LOCUS       RC215879L1                8661 bp    DNA     circular  SYN 
DEFINITION  PRKG2 (Myc-DDK-tagged)-Human protein kinase, cGMP-dependent, type II (PRKG2)
ACCESSION   NM_006259
VERSION     null
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    6952..7132 
                     /label="3'LTR"  
     misc_feature    833..1013 
                     /label="5'LTR"  
     misc_feature    7860..8519 
                     /label="CAT(Cam-r)"  
     misc_feature    2620..3234 
                     /label="CMV promoter"  
     misc_feature    7291..7859 
                     /label="ColE1"  
     misc_feature    5827..5847 
                     /label="LR50 primer"  
     misc_feature    5624..5695 
                     /label="Myc-DDK"  
     misc_feature    3252..3271 
                     /label="T7 promoter"  
     misc_feature    3221..3237 
                     /label="V2 primer"  
     misc_feature    5836..6427 
                     /label="WPRE"  
     misc_feature    3320..3328 
                     /label="Restriction Enzyme"  
     misc_feature    5606..5612 
                     /label="Restriction Enzyme"  
     misc_feature    3320..5606 
                     /label="PRKG2"  
ORIGIN                      
        1 GTCGACGGAT CGGGAGATCT CCCGATCCCC TATGGTGCAC TCTCAGTACA ATCTGCTCTG 
       61 ATGCCGCATA GTTAAGCCAG TATCTGCTCC CTGCTTGTGT GTTGGAGGTC GCTGAGTAGT 
      121 GCGCGAGCAA AATTTAAGCT ACAACAAGGC AAGGCTTGAC CGACAATTGC ATGAAGAATC 
      181 TGCTTAGGGT TAGGCGTTTT GCGCTGCTTC GCGATGTACG GGCCAGATAT CGCGTTGACA 
      241 TTGATTATTG ACTAGTTATT AATAGTAATC AATTACGGGG TCATTAGTTC ATAGCCCATA 
      301 TATGGAGTTC CGCGTTACAT AACTTACGGT AAATGGCCCG CCTGGCTGAC CGCCCAACGA 
      361 CCCCCGCCCA TTGACGTCAA TAATGACGTA TGTTCCCATA GTAACGCCAA TAGGGACTTT 
      421 CCATTGACGT CAATGGGTGG AGTATTTACG GTAAACTGCC CACTTGGCAG TACATCAAGT 
      481 GTATCATATG CCAAGTACGC CCCCTATTGA CGTCAATGAC GGTAAATGGC CCGCCTGGCA 
      541 TTATGCCCAG TACATGACCT TATGGGACTT TCCTACTTGG CAGTACATCT ACGTATTAGT 
      601 CATCGCTATT ACCATGGTGA TGCGGTTTTG GCAGTACATC AATGGGCGTG GATAGCGGTT 
      661 TGACTCACGG GGATTTCCAA GTCTCCACCC CATTGACGTC AATGGGAGTT TGTTTTGGCA 
      721 CCAAAATCAA CGGGACTTTC CAAAATGTCG TAACAACTCC GCCCCATTGA CGCAAATGGG 
      781 CGGTAGGCGT GTACGGTGGG AGGTCTATAT AAGCAGCGCG TTTTGCCTGT ACTGGGTCTC 
      841 TCTGGTTAGA CCAGATCTGA GCCTGGGAGC TCTCTGGCTA ACTAGGGAAC CCACTGCTTA 
      901 AGCCTCAATA AAGCTTGCCT TGAGTGCTTC AAGTAGTGTG TGCCCGTCTG TTGTGTGACT 
      961 CTGGTAACTA GAGATCCCTC AGACCCTTTT AGTCAGTGTG GAAAATCTCT AGCAGTGGCG 
     1021 CCCGAACAGG GACTTGAAAG CGAAAGGGAA ACCAGAGGAG CTCTCTCGAC GCAGGACTCG 
     1081 GCTTGCTGAA GCGCGCACGG CAAGAGGCGA GGGGCGGCGA CTGGTGAGTA CGCCAAAAAT 
     1141 TTTGACTAGC GGAGGCTAGA AGGAGAGAGA TGGGTGCGAG AGCGTCAGTA TTAAGCGGGG 
     1201 GAGAATTAGA TCGCGATGGG AAAAAATTCG GTTAAGGCCA GGGGGAAAGA AAAAATATAA 
     1261 ATTAAAACAT ATAGTATGGG CAAGCAGGGA GCTAGAACGA TTCGCAGTTA ATCCTGGCCT 
     1321 GTTAGAAACA TCAGAAGGCT GTAGACAAAT ACTGGGACAG CTACAACCAT CCCTTCAGAC 
     1381 AGGATCAGAA GAACTTAGAT CATTATATAA TACAGTAGCA ACCCTCTATT GTGTGCATCA 
     1441 AAGGATAGAG ATAAAAGACA CCAAGGAAGC TTTAGACAAG ATAGAGGAAG AGCAAAACAA 
     1501 AAGTAAGACC ACCGCACAGC AAGCGGCCGG CCGCTGATCT TCAGACCTGG AGGAGGAGAT 
     1561 ATGAGGGACA ATTGGAGAAG TGAATTATAT AAATATAAAG TAGTAAAAAT TGAACCATTA 
     1621 GGAGTAGCAC CCACCAAGGC AAAGAGAAGA GTGGTGCAGA GAGAAAAAAG AGCAGTGGGA 
     1681 ATAGGAGCTT TGTTCCTTGG GTTCTTGGGA GCAGCAGGAA GCACTATGGG CGCAGCGTCA 
     1741 ATGACGCTGA CGGTACAGGC CAGACAATTA TTGTCTGGTA TAGTGCAGCA GCAGAACAAT 
     1801 TTGCTGAGGG CTATTGAGGC GCAACAGCAT CTGTTGCAAC TCACAGTCTG GGGCATCAAG 
     1861 CAGCTCCAGG CAAGAATCCT GGCTGTGGAA AGATACCTAA AGGATCAACA GCTCCTGGGG 
     1921 ATTTGGGGTT GCTCTGGAAA ACTCATTTGC ACCACTGCTG TGCCTTGGAA TGCTAGTTGG 
     1981 AGTAATAAAT CTCTGGAACA GATTTGGAAT CACACGACCT GGATGGAGTG GGACAGAGAA 
     2041 ATTAACAATT ACACAAGCTT AATACACTCC TTAATTGAAG AATCGCAAAA CCAGCAAGAA 
     2101 AAGAATGAAC AAGAATTATT GGAATTAGAT AAATGGGCAA GTTTGTGGAA TTGGTTTAAC 
     2161 ATAACAAATT GGCTGTGGTA TATAAAATTA TTCATAATGA TAGTAGGAGG CTTGGTAGGT 
     2221 TTAAGAATAG TTTTTGCTGT ACTTTCTATA GTGAATAGAG TTAGGCAGGG ATATTCACCA 
     2281 TTATCGTTTC AGACCCACCT CCCAACCCCG AGGGGACCCG ACAGGCCCGA AGGAATAGAA 
     2341 GAAGAAGGTG GAGAGAGAGA CAGAGACAGA TCCATTCGAT TAGTGAACGG ATCGGCACTG 
     2401 CGTGCGCCAA TTCTGCAGAC AAATGGCAGT ATTCATCCAC AATTTTAAAA GAAAAGGGGG 
     2461 GATTGGGGGG TACAGTGCAG GGGAAAGAAT AGTAGACATA ATAGCAACAG ACATACAAAC 
     2521 TAAAGAATTA CAAAAACAAA TTACAAAAAT TCAAAATTTT CGGGTTTATT ACAGGGACAG 
     2581 CAGAGATCCA GTTTGGTTAG TACCGGGCCC GCTCTAGACA TGTCCAATAT GACCGCCATG 
     2641 TTGACATTGA TTATTGACTA GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG 
     2701 CCCATATATG GAGTTCCGCG TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC 
     2761 CAACGACCCC CGCCCATTGA CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG 
     2821 GACTTTCCAT TGACGTCAAT GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA 
     2881 TCAAGTGTAT CATATGCCAA GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC 
     2941 CTGGCATTAT GCCCAGTACA TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT 
     3001 ATTAGTCATC GCTATTACCA TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA 
     3061 GCGGTTTGAC TCACGGGGAT TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT 
     3121 TTGGCACCAA AATCAACGGG ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA 
     3181 AATGGGCGGT AGGCGTGTAC GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG 
     3241 TCAGAATTTT GTAATACGAC TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT 
     3301 ACCGAGGAGA TCTGCCGCCG CGATCGCCAT GGGAAATGGT TCAGTGAAAC CTAAACATTC 
     3361 TAAGCACCCA GATGGACACT CTGGGAACCT CACCACTGAT GCTCTGCGGA ACAAGGTGAC 
     3421 AGAGCTGGAG AGAGAGTTGA GGAGGAAGGA TGCTGAGATC CAGGAGCGGG AGTACCATTT 
     3481 GAAGGAGCTG CGGGAGCAGC TGTCGAAGCA GACTGTGGCC ATTGCTGAAC TCACAGAGGA 
     3541 GCTCCAGAAC AAGTGCATCC AGCTGAACAA GCTGCAGGAT GTGGTGCATA TGCAGGGAGG 
     3601 AAGCCCGCTT CAGGCCTCTC CAGATAAAGT GCCTCTTGAG GTCCACCGGA AGACCTCTGG 
     3661 ATTGGTCTCT CTCCATAGCA GGAGGGGAGC AAAGGCTGGC GTGTCTGCTG AGCCAACAAC 
     3721 CCGGACCTAT GACCTGAACA AACCCCCTGA ATTTTCCTTT GAGAAAGCAA GAGTCAGAAA 
     3781 AGACTCCAGT GAGAAGAAGC TCATTACAGA TGCCCTTAAT AAAAATCAGT TTCTGAAAAG 
     3841 ACTGGATCCT CAGCAGATCA AAGACATGGT GGAATGCATG TATGGGAGAA ACTATCAGCA 
     3901 AGGGAGTTAC ATTATTAAGC AAGGAGAACC AGGAAACCAT ATCTTTGTGC TGGCAGAGGG 
     3961 TCGACTAGAG GTGTTCCAAG GGGAGAAATT GCTGTCCTCC ATCCCTATGT GGACCACATT 
     4021 TGGGGAGCTT GCCATTTTAT ACAATTGTAC AAGGACTGCC TCTGTGAAAG CTATTACCAA 
     4081 TGTTAAAACA TGGGCACTAG ATCGAGAGGT ATTCCAGAAT ATAATGAGGA GGACAGCCCA 
     4141 AGCTAGAGAT GAACAATACA GAAACTTCCT CAGAAGTGTA TCCTTGCTGA AGAATTTACC 
     4201 TGAAGATAAA TTAACCAAGA TCATTGACTG CTTGGAAGTG GAATACTATG ACAAAGGAGA 
     4261 TTACATCATT AGAGAGGGCG AGGAAGGAAG TACCTTTTTC ATTTTGGCAA AAGGAAAGGT 
     4321 AAAAGTAACA CAGAGCACAG AAGGCCATGA TCAACCACAG CTGATAAAAA CACTGCAGAA 
     4381 AGGAGAATAC TTTGGAGAAA AAGCTCTTAT CAGTGATGAT GTCAGGTCAG CTAACATTAT 
     4441 TGCTGAAGAA AATGATGTTG CATGCCTGGT TATAGATCGA GAAACATTCA ACCAAACTGT 
     4501 CGGTACATTT GAAGAGCTGC AAAAATACCT TGAAGGATAT GTGGCAAACC TGAACCGTGA 
     4561 TGATGAAAAA AGACATGCGA AGCGGTCCAT GTCTAACTGG AAGCTGTCCA AAGCACTCTC 
     4621 TCTGGAAATG ATTCAGCTGA AGGAGAAGGT GGCCAGATTT TCCTCATCAT CCCCATTCCA 
     4681 GAACCTTGAG ATTATTGCAA CACTGGGCGT TGGTGGGTTC GGAAGAGTTG AGCTTGTTAA 
     4741 AGTAAAAAAT GAGAATGTTG CTTTTGCTAT GAAGTGTATA AGGAAGAAGC ACATAGTTGA 
     4801 CACCAAGCAG CAGGAGCATG TCTACTCAGA GAAGAGGATC CTAGAGGAGC TGTGCTCTCC 
     4861 ATTCATTGTG AAATTATATC GTACTTTCAA GGACAATAAG TATGTATACA TGCTTCTGGA 
     4921 GGCCTGCTTA GGTGGGGAGC TCTGGAGTAT ATTAAGGGAC AGAGGCAGCT TTGATGAACC 
     4981 CACCTCCAAA TTCTGCGTTG CTTGTGTGAC AGAAGCATTT GATTACCTGC ATCGACTAGG 
     5041 TATTATCTAC AGAGACTTGA AACCAGAAAA CTTAATTCTA GATGCTGAGG GTTACCTTAA 
     5101 ATTGGTTGAC TTTGGATTTG CGAAGAAAAT AGGGTCTGGA CAGAAAACAT GGACATTCTG 
     5161 TGGGACTCCA GAATATGTAG CTCCTGAAGT CATTCTCAAC AAGGGACATG ACTTCAGTGT 
     5221 GGATTTCTGG TCACTGGGAA TTCTAGTGTA TGAGCTCCTA ACGGGCAACC CACCCTTTTC 
     5281 TGGGGTTGAC CAAATGATGA CCTACAATTT GATTCTCAAA GGAATTGAAA AAATGGATTT 
     5341 TCCCAGGAAG ATAACACGAC GACCTGAGGA TTTGATTCGG AGGCTTTGCA GGCAAAATCC 
     5401 AACAGAAAGG CTGGGAAATC TGAAGAATGG AATAAATGAC ATTAAGAAAC ACAGGTGGTT 
     5461 AAATGGTTTT AATTGGGAGG GACTGAAAGC ACGGAGCCTT CCATCACCTT TGCAAAGAGA 
     5521 GCTCAAGGGA CCCATAGATC ACAGCTACTT TGACAAATAT CCTCCTGAAA AGGGAATGCC 
     5581 TCCAGATGAG CTATCAGGCT GGGATAAAGA CTTCACGCGT ACGCGGCCGC TCGAGCAGAA 
     5641 ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA 
     5701 TAAGGTTTAA ACGGCCGGCC GCGGTCTGTA CAAGTAGGAT TCGTCGAGGG ACCTAATAAC 
     5761 TTCGTATAGC ATACATTATA CGAAGTTATA CATGTTTAAG GGTTCCGGTT CCACTAGGTA 
     5821 CAATTCGATA TCAAGCTTAT CGATAATCAA CCTCTGGATT ACAAAATTTG TGAAAGATTG 
     5881 ACTGGTATTC TTAACTATGT TGCTCCTTTT ACGCTATGTG GATACGCTGC TTTAATGCCT 
     5941 TTGTATCATG CTATTGCTTC CCGTATGGCT TTCATTTTCT CCTCCTTGTA TAAATCCTGG 
     6001 TTGCTGTCTC TTTATGAGGA GTTGTGGCCC GTTGTCAGGC AACGTGGCGT GGTGTGCACT 
     6061 GTGTTTGCTG ACGCAACCCC CACTGGTTGG GGCATTGCCA CCACCTGTCA GCTCCTTTCC 
     6121 GGGACTTTCG CTTTCCCCCT CCCTATTGCC ACGGCGGAAC TCATCGCCGC CTGCCTTGCC 
     6181 CGCTGCTGGA CAGGGGCTCG GCTGTTGGGC ACTGACAATT CCGTGGTGTT GTCGGGGAAA 
     6241 TCATCGTCCT TTCCTTGGCT GCTCGCCTGT GTTGCCACCT GGATTCTGCG CGGGACGTCC 
     6301 TTCTGCTACG TCCCTTCGGC CCTCAATCCA GCGGACCTTC CTTCCCGCGG CCTGCTGCCG 
     6361 GCTCTGCGGC CTCTTCCGCG TCTTCGCCTT CGCCCTCAGA CGAGTCGGAT CTCCCTTTGG 
     6421 GCCGCCTCCC CGCATCGATA CCGTCGACCT CGATCGAGAC CTAGAAAAAC ATGGAGCAAT 
     6481 CACAAGTAGC AATACAGCAG CTACCAATGC TGATTGTGCC TGGCTAGAAG CACAAGAGGA 
     6541 GGAGGAGGTG GGTTTTCCAG TCACACCTCA GGTACCTTTA AGACCAATGA CTTACAAGGC 
     6601 AGCTGTAGAT CTTAGCCACT TTTTAAAAGA AAAGGGGGGA CTGGAAGGGC TAATTCACTC 
     6661 CCAACGAAGA CAAGATATCC TTGATCTGTG GATCTACCAC ACACAAGGCT ACTTCCCTGA 
     6721 TTGGCAGAAC TACACACCAG GGCCAGGGAT CAGATATCCA CTGACCTTTG GATGGTGCTA 
     6781 CAAGCTAGTA CCAGTTGAGC AAGAGAAGGT AGAAGAAGCC AATGAAGGAG AGAACACCCG 
     6841 CTTGTTACAC CCTGTGAGCC TGCATGGGAT GGATGACCCG GAGAGAGAAG TATTAGAGTG 
     6901 GAGGTTTGAC AGCCGCCTAG CATTTCATCA CATGGCCCGA GAGCTGCATC CGGACTGTAC 
     6961 TGGGTCTCTC TGGTTAGACC AGATCTGAGC CTGGGAGCTC TCTGGCTAAC TAGGGAACCC 
     7021 ACTGCTTAAG CCTCAATAAA GCTTGCCTTG AGTGCTTCAA GTAGTGTGTG CCCGTCTGTT 
     7081 GTGTGACTCT GGTAACTAGA GATCCCTCAG ACCCTTTTAG TCAGTGTGGA AAATCTCTAG 
     7141 CAGCATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     7201 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     7261 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     7321 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     7381 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     7441 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     7501 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     7561 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     7621 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     7681 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     7741 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     7801 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     7861 GGTCATGATT ACGCCCCGCC CTGCCACTCA TCGCAGTACT GTTGTAATTC ATTAAGCATT 
     7921 CTGCCGACAT GGAAGCCATC ACAAACGGCA TGATGAACCT GAATCGCCAG CGGCATCAGC 
     7981 ACCTTGTCGC CTTGCGTATA ATATTTGCCC ATGGTGAAAA CGGGGGCGAA GAAGTTGTCC 
     8041 ATATTGGCCA CGTTTAAATC AAAACTGGTG AAACTCACCC AGGGATTGGC TGAGACGAAA 
     8101 AACATATTCT CAATAAACCC TTTAGGGAAA TAGGCCAGGT TTTCACCGTA ACACGCCACA 
     8161 TCTTGCGAAT ATATGTGTAG AAACTGCCGG AAATCGTCGT GGTATTCACT CCAGAGCGAT 
     8221 GAAAACGTTT CAGTTTGCTC ATGGAAAACG GTGTAACAAG GGTGAACACT ATCCCATATC 
     8281 ACCAGCTCAC CGTCTTTCAT TGCCATACGG AACTCCGGAT GAGCATTCAT CAGGCGGGCA 
     8341 AGAATGTGAA TAAAGGCCGG ATAAAACTTG TGCTTATTTT TCTTTACGGT CTTTAAAAAG 
     8401 GCCGTAATAT CCAGCTGAAC GGTCTGGTTA TAGGTACATT GAGCAACTGA CTGAAATGCC 
     8461 TCAAAATGTT CTTTACGATG CCATTGGGAT ATATCAACGG TGGTATATCC AGTGATTTTT 
     8521 TTCTCCATAC TCTTCCTTTT TCAATATTAT TGAAGCATTT ATCAGGGTTA TTGTCTCATG 
     8581 AGCGGATACA TATTTGAATG TATTTAGAAA AATAAACAAA TAGGGGTCCC GCGCACATTT 
     8641 CCCCGAAAAG TGCCACCTGA C
//