LOCUS       RC216239                8664 bp    DNA     circular  SYN 
DEFINITION  SHANK2 (Myc-DDK-tagged)-Human SH3 and multiple ankyrin repeat domains 2 (SHANK2), transcript variant 2
ACCESSION   NM_133266
VERSION     NM_133266.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4821..4912 
                     /label="Myc-DDK"  
     misc_feature    4942..5527 
                     /label="PolyA signal"  
     misc_feature    4999..5018 
                     /label="Restriction Enzyme"  
     misc_feature    5803..6475 
                     /label="ColE1"  
     misc_feature    6905..7699 
                     /label="Kan/Neo"  
     misc_feature    7734..8036 
                     /label="SV40 ori"  
     misc_feature    8122..8599 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4803..4809 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4803 
                     /label="SHANK2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATGATGAAC GTCCCCGGCG GAGGAGCGGC CGCGGTGATG ATGACGGGCT 
     1081 ACAATAATGG TCGCTGTCCC CGGAATTCTC TCTACAGTGA CTGCATTATT GAGGAGAAGA 
     1141 CGGTGGTCCT GCAGAAAAAA GACAATGAGG GCTTTGGATT CGTGCTTCGA GGGGCCAAAG 
     1201 CTGACACACC CATTGAAGAA TTCACACCAA CACCGGCTTT CCCAGCCCTA CAGTACCTGG 
     1261 AGTCCGTGGA TGAAGGTGGG GTGGCGTGGC AAGCCGGACT AAGGACCGGG GACTTCTTGA 
     1321 TTGAGGTTAA CAATGAGAAT GTTGTCAAAG TCGGCCACAG GCAGGTGGTG AACATGATCC 
     1381 GGCAGGGAGG GAATCACCTG GTCCTTAAGG TGGTCACGGT GACCAGGAAT CTGGACCCCG 
     1441 ACGACACCGC CAGGAAGAAA GCTCCCCCGC CTCCAAAGCG GGCACCGACC ACAGCCCTCA 
     1501 CCCTGCGCTC CAAGTCCATG ACCTCGGAGC TGGAGGAGCT CGTGGATAAA GCCTCGGTCC 
     1561 GGAAGAAGAA GGATAAACCC GAGGAGATAG TCCCGGCCTC CAAGCCCTCC CGCGCTGCTG 
     1621 AGAACATGGC TGTGGAACCG AGGGTGGCGA CCATCAAGCA GCGGCCCAGC AGCCGGTGCT 
     1681 TCCCGGCGGG CTCAGACATG AACTCTGTGT ACGAACGCCA AGGAATCGCC GTGATGACGC 
     1741 CCACTGTTCC TGGGAGCCCA AAAGCCCCGT TTCTGGGCAT CCCTCGAGGT ACGATGCGAA 
     1801 GGCAGAAATC AATAGACAGC AGAATCTTTC TATCAGGAAT AACAGAGGAA GAGCGGCAGT 
     1861 TTCTGGCTCC TCCAATGCTG AAGTTCACCA GAAGCCTGTC CATGCCGGAC ACCTCTGAGG 
     1921 ACATCCCCCC TCCACCGCAG TCTGTGCCCC CGTCCCCACC ACCACCTTCC CCAACCACTT 
     1981 ACAACTGCCC CAAGTCCCCA ACTCCAAGAG TCTACGGGAC GATTAAGCCT GCGTTCAATC 
     2041 AGAATTCTGC CGCCAAGGTG TCCCCCGCCA CCAGGTCCGA CACCGTGGCC ACCATGATGA 
     2101 GGGAGAAGGG GATGTACTTC AGGAGAGAGC TGGACCGCTA CTCCTTGGAC TCTGAAGACC 
     2161 TCTACAGTCG GAATGCCGGC CCGCAAGCCA ACTTCCGCAA CAAGAGAGGC CAGATGCCAG 
     2221 AAAACCCATA CTCAGAGGTG GGGAAGATCG CCAGCAAAGC CGTCTACGTC CCCGCCAAGC 
     2281 CCGCCAGGCG GAAGGGGATG CTGGTGAAGC AGTCCAACGT GGAGGACAGC CCCGAGAAGA 
     2341 CGTGCTCCAT CCCTATCCCG ACCATCATCG TGAAGGAGCC GTCCACCAGC AGCAGCGGCA 
     2401 AGAGCAGCCA GGGCAGCAGC ATGGAGATCG ACCCCCAGGC CCCGGAGCCA CCGAGCCAGC 
     2461 TGCGGCCTGA CGAAAGCCTG ACCGTCAGCA GCCCCTTTGC CGCCGCCATC GCCGGAGCCG 
     2521 TCCGCGACCG TGAGAAGCGG CTGGAAGCCA GGAGGAACTC CCCGGCCTTC CTCTCCACAG 
     2581 ACCTGGGGGA TGAGGATGTG GGCCTGGGGC CACCCGCCCC CAGGACGCGG CCCTCCATGT 
     2641 TCCCCGAGGA GGGGGATTTT GCTGACGAGG ACAGCGCTGA GCAGCTGTCA TCCCCCATGC 
     2701 CGAGTGCCAC GCCCAGGGAG CCCGAAAACC ATTTCGTGGG TGGCGCCGAG GCCAGTGCTC 
     2761 CGGGTGAGGC TGGGAGGCCG CTGAATTCCA CGTCCAAAGC CCAGGGGCCC GAGAGCAGCC 
     2821 CAGCAGTGCC CTCCGCGAGC AGCGGCACAG CCGGCCCCGG GAATTATGTC CACCCACTCA 
     2881 CAGGGCGGCT GCTTGATCCC AGCTCCCCGC TGGCCCTGGC ACTCTCCGCA AGGGACCGAG 
     2941 CCATGAAGGA GTCTCAACAG GGACCCAAAG GGGAGGCCCC CAAGGCCGAC CTCAACAAAC 
     3001 CTCTTTACAT TGATACCAAA ATGCGGCCCA GCCTGGATGC CGGCTTCCCT ACGGTCACCA 
     3061 GGCAGAACAC CCGGGGACCC CTGAGGCGGC AGGAGACGGA GAACAAGTAC GAGACCGACC 
     3121 TGGGCCGAGA CCGGAAAGGC GATGACAAGA AGAACATGCT GATCGACATC ATGGACACGT 
     3181 CCCAGCAGAA GTCGGCTGGC CTGCTGATGG TGCACACCGT GGACGCCACT AAGCTGGACA 
     3241 ACGCCCTGCA GGAAGAGGAC GAGAAGGCAG AGGTGGAGAT GAAGCCAGAC AGCTCGCCGT 
     3301 CCGAGGTGCC AGAAGGTGTT TCCGAAACCG AAGGTGCTTT ACAGATCTCC GCTGCCCCCG 
     3361 AGCCCACCAC CGTGCCCGGC AGAACCATCG TCGCGGTGGG CTCCATGGAA GAGGCGGTGA 
     3421 TTTTGCCATT CCGCATCCCT CCTCCCCCTC TGGCATCCGT GGACTTGGAT GAGGATTTTA 
     3481 TTTTTACAGA GCCATTGCCT CCTCCCCTGG AATTTGCAAA TAGTTTTGAT ATCCCCGATG 
     3541 ACCGGGCAGC TTCTGTCCCG GCTCTCTCAG ACTTAGTGAA GCAGAAGAAA AGCGACACCC 
     3601 CTCAGTCCCC TTCGTTGAAC TCCAGCCAAC CAACCAACTC TGCAGACAGC AAGAAGCCAG 
     3661 CCAGTCTTTC AAACTGTCTG CCTGCCTCAT TCCTGCCACC CCCTGAAAGC TTTGACGCCG 
     3721 TCGCCGACTC TGGGATCGAG GAGGTGGACA GCCGGAGTAG CAGCGACCAC CACCTCGAGA 
     3781 CGACCAGCAC TATCTCCACC GTGTCTAGCA TCTCCACCCT GTCTTCCGAA GGTGGAGAGA 
     3841 ATGTGGACAC CTGCACAGTC TATGCAGATG GGCAAGCATT TATGGTTGAC AAACCCCCAG 
     3901 TACCTCCTAA GCCAAAAATG AAGCCCATCA TTCACAAAAG CAATGCACTT TATCAAGACG 
     3961 CGCTCGTGGA AGAAGATGTA GATAGCTTTG TTATCCCCCC GCCCGCTCCC CCGCCCCCGC 
     4021 CGGGCAGTGC CCAGCCTGGG ATGGCCAAGG TTCTCCAGCC AAGGACCTCC AAGTTGTGGG 
     4081 GCGACGTCAC AGAGATCAAA AGCCCGATTC TCTCAGGCCC AAAGGCAAAC GTTATTAGTG 
     4141 AATTGAACTC TATCCTACAG CAAATGAACC GAGAGAAATT GGCAAAGCCG GGGGAAGGAC 
     4201 TGGATTCACC AATGGGAGCC AAGTCCGCCA GCCTCGCTCC AAGAAGCCCG GAGATCATGA 
     4261 GCACCATCTC AGGTACACGG AGCACGACGG TCACCTTCAC TGTTCGCCCC GGCACCTCCC 
     4321 AGCCCATCAC CCTGCAGAGC CGGCCCCCCG ACTATGAAAG CAGGACCTCA GGAACAAGAC 
     4381 GTGCCCCAAG CCCTGTGGTC TCGCCAACAG AGATGAACAA AGAGACCCTG CCCGCCCCCC 
     4441 TGTCTGCTGC CACCGCCTCT CCTTCTCCCG CTCTCTCAGA TGTCTTTAGC CTTCCAAGCC 
     4501 AGCCCCCTTC TGGGGATCTA TTTGGCTTGA ACCCAGCGGG ACGCAGTAGG TCGCCATCCC 
     4561 CCTCGATACT GCAACAGCCA ATCTCAAATA AGCCTTTTAC AACTAAACCT GTCCACCTGT 
     4621 GGACTAAACC AGATGTGGCC GATTGGCTGG AAAGTCTAAA CTTGGGTGAA CATAAAGAGG 
     4681 CCTTCATGGA CAATGAGATC GATGGCAGTC ACTTACCAAA CCTGCAGAAG GAGGACCTCA 
     4741 TCGATCTTGG GGTAACTCGA GTCGGGCACA GAATGAACAT AGAAAGGGCT TTGAAACAGC 
     4801 TGCTGGACAG AACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     4861 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4921 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4981 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     5041 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     5101 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     5161 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     5221 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     5281 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     5341 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     5401 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5461 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5521 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5581 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     5641 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     5701 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     5761 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     5821 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     5881 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     5941 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     6001 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     6061 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     6121 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     6181 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     6241 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     6301 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     6361 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     6421 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     6481 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6541 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6601 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6661 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6721 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     6781 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     6841 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6901 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     6961 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     7021 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     7081 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     7141 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     7201 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     7261 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     7321 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     7381 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     7441 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     7501 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7561 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7621 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7681 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7741 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     7801 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     7861 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7921 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7981 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     8041 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     8101 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     8161 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     8221 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     8281 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     8341 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     8401 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     8461 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     8521 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     8581 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     8641 TAACAAAAAT TTAACGCGAA TTTT
//