LOCUS       RC215944                8697 bp    DNA     circular  SYN 
DEFINITION  UPF2 (Myc-DDK-tagged)-Human UPF2 regulator of nonsense transcripts homolog (yeast) (UPF2), transcript variant 1
ACCESSION   NM_080599
VERSION     NM_080599.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4854..4945 
                     /label="Myc-DDK"  
     misc_feature    4975..5560 
                     /label="PolyA signal"  
     misc_feature    5032..5051 
                     /label="Restriction Enzyme"  
     misc_feature    5836..6508 
                     /label="ColE1"  
     misc_feature    6938..7732 
                     /label="Kan/Neo"  
     misc_feature    7767..8069 
                     /label="SV40 ori"  
     misc_feature    8155..8632 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4836..4842 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4836 
                     /label="UPF2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCAGCTGAG CGTAAAAAGC CAGCAAGTAT GGAAGAAAAA GACTCTTTAC 
     1081 CAAACAACAA GGAAAAAGAC TGCAGTGAAA GGCGGACAGT GAGCAGCAAG GAGAGGCCAA 
     1141 AAGACGATAT CAAGCTCACT GCCAAGAAGG AGGTCAGCAA GGCCCCTGAA GACAAGAAGA 
     1201 AGAGACTGGA AGATGATAAG AGAAAAAAGG AAGACAAGGA ACGCAAGAAA AAAGACGAAG 
     1261 AAAAGGTGAA GGCAGAGGAA GAATCAAAGA AAAAAGAAGA GGAAGAAAAA AAGAAACATC 
     1321 AAGAGGAAGA GAGAAAGAAG CAAGAAGAGC AGGCCAAACG TCAGCAAGAA GAAGAAGCAG 
     1381 CTGCTCAGAT GAAAGAAAAA GAAGAATCCA TTCAGCTTCA TCAGGAAGCT TGGGAACGAC 
     1441 ATCATTTAAG AAAGGAACTT CGTAGCAAAA ACCAAAATGC TCCGGACAGC CGACCAGAGG 
     1501 AAAACTTCTT CAGCCGCCTC GACTCAAGTT TGAAGAAAAA TACTGCTTTT GTCAAGAAAC 
     1561 TAAAAACTAT TACAGAACAA CAGAGAGACT CCTTGTCCCA TGATTTTAAT GGCCTAAATT 
     1621 TAAGCAAATA CATTGCAGAA GCTGTAGCTT CCATCGTGGA AGCAAAACTA AAAATCTCTG 
     1681 ATGTGAACTG TGCTGTGCAC CTCTGCTCTC TCTTTCACCA GCGTTATGCT GACTTTGCCC 
     1741 CATCACTTCT TCAGGTCTGG AAAAAACATT TTGAAGCAAG GAAAGAGGAG AAAACACCTA 
     1801 ACATCACCAA GTTAAGAACT GATTTGCGTT TTATTGCAGA ATTGACAATA GTTGGGATTT 
     1861 TCACTGACAA GGAAGGTCTT TCCTTAATCT ATGAACAGCT AAAAAATATT ATTAATGCTG 
     1921 ATCGGGAGTC CCACACTCAT GTCTCTGTAG TGATTAGTTT CTGTCGACAT TGTGGAGATG 
     1981 ATATTGCTGG ACTTGTACCA AGGAAAGTAA AGAGTGCTGC AGAGAAGTTT AATTTGAGTT 
     2041 TTCCTCCTAG TGAGATAATT AGTCCAGAGA AACAACAGCC CTTCCAGAAT CTTTTAAAAG 
     2101 AGTACTTTAC GTCTTTGACC AAACACCTGA AAAGGGACCA CAGGGAGCTC CAGAATACTG 
     2161 AGAGACAAAA CAGGCGCATT CTACATTCTA AAGGGGAGCT CAGTGAAGAT AGACATAAAC 
     2221 AGTATGAGGA ATTTGCTATG TCTTACCAGA AGCTGCTGGC AAATTCTCAA TCCTTAGCAG 
     2281 ACCTTTTGGA TGAAAATATG CCAGATCTTC CTCAAGACAA ACCAACACCA GAAGAACATG 
     2341 GGCCTGGAAT TGATATATTC ACACCTGGTA AACCTGGAGA ATATGACTTG GAAGGTGGTA 
     2401 TATGGGAAGA TGAAGATGCT CGGAATTTTT ATGAGAACCT CATTGATTTG AAGGCTTTTG 
     2461 TCCCAGCCAT CTTGTTTAAA GACAATGAAA AAAGTTGTCA GAATAAAGAG TCCAACAAAG 
     2521 ATGATACCAA AGAGGCAAAA GAATCTAAGG AGAATAAGGA GGTATCAAGT CCCGATGATT 
     2581 TGGAACTTGA GTTGGAGAAT CTAGAAATTA ATGATGACAC CTTAGAATTA GAGGGTGGAG 
     2641 ATGAAGCTGA AGATCTTACA AAGAAACTTC TTGATGAACA AGAACAAGAA GATGAGGAAG 
     2701 CCAGCACTGG ATCTCATCTC AAGCTCATAG TAGATGCTTT CCTACAGCAG TTACCCAACT 
     2761 GTGTCAACCG AGATCTGATA GACAAGGCAG CAATGGATTT TTGCATGAAC ATGAACACAA 
     2821 AAGCAAACAG GAAGAAGTTG GTACGGGCAC TCTTCATAGT TCCTAGACAA AGGTTGGATT 
     2881 TGCTACCATT TTATGCAAGA TTGGTTGCTA CATTGCATCC CTGCATGTCT GATGTAGCAG 
     2941 AGGATCTTTG TTCCATGCTG AGGGGGGATT TCAGATTTCA TGTACGGAAA AAGGACCAGA 
     3001 TCAATATTGA AACAAAGAAT AAAACTGTTC GTTTTATAGG AGAACTAACT AAGTTTAAGA 
     3061 TGTTCACCAA AAATGACACA CTGCATTGTT TAAAGATGCT TCTGTCAGAC TTCTCTCATC 
     3121 ACCATATTGA AATGGCATGC ACCCTGCTGG AGACATGTGG ACGGTTTCTT TTCAGATCTC 
     3181 CAGAATCTCA CCTGAGGACC AGTGTACTTT TGGAGCAAAT GATGAGAAAG AAGCAAGCAA 
     3241 TGCATCTTGA TGCGAGATAC GTCACAATGG TAGAGAATGC ATATTACTAC TGCAACCCAC 
     3301 CTCCAGCTGA AAAAACCGTG AAAAAGAAAC GTCCTCCTCT CCAGGAATAT GTCCGGAAAC 
     3361 TTTTGTACAA GGATCTCTCT AAGGTTACCA CCGAGAAGGT TTTGAGACAG ATGCGAAAGC 
     3421 TGCCCTGGCA GGACCAAGAA GTGAAAGACT ATGTTATTTG TTGTATGATA AACATCTGGA 
     3481 ATGTGAAATA TAATAGTATT CATTGTGTAG CCAACCTCTT AGCAGGACTA GTGCTCTACC 
     3541 AAGAGGATGT TGGGATCCAC GTTGTGGATG GAGTGTTAGA AGATATTCGA TTAGGAATGG 
     3601 AGGTTAATCA ACCTAAATTT AATCAGAGGC GCATCAGCAG TGCCAAGTTC TTAGGAGAAC 
     3661 TTTACAATTA CCGAATGGTG GAATCAGCTG TTATTTTCAG AACTCTGTAT TCTTTTACCT 
     3721 CATTTGGTGT TAATCCTGAT GGCTCTCCAA GTTCCCTGGA CCCACCTGAG CATCTTTTCA 
     3781 GAATTAGACT CGTATGCACT ATTCTGGACA CATGTGGCCA GTACTTTGAC AGAGGTTCCA 
     3841 GTAAACGAAA ACTTGATTGT TTCCTTGTAT ATTTTCAGCG TTATGTTTGG TGGAAGAAAA 
     3901 GTTTGGAGGT TTGGACAAAA GACCATCCAT TTCCTATTGA TATAGATTAC ATGATCAGTG 
     3961 ATACACTAGA ACTGCTAAGA CCAAAGATCA AACTCTGTAA TTCTCTGGAA GAATCCATCA 
     4021 GGCAGGTACA AGACTTGGAA CGAGAATTCT TAATAAAACT AGGCCTAGTA AATGACAAAG 
     4081 ACTCAAAAGA TTCTATGACA GAAGGAGAAA ATCTTGAAGA GGATGAAGAA GAAGAAGAAG 
     4141 GTGGGGCTGA AACAGAAGAA CAATCTGGAA ATGAAAGTGA AGTAAATGAG CCAGAAGAAG 
     4201 AGGAGGGTTC TGATAATGAT GATGATGAGG GAGAAGAAGA GGAGGAAGAG AATACAGATT 
     4261 ACCTTACAGA TTCCAATAAG GAAAATGAAA CCGATGAAGA GAATACTGAG GTAATGATTA 
     4321 AAGGCGGTGG ACTTAAGCAT GTACCTTGTG TAGAAGATGA GGACTTCATT CAAGCTCTGG 
     4381 ATAAAATGAT GCTAGAAAAT CTACAGCAAC GAAGTGGTGA ATCTGTTAAA GTGCACCAAC 
     4441 TAGATGTTGC CATTCCTTTG CATCTCAAAA GCCAGCTGAG GAAAGGGCCC CCACTGGGAG 
     4501 GTGGGGAAGG AGAGGCTGAG TCTGCAGACA CAATGCCGTT TGTCATGTTA ACAAGAAAAG 
     4561 GCAATAAACA GCAGTTTAAG ATCCTTAATG TACCCATGTC CTCTCAACTT GCTGCAAATC 
     4621 ACTGGAACCA GCAACAGGCA GAACAAGAAG AGAGGATGAG AATGAAAAAG CTCACACTAG 
     4681 ATATCAATGA ACGGCAAGAA CAAGAAGATT ATCAAGAAAT GTTGCAGTCT CTTGCACAGC 
     4741 GCCCAGCTCC AGCAAACACC AATCGTGAGA GGCGGCCTCG CTACCAACAT CCGAAGGGAG 
     4801 CACCTAATGC AGATCTAATC TTTAAGACTG GTGGGAGGAG ACGTACGCGT ACGCGGCCGC 
     4861 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     4921 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4981 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     5041 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     5101 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     5161 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     5221 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     5281 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     5341 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     5401 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     5461 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     5521 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5581 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     5641 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5701 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5761 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5821 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     5881 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     5941 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     6001 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     6061 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     6121 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     6181 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     6241 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     6301 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     6361 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     6421 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     6481 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     6541 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     6601 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6661 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6721 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6781 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6841 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     6901 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     6961 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     7021 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     7081 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     7141 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     7201 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     7261 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     7321 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     7381 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     7441 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     7501 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     7561 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     7621 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7681 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7741 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7801 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7861 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     7921 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7981 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     8041 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     8101 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     8161 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     8221 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     8281 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     8341 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     8401 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     8461 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     8521 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     8581 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     8641 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//