LOCUS       RC215981                9045 bp    DNA     circular  SYN 
DEFINITION  COL15A1 (Myc-DDK-tagged)-Human collagen, type XV, alpha 1 (COL15A1)
ACCESSION   NM_001855
VERSION     NM_001855.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5202..5293 
                     /label="Myc-DDK"  
     misc_feature    5323..5908 
                     /label="PolyA signal"  
     misc_feature    5380..5399 
                     /label="Restriction Enzyme"  
     misc_feature    6184..6856 
                     /label="ColE1"  
     misc_feature    7286..8080 
                     /label="Kan/Neo"  
     misc_feature    8115..8417 
                     /label="SV40 ori"  
     misc_feature    8503..8980 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5184..5190 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5184 
                     /label="COL15A1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCACCAAGG AGGAACAACG GGCAGTGCTG GTGTCTGCTG ATGCTGCTCT 
     1081 CGGTCTCCAC GCCCCTCCCT GCTGTCACCC AGACCCGCGG TGCGACAGAG ACTGCTTCCC 
     1141 AGGGTCACCT GGACCTCACG CAGCTCATCG GTGTCCCGCT GCCCTCGTCC GTATCCTTTG 
     1201 TCACAGGCTA TGGTGGCTTC CCGGCCTACA GTTTCGGGCC TGGTGCCAAT GTTGGCCGCC 
     1261 CAGCCAGGAC TCTCATCCCA TCCACCTTCT TCAGGGACTT CGCCATCAGC GTCGTGGTGA 
     1321 AGCCCAGCAG CACCCGTGGT GGCGTGCTCT TCGCCATCAC TGACGCCTTC CAGAAGGTCA 
     1381 TCTACCTGGG CCTGCGGCTC TCAGGTGTGG AGGACGGCCA CCAGCGGATC ATCCTCTACT 
     1441 ACACGGAGCC AGGCTCCCAT GTGTCCCAAG AGGCTGCTGC CTTCTCGGTG CCTGTGATGA 
     1501 CCCACAGGTG GAACCGCTTC GCCATGATTG TCCAGGGTGA GGAAGTGACC CTCCTCGTGA 
     1561 ACTGTGAGGA GCACAGCCGC ATCCCCTTCC AGCGGTCCTC CCAGGCTTTG GCTTTTGAGT 
     1621 CCAGCGCTGG AATCTTCATG GGCAATGCAG GAGCTACAGG GCTCGAGAGA TTCACTGGCT 
     1681 CCCTCCAGCA GCTCACCGTG CACCCCGACC CCAGGACTCC CGAGGAGCTG TGTGACCCTG 
     1741 AAGAGTCCTC GGCATCTGGA GAGACCAGTG GGCTGCAGGA GGCAGACGGA GTAGCTGAGA 
     1801 TCTTAGAAGC CGTCACCTAC ACTCAAGCCT CGCCCAAAGA AGCAAAAGTT GAACCCATAA 
     1861 ACACACCTCC AACTCCATCC TCCCCCTTTG AAGACATGGA ACTTTCTGGT GAACCTGTAC 
     1921 CCGAGGGGAC CCTGGAAACC ACCAACATGA GCATCATCCA GCACAGCAGC CCCAAACAAG 
     1981 GGTCTGGTGA GATCCTGAAT GACACACTGG AGGGGGTTCA TTCTGTGGAT GGTGACCCCA 
     2041 TTACTGACAG CGGCTCAGGG GCTGGGGCCT TCCTTGACAT TGCTGAAGAA AAGAATTTAG 
     2101 CAGCAACAGC AGCGGGGCTG GCCGAGGTGC CCATCAGCAC TGCTGGAGAA GCAGAGGCCA 
     2161 GCAGTGTGCC CACCGGGGGA CCAACCCTCT CTATGTCCAC GGAGAACCCA GAGGAAGGGG 
     2221 TCACTCCAGG TCCAGATAAT GAAGAGCGTT TAGCAGCAAC AGCAGCAGGG GAGGCCGAGG 
     2281 CACTCGCCAG CATGCCTGGG GAAGTGGAGG CCAGTGGTGT GGCCCCCGGG GAGCTGGACC 
     2341 TCTCCATGTC CGCCCAGAGC CTCGGGGAAG AGGCCACTGT GGGTCCAAGC AGTGAAGACA 
     2401 GTTTAACAAC AGCTGCAGCT GCAACCGAAG TGTCCCTCAG TACTTTTGAG GATGAGGAAG 
     2461 CCAGTGGGGT CCCCACAGAT GGCCTGGCTC CCCTCACAGC CACCATGGCC CCTGAGCGGG 
     2521 CAGTCACTTC TGGTCCTGGT GATGAAGAAG ACTTGGCAGC AGCCACAACA GAGGAGCCCC 
     2581 TCATCACAGC TGGGGGTGAA GAGTCCGGCA GCCCTCCCCC TGATGGGCCA CCGCTGCCCC 
     2641 TGCCCACAGT GGCTCCTGAA AGATGGATCA CTCCAGCTCA AAGAGAACAT GTGGGAATGA 
     2701 AAGGACAGGC TGGGCCCAAA GGAGAAAAGG GTGATGCTGG GGAGGAGCTT CCTGGCCCTC 
     2761 CTGAACCTTC TGGGCCTGTT GGACCCACGG CAGGAGCAGA AGCAGAGGGC TCTGGCCTAG 
     2821 GCTGGGGCTC GGACGTCGGC TCTGGCTCTG GTGACCTGGT GGGCAGTGAG CAGCTGCTGA 
     2881 GAGGTCCTCC AGGACCCCCA GGGCCACCTG GCTTACCTGG GATTCCAGGA AAACCAGGAA 
     2941 CTGATGTTTT CATGGGACCC CCTGGATCTC CTGGAGAGGA TGGACCTGCT GGTGAACCTG 
     3001 GGCCCCCGGG CCCTGAGGGA CAGCCTGGAG TTGATGGAGC CACCGGCCTT CCCGGGATGA 
     3061 AAGGGGAGAA GGGAGCAAGA GGGCCTAATG GCTCAGTTGG TGAAAAGGGT GACCCTGGCA 
     3121 ACAGAGGCTT ACCTGGACCC CCGGGGAAAA AGGGACAAGC TGGCCCTCCT GGGGTCATGG 
     3181 GACCCCCAGG GCCTCCTGGA CCCCCTGGGC CCCCAGGCCC TGGATGCACA ATGGGACTTG 
     3241 GATTCGAGGA TACCGAAGGC TCTGGAAGCA CCCAGCTATT GAATGAACCC AAACTCTCCA 
     3301 GACCAACGGC TGCAATTGGT CTCAAAGGAG AGAAAGGAGA CCGGGGACCC AAGGGAGAAA 
     3361 GGGGGATGGA TGGAGCCAGT ATTGTGGGAC CCCCTGGGCC GAGAGGGCCA CCTGGGCACA 
     3421 TCAAGGTCTT GTCTAATTCC TTGATCAATA TCACCCATGG ATTCATGAAT TTCTCGGACA 
     3481 TTCCTGAGCT GGTGGGGCCT CCGGGGCCGG ACGGGTTGCC TGGGCTGCCA GGATTTCCAG 
     3541 GTCCTAGAGG ACCAAAAGGT GACACTGGTT TACCTGGCTT TCCAGGACTA AAAGGAGAAC 
     3601 AGGGCGAGAA GGGAGAGCCG GGTGCCATCC TGACAGAGGA CATTCCTCTG GAAAGGCTGA 
     3661 TGGGGAAAAA GGGTGAACCT GGAATGCATG GAGCCCCAGG ACCAATGGGG CCCAAAGGAC 
     3721 CACCAGGACA TAAAGGAGAA TTTGGCCTTC CCGGGCGACC TGGTCGCCCA GGACTGAATG 
     3781 GCCTCAAGGG TACCAAAGGA GATCCAGGGG TCATTATGCA GGGCCCACCT GGCTTACCTG 
     3841 GCCCTCCAGG CCCCCCTGGG CCACCTGGAG CTGTGATTAA CATCAAAGGA GCCATTTTCC 
     3901 CAATACCCGT CCGACCACAC TGCAAAATGC CAGTTGATAC TGCTCATCCT GGGAGTCCAG 
     3961 AGCTCATCAC TTTTCACGGT GTTAAAGGAG AGAAAGGATC CTGGGGTCTT CCTGGCTCAA 
     4021 AGGGAGAAAA AGGCGACCAG GGAGCCCAGG GACCACCAGG TCCTCCACTT GATCTAGCTT 
     4081 ACCTGAGACA CTTTCTGAAC AACTTGAAGG GGGAGAATGG AGACAAGGGG TTCAAAGGTG 
     4141 AAAAAGGAGA AAAAGGAGAC ATTAATGGCA GCTTCCTTAT GTCTGGGCCT CCAGGCCTGC 
     4201 CCGGAAATCC AGGCCCGGCT GGCCAAAAAG GGGAGACAGT CGTTGGGCCC CAAGGACCCC 
     4261 CAGGTGCTCC TGGTCTGCCT GGGCCACCTG GCTTTGGAAG ACCTGGTGAT CCTGGGCCAC 
     4321 CGGGGCCCCC GGGGCCACCA GGACCTCCAG CTATCCTGGG AGCAGCTGTG GCCCTTCCAG 
     4381 GTCCCCCTGG CCCTCCAGGA CAGCCAGGGC TTCCCGGATC CAGAAACCTG GTCACAGCAT 
     4441 TCAGCAACAT GGATGACATG CTGCAGAAAG CGCATTTGGT TATAGAAGGA ACATTCATCT 
     4501 ACCTGAGGGA CAGCACTGAG TTTTTCATTC GTGTTAGAGA TGGCTGGAAA AAATTACAGC 
     4561 TGGGAGAACT GATCCCCATT CCTGCCGACA GCCCTCCACC CCCTGCGCTT TCCAGCAACC 
     4621 CACATCAGCT TCTGCCTCCA CCAAACCCTA TTTCAAGTGC CAATTATGAG AAGCCTGCTC 
     4681 TGCATTTGGC TGCTCTGAAC ATGCCATTTT CTGGGGACAT TCGAGCTGAT TTTCAGTGCT 
     4741 TCAAGCAGGC CAGAGCTGCA GGACTGTTGT CCACCTACCG AGCATTCTTA TCTTCCCATT 
     4801 TGCAAGATCT GTCCACCATT GTGAGGAAAG CAGAGAGATA CAGCCTTCCC ATAGTGAACC 
     4861 TCAAGGGCCA AGTACTTTTT AATAATTGGG ACTCAATTTT TTCTGGCCAC GGAGGTCAGT 
     4921 TCAATATGCA TATTCCAATA TACTCCTTTG ATGGTCGAGA CATAATGACA GATCCTTCTT 
     4981 GGCCCCAGAA AGTCATTTGG CATGGCTCCA GCCCCCATGG CGTCCGCCTT GTGGATAACT 
     5041 ACTGTGAAGC ATGGCGAACC GCGGACACAG CGGTCACGGG ACTTGCCTCC CCGCTGAGCA 
     5101 CGGGGAAGAT TCTGGACCAG AAAGCATACA GCTGTGCTAA TCGGCTAATT GTCCTATGTA 
     5161 TCGAAAACAG TTTCATGACA GACGCTAGGA AGACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     5221 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     5281 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     5341 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     5401 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     5461 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     5521 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     5581 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     5641 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     5701 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     5761 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5821 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5881 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5941 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     6001 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     6061 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     6121 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     6181 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     6241 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     6301 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     6361 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     6421 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     6481 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     6541 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     6601 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     6661 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     6721 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     6781 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     6841 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     6901 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     6961 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     7021 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     7081 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     7141 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     7201 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     7261 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     7321 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     7381 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     7441 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     7501 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     7561 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     7621 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     7681 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     7741 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     7801 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     7861 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     7921 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     7981 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     8041 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     8101 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     8161 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     8221 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     8281 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8341 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8401 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     8461 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     8521 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     8581 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     8641 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     8701 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     8761 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     8821 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     8881 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     8941 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     9001 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//