LOCUS       RC216150                8400 bp    DNA     circular  SYN 
DEFINITION  PLCB1 (Myc-DDK-tagged)-Human phospholipase C, beta 1 (phosphoinositide-specific) (PLCB1), transcript variant 2
ACCESSION   NM_182734
VERSION     NM_182734.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4557..4648 
                     /label="Myc-DDK"  
     misc_feature    4678..5263 
                     /label="PolyA signal"  
     misc_feature    4735..4754 
                     /label="Restriction Enzyme"  
     misc_feature    5539..6211 
                     /label="ColE1"  
     misc_feature    6641..7435 
                     /label="Kan/Neo"  
     misc_feature    7470..7772 
                     /label="SV40 ori"  
     misc_feature    7858..8335 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4539..4545 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4539 
                     /label="PLCB1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCGGGGCT CAACCCGGAG TGCACGCCTT GCAACTCAAG CCCGTGTGCG 
     1081 TGTCCGACAG CCTCAAGAAG GGCACCAAAT TCGTCAAGTG GGATGATGAC TCAACTATTG 
     1141 TTACTCCAAT TATTTTGAGG ACTGACCCTC AGGGATTTTT CTTTTACTGG ACAGATCAAA 
     1201 ACAAGGAGAC AGAGCTACTG GATCTCAGCC TTGTCAAAGA TGCCAGATGT GGGAGACACG 
     1261 CCAAAGCTCC CAAGGACCCC AAATTACGTG AACTTTTGGA TGTGGGGAAC ATCGGGCGCC 
     1321 TGGAGCAGCG CATGATCACA GTGGTGTATG GGCCTGACCT CGTGAACATC TCCCATTTGA 
     1381 ATCTCGTGGC TTTCCAAGAA GAAGTGGCCA AGGAATGGAC AAATGAGGTT TTCAGTTTGG 
     1441 CAACAAACCT GCTGGCCCAA AACATGTCCA GGGATGCATT TCTGGAAAAA GCCTATACTA 
     1501 AACTTAAGCT GCAAGTCACT CCAGAAGGGC GTATTCCTCT CAAAAACATA TATCGCTTGT 
     1561 TTTCAGCAGA TCGGAAGCGA GTTGAAACTG CTTTAGAGGC TTGTAGTCTT CCATCTTCAA 
     1621 GGAATGATTC AATACCTCAA GAAGATTTCA CTCCAGAAGT GTACAGAGTT TTCCTCAACA 
     1681 ACCTTTGCCC TCGACCTGAA ATTGATAACA TCTTTTCAGA ATTTGGTGCA AAAAGCAAAC 
     1741 CATATCTTAC CGTTGATCAG ATGATGGATT TTATCAACCT TAAGCAGCGA GATCCTCGGC 
     1801 TTAATGAAAT ACTTTATCCA CCTCTAAAAC AAGAGCAAGT CCAAGTATTG ATTGAGAAGT 
     1861 ATGAACCCAA CAACAGCCTC GCCAGAAAAG GACAAATATC AGTGGATGGG TTCATGCGCT 
     1921 ATCTGAGTGG AGAAGAAAAC GGAGTCGTTT CACCTGAGAA ACTGGATTTG AATGAAGACA 
     1981 TGTCTCAGCC CCTTTCTCAC TATTTCATTA ATTCCTCGCA CAACACCTAC CTCACAGCTG 
     2041 GCCAACTGGC TGGAAACTCC TCTGTTGAGA TGTATCGCCA AGTGCTCCTG TCTGGTTGTC 
     2101 GCTGTGTGGA GCTGGACTGC TGGAAGGGAC GGACTGCAGA AGAGGAACCT GTCATCACCC 
     2161 ATGGCTTCAC CATGACAACT GAAATATCTT TCAAGGAAGT GATAGAAGCA ATTGCGGAGT 
     2221 GTGCATTTAA GACTTCACCT TTTCCAATTC TCCTTTCGTT TGAGAACCAT GTGGATTCCC 
     2281 CAAAGCAGCA AGCCAAGATG GCGGAGTACT GCCGACTGAT CTTTGGGGAT GCCCTTCTCA 
     2341 TGGAGCCCCT GGAAAAATAT CCACTGGAAT CTGGAGTTCC TCTTCCAAGC CCTATGGATT 
     2401 TAATGTATAA AATTTTGGTG AAAAATAAGA AGAAATCACA CAAGTCATCA GAAGGAAGCG 
     2461 GCAAAAAGAA GCTCTCAGAA CAAGCCTCCA ACACCTACAG TGACTCCTCC AGCATGTTCG 
     2521 AGCCCTCATC CCCAGGAGCC GGAGAAGCTG ATACGGAAAG TGACGACGAC GATGATGATG 
     2581 ATGACTGTAA AAAATCTTCA ATGGATGAGG GGACTGCTGG AAGTGAGGCT ATGGCCACAG 
     2641 AAGAAATGTC TAATCTGGTG AACTATATTC AGCCAGTCAA GTTTGAGTCA TTTGAAATTT 
     2701 CAAAAAAAAG AAATAAAAGT TTTGAAATGT CTTCCTTCGT GGAAACCAAA GGACTTGAAC 
     2761 AACTCACCAA GTCTCCAGTG GAATTTGTAG AATATAACAA AATGCAGCTT AGCAGGATAT 
     2821 ATCCAAAAGG AACACGTGTG GATTCATCCA ACTATATGCC TCAGCTCTTC TGGAATGCAG 
     2881 GTTGTCAGAT GGTGGCACTT AATTTCCAGA CAATGGACCT GGCTATGCAA ATAAATATGG 
     2941 GGATGTATGA ATACAACGGG AAGAGTGGCT ACAGATTGAA GCCAGAGTTC ATGAGGAGGC 
     3001 CTGACAAGCA TTTTGATCCA TTTACTGAAG GCATCGTAGA TGGGATAGTG GCAAACACTT 
     3061 TGTCTGTTAA GATTATTTCA GGTCAGTTTC TTTCTGATAA GAAAGTTGGG ACTTACGTGG 
     3121 AAGTAGATAT GTTTGGTTTG CCTGTGGATA CAAGGAGGAA GGCATTTAAG ACCAAAACAT 
     3181 CCCAAGGAAA TGCTGTGAAT CCTGTCTGGG AAGAAGAACC TATTGTGTTC AAAAAGGTGG 
     3241 TTCTTCCTAC TCTGGCCTGT TTGAGAATAG CAGTTTATGA AGAAGGAGGT AAATTCATTG 
     3301 GCCACCGTAT CTTGCCAGTG CAAGCCATTC GGCCAGGCTA TCACTATATC TGTCTAAGGA 
     3361 ATGAAAGGAA CCAGCCTCTG ACGCTGCCTG CTGTCTTTGT CTACATAGAA GTGAAAGACT 
     3421 ATGTGCCAGA CACATATGCA GATGTCATCG AAGCTTTATC AAACCCAATC CGATATGTGA 
     3481 ACCTGATGGA ACAGAGAGCT AAGCAATTGG CTGCTTTGAC ACTGGAAGAT GAAGAAGAAG 
     3541 TAAAGAAAGA GGCTGATCCT GGAGAAACAC CATCAGAGGC TCCAAGTGAA GCGAGAACGA 
     3601 CTCCAGCAGA AAATGGGGTG AATCACACTA CAACCCTGAC ACCCAAGCCA CCCTCCCAGG 
     3661 CTCTCCACAG CCAGCCAGCT CCAGGTTCTG TAAAGGCACC TGCCAAAACA GAAGATCTTA 
     3721 TTCAGAGTGT CTTAACAGAA GTGGAAGCAC AGACCATCGA AGAACTAAAG CAACAGAAAT 
     3781 CGTTTGTGAA ACTTCAAAAG AAACACTACA AAGAAATGAA AGACCTGGTT AAGAGACACC 
     3841 ACAAGAAAAC CACTGACCTT ATCAAAGAAC ACACTACCAA GTATAATGAA ATTCAGAATG 
     3901 ACTACTTGAG AAGGAGAGCC GCTTTGGAAA AGTCCGCCAA AAAGGACAGT AAGAAAAAAT 
     3961 CGGAACCCAG CAGCCCTGAT CATGGTTCAT CAACGATTGA GCAAGACCTC GCTGCTCTGG 
     4021 ATGCTGAAAT GACCCAAAAG TTAATAGACT TGAAGGACAA ACAACAGCAG CAGCTGCTTA 
     4081 ATCTTCGGCA AGAACAGTAT TATAGTGAAA AATACCAGAA GCGAGAACAT ATTAAACTGC 
     4141 TTATTCAAAA GTTGACGGAT GTCGCAGAAG AGTGTCAGAA CAATCAGTTA AAGAAGCTCA 
     4201 AAGAAATCTG TGAGAAAGAA AAGAAAGAAT TAAAGAAGAA AATGGATAAA AAGAGGCAGG 
     4261 AGAAGATAAC AGAAGCTAAA TCCAAAGACA AAAGTCAGAT GGAAGAGGAG AAGACAGAGA 
     4321 TGATCCGGTC ATATATCCAG GAAGTGGTGC AGTATATCAA GAGGCTAGAA GAAGCGCAAA 
     4381 GTAAACGGCA AGAAAAACTC GTAGAGAAAC ACAAGGAAAT ACGTCAGCAG ATCCTGGATG 
     4441 AAAAGCCCAA GGGGGAAGGT TCCTCCTCAT TCTTGTCGGA AACTTGCCAT GAGGATCCCT 
     4501 CTGTTTCCCC CAACTTTACT CCCCCCAACC CTCAAGCTCT CAAGTGGACG CGTACGCGGC 
     4561 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     4621 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     4681 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     4741 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     4801 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     4861 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     4921 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     4981 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     5041 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     5101 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     5161 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     5221 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     5281 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     5341 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     5401 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     5461 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     5521 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     5581 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     5641 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     5701 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     5761 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     5821 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     5881 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     5941 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     6001 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     6061 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     6121 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     6181 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     6241 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     6301 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     6361 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     6421 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     6481 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     6541 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     6601 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     6661 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     6721 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     6781 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     6841 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     6901 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     6961 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     7021 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     7081 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     7141 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     7201 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     7261 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     7321 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     7381 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     7441 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     7501 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     7561 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     7621 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     7681 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     7741 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     7801 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     7861 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     7921 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     7981 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     8041 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     8101 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     8161 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     8221 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     8281 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     8341 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//