LOCUS       RC216078                8823 bp    DNA     circular  SYN 
DEFINITION  MYO3B (Myc-DDK-tagged)-Human myosin IIIB (MYO3B), transcript variant 1
ACCESSION   NM_001083615
VERSION     NM_001083615.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4980..5071 
                     /label="Myc-DDK"  
     misc_feature    5101..5686 
                     /label="PolyA signal"  
     misc_feature    5158..5177 
                     /label="Restriction Enzyme"  
     misc_feature    5962..6634 
                     /label="ColE1"  
     misc_feature    7064..7858 
                     /label="Kan/Neo"  
     misc_feature    7893..8195 
                     /label="SV40 ori"  
     misc_feature    8281..8758 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4962..4968 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4962 
                     /label="MYO3B"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAACATCTG TATGGATTAT TTCACTATAA TCCTATGATG CTTGGACTTG 
     1081 AATCACTTCC AGATCCCACA GACACCTGGG AAATTATAGA GACCATTGGT AAAGGCACCT 
     1141 ATGGCAAAGT CTACAAGGTA ACTAACAAGA GAGATGGGAG CCTGGCTGCA GTGAAAATTC 
     1201 TGGATCCAGT CAGTGATATG GATGAAGAAA TTGAGGCAGA ATACAACATT TTGCAGTTCC 
     1261 TTCCTAATCA TCCCAATGTT GTAAAGTTTT ATGGGATGTT TTACAAAGCG GATCACTGTG 
     1321 TAGGGGGACA GCTGTGGCTG GTCCTGGAGC TGTGTAATGG GGGCTCAGTC ACTGAGCTTG 
     1381 TCAAAGGTCT ACTCAGATGT GGCCAGCGGT TGGATGAAGC AATGATCTCA TACATCTTGT 
     1441 ACGGGGCCCT CTTGGGCCTT CAGCATTTGC ACAACAACCG AATCATCCAC CGTGATGTGA 
     1501 AGGGGAATAA CATTCTTCTG ACAACAGAAG GAGGAGTTAA GCTCGTTGAC TTTGGTGTTT 
     1561 CAGCTCAACT CACCAGTACA CGTCTGCGGA GAAACACATC TGTTGGCACC CCGTTCTGGA 
     1621 TGGCCCCTGA GGTCATTGCC TGTGAGCAGC AGTATGACTC TTCCTATGAC GCTCGCTGTG 
     1681 ACGTCTGGTC CTTGGGGATC ACAGCTATTG AACTGGGGGA TGGAGACCCT CCCCTCTTTG 
     1741 ACATGCATCC TGTGAAAACA CTCTTTAAGA TTCCAAGAAA TCCTCCACCT ACTTTACTTC 
     1801 ATCCAGAAAA ATGGTGTGAA GAATTCAACC ACTTTATTTC ACAGTGTCTT ATTAAGGATT 
     1861 TTGAAAGGCG ACCTTCCGTC ACACATCTCC TTGACCACCC ATTTATTAAA GGAGTACATG 
     1921 GAAAAGTTCT GTTTCTGCAA AAACAGCTGG CCAAGGTTCT CCAAGACCAG AAGCATCAAA 
     1981 ATCCTGTTGC TAAAACCAGG CATGAGAGGA TGCATACCAG AAGACCTTAT CATGTGGAAG 
     2041 ATGCTGAAAA ATACTGCCTT GAGGATGATT TGGTCAACCT AGAGGTTCTG GATGAGGATA 
     2101 CAATTATCCA TCAGTTGCAG AAACGTTATG CAGACTTGCT AATTTACACA TATGTTGGAG 
     2161 ACATCTTAAT TGCCTTAAAC CCCTTCCAGA ATCTAAGCAT ATACTCTCCA CAGTTTTCCA 
     2221 GACTTTATCA TGGGGTGAAA CGCGCCTCCA ATCCCCCCCA CATATTTGCA TCAGCAGATG 
     2281 CTGCTTACCA GTGCATGGTT ACTCTCAGCA AAGACCAGTG CATTGTCATC AGCGGAGAGA 
     2341 GTGGCTCTGG GAAGACAGAA AGCGCCCACC TGATTGTTCA GCATTTGACT TTCTTGGGAA 
     2401 AGGCCAATAA TCAGACCTTG AGAGAGAAAA TTCTACAAGT CAACTCCCTG GTGGAAGCCT 
     2461 TTGGGAACTC ATGCACTGCC ATCAATGACA ACTCGAGCCG TTTTGGAAAA TATCTGGAAA 
     2521 TGATGTTTAC ACCAACTGGA GTTGTGATGG GGGCAAGAAT CTCTGAATAT CTCCTGGAAA 
     2581 AATCCAGAGT TATAAAACAG GCAGCGAGAG AGAAAAATTT TCATATATTT TACTATATTT 
     2641 ATGCTGGTCT TCATCACCAA AAGAAGCTTT CTGATTTCAG ACTTCCTGAG GAAAAACCTC 
     2701 CTAGGTACAT AGCTGATGAA ACTGGAAGGG TGATGCACGA CATAACTTCC AAGGAGTCTT 
     2761 ACAGAAGACA ATTCGAAGCA ATTCAGCATT GCTTCAGGAT TATAGGGTTC ACGGACAAAG 
     2821 AGGTGCACTC AGTGTACAGA ATTTTGGCTG GGATTTTGAA TATTGGGAAC ATTGAGTTCG 
     2881 CAGCTATTTC CTCTCAACAT CAGACTGATA AAAGTGAGGT GCCCAATGCT GAAGCTTTGC 
     2941 AAAATGCTGC CTCTGTTCTG TGCATTAGCC CTGAAGAGCT CCAGGAGGCC CTCACCTCCC 
     3001 ACTGTGTGGT CACCCGGGGC GAGACCATCA TCCGTGCCAA CACTGTAGAC AGGGCTGCGG 
     3061 ACGTTCGAGA CGCCATGTCC AAAGCCCTGT ATGGGAGGCT CTTCAGCTGG ATTGTGAATC 
     3121 GCATTAATAC ACTCCTGCAG CCAGACGAAA ACATATGTAG TGCAGGAGGT GGAATGAATG 
     3181 TGGGGATCTT GGATATCTTT GGATTCGAGA ATTTTCAGAG AAATTCATTT GAGCAGCTCT 
     3241 GCATAAACAT CGCCAATGAG CAAATCCAGT ACTATTTCAA TCAGCATGTT TTTGCTCTTG 
     3301 AGCAGATGGA ATATCAGAAT GAAGGCATTG ATGCTGTACC CGTGGAATAT GAGGACAACC 
     3361 GCCCGCTCTT GGACATGTTC CTCCAGAAAC CCCTGGGACT GCTTGCACTT TTGGATGAGG 
     3421 AAAGTCGGTT TCCCCAAGCA ACTGACCAGA CCCTGGTTGA TAAATTTGAA GATAATCTAC 
     3481 GATGCAAATA CTTCTGGAGG CCCAAAGGAG TGGAACTGTG CTTTGGCATT CAGCATTATG 
     3541 CTGGAAAGGT ATTATATGAT GCTTCTGGGG TTCTTGAGAA AAATAGAGAC ACTCTCCCTG 
     3601 CCGATGTGGT TGTGGTCCTG AGAACGTCAG AAAACAAGCT TCTTCAGCAG CTCTTCTCAA 
     3661 TCCCTCTGAC CAAAACAGGT AATTTGGCCC AGACAAGAGC TAGGATAACA GTGGCCTCAA 
     3721 GTTCTTTGCC TCCACATTTC AGTGCTGGGA AAGCCAAGGT GGACACTCTG GAGGTGATAC 
     3781 GGCATCCGGA AGAAACCACC AACATGAAGA GGCAAACTGT GGCTTCTTAC TTCCGGTATT 
     3841 CTCTGATGGA CCTGCTCTCC AAAATGGTGG TTGGACAGCC CCACTTTGTG CGCTGCATTA 
     3901 AACCCAATGA TGACCGAGAG GCCCTGCAGT TCTCTCGAGA GAGGGTGCTG GCCCAGCTCC 
     3961 GCTCCACAGG GATTCTGGAG ACAGTCAGCA TCCGCCGCCA GGGCTATTCC CACCGCATCC 
     4021 TTTTTGAAGA ATTTGTGAAA AGGTATTATT ACTTGGCATT CACAGCACAT CAAACACCTC 
     4081 TTGCTAGCAA AGAGAGCTGT GTGGCTATCT TGGAAAAGTC CAGATTAGAT CACTGGGTAC 
     4141 TGGGAAAAAC AAAGGTTTTT CTCAAATATT ACCATGTTGA GCAATTAAAT TTGCTGCTTC 
     4201 GAGAAGTCAT AGGCAGAGTG GTTGTGCTGC AGGCATATAC CAAGGGGTGG CTTGGAGCCA 
     4261 GGAGATACAA AAGGGTCAGA GAGAAGAGAG AGAAGGGAGC CATTGCCATC CAGTCAGGGG 
     4321 ACACTTCAAA CCAAAGCAGT GGGCCACATT CCCCCGTCGC AGCAGGTACG AGGGGAAGTG 
     4381 CCGAGGTTCA AGACTGCAGC GAGCCTGGTG ACCATAAAGT TCTCAGGGGC TCTGTACATC 
     4441 GTAGGAGCCA TTCACAAGCA GAATCCAACA ATGGCCGTAC ACAGACTTCA AGCAACTCTC 
     4501 CTGCTGTCAC AGAGAAAAAT GGGCATTCAC AAGCCCAGAG TTCTCCAAAA GGGTGCGATA 
     4561 TCTTCGCAGG ACATGCAAAC AAGCACTCGG TTTCTGGGAC TGATTTGCTG TCTTCTCGGA 
     4621 TATGCCATCC TGCTCCAGAT CAGCAAGGAT TGAGTCTCTG GGGAGCCCCT CAAAAGCCTG 
     4681 GTTCAGAAAA TGGTCTTGCA CAGAAGCATC GAACACCTCG CCGACGATGT CAGCAGCCCA 
     4741 AAATGCTGAG TAGCCCTGAG GACACCATGT ACTATAACCA GTTAAATGGA ACTCTAGAAT 
     4801 ATCAAGGGAG CAAGAGGAAG CCAAGAAAAC TTGGCCAAAT CAAAGTACTT GATGGGGAAG 
     4861 ATGAATATTA CAAATCTCTG TCACCAGTGG ACTGTATCCC TGAGGAGAAC AACTCAGCCC 
     4921 ACCCTTCCTT TTTTTCTTCA TCCTCAAAAG GAGACTCTTT TGCTCAACAT ACGCGTACGC 
     4981 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     5041 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     5101 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     5161 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     5221 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     5281 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     5341 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     5401 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     5461 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     5521 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     5581 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     5641 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     5701 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     5761 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     5821 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     5881 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     5941 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     6001 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     6061 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     6121 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     6181 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     6241 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     6301 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     6361 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     6421 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     6481 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     6541 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     6601 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     6661 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     6721 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     6781 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     6841 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     6901 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     6961 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     7021 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     7081 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     7141 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     7201 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     7261 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     7321 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     7381 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     7441 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     7501 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     7561 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     7621 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     7681 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     7741 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     7801 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     7861 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     7921 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     7981 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     8041 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     8101 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     8161 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     8221 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     8281 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     8341 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     8401 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     8461 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     8521 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     8581 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     8641 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     8701 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     8761 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     8821 TTT
//