LOCUS       RC216074                8469 bp    DNA     circular  SYN 
DEFINITION  FMN1 (Myc-DDK-tagged)-Human formin 1 (FMN1)
ACCESSION   NM_001103184
VERSION     NM_001103184.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4626..4717 
                     /label="Myc-DDK"  
     misc_feature    4747..5332 
                     /label="PolyA signal"  
     misc_feature    4804..4823 
                     /label="Restriction Enzyme"  
     misc_feature    5608..6280 
                     /label="ColE1"  
     misc_feature    6710..7504 
                     /label="Kan/Neo"  
     misc_feature    7539..7841 
                     /label="SV40 ori"  
     misc_feature    7927..8404 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4608..4614 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4608 
                     /label="FMN1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGAACGTT GATAATTCTC TGGATGGCAG TGATGTGTCA GAACCAGCAA 
     1081 AACCAGAAGC CGGACTTGAA GTGGCTCAGT CGATCCTGAG TAAGTTCTCT ATGAAATCCC 
     1141 TATTTGGGTT TACGAGTAAA TTAGAATCTG TGAATCCAGA AGAGGAAGAT GCTGTGCTGA 
     1201 AAGCATTCCA TAGTTTAGAC GTGAATCCCA CATCTCAGCA GGATGATTCC AGCAATGGCT 
     1261 TGGATCCACA GGAAGCAGGG TCTCGGGTTT CACCTGACCT CGGAAATGAT GAGAAAATTG 
     1321 CAAGCGTGGA GACAGAATCA GAGGGAAGTC AGAGAAAAGA AGCAGGGACA TCTCTCCTTG 
     1381 CTCAAGAGCT GCTTCCACTG TCTACTTTGA AAGGGACAAA AGACGATGTC ATTTGTGTTC 
     1441 GTGGGACCCT GGTGCACACC ACCAGTGATT CTGACTCTGA TGATGGTGGC CAGGAGCCAG 
     1501 AGGAGGGAAG CAGCACCAAT GGCCCAAAGT CCCCTAGTGG TGTTTTATCT GAGCCATCTC 
     1561 AGGAGTCCAA AGAAAACCCA GGAGGTTTCA GGGAAAATAC TGTCACTGGG GAAATGAATG 
     1621 GTGCAGAGCT CTGTGCAGAA GATCCTCAAA GGATTCCACC TGAGATGAGC AGCAAACTGG 
     1681 AAGCTGGCAA CGGTGGTCTT CAGACAGAGC GTCGCCCCAG CCAGGACCAG GTGGGAGAAG 
     1741 AAGGGTCCCA AGACCTACCT GCAGTAACAA ACCAGAATTC GAGTGTTGGT ATCACTGAGA 
     1801 GTGCCTCTTC TAAAAAAGAA GTCTCTGGAG AGAAGTCATT CCAACTTCCA GCTTTTTTCA 
     1861 GTGGGCTTCG TGTGCTGAAG AAGGGGGCTA CCGCTGAGGG AGGAGAGACC ATCACGGAAA 
     1921 TTAAACCAAA GGATGGGGAC TTAGCTTTGC TCAAATTGAC GCAGCCTGTG CAGAAGTCCT 
     1981 TAGTGCAGGC AGGGCTGCAG ACAGTGAAGA GTGAGAAGAA AGCAACTGAT CCGAAGGCCA 
     2041 CTCCCACTCT CCTGGAGCAG CTCTCTCTGC TGCTCAACAT TGACATGCCC AAAACTGAAC 
     2101 CGAAGGGAGC AGACCCTGAG TCGCCCAGGA GAGAAGAGAT GGGCTGCAAT GCTGACCAAG 
     2161 AGAGCCAGAG CGGCCCTGGA GTACCACAAA CCCAGGGTGG TGAGGTCAAG CCAAAGTCGC 
     2221 CAGAGACAGC CCTGGAGGCT TTTAAAGCCC TATTCATCCG GCCCCCCAGA AAGGGGACCA 
     2281 CAGCTGACAC CTCTGAGCTG GAAGCTCTCA AGCGCAAGAT GAGACATGAG AAGGAGTCGC 
     2341 TAAGAGCTGT GTTTGAACGG TCCAATTCTA AGCCAGCGGA TGGCCCCTCT GATTCCAAAA 
     2401 GTCCTGACCA CAGCCTGACT GAGCAGGATG ACAGGACTCC TGGCAGACTT CAAGCTGTCT 
     2461 GGCCACCCCC AAAGACAAAA GACACAGAAG AAAAAGTGGG ACTGAAGTAC ACTGAAGCAG 
     2521 AATACCAAGC TGCTATTTTA CACTTGAAGA GGGAGCACAA AGAAGAAATT GAAAACCTGC 
     2581 AGGCACAGTT TGAACTTCGG GCATTTCATA TCCGGGGCGA GCATGCAATG ATAACAGCGA 
     2641 GACTAGAAGA AACCATTGAA AATCTGAAAC ACGAGCTAGA ACACAGATGG CGAGGGGGTT 
     2701 GTGAAGAGAG GAAAGATGTG TGCATTTCCA CCGATGATGA CTGCCCTCCA AAGACCTTCA 
     2761 GAAATGTGTG CGTCCAGACA GACAGAGAGA CCTTCCTCAA GCCCTGTGAA AGTGAAAGCA 
     2821 AGACAACCAG AAGTAATCAA TTAGTACCCA AAAAGCTGAA TATCTCCTCT TTAAGCCAGC 
     2881 TCTCACCCCC AAATGACCAC AAAGACATCC ATGCAGCACT CCAGCCAATG GAGGGCATGG 
     2941 CATCAAATCA GCAGAAGGCA TTGCCTCCGC CTCCCGCATC CATCCCTCCC CCTCCGCCCC 
     3001 TCCCCTCAGG ACTTGGATCT TTGTCTCCCG CACCTCCAAT GCCACCTGTG AGTGCTGGGC 
     3061 CACCGCTACC ACCTCCGCCT CCTCCACCGC CACCTCTACC TCCACCTTCA AGTGCTGGAC 
     3121 CTCCACCACC CCCACCCCCA CCCCCACTTC CTAACTCCCC TGCCCCACCC AACCCTGGAG 
     3181 GGCCTCCTCC TGCTCCACCA CCCCCAGGAC TTGCACCCCC ACCTCCCCCT GGACTCTTCT 
     3241 TTGGACTTGG CTCTTCTTCC AGTCAATGTC CTCGAAAACC AGCCATCGAG CCCAGTTGTC 
     3301 CCATGAAGCC TTTATATTGG ACTAGGATAC AAATAAGTGA TAGGAGCCAA AATGCTACAC 
     3361 CAACCTTATG GGACTCCTTA GAAGAACCTG ACATTCGGGA CCCCAGTGAA TTTGAGTATT 
     3421 TATTCTCCAA AGACACAACT CAACAGAAGA AAAAACCTCT GTCAGAGACT TATGAGAAGA 
     3481 AAAACAAGGT CAAAAAGATC ATCAAATTGT TGGATGGAAA ACGATCTCAA ACTGTGGGAA 
     3541 TCTTGATATC TAGTTTACAT TTAGAAATGA AGGATATCCA ACAGGCCATT TTCAATGTGG 
     3601 ATGACTCCGT GGTTGATCTG GAGACCCTGG CAGCCTTATA TGAAAACAGA GCCCAAGAGG 
     3661 ATGAGCTGGT TAAAATAAGA AAGTATTACG AGACATCCAA AGAAGAAGAA CTGAAGCTGC 
     3721 TGGATAAACC TGAGCAATTT TTACATGAGT TAGCCCAGAT TCCTAATTTT GCTGAACGTG 
     3781 CCCAGTGCAT AATCTTCAGA TCTGTCTTTT CTGAGGGTAT CACCTCCTTG CACAGAAAGG 
     3841 TAGAGATCAT CACGCGAGCT TCTAAGGACT TGCTGCACGT GAAGAGCGTG AAGGATATTT 
     3901 TAGCTCTCAT CTTGGCTTTT GGAAATTATA TGAATGGAGG AAATAGGACT CGGGGACAAG 
     3961 CCGATGGATA TAGCTTAGAA ATTCTGCCCA AACTCAAGGA TGTCAAAAGT CGGGATAATG 
     4021 GGATTAATCT GGTGGACTAC GTTGTTAAGT ATTACCTGCG TTACTATGAT CAGGAAGCTG 
     4081 GAACAGAAAA GAGTGTTTTC CCCTTGCCGG AACCACAGGA TTTCTTTCTG GCCTCCCAAG 
     4141 TCAAGTTTGA AGACCTCATA AAAGATTTGA GAAAACTGAA GAGGCAACTA GAAGCAAGTG 
     4201 AGAAACAGAT GGTGGTGGTG TGCAAGGAGT CCCCAAAGGA GTATCTCCAG CCTTTCAAGG 
     4261 ACAAACTAGA GGAGTTCTTC CAAAAAGCCA AAAAAGAGCA TAAGATGGAA GAAAGTCACT 
     4321 TGGAGAATGC ACAGAAAAGT TTTGAAACAA CAGTACGATA TTTTGGGATG AAGCCAAAGT 
     4381 CTGGTGAGAA GGAGATCACA CCCAGCTACG TGTTTATGGT GTGGTATGAG TTCTGCAGTG 
     4441 ACTTCAAGAC AATTTGGAAA CGGGAGAGTA AAAACATATC TAAAGAAAGA TTGAAAATGG 
     4501 CTCAGGAATC AGTCAGCAAG TTGACTTCAG AGAAGAAAGT GGAGACAAAG AAAATCAATC 
     4561 CCACTGCTAG CCTGAAAGAA AGACTGCGTC AGAAGGAAGC CAGTGTGACC ACTAACACGC 
     4621 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     4681 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     4741 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     4801 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     4861 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     4921 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     4981 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     5041 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     5101 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     5161 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     5221 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     5281 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     5341 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     5401 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     5461 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     5521 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     5581 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     5641 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     5701 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     5761 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     5821 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     5881 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     5941 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     6001 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     6061 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     6121 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     6181 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     6241 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     6301 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     6361 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     6421 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     6481 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     6541 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     6601 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     6661 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     6721 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     6781 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     6841 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     6901 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     6961 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     7021 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     7081 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     7141 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     7201 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     7261 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     7321 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     7381 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     7441 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     7501 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     7561 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     7621 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     7681 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     7741 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     7801 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7861 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     7921 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     7981 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     8041 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     8101 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     8161 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     8221 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     8281 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     8341 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     8401 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     8461 GCGAATTTT
//