LOCUS       RC215918                7149 bp    DNA     circular  SYN 
DEFINITION  FAM22F (Myc-DDK-tagged)-Human family with sequence similarity 22, member F (FAM22F)
ACCESSION   NM_017561
VERSION     NM_017561.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3306..3397 
                     /label="Myc-DDK"  
     misc_feature    3427..4012 
                     /label="PolyA signal"  
     misc_feature    3484..3503 
                     /label="Restriction Enzyme"  
     misc_feature    4288..4960 
                     /label="ColE1"  
     misc_feature    5390..6184 
                     /label="Kan/Neo"  
     misc_feature    6219..6521 
                     /label="SV40 ori"  
     misc_feature    6607..7084 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3288..3294 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3288 
                     /label="NUTM2F"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCTTCAAAT GGAGCATACC CAGTGCTGGG ACCCGGCGTG ACCGTGAACC 
     1081 CTGGCACCTC CCTGTCTGTG TTCACGGCTC TGCCCTTTGC CACACCCGCT CCCGGCCCAG 
     1141 CACACAGGCC GCCCCTCGTG ACTGCAGTGG TTCCTCCAGC CGGCCCTCTG GTGCTCTCTG 
     1201 CCTTCCCCAG CACCCCTCTA GTGGCAGGAC AGGATGGCCG CGGCCCGAGT GGGGCCGGGG 
     1261 CTTCCAACGT CTTTGTCCAG ATGAGGACAG AAGTGGGGCC TGTGAAGCCC CCTCAGGCAC 
     1321 AGACCTTGAT CCTAACTCAG GCCCCCCTCG TCTGGCAGGC TCCAGGCACC CTCTGTGGAG 
     1381 GTGTCATGTG TCCACCTCCC CTACTCCTGG CAGCTGCTCC TGGGGTGCCC GTTACCTCTG 
     1441 CCCAGGTGGT TGGGGGCACC CAGGCCTGTG AGGGAGGCTG GTCCCATGGC CTTCCTCTTC 
     1501 CACCACCACC ACCGGCTGCC CAGGTGGCCC CCATCGTGTC CCCAGGGAAC GCTAGGCCAT 
     1561 GGCCACAAGG GGCTCATGGA GAGGGCAGCC TGGCTCCCTC CCAGGCCAAG GCCCGGCCGG 
     1621 ACGACTCCTG TAAACCCAAG AGTGTCTATG AGAACTTCCG ACTCTGGCAG CACTACAAGC 
     1681 CCCTGGCCCG GAGGCACCTT CCCCAGAGTC CTGACACTGA AGCGCTTTCC TGCTTCCTCA 
     1741 TCCCAGTTCT CCGATCCCTG GCCCGGCGGA AGCCCACCAT GACGCTGGAG GAGGGACTGT 
     1801 GGCAGGCCAT GCGGGAATGG CAGCACACGA GCAACTTTGA CCGGATGATC TTCTACGAGA 
     1861 TGGCGGAAAA GTTCCTGGAA TTTGAGGCTG AGGAGGAGAT GCAGATTCAG AAATCGCAGT 
     1921 GGATGAAGGG GCCCCAGAGC CTGCCTCCTC CAGCCCCGCC GAGGCTTGAA CCTCGAGGAC 
     1981 CCCCTGCCCC TGAGGTGGTC AAGCAGCCAG TGTACCTTCC CAGCAAGGAT GGCCCCAAGG 
     2041 CCCCGACTGC CTGCCTGCCA CCACCCAGGC CCCAGAGGCC AGCGGAGACC AAGGCCCACC 
     2101 TGCCACCACC CAGGCCCCAG AGGCCAGCGG AGACCAACGC CCACCTGCCA CCACCCAGGC 
     2161 CCCAGAGGCC AGCGGAGACC AAGGTCCCTG AGGAGATCCC CCCTGAAGTG GTGCAGGAGT 
     2221 ATGTGGACAT CATGGAGGAG CTGCTGGGGT CTCACCCTGG GGACACAGGG GAGCCTGAGG 
     2281 GACAACGGGA AAAGGGCAAA GTGGAGCAGC CGCAGGAAGA GGACGGGATA ACCTCAGACC 
     2341 CGGGCCTCCT GAGCTACATT GACAAGCTGT GTTCCCAGGA AGACTTTGTC ACCAAGGTGG 
     2401 AGGCCGTCAT TCACCCCCGA TTCCTGGAAG AATTGCTTTC CCCAGATCCA CAGATGGATT 
     2461 TCTTGGCCCT AAGCCAGGAG CTGGAGCAGG AGGAAGGACT CACCCTTGCC CAGCTAGTGG 
     2521 AGAAGCGCCT CCTGTCCTTG AAGGAGAAAG GGTGCGGGAG GGCAGCCCCT CGACATGGCA 
     2581 CGGCCAGGTT GGACTCAAGT CCTTCTGAGT TTGCAGCTGG CCAAGAAGCA GCGAGAGAGG 
     2641 TCCCTGACCC CCAACAAAGG GTCAGCGTGG AAACCTCCCC ACCCCAGACA GCTGCCCAGG 
     2701 ACCCTCAGGG ACAGGGCAGA GTGCGCACTG GCATGGCCAG GTCCGAAGAC CCTGCTGTGC 
     2761 TTTTGGGATG TCAGGATTCC CCCAGGCTGA AGGCTGTCCG GCCAACCTCT CCTCCCCAGG 
     2821 ACCACAGACC CACCTGCCCC GGCCTGGGGA CCAAGGACGC CTTGGGTCTC CCTGGAGAGT 
     2881 CTCCTGTCAA GGAGTCACAT GGGCTGGCTA AGGGGTCAAG TGAGGAGACG GAACTCCCTG 
     2941 GCATGGTCTA TGTCGTGGGT TCCCACCACA GGCTGAGGCC CTGGAGGCTG TCCCAGAGCC 
     3001 CTGTCCCTTC CTCGGGCCTT CTCAGCCCAG GAGGGAGAGG ACCCCAGGGA GCTCTTCAGT 
     3061 CTCCATCTGC TCAGAAAAGA GGCCTCAGCC CATCACCTTC TCCTGCCAGC AAGTCCAAGA 
     3121 AGCGACCTCT CTTTGGAAGC CCATCCCCTG CTGAAAAGAC ACCGCACCCA GGGCCTGGGC 
     3181 TCAGGGTCTC TGGGGAGCAA TCCCTGGCTT GGGGGCTGGG TGGCCCCTCA CAGTCTCAAA 
     3241 AGAGAAAGGG TGACCCCTTG GCCTCCAGGA GGAAGAAGAA GCGGCATTGT AGCCAGACGC 
     3301 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     3361 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     3421 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     3481 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     3541 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     3601 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     3661 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     3721 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     3781 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     3841 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     3901 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     3961 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     4021 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     4081 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     4141 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     4201 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     4261 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     4321 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     4381 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     4441 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     4501 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     4561 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     4621 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     4681 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     4741 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     4801 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     4861 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     4921 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     4981 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     5041 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     5101 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     5161 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     5221 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     5281 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     5341 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     5401 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     5461 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     5521 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     5581 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     5641 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     5701 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     5761 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     5821 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     5881 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     5941 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     6001 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     6061 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     6121 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     6181 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     6241 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     6301 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     6361 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     6421 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     6481 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     6541 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     6601 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     6661 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     6721 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     6781 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     6841 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     6901 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     6961 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     7021 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     7081 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     7141 GCGAATTTT
//