGCAAGACGC AACACTTTTG 
     4201 AATTAATTAC TGTCCGACCA CAAAGAGAAG ATGACCGAGA GACTCTTGTC AGCCAATGCA 
     4261 GGGACACACT CTGTGTTACC AAGAACTGGC TGTCTGCAGA TACTAAAGAA GAGCGGGATC 
     4321 TCTGGATGCA AAAACTCAAT CAAGTTCTTG TTGATATTCG CCTCTGGCAA CCTGATGCTT 
     4381 GCTACAAACC TATTGGAAAG CCTACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4441 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4501 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4561 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4621 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4681 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4741 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     4801 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     4861 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     4921 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     4981 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     5041 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5101 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5161 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     5221 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     5281 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     5341 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5401 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5461 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5521 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     5581 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     5641 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     5701 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     5761 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     5821 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     5881 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     5941 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     6001 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     6061 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     6121 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6181 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     6241 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6301 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6361 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6421 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6481 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6541 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     6601 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     6661 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     6721 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     6781 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     6841 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     6901 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     6961 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     7021 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     7081 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     7141 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7201 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     7261 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7321 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7381 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7441 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7501 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7561 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7621 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     7681 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     7741 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     7801 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     7861 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     7921 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     7981 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     8041 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     8101 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     8161 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     8221 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//