LOCUS       RC216080                8721 bp    DNA     circular  SYN 
DEFINITION  ABCB1 (Myc-DDK-tagged)-Human ATP-binding cassette, sub-family B (MDR/TAP), member 1 (ABCB1)
ACCESSION   NM_000927
VERSION     NM_000927.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4878..4969 
                     /label="Myc-DDK"  
     misc_feature    4999..5584 
                     /label="PolyA signal"  
     misc_feature    5056..5075 
                     /label="Restriction Enzyme"  
     misc_feature    5860..6532 
                     /label="ColE1"  
     misc_feature    6962..7756 
                     /label="Kan/Neo"  
     misc_feature    7791..8093 
                     /label="SV40 ori"  
     misc_feature    8179..8656 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4860..4866 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4860 
                     /label="ABCB1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATCTTGAA GGGGACCGCA ATGGAGGAGC AAAGAAGAAG AACTTTTTTA 
     1081 AACTGAACAA TAAAAGTGAA AAAGATAAGA AGGAAAAGAA ACCAACTGTC AGTGTATTTT 
     1141 CAATGTTTCG CTATTCAAAT TGGCTTGACA AGTTGTATAT GGTGGTGGGA ACTTTGGCTG 
     1201 CCATCATCCA TGGGGCTGGA CTTCCTCTCA TGATGCTGGT GTTTGGAGAA ATGACAGATA 
     1261 TCTTTGCAAA TGCAGGAAAT TTAGAAGATC TGATGTCAAA CATCACTAAT AGAAGTGATA 
     1321 TCAATGATAC AGGGTTCTTC ATGAATCTGG AGGAAGACAT GACCAGGTAT GCCTATTATT 
     1381 ACAGTGGAAT TGGTGCTGGG GTGCTGGTTG CTGCTTACAT TCAGGTTTCA TTTTGGTGCC 
     1441 TGGCAGCTGG AAGACAAATA CACAAAATTA GAAAACAGTT TTTTCATGCT ATAATGCGAC 
     1501 AGGAGATAGG CTGGTTTGAT GTGCACGATG TTGGGGAGCT TAACACCCGA CTTACAGATG 
     1561 ATGTCTCCAA GATTAATGAA GGAATTGGTG ACAAAATTGG AATGTTCTTT CAGTCAATGG 
     1621 CAACATTTTT CACTGGGTTT ATAGTAGGAT TTACACGTGG TTGGAAGCTA ACCCTTGTGA 
     1681 TTTTGGCCAT CAGTCCTGTT CTTGGACTGT CAGCTGCTGT CTGGGCAAAG ATACTATCTT 
     1741 CATTTACTGA TAAAGAACTC TTAGCGTATG CAAAAGCTGG AGCAGTAGCT GAAGAGGTCT 
     1801 TGGCAGCAAT TAGAACTGTG ATTGCATTTG GAGGACAAAA GAAAGAACTT GAAAGGTACA 
     1861 ACAAAAATTT AGAAGAAGCT AAAAGAATTG GGATAAAGAA AGCTATTACA GCCAATATTT 
     1921 CTATAGGTGC TGCTTTCCTG CTGATCTATG CATCTTATGC TCTGGCCTTC TGGTATGGGA 
     1981 CCACCTTGGT CCTCTCAGGG GAATATTCTA TTGGACAAGT ACTCACTGTA TTCTTTTCTG 
     2041 TATTAATTGG GGCTTTTAGT GTTGGACAGG CATCTCCAAG CATTGAAGCA TTTGCAAATG 
     2101 CAAGAGGAGC AGCTTATGAA ATCTTCAAGA TAATTGATAA TAAGCCAAGT ATTGACAGCT 
     2161 ATTCGAAGAG TGGGCACAAA CCAGATAATA TTAAGGGAAA TTTGGAATTC AGAAATGTTC 
     2221 ACTTCAGTTA CCCATCTCGA AAAGAAGTTA AGATCTTGAA GGGTCTGAAC CTGAAGGTGC 
     2281 AGAGTGGGCA GACGGTGGCC CTGGTTGGAA ACAGTGGCTG TGGGAAGAGC ACAACAGTCC 
     2341 AGCTGATGCA GAGGCTCTAT GACCCCACAG AGGGGATGGT CAGTGTTGAT GGACAGGATA 
     2401 TTAGGACCAT AAATGTAAGG TTTCTACGGG AAATCATTGG TGTGGTGAGT CAGGAACCTG 
     2461 TATTGTTTGC CACCACGATA GCTGAAAACA TTCGCTATGG CCGTGAAAAT GTCACCATGG 
     2521 ATGAGATTGA GAAAGCTGTC AAGGAAGCCA ATGCCTATGA CTTTATCATG AAACTGCCTC 
     2581 ATAAATTTGA CACCCTGGTT GGAGAGAGAG GGGCCCAGTT GAGTGGTGGG CAGAAGCAGA 
     2641 GGATCGCCAT TGCACGTGCC CTGGTTCGCA ACCCCAAGAT CCTCCTGCTG GATGAGGCCA 
     2701 CGTCAGCCTT GGACACAGAA AGCGAAGCAG TGGTTCAGGT GGCTCTGGAT AAGGCCAGAA 
     2761 AAGGTCGGAC CACCATTGTG ATAGCTCATC GTTTGTCTAC AGTTCGTAAT GCTGACGTCA 
     2821 TCGCTGGTTT CGATGATGGA GTCATTGTGG AGAAAGGAAA TCATGATGAA CTCATGAAAG 
     2881 AGAAAGGCAT TTACTTCAAA CTTGTCACAA TGCAGACAGC AGGAAATGAA GTTGAATTAG 
     2941 AAAATGCAGC TGATGAATCC AAAAGTGAAA TTGATGCCTT GGAAATGTCT TCAAATGATT 
     3001 CAAGATCCAG TCTAATAAGA AAAAGATCAA CTCGTAGGAG TGTCCGTGGA TCACAAGCCC 
     3061 AAGACAGAAA GCTTAGTACC AAAGAGGCTC TGGATGAAAG TATACCTCCA GTTTCCTTTT 
     3121 GGAGGATTAT GAAGCTAAAT TTAACTGAAT GGCCTTATTT TGTTGTTGGT GTATTTTGTG 
     3181 CCATTATAAA TGGAGGCCTG CAACCAGCAT TTGCAATAAT ATTTTCAAAG ATTATAGGGG 
     3241 TTTTTACAAG AATTGATGAT CCTGAAACAA AACGACAGAA TAGTAACTTG TTTTCACTAT 
     3301 TGTTTCTAGC CCTTGGAATT ATTTCTTTTA TTACATTTTT CCTTCAGGGT TTCACATTTG 
     3361 GCAAAGCTGG AGAGATCCTC ACCAAGCGGC TCCGATACAT GGTTTTCCGA TCCATGCTCA 
     3421 GACAGGATGT GAGTTGGTTT GATGACCCTA AAAACACCAC TGGAGCATTG ACTACCAGGC 
     3481 TCGCCAATGA TGCTGCTCAA GTTAAAGGGG CTATAGGTTC CAGGCTTGCT GTAATTACCC 
     3541 AGAATATAGC AAATCTTGGG ACAGGAATAA TTATATCCTT CATCTATGGT TGGCAACTAA 
     3601 CACTGTTACT CTTAGCAATT GTACCCATCA TTGCAATAGC AGGAGTTGTT GAAATGAAAA 
     3661 TGTTGTCTGG ACAAGCACTG AAAGATAAGA AAGAACTAGA AGGTTCTGGG AAGATCGCTA 
     3721 CTGAAGCAAT AGAAAACTTC CGAACCGTTG TTTCTTTGAC TCAGGAGCAG AAGTTTGAAC 
     3781 ATATGTATGC TCAGAGTTTG CAGGTACCAT ACAGAAACTC TTTGAGGAAA GCACACATCT 
     3841 TTGGAATTAC ATTTTCCTTC ACCCAGGCAA TGATGTATTT TTCCTATGCT GGATGTTTCC 
     3901 GGTTTGGAGC CTACTTGGTG GCACATAAAC TCATGAGCTT TGAGGATGTT CTGTTAGTAT 
     3961 TTTCAGCTGT TGTCTTTGGT GCCATGGCCG TGGGGCAAGT CAGTTCATTT GCTCCTGACT 
     4021 ATGCCAAAGC CAAAATATCA GCAGCCCACA TCATCATGAT CATTGAAAAA ACCCCTTTGA 
     4081 TTGACAGCTA CAGCACGGAA GGCCTAATGC CGAACACATT GGAAGGAAAT GTCACATTTG 
     4141 GTGAAGTTGT ATTCAACTAT CCCACCCGAC CGGACATCCC AGTGCTTCAG GGACTGAGCC 
     4201 TGGAGGTGAA GAAGGGCCAG ACGCTGGCTC TGGTGGGCAG CAGTGGCTGT GGGAAGAGCA 
     4261 CAGTGGTCCA GCTCCTGGAG CGGTTCTACG ACCCCTTGGC AGGGAAAGTG CTGCTTGATG 
     4321 GCAAAGAAAT AAAGCGACTG AATGTTCAGT GGCTCCGAGC ACACCTGGGC ATCGTGTCCC 
     4381 AGGAGCCCAT CCTGTTTGAC TGCAGCATTG CTGAGAACAT TGCCTATGGA GACAACAGCC 
     4441 GGGTGGTGTC ACAGGAAGAG ATTGTGAGGG CAGCAAAGGA GGCCAACATA CATGCCTTCA 
     4501 TCGAGTCACT GCCTAATAAA TATAGCACTA AAGTAGGAGA CAAAGGAACT CAGCTCTCTG 
     4561 GTGGCCAGAA ACAACGCATT GCCATAGCTC GTGCCCTTGT TAGACAGCCT CATATTTTGC 
     4621 TTTTGGATGA AGCCACGTCA GCTCTGGATA CAGAAAGTGA AAAGGTTGTC CAAGAAGCCC 
     4681 TGGACAAAGC CAGAGAAGGC CGCACCTGCA TTGTGATTGC TCACCGCCTG TCCACCATCC 
     4741 AGAATGCAGA CTTAATAGTG GTGTTTCAGA ATGGCAGAGT CAAGGAGCAT GGCACGCATC 
     4801 AGCAGCTGCT GGCACAGAAA GGCATCTATT TTTCAATGGT CAGTGTCCAG GCTGGAACAA 
     4861 AGCGCCAGAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     4921 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     4981 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     5041 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     5101 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     5161 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     5221 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     5281 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     5341 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     5401 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     5461 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     5521 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5581 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5641 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     5701 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     5761 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     5821 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     5881 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     5941 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     6001 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     6061 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     6121 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     6181 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     6241 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     6301 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     6361 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     6421 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     6481 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     6541 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     6601 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     6661 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     6721 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     6781 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     6841 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     6901 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     6961 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     7021 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     7081 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     7141 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     7201 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     7261 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     7321 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     7381 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     7441 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     7501 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     7561 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     7621 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     7681 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     7741 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     7801 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     7861 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     7921 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     7981 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8041 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     8101 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     8161 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     8221 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     8281 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     8341 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     8401 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     8461 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     8521 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     8581 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     8641 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     8701 CAAAAATTTA ACGCGAATTT T
//