LOCUS       RC216220                9390 bp    DNA     circular  SYN 
DEFINITION  TRPM2 (Myc-DDK-tagged)-Human transient receptor potential cation channel, subfamily M, member 2 (TRPM2), transcript variant 1
ACCESSION   NM_003307
VERSION     NM_003307.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5547..5638 
                     /label="Myc-DDK"  
     misc_feature    5668..6253 
                     /label="PolyA signal"  
     misc_feature    5725..5744 
                     /label="Restriction Enzyme"  
     misc_feature    6529..7201 
                     /label="ColE1"  
     misc_feature    7631..8425 
                     /label="Kan/Neo"  
     misc_feature    8460..8762 
                     /label="SV40 ori"  
     misc_feature    8848..9325 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5529..5535 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5529 
                     /label="TRPM2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCCCTCA GCCCTGAGGA AAGCTGGCTC GGAGCAGGAG GAGGGCTTTG 
     1081 AGGGGCTGCC CAGAAGGGTC ACTGACCTGG GGATGGTCTC CAATCTCCGG CGCAGCAACA 
     1141 GCAGCCTCTT CAAGAGCTGG AGGCTACAGT GCCCCTTCGG CAACAATGAC AAGCAAGAAA 
     1201 GCCTCAGTTC GTGGATTCCT GAAAACATCA AGAAGAAAGA ATGCGTGTAT TTTGTGGAAA 
     1261 GTTCCAAACT GTCTGATGCT GGGAAGGTGG TGTGTCAGTG TGGCTACACG CATGAGCAGC 
     1321 ACTTGGAGGA GGCTACCAAG CCCCACACCT TCCAGGGCAC ACAGTGGGAC CCAAAGAAAC 
     1381 ATGTCCAGGA GATGCCAACC GATGCCTTTG GCGACATCGT CTTCACGGGC CTGAGCCAGA 
     1441 AGGTGAAAAA GTACGTCCGA GTCTCCCAGG ACACGCCCTC CAGCGTGATC TACCACCTCA 
     1501 TGACCCAGCA CTGGGGGCTG GACGTCCCCA ATCTCTTGAT CTCGGTGACC GGGGGGGCCA 
     1561 AGAACTTCAA CATGAAGCCG CGGCTGAAGA GCATTTTCCG CAGAGGCCTG GTCAAGGTGG 
     1621 CTCAGACCAC AGGGGCCTGG ATCATCACAG GGGGGTCCCA CACCGGCGTC ATGAAGCAGG 
     1681 TAGGCGAGGC GGTGCGGGAC TTCAGCCTGA GCAGCAGCTA CAAGGAAGGC GAGCTCATCA 
     1741 CCATCGGAGT CGCCACCTGG GGCACTGTCC ACCGCCGCGA GGGCCTGATC CATCCCACGG 
     1801 GCAGCTTCCC CGCCGAGTAC ATACTGGATG AGGATGGCCA AGGGAACCTG ACCTGCCTAG 
     1861 ACAGCAACCA CTCTCACTTC ATCCTCGTGG ACGACGGGAC CCACGGCCAG TACGGGGTGG 
     1921 AGATTCCTCT GAGGACCAGG CTGGAGAAGT TCATATCGGA GCAGACCAAG GAAAGAGGAG 
     1981 GTGTGGCCAT CAAGATCCCC ATCGTGTGCG TGGTGCTGGA GGGCGGCCCG GGCACGTTGC 
     2041 ACACCATCGA CAACGCCACC ACCAACGGCA CCCCCTGTGT GGTTGTGGAG GGCTCGGGCC 
     2101 GCGTGGCCGA CGTCATTGCC CAGGTGGCCA ACCTGCCTGT CTCGGACATC ACTATCTCCC 
     2161 TGATCCAGCA GAAACTGAGC GTGTTCTTCC AGGAGATGTT TGAGACCTTC ACGGAAAGCA 
     2221 GGATTGTCGA GTGGACCAAA AAGATCCAAG ATATCGTCCG GAGGCGGCAG CTGCTGACTG 
     2281 TCTTCCGGGA AGGCAAGGAT GGTCAGCAGG ACGTGGATGT GGCCATCTTG CAGGCCTTGC 
     2341 TGAAAGCCTC ACGGAGCCAA GACCACTTTG GCCACGAGAA CTGGGACCAC CAGCTGAAAC 
     2401 TGGCAGTGGC ATGGAATCGC GTGGACATTG CCCGCAGTGA GATCTTCATG GATGAGTGGC 
     2461 AGTGGAAGCC TTCAGATCTG CACCCCACGA TGACAGCTGC ACTCATCTCC AACAAGCCTG 
     2521 AGTTTGTGAA GCTCTTCCTG GAGAACGGGG TGCAGCTGAA GGAGTTTGTC ACCTGGGACA 
     2581 CCTTGCTCTA CCTGTACGAG AACCTGGACC CCTCCTGCCT GTTCCACAGC AAGCTGCAGA 
     2641 AGGTGCTGGT GGAGGATCCC GAGCGCCCGG CTTGCGCGCC CGCGGCGCCC CGCCTGCAGA 
     2701 TGCACCACGT GGCCCAGGTG CTGCGGGAGC TGCTGGGGGA CTTCACGCAG CCGCTTTATC 
     2761 CCCGGCCCCG GCACAACGAC CGGCTGCGGC TCCTGCTGCC CGTTCCCCAC GTCAAGCTCA 
     2821 ACGTGCAGGG AGTGAGCCTC CGGTCCCTCT ACAAGCGTTC CTCAGGCCAT GTGACCTTCA 
     2881 CCATGGACCC CATCCGTGAC CTTCTCATTT GGGCCATTGT CCAGAACCGT CGGGAGCTGG 
     2941 CAGGAATCAT CTGGGCTCAG AGCCAGGACT GCATCGCAGC GGCCTTGGCC TGCAGCAAGA 
     3001 TCCTGAAGGA ACTGTCCAAG GAGGAGGAGG ACACGGACAG CTCGGAGGAG ATGCTGGCGC 
     3061 TGGCGGAGGA GTATGAGCAC AGAGCCATCG GGGTCTTCAC CGAGTGCTAC CGGAAGGACG 
     3121 AAGAGAGAGC CCAGAAACTG CTCACCCGCG TGTCCGAGGC CTGGGGGAAG ACCACCTGCC 
     3181 TGCAGCTCGC CCTGGAGGCC AAGGACATGA AGTTTGTGTC TCACGGGGGC ATCCAGGCCT 
     3241 TCCTGACCAA GGTGTGGTGG GGCCAGCTCT CCGTGGACAA TGGGCTGTGG CGTGTGACCC 
     3301 TGTGCATGCT GGCCTTCCCG CTGCTCCTCA CCGGCCTCAT CTCCTTCAGG GAGAAGAGGC 
     3361 TGCAGGATGT GGGCACCCCC GCGGCCCGCG CCCGTGCCTT CTTCACCGCA CCCGTGGTGG 
     3421 TCTTCCACCT GAACATCCTC TCCTACTTCG CCTTCCTCTG CCTGTTCGCC TACGTGCTCA 
     3481 TGGTGGACTT CCAGCCTGTG CCCTCCTGGT GCGAGTGTGC CATCTACCTC TGGCTCTTCT 
     3541 CCTTGGTGTG CGAGGAGATG CGGCAGCTCT TCTATGACCC TGACGAGTGC GGGCTGATGA 
     3601 AGAAGGCAGC CTTGTACTTC AGTGACTTCT GGAATAAGCT GGACGTCGGC GCAATCTTGC 
     3661 TCTTCGTGGC AGGGCTGACC TGCAGGCTCA TCCCGGCGAC GCTGTACCCC GGGCGCGTCA 
     3721 TCCTCTCTCT GGACTTCATC CTGTTCTGCC TCCGGCTCAT GCACATTTTT ACCATCAGTA 
     3781 AGACGCTGGG GCCCAAGATC ATCATTGTGA AGCGGATGAT GAAGGACGTC TTCTTCTTCC 
     3841 TCTTCCTGCT GGCTGTGTGG GTGGTGTCCT TCGGGGTGGC CAAGCAGGCC ATCCTCATCC 
     3901 ACAACGAGCG CCGGGTGGAC TGGCTGTTCC GAGGGGCCGT CTACCACTCC TACCTCACCA 
     3961 TCTTCGGGCA GATCCCGGGC TACATCGACG GTGTGAACTT CAACCCGGAG CACTGCAGCC 
     4021 CCAATGGCAC CGACCCCTAC AAGCCTAAGT GCCCCGAGAG CGACGCGACG CAGCAGAGGC 
     4081 CGGCCTTCCC TGAGTGGCTG ACGGTCCTCC TACTCTGCCT CTACCTGCTC TTCACCAACA 
     4141 TCCTGCTGCT CAACCTCCTC ATCGCCATGT TCAACTACAC CTTCCAGCAG GTGCAGGAGC 
     4201 ACACGGACCA GATTTGGAAG TTCCAGCGCC ATGACCTGAT CGAGGAGTAC CACGGCCGCC 
     4261 CCGCCGCGCC GCCCCCCTTC ATCCTCCTCA GCCACCTGCA GCTCTTCATC AAGAGGGTGG 
     4321 TCCTGAAGAC TCCGGCCAAG AGGCACAAGC AGCTCAAGAA CAAGCTGGAG AAGAACGAGG 
     4381 AGGCGGCCCT GCTATCCTGG GAGATCTACC TGAAGGAGAA CTACCTCCAG AACCGACAGT 
     4441 TCCAGCAAAA GCAGCGGCCC GAGCAGAAGA TCGAGGACAT CAGCAATAAG GTTGACGCCA 
     4501 TGGTGGACCT GCTGGACCTG GACCCACTGA AGAGGTCGGG CTCCATGGAG CAGAGGTTGG 
     4561 CCTCCCTGGA GGAGCAGGTG GCCCAGACAG CCCGAGCCCT GCACTGGATC GTGAGGACGC 
     4621 TGCGGGCCAG CGGCTTCAGC TCGGAGGCGG ACGTCCCCAC TCTGGCCTCC CAGAAGGCCG 
     4681 CGGAGGAGCC GGATGCTGAG CCGGGAGGCA GGAAGAAGAC GGAGGAGCCG GGCGACAGCT 
     4741 ACCACGTGAA TGCCCGGCAC CTCCTCTACC CCAACTGCCC TGTCACGCGC TTCCCCGTGC 
     4801 CCAACGAGAA GGTGCCCTGG GAGACGGAGT TCCTGATCTA TGACCCACCC TTTTACACGG 
     4861 CAGAGAGGAA GGACGCGGCC GCCATGGACC CCATGGGAGA CACCCTGGAG CCACTGTCCA 
     4921 CGATCCAGTA CAACGTGGTG GATGGCCTGA GGGACCGCCG GAGCTTCCAC GGGCCGTACA 
     4981 CAGTGCAGGC CGGGTTGCCC CTGAACCCCA TGGGCCGCAC AGGACTGCGT GGGCGCGGGA 
     5041 GCCTCAGCTG CTTCGGACCC AACCACACGC TGTACCCCAT GGTCACGCGG TGGAGGCGGA 
     5101 ACGAGGATGG AGCCATCTGC AGGAAGAGCA TAAAGAAGAT GCTGGAAGTG CTGGTGGTGA 
     5161 AGCTCCCTCT CTCCGAGCAC TGGGCCCTGC CTGGGGGCTC CCGGGAGCCA GGGGAGATGC 
     5221 TACCTCGGAA GCTGAAGCGG ATCCTCCGGC AGGAGCACTG GCCGTCTTTT GAAAACTTGC 
     5281 TGAAGTGCGG CATGGAGGTG TACAAAGGCT ACATGGATGA CCCGAGGAAC ACGGACAATG 
     5341 CCTGGATCGA GACGGTGGCC GTCAGCGTCC ACTTCCAGGA CCAGAATGAC GTGGAGCTGA 
     5401 ACAGGCTGAA CTCTAACCTG CACGCCTGCG ACTCGGGGGC CTCCATCCGA TGGCAGGTGG 
     5461 TGGACAGGCG CATCCCACTC TATGCGAACC ACAAGACCCT CCTCCAGAAG GCAGCCGCTG 
     5521 AGTTCGGGGC TCACTACACG CGTACGCGGC CGCTCGAGCA GAAACTCATC TCAGAAGAGG 
     5581 ATCTGGCAGC AAATGATATC CTGGATTACA AGGATGACGA CGATAAGGTT TAAACGGCCG 
     5641 GCCGCGGTCA TAGCTGTTTC CTGAACAGAT CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA 
     5701 GTGCCTCTCC TGGCCCTGGA AGTTGCCACT CCAGTGCCCA CCAGCCTTGT CCTAATAAAA 
     5761 TTAAGTTGCA TCATTTTGTC TGACTAGGTG TCCTTCTATA ATATTATGGG GTGGAGGGGG 
     5821 GTGGTATGGA GCAAGGGGCA AGTTGGGAAG ACAACCTGTA GGGCCTGCGG GGTCTATTGG 
     5881 GAACCAAGCT GGAGTGCAGT GGCACAATCT TGGCTCACTG CAATCTCCGC CTCCTGGGTT 
     5941 CAAGCGATTC TCCTGCCTCA GCCTCCCGAG TTGTTGGGAT TCCAGGCATG CATGACCAGG 
     6001 CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA CGGGGTTTCA CCATATTGGC CAGGCTGGTC 
     6061 TCCAACTCCT AATCTCAGGT GATCTACCCA CCTTGGCCTC CCAAATTGCT GGGATTACAG 
     6121 GCGTGAACCA CTGCTCCCTT CCCTGTCCTT CTGATTTTAA AATAACTATA CCAGCAGGAG 
     6181 GACGTCCAGA CACAGCATAG GCTACCTGGC CATGCCCAAC CGGTGGGACA TTTGAGTTGC 
     6241 TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG TCCACTCAGT AGATGCCTGT TGAATTGGGT 
     6301 ACGCGGCCAG CGGCGAGCGG TATCAGCTCA CTCAAAGGCG GTAATACGGT TATCCACAGA 
     6361 ATCAGGGGAT AACGCAGGAA AGAACATGTG AGCAAAAGGC CAGCAAAAGG CCAGGAACCG 
     6421 TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA TAGGCTCCGC CCCCCTGACG AGCATCACAA 
     6481 AAATCGACGC TCAAGTCAGA GGTGGCGAAA CCCGACAGGA CTATAAAGAT ACCAGGCGTT 
     6541 TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC TGTTCCGACC CTGCCGCTTA CCGGATACCT 
     6601 GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC GCTTTCTCAT AGCTCACGCT GTAGGTATCT 
     6661 CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT GGGCTGTGTG CACGAACCCC CCGTTCAGCC 
     6721 CGACCGCTGC GCCTTATCCG GTAACTATCG TCTTGAGTCC AACCCGGTAA GACACGACTT 
     6781 ATCGCCACTG GCAGCAGCCA CTGGTAACAG GATTAGCAGA GCGAGGTATG TAGGCGGTGC 
     6841 TACAGAGTTC TTGAAGTGGT GGCCTAACTA CGGCTACACT AGAAGAACAG TATTTGGTAT 
     6901 CTGCGCTCTG CTGAAGCCAG TTACCTTCGG AAAAAGAGTT GGTAGCTCTT GATCCGGCAA 
     6961 ACAAACCACC GCTGGTAGCG GTGGTTTTTT TGTTTGCAAG CAGCAGATTA CGCGCAGAAA 
     7021 AAAAGGATCT CAAGAAGATC CTTTGATCTT TTCTACGGGG TCTGACGCTC AGTGGAACGA 
     7081 AAACTCACGT TAAGGGATTT TGGTCATGAG ATTATCAAAA AGGATCTTCA CCTAGATCCT 
     7141 TTTAAATTAA AAATGAAGTT TTAAATCAAT CTAAAGTATA TATGAGTAAC CTGAGGCTAT 
     7201 GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC GAGCCCTGGG CCTTCACCCG AACTTGGGGG 
     7261 GTGGGGTGGG GAAAAGGAAG AAACGCGGGC GTATTGGCCC CAATGGGGTC TCGGTGGGGT 
     7321 ATCGACAGAG TGCCAGCCCT GGGACCGAAC CCCGCGTTTA TGAACAAACG ACCCAACACC 
     7381 GTGCGTTTTA TTCTGTCTTT TTATTGCCGT CATAGCGCGG GTTCCTTCCG GTATTGTCTC 
     7441 CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG GTGGGCGAAG AACTCCAGCA TGAGATCCCC 
     7501 GCGCTGGAGG ATCATCCAGC CGGCGTCCCG GAAAACGATT CCGAAGCCCA ACCTTTCATA 
     7561 GAAGGCGGCG GTGGAATCGA AATCTCGTGA TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT 
     7621 TTCGAACCCC AGAGTCCCGC TCAGAAGAAC TCGTCAAGAA GGCGATAGAA GGCGATGCGC 
     7681 TGCGAATCGG GAGCGGCGAT ACCGTAAAGC ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA 
     7741 AGCTCTTCAG CAATATCACG GGTAGCCAAC GCTATGTCCT GATAGCGATC CGCCACACCC 
     7801 AGCCGGCCAC AGTCGATGAA TCCAGAAAAG CGGCCATTTT CCACCATGAT ATTCGGCAAG 
     7861 CAGGCATCGC CATGGGTCAC GACGAGATCC TCGCCGTCGG GCATGCTCGC CTTGAGCCTG 
     7921 GCGAACAGTT CGGCTGGCGC GAGCCCCTGA TGCTCTTCGT CCAGATCATC CTGATCGACA 
     7981 AGACCGGCTT CCATCCGAGT ACGTGCTCGC TCGATGCGAT GTTTCGCTTG GTGGTCGAAT 
     8041 GGGCAGGTAG CCGGATCAAG CGTATGCAGC CGCCGCATTG CATCAGCCAT GATGGATACT 
     8101 TTCTCGGCAG GAGCAAGGTG AGATGACAGG AGATCCTGCC CCGGCACTTC GCCCAATAGC 
     8161 AGCCAGTCCC TTCCCGCTTC AGTGACAACG TCGAGCACAG CTGCGCAAGG AACGCCCGTC 
     8221 GTGGCCAGCC ACGATAGCCG CGCTGCCTCG TCTTGCAGTT CATTCAGGGC ACCGGACAGG 
     8281 TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC TGCGCTGACA GCCGGAACAC GGCGGCATCA 
     8341 GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA TAGCCGAATA GCCTCTCCAC CCAAGCGGCC 
     8401 GGAGAACCTG CGTGCAATCC ATCTTGTTCA ATCATGCGAA ACGATCCTCA TCCTGTCTCT 
     8461 TGATCGATCT TTGCAAAAGC CTAGGCCTCC AAAAAAGCCT CCTCACTACT TCTGGAATAG 
     8521 CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC ATAAATAAAA AAAATTAGTC AGCCATGGGG 
     8581 CGGAGAATGG GCGGAACTGG GCGGAGTTAG GGGCGGGATG GGCGGAGTTA GGGGCGGGAC 
     8641 TATGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     8701 GGACTTTCCA CACCTGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     8761 TGGGGAGCCT GGGGACTTTC CACACCCTAA CTGACACACA TTCCACAGCT GGTTCTTTCC 
     8821 GCCTCAGGAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     8881 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTTCC GCGCACATTT 
     8941 CCCCGAAAAG TGCCACCTGA CGCGCCCTGT AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG 
     9001 GTTACGCGCA GCGTGACCGC TACACTTGCC AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC 
     9061 TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC 
     9121 CCTTTAGGGT TCCGATTTAG TGCTTTACGG CACCTCGACC CCAAAAAACT TGATTAGGGT 
     9181 GATGGTTCAC GTAGTGGGCC ATCGCCCTGA TAGACGGTTT TTCGCCCTTT GACGTTGGAG 
     9241 TCCACGTTCT TTAATAGTGG ACTCTTGTTC CAAACTGGAA CAACACTCAA CCCTATCTCG 
     9301 GTCTATTCTT TTGATTTATA AGGGATTTTG CCGATTTCGG CCTATTGGTT AAAAAATGAG 
     9361 CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//