LOCUS       RC216106L3                9085 bp    DNA     circular  SYN 
DEFINITION  GLB1 (Myc-DDK-tagged)-Human galactosidase, beta 1 (GLB1), transcript variant 1
ACCESSION   NM_000404
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    7376..7556 
                     /label="3-LTR"  
     misc_feature    833..1013 
                     /label="5-LTR"  
     misc_feature    8284..8943 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV"  
     misc_feature    7604..8223 
                     /label="ColE1"  
     misc_feature    5416..5439 
                     /label="DDK"  
     misc_feature    6251..6271 
                     /label="LR50"  
     misc_feature    5368..5439 
                     /label="MYC"  
     misc_feature    5449..5514 
                     /label="P2A"  
     misc_feature    5515..6114 
                     /label="Puro"  
     misc_feature    3252..3271 
                     /label="T7"  
     misc_feature    3221..3237 
                     /label="V2"  
     misc_feature    6260..6851 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5350..5356 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5350 
                     /label="GLB1"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GCCGGGGTTC CTGGTTCGCA TCCTCCCTCT 
     3361 GCTGCTGGTT CTGCTGCTTC TGGGCCCTAC GCGCGGCTTG CGCAATGCCA CCCAGAGGAT 
     3421 GTTTGAAATT GACTATAGCC GGGACTCCTT CCTCAAGGAT GGCCAGCCAT TTCGCTACAT 
     3481 CTCAGGAAGC ATTCACTACT CCCGTGTGCC CCGCTTCTAC TGGAAGGACC GGCTGCTGAA 
     3541 GATGAAGATG GCTGGGCTGA ACGCCATCCA GACGTATGTG CCCTGGAACT TTCATGAGCC 
     3601 CTGGCCAGGA CAGTACCAGT TTTCTGAGGA CCATGATGTG GAATATTTTC TTCGGCTGGC 
     3661 TCATGAGCTG GGACTGCTGG TTATCCTGAG GCCCGGGCCC TACATCTGTG CAGAGTGGGA 
     3721 AATGGGAGGA TTACCTGCTT GGCTGCTAGA GAAAGAGTCT ATTCTTCTCC GCTCCTCCGA 
     3781 CCCAGATTAC CTGGCAGCTG TGGACAAGTG GTTGGGAGTC CTTCTGCCCA AGATGAAGCC 
     3841 TCTCCTCTAT CAGAATGGAG GGCCAGTTAT AACAGTGCAG GTTGAAAATG AATATGGCAG 
     3901 CTACTTTGCC TGTGATTTTG ACTACCTGCG CTTCCTGCAG AAGCGCTTTC GCCACCATCT 
     3961 GGGGGATGAT GTGGTTCTGT TTACCACTGA TGGAGCACAT AAAACATTCC TGAAATGTGG 
     4021 GGCCCTGCAG GGCCTCTACA CCACGGTGGA CTTTGGAACA GGCAGCAACA TCACAGATGC 
     4081 TTTCCTAAGC CAGAGGAAGT GTGAGCCCAA AGGACCCTTG ATCAATTCTG AATTCTATAC 
     4141 TGGCTGGCTA GATCACTGGG GCCAACCTCA CTCCACAATC AAGACCGAAG CAGTGGCTTC 
     4201 CTCCCTCTAT GATATACTTG CCCGTGGGGC GAGTGTGAAC TTGTACATGT TTATAGGTGG 
     4261 GACCAATTTT GCCTATTGGA ATGGGGCCAA CTCACCCTAT GCAGCACAGC CCACCAGCTA 
     4321 CGACTATGAT GCCCCACTGA GTGAGGCTGG GGACCTCACT GAGAAGTATT TTGCTCTGCG 
     4381 AAACATCATC CAGAAGTTTG AAAAAGTACC AGAAGGTCCT ATCCCTCCAT CTACACCAAA 
     4441 GTTTGCATAT GGAAAGGTCA CTTTGGAAAA GTTAAAGACA GTGGGAGCAG CTCTGGACAT 
     4501 TCTGTGTCCC TCTGGGCCCA TCAAAAGCCT TTATCCCTTG ACATTTATCC AGGTGAAACA 
     4561 GCATTATGGG TTTGTGCTGT ACCGGACAAC ACTTCCTCAA GATTGCAGCA ACCCAGCACC 
     4621 TCTCTCTTCA CCCCTCAATG GAGTCCACGA TCGAGCATAT GTTGCTGTGG ATGGGATCCC 
     4681 CCAGGGAGTC CTTGAGCGAA ACAATGTGAT CACTCTGAAC ATAACAGGGA AAGCTGGAGC 
     4741 CACTCTGGAC CTTCTGGTAG AGAACATGGG ACGTGTGAAC TATGGTGCAT ATATCAACGA 
     4801 TTTTAAGGGT TTGGTTTCTA ACCTGACTCT CAGTTCCAAT ATCCTCACGG ACTGGACGAT 
     4861 CTTTCCACTG GACACTGAGG ATGCAGTGCG CAGCCACCTG GGGGGCTGGG GACACCGTGA 
     4921 CAGTGGCCAC CATGATGAAG CCTGGGCCCA CAACTCATCC AACTACACGC TCCCGGCCTT 
     4981 TTATATGGGG AACTTCTCCA TTCCCAGTGG GATCCCAGAC TTGCCCCAGG ACACCTTTAT 
     5041 CCAGTTTCCT GGATGGACCA AGGGCCAGGT CTGGATTAAT GGCTTTAACC TTGGCCGCTA 
     5101 TTGGCCAGCC CGGGGCCCTC AGTTGACCTT GTTTGTGCCC CAGCACATCC TGATGACCTC 
     5161 GGCCCCAAAC ACCATCACCG TGCTGGAACT GGAGTGGGCA CCCTGCAGCA GTGATGATCC 
     5221 AGAACTATGT GCTGTGACGT TCGTGGACAG GCCAGTTATT GGCTCATCTG TGACCTACGA 
     5281 TCATCCCTCC AAACCTGTTG AAAAAAGACT CATGCCCCCA CCCCCGCAAA AAAACAAAGA 
     5341 TTCATGGCTG GACCATGTAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG 
     5401 GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTGGGTAGGA 
     5461 AGCGGAGCTA CTAACTTCAG CCTGCTGAAG CAGGCTGGAG ACGTGGAGGA GAACCCTGGA 
     5521 CCTATGACCG AGTACAAGCC CACGGTGCGC CTCGCCACCC GCGACGACGT CCCCAGGGCC 
     5581 GTACGCACCC TCGCCGCCGC GTTCGCCGAC TACCCCGCCA CGCGCCACAC CGTCGATCCG 
     5641 GATCGCCACA TCGAGCGGGT CACCGAGCTG CAAGAACTCT TCCTCACGCG CGTCGGGCTC 
     5701 GACATCGGCA AGGTGTGGGT CGCGGACGAC GGCGCCGCGG TGGCGGTCTG GACCACGCCG 
     5761 GAGAGCGTCG AAGCGGGGGC GGTGTTCGCC GAGATCGGCC CGCGCATGGC CGAGTTGAGC 
     5821 GGTTCCCGGC TGGCCGCGCA GCAACAGATG GAAGGCCTCC TGGCGCCGCA CCGGCCCAAG 
     5881 GAGCCCGCGT GGTTCCTGGC CACCGTCGGC GTCTCGCCCG ACCACCAGGG CAAGGGTCTG 
     5941 GGCAGCGCCG TCGTGCTCCC CGGAGTGGAG GCGGCCGAGC GCGCCGGGGT GCCCGCCTTC 
     6001 CTGGAGACCT CCGCGCCCCG CAACCTCCCC TTCTACGAGC GGCTCGGCTT CACCGTCACC 
     6061 GCCGACGTCG AGGTGCCCGA AGGACCGCGC ACCTGGTGCA TGACCCGCAA GCCCGGTGCC 
     6121 TGATGTACAC CCAAACGGCC GGCCGCGGTC TGTACAAGTA GGATTCGTCG AGGGACCTAA 
     6181 TAACTTCGTA TAGCATACAT TATACGAAGT TATACATGTT TAAGGGTTCC GGTTCCACTA 
     6241 GGTACAATTC GATATCAAGC TTATCGATAA TCAACCTCTG GATTACAAAA TTTGTGAAAG 
     6301 ATTGACTGGT ATTCTTAACT ATGTTGCTCC TTTTACGCTA TGTGGATACG CTGCTTTAAT 
     6361 GCCTTTGTAT CATGCTATTG CTTCCCGTAT GGCTTTCATT TTCTCCTCCT TGTATAAATC 
     6421 CTGGTTGCTG TCTCTTTATG AGGAGTTGTG GCCCGTTGTC AGGCAACGTG GCGTGGTGTG 
     6481 CACTGTGTTT GCTGACGCAA CCCCCACTGG TTGGGGCATT GCCACCACCT GTCAGCTCCT 
     6541 TTCCGGGACT TTCGCTTTCC CCCTCCCTAT TGCCACGGCG GAACTCATCG CCGCCTGCCT 
     6601 TGCCCGCTGC TGGACAGGGG CTCGGCTGTT GGGCACTGAC AATTCCGTGG TGTTGTCGGG 
     6661 GAAATCATCG TCCTTTCCTT GGCTGCTCGC CTGTGTTGCC ACCTGGATTC TGCGCGGGAC 
     6721 GTCCTTCTGC TACGTCCCTT CGGCCCTCAA TCCAGCGGAC CTTCCTTCCC GCGGCCTGCT 
     6781 GCCGGCTCTG CGGCCTCTTC CGCGTCTTCG CCTTCGCCCT CAGACGAGTC GGATCTCCCT 
     6841 TTGGGCCGCC TCCCCGCATC GATACCGTCG ACCTCGATCG AGACCTAGAA AAACATGGAG 
     6901 CAATCACAAG TAGCAATACA GCAGCTACCA ATGCTGATTG TGCCTGGCTA GAAGCACAAG 
     6961 AGGAGGAGGA GGTGGGTTTT CCAGTCACAC CTCAGGTACC TTTAAGACCA ATGACTTACA 
     7021 AGGCAGCTGT AGATCTTAGC CACTTTTTAA AAGAAAAGGG GGGACTGGAA GGGCTAATTC 
     7081 ACTCCCAACG AAGACAAGAT ATCCTTGATC TGTGGATCTA CCACACACAA GGCTACTTCC 
     7141 CTGATTGGCA GAACTACACA CCAGGGCCAG GGATCAGATA TCCACTGACC TTTGGATGGT 
     7201 GCTACAAGCT AGTACCAGTT GAGCAAGAGA AGGTAGAAGA AGCCAATGAA GGAGAGAACA 
     7261 CCCGCTTGTT ACACCCTGTG AGCCTGCATG GGATGGATGA CCCGGAGAGA GAAGTATTAG 
     7321 AGTGGAGGTT TGACAGCCGC CTAGCATTTC ATCACATGGC CCGAGAGCTG CATCCGGACT 
     7381 GTACTGGGTC TCTCTGGTTA GACCAGATCT GAGCCTGGGA GCTCTCTGGC TAACTAGGGA 
     7441 ACCCACTGCT TAAGCCTCAA TAAAGCTTGC CTTGAGTGCT TCAAGTAGTG TGTGCCCGTC 
     7501 TGTTGTGTGA CTCTGGTAAC TAGAGATCCC TCAGACCCTT TTAGTCAGTG TGGAAAATCT 
     7561 CTAGCAGCAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC 
     7621 TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC 
     7681 AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC 
     7741 TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT 
     7801 CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG 
     7861 TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT 
     7921 CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG 
     7981 CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT 
     8041 GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC 
     8101 CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA 
     8161 GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG 
     8221 ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA 
     8281 TTTTGGTCAT GATTACGCCC CGCCCTGCCA CTCATCGCAG TACTGTTGTA ATTCATTAAG 
     8341 CATTCTGCCG ACATGGAAGC CATCACAAAC GGCATGATGA ACCTGAATCG CCAGCGGCAT 
     8401 CAGCACCTTG TCGCCTTGCG TATAATATTT GCCCATGGTG AAAACGGGGG CGAAGAAGTT 
     8461 GTCCATATTG GCCACGTTTA AATCAAAACT GGTGAAACTC ACCCAGGGAT TGGCTGAGAC 
     8521 GAAAAACATA TTCTCAATAA ACCCTTTAGG GAAATAGGCC AGGTTTTCAC CGTAACACGC 
     8581 CACATCTTGC GAATATATGT GTAGAAACTG CCGGAAATCG TCGTGGTATT CACTCCAGAG 
     8641 CGATGAAAAC GTTTCAGTTT GCTCATGGAA AACGGTGTAA CAAGGGTGAA CACTATCCCA 
     8701 TATCACCAGC TCACCGTCTT TCATTGCCAT ACGGAACTCC GGATGAGCAT TCATCAGGCG 
     8761 GGCAAGAATG TGAATAAAGG CCGGATAAAA CTTGTGCTTA TTTTTCTTTA CGGTCTTTAA 
     8821 AAAGGCCGTA ATATCCAGCT GAACGGTCTG GTTATAGGTA CATTGAGCAA CTGACTGAAA 
     8881 TGCCTCAAAA TGTTCTTTAC GATGCCATTG GGATATATCA ACGGTGGTAT ATCCAGTGAT 
     8941 TTTTTTCTCC ATACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT 
     9001 CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC 
     9061 ATTTCCCCGA AAAGTGCCAC CTGAC
//