LOCUS       RC216038                7200 bp    DNA     circular  SYN 
DEFINITION  SEMA3E (Myc-DDK-tagged)-Human sema domain, immunoglobulin domain (Ig), short basic domain, secreted, (semaphorin) 3E (SEMA3E), transcript variant 1
ACCESSION   NM_012431
VERSION     NM_012431.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3355..3446 
                     /label="Myc-DDK"  
     misc_feature    3476..4061 
                     /label="PolyA signal"  
     misc_feature    3533..3552 
                     /label="Restriction Enzyme"  
     misc_feature    4337..5009 
                     /label="ColE1"  
     misc_feature    5439..6233 
                     /label="Kan/Neo"  
     misc_feature    6268..6570 
                     /label="SV40 ori"  
     misc_feature    6656..7133 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3345..3353 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3345 
                     /label="SEMA3E"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCATCCGCG GGGCACATTA TCACCTTGCT CCTGTGGGGT TACTTACTGG 
     1081 AGCTTTGGAC AGGAGGTCAT ACAGCTGATA CTACCCACCC CCGGTTACGC CTGTCACATA 
     1141 AAGAGCTCTT GAATCTGAAC AGAACATCAA TATTTCATAG CCCTTTTGGA TTTCTTGATC 
     1201 TCCATACAAT GCTGCTGGAT GAATATCAAG AGAGGCTCTT CGTGGGAGGC AGGGACCTTG 
     1261 TATATTCCCT CAGCTTGGAG AGAATCAGTG ACGGCTATAA AGAGATACAC TGGCCGAGTA 
     1321 CAGCTCTAAA AATGGAAGAA TGCATAATGA AGGGAAAAGA TGCGGGTGAA TGTGCAAATT 
     1381 ATGTTCGGGT TTTGCATCAC TATAACAGGA CACACCTTCT GACCTGTGGT ACTGGAGCTT 
     1441 TTGATCCAGT TTGTGCCTTC ATCAGAGTTG GATATCATTT GGAGGATCCT CTGTTTCACC 
     1501 TGGAATCACC CAGATCTGAG AGAGGAAGGG GCAGATGTCC TTTTGACCCC AGCTCCTCCT 
     1561 TCATCTCCAC TTTAATTGGT AGTGAATTGT TTGCTGGACT CTACAGTGAC TACTGGAGCA 
     1621 GAGACGCTGC GATCTTCCGC AGCATGGGGC GACTGGCCCA TATCCGCACT GAGCATGACG 
     1681 ATGAGCGTCT GTTGAAAGAA CCAAAATTTG TAGGTTCATA CATGATTCCT GACAATGAAG 
     1741 ACAGAGATGA CAACAAAGTA TATTTCTTTT TTACTGAGAA GGCACTGGAG GCAGAAAACA 
     1801 ATGCTCACGC AATTTACACC AGGGTCGGGC GACTCTGTGT GAATGATGTA GGAGGGCAGA 
     1861 GAATACTGGT GAATAAGTGG AGCACTTTCC TAAAAGCGAG ACTCGTTTGC TCAGTACCAG 
     1921 GAATGAATGG AATTGACACA TATTTTGATG AATTAGAGGA CGTTTTTTTG CTACCTACCA 
     1981 GAGATCATAA GAATCCAGTG ATATTTGGAC TCTTTAACAC TACCAGTAAT ATTTTTCGAG 
     2041 GGCATGCTAT ATGTGTCTAT CACATGTCTA GCATTCGGGC AGCCTTCAAC GGACCATATG 
     2101 CACATAAGGA AGGACCTGAA TACCACTGGT CAGTCTATGA AGGAAAAGTC CCTTATCCAA 
     2161 GGCCTGGTTC TTGTGCCAGC AAAGTAAATG GAGGGAGATA CGGAACCACC AAGGACTATC 
     2221 CTGATGATGC CATCCGATTT GCAAGAAGTC ATCCACTAAT GTACCAGGCC ATAAAACCTG 
     2281 CCCATAAAAA ACCAATATTG GTAAAAACAG ATGGAAAATA TAACCTGAAA CAAATAGCAG 
     2341 TAGATCGAGT GGAAGCTGAG GATGGCCAAT ATGACGTCTT GTTTATTGGG ACAGATAATG 
     2401 GAATTGTGCT GAAAGTAATC ACAATTTACA ACCAAGAAAT GGAATCAATG GAAGAAGTAA 
     2461 TTCTAGAAGA ACTTCAGATA TTCAAGGATC CAGTTCCTAT TATTTCTATG GAGATTTCTT 
     2521 CAAAGCGGCA ACAGCTGTAT ATTGGATCTG CTTCTGCTGT GGCTCAAGTC AGATTCCATC 
     2581 ACTGTGACAT GTATGGAAGT GCTTGTGCTG ACTGCTGCCT GGCTCGAGAC CCTTACTGTG 
     2641 CCTGGGATGG CATATCCTGC TCCCGGTATT ACCCAACAGG CACACATGCA AAAAGGCGTT 
     2701 TCCGGAGACA AGATGTTCGA CATGGAAATG CAGCTCAGCA GTGCTTTGGA CAACAGTTTG 
     2761 TTGGGGATGC TTTGGATAAG ACTGAAGAAC ATCTGGCTTA TGGCATAGAG AACAACAGTA 
     2821 CTTTGCTGGA ATGTACCCCA CGATCTTTAC AAGCGAAAGT TATCTGGTTT GTACAGAAAG 
     2881 GACGTGAGAC AAGAAAAGAG GAGGTGAAGA CAGATGACAG AGTGGTTAAG ATGGACCTTG 
     2941 GTTTACTCTT CCTAAGGTTA CACAAATCAG ATGCTGGGAC CTATTTTTGC CAGACAGTAG 
     3001 AGCATAGCTT TGTCCATACG GTCCGTAAAA TCACCTTGGA GGTAGTGGAA GAGGAGAAAG 
     3061 TCGAGGATAT GTTTAACAAG GACGATGAGG AGGACAGGCA TCACAGGATG CCTTGTCCTG 
     3121 CTCAGAGTAG CATCTCGCAG GGAGCAAAAC CATGGTACAA GGAATTCTTG CAGCTGATCG 
     3181 GTTATAGCAA CTTCCAGAGA GTGGAAGAAT ACTGCGAGAA AGTATGGTGC ACAGATAGAA 
     3241 AGAGGAAAAA GCTTAAAATG TCACCCTCCA AGTGGAAGTA TGCCAACCCT CAGGAAAAGA 
     3301 AGCTCCGTTC CAAACCTGAG CATTACCGCC TGCCCAGGCA CACGCTGGAC TCCACGCGGC 
     3361 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     3421 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     3481 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     3541 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     3601 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     3661 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     3721 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     3781 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     3841 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     3901 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     3961 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     4021 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     4081 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     4141 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     4201 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     4261 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     4321 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     4381 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     4441 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     4501 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     4561 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     4621 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     4681 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     4741 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     4801 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     4861 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     4921 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     4981 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     5041 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     5101 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     5161 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     5221 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     5281 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     5341 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     5401 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     5461 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     5521 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     5581 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     5641 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     5701 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     5761 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     5821 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     5881 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     5941 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     6001 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     6061 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     6121 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     6181 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     6241 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     6301 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     6361 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     6421 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     6481 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     6541 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     6601 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     6661 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     6721 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     6781 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     6841 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     6901 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     6961 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     7021 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     7081 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     7141 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//