LOCUS       RC216225                8286 bp    DNA     circular  SYN 
DEFINITION  KCNT2 (Myc-DDK-tagged)-Human potassium channel, subfamily T, member 2 (KCNT2)
ACCESSION   NM_198503
VERSION     NM_198503.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4443..4534 
                     /label="Myc-DDK"  
     misc_feature    4564..5149 
                     /label="PolyA signal"  
     misc_feature    4621..4640 
                     /label="Restriction Enzyme"  
     misc_feature    5425..6097 
                     /label="ColE1"  
     misc_feature    6527..7321 
                     /label="Kan/Neo"  
     misc_feature    7356..7658 
                     /label="SV40 ori"  
     misc_feature    7744..8221 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4425..4431 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4425 
                     /label="KCNT2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGTTGATTTG GAGAGCGAAG TGCCCCCTCT GCCTCCCAGG TACAGGTTTC 
     1081 GAGATTTGCT GCTAGGGGAC CAAGGATGGC AAAACGACGA CAGGGTACAA GTTGAATTCT 
     1141 ATATGAATGA AAATACATTT AAAGAAAGAC TAAAATTATT TTTCATAAAA AACCAGAGAT 
     1201 CAAGTCTAAG GATACGCCTG TTCAATTTTT CTCTCAAATT ACTAAGCTGC TTATTATACA 
     1261 TAATCCGAGT ACTACTAGAA AACCCTTCAC AAGGAAATGA ATGGTCTCAT ATCTTTTGGG 
     1321 TGAACAGAAG TCTACCTTTG TGGGGCTTAC AGGTTTCAGT GGCATTGATA AGTCTGTTTG 
     1381 AAACAATATT ACTTGGTTAT CTTAGTTATA AGGGAAACAT CTGGGAACAG ATTTTACGAA 
     1441 TACCCTTCAT CTTGGAAATA ATTAATGCAG TTCCCTTCAT TATCTCAATA TTCTGGCCTT 
     1501 CCTTAAGGAA TCTATTTGTC CCAGTCTTTC TGAACTGTTG GCTTGCCAAA CATGCCTTGG 
     1561 AAAATATGAT TAATGATCTA CACAGAGCCA TTCAGCGTAC ACAGTCTGCA ATGTTTAATC 
     1621 AAGTTTTGAT TTTAATATCT ACATTACTAT GCCTTATCTT CACCTGCATT TGTGGGATCC 
     1681 AACATCTGGA ACGAATAGGA AAGAAGCTGA ATCTCTTTGA CTCCCTTTAT TTCTGCATTG 
     1741 TGACGTTTTC TACTGTGGGC TTCGGGGATG TCACTCCTGA AACATGGTCC TCCAAGCTTT 
     1801 TTGTAGTTGC TATGATTTGT GTTGCTCTTG TGGTTCTACC CATACAGTTT GAACAGCTGG 
     1861 CTTATTTGTG GATGGAGAGA CAAAAGTCAG GAGGAAACTA TAGTCGACAT AGAGCTCAAA 
     1921 CTGAAAAGCA TGTCGTCCTG TGTGTCAGCT CACTGAAGAT TGATTTACTT ATGGATTTTT 
     1981 TAAATGAATT CTATGCTCAT CCTAGGCTCC AGGATTATTA TGTGGTGATT TTGTGTCCTA 
     2041 CTGAAATGGA TGTACAGGTT CGAAGGGTAC TGCAGATTCC AATGTGGTCC CAACGAGTTA 
     2101 TCTACCTTCA AGGTTCAGCC CTTAAAGATC AAGACCTATT GAGAGCAAAG ATGGATGACG 
     2161 CTGAGGCCTG TTTTATTCTC AGTAGCCGTT GTGAAGTGGA TAGGACATCA TCTGATCACC 
     2221 AAACAATTTT GAGAGCATGG GCTGTGAAAG ATTTTGCTCC AAATTGTCCT TTGTATGTCC 
     2281 AGATATTAAA GCCTGAAAAT AAATTTCACA TCAAATTTGC TGATCATGTT GTTTGTGAAG 
     2341 AAGAGTTTAA ATACGCCATG TTAGCTTTAA ACTGTATATG CCCAGCAACA TCTACACTTA 
     2401 TTACACTACT GGTTCATACC TCTAGAGGGC AAGAAGGCCA GCAATCGCCA GAACAATGGC 
     2461 AGAAGATGTA CGGTAGATGC TCCGGGAATG AAGTCTACCA CATTGTTTTG GAAGAAAGTA 
     2521 CATTTTTTGC TGAATATGAA GGAAAGAGTT TTACATATGC CTCTTTCCAT GCACACAAAA 
     2581 AGTTTGGCGT CTGCTTGATT GGTGTTAGGA GGGAGGATAA TAAAAACATT TTGCTGAATC 
     2641 CAGGTCCTCG ATACATTATG AATTCTACAG ACATATGCTT TTATATTAAT ATTACCAAAG 
     2701 AAGAGAATTC AGCATTTAAA AACCAAGACC AGCAGAGAAA AAGCAATGTG TCCAGGTCGT 
     2761 TTTATCATGG ACCTTCCAGA TTACCTGTAC ATAGCATAAT TGCCAGCATG GGTACTGTGG 
     2821 CTATAGACTT GCAAGATACA AGCTGTAGAT CAGCAAGTGG CCCTACCCTG TCTCTTCCTA 
     2881 CAGAGGGAAG CAAAGAAATA AGAAGACCTA GCATTGCTCC TGTTTTAGAG GTTGCAGATA 
     2941 CATCATCGAT TCAAACATGT GATCTTCTAA GTGACCAATC AGAAGATGAA ACTACACCAG 
     3001 ATGAAGAAAT GTCTTCAAAC TTAGAGTATG CTAAAGGTTA CCCACCTTAT TCTCCATATA 
     3061 TAGGAAGTTC ACCCACTTTT TGTCATCTCC TTCATGAAAA AGTACCATTT TGCTGCTTAA 
     3121 GATTAGACAA GAGTTGCCAA CATAACTACT ATGAGGATGC AAAAGCCTAT GGATTCAAAA 
     3181 ATAAACTAAT TATAGTTGCA GCTGAAACAG CTGGAAATGG ATTATATAAC TTTATTGTTC 
     3241 CTCTCAGGGC ATATTATAGA CCAAAGAAAG AACTTAATCC CATAGTACTG CTATTGGATA 
     3301 ACCCGCCAGA TATGCATTTT CTGGATGCAA TCTGTTGGTT TCCAATGGTT TACTACATGG 
     3361 TGGGCTCTAT TGACAACCTA GATGACTTAC TCAGGTGTGG AGTGACTTTT GCTGCTAATA 
     3421 TGGTGGTTGT GGATAAAGAG AGCACCATGA GTGCCGAGGA AGACTACATG GCAGATGCCA 
     3481 AAACCATTGT GAACGTGCAG ACACTCTTCA GGTTGTTTTC CAGTCTCAGT ATTATCACAG 
     3541 AGCTAACTCA CCCCGCCAAC ATGAGATTCA TGCAATTCAG AGCCAAAGAC TGTTACTCTC 
     3601 TTGCTCTTTC AAAACTGGAA AAGAAAGAAC GGGAGAGAGG CTCTAACTTG GCCTTTATGT 
     3661 TTCGACTGCC TTTTGCTGCT GGGAGGGTGT TTAGCATCAG TATGTTGGAC ACTCTGCTGT 
     3721 ATCAGTCATT TGTGAAGGAT TATATGATTT CTATCACGAG ACTTCTGTTG GGACTGGACA 
     3781 CTACACCAGG ATCTGGGTTT CTTTGTTCTA TGAAAATCAC TGCAGATGAC TTATGGATCA 
     3841 GAACTTATGC CAGACTTTAT CAGAAGTTGT GTTCTTCTAC TGGAGATGTT CCCATTGGAA 
     3901 TCTACAGGAC TGAGTCTCAG AAACTTACTA CATCTGAGTC TCAAATATCT ATCAGTGTAG 
     3961 AAGAGTGGGA AGACACCAAA GACTCCAAAG AACAAGGGCA CCACCGCAGC AACCACCGCA 
     4021 ACTCAACATC CAGTGACCAG TCGGACCATC CCTTGCTGCG GAGAAAAAGC ATGCAGTGGG 
     4081 CCCGAAGACT GAGCAGAAAA GGCCCAAAAC ACTCTGGTAA AACAGCTGAA AAAATAACCC 
     4141 AGCAGCGACT GAACCTCTAC AGGAGGTCAG AAAGACAAGA GCTTGCTGAA CTTGTGAAAA 
     4201 ATAGAATGAA ACACTTGGGT CTTTCTACAG TGGGATATGA TGAAATGAAT GATCATCAAA 
     4261 GTACCCTCTC CTACATCCTG ATTAACCCAT CTCCAGATAC CAGAATAGAG CTGAATGATG 
     4321 TTGTATACTT AATTCGACCA GATCCACTGG CCTACCTTCC AAACAGTGAG CCCAGTCGAA 
     4381 GAAACAGCAT CTGCAATGTC ACTGGTCAAG ATTCTCGGGA GGAAACTCAA CTTACGCGTA 
     4441 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     4501 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4561 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4621 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4681 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     4741 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     4801 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     4861 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     4921 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     4981 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     5041 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     5101 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5161 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     5221 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     5281 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     5341 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     5401 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     5461 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     5521 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     5581 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     5641 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     5701 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     5761 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     5821 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     5881 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     5941 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     6001 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     6061 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     6121 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     6181 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     6241 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     6301 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     6361 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     6421 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     6481 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     6541 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     6601 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     6661 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     6721 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     6781 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     6841 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     6901 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     6961 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     7021 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     7081 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     7141 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     7201 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     7261 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     7321 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     7381 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     7441 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     7501 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     7561 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     7621 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7681 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     7741 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     7801 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     7861 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     7921 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     7981 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     8041 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     8101 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     8161 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     8221 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     8281 AATTTT
//