LOCUS       RC215879                7167 bp    DNA     circular  SYN 
DEFINITION  PRKG2 (Myc-DDK-tagged)-Human protein kinase, cGMP-dependent, type II (PRKG2)
ACCESSION   NM_006259
VERSION     NM_006259.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3324..3415 
                     /label="Myc-DDK"  
     misc_feature    3445..4030 
                     /label="PolyA signal"  
     misc_feature    3502..3521 
                     /label="Restriction Enzyme"  
     misc_feature    4306..4978 
                     /label="ColE1"  
     misc_feature    5408..6202 
                     /label="Kan/Neo"  
     misc_feature    6237..6539 
                     /label="SV40 ori"  
     misc_feature    6625..7102 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3306..3312 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3306 
                     /label="PRKG2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGAAATGGT TCAGTGAAAC CTAAACATTC TAAGCACCCA GATGGACACT 
     1081 CTGGGAACCT CACCACTGAT GCTCTGCGGA ACAAGGTGAC AGAGCTGGAG AGAGAGTTGA 
     1141 GGAGGAAGGA TGCTGAGATC CAGGAGCGGG AGTACCATTT GAAGGAGCTG CGGGAGCAGC 
     1201 TGTCGAAGCA GACTGTGGCC ATTGCTGAAC TCACAGAGGA GCTCCAGAAC AAGTGCATCC 
     1261 AGCTGAACAA GCTGCAGGAT GTGGTGCATA TGCAGGGAGG AAGCCCGCTT CAGGCCTCTC 
     1321 CAGATAAAGT GCCTCTTGAG GTCCACCGGA AGACCTCTGG ATTGGTCTCT CTCCATAGCA 
     1381 GGAGGGGAGC AAAGGCTGGC GTGTCTGCTG AGCCAACAAC CCGGACCTAT GACCTGAACA 
     1441 AACCCCCTGA ATTTTCCTTT GAGAAAGCAA GAGTCAGAAA AGACTCCAGT GAGAAGAAGC 
     1501 TCATTACAGA TGCCCTTAAT AAAAATCAGT TTCTGAAAAG ACTGGATCCT CAGCAGATCA 
     1561 AAGACATGGT GGAATGCATG TATGGGAGAA ACTATCAGCA AGGGAGTTAC ATTATTAAGC 
     1621 AAGGAGAACC AGGAAACCAT ATCTTTGTGC TGGCAGAGGG TCGACTAGAG GTGTTCCAAG 
     1681 GGGAGAAATT GCTGTCCTCC ATCCCTATGT GGACCACATT TGGGGAGCTT GCCATTTTAT 
     1741 ACAATTGTAC AAGGACTGCC TCTGTGAAAG CTATTACCAA TGTTAAAACA TGGGCACTAG 
     1801 ATCGAGAGGT ATTCCAGAAT ATAATGAGGA GGACAGCCCA AGCTAGAGAT GAACAATACA 
     1861 GAAACTTCCT CAGAAGTGTA TCCTTGCTGA AGAATTTACC TGAAGATAAA TTAACCAAGA 
     1921 TCATTGACTG CTTGGAAGTG GAATACTATG ACAAAGGAGA TTACATCATT AGAGAGGGCG 
     1981 AGGAAGGAAG TACCTTTTTC ATTTTGGCAA AAGGAAAGGT AAAAGTAACA CAGAGCACAG 
     2041 AAGGCCATGA TCAACCACAG CTGATAAAAA CACTGCAGAA AGGAGAATAC TTTGGAGAAA 
     2101 AAGCTCTTAT CAGTGATGAT GTCAGGTCAG CTAACATTAT TGCTGAAGAA AATGATGTTG 
     2161 CATGCCTGGT TATAGATCGA GAAACATTCA ACCAAACTGT CGGTACATTT GAAGAGCTGC 
     2221 AAAAATACCT TGAAGGATAT GTGGCAAACC TGAACCGTGA TGATGAAAAA AGACATGCGA 
     2281 AGCGGTCCAT GTCTAACTGG AAGCTGTCCA AAGCACTCTC TCTGGAAATG ATTCAGCTGA 
     2341 AGGAGAAGGT GGCCAGATTT TCCTCATCAT CCCCATTCCA GAACCTTGAG ATTATTGCAA 
     2401 CACTGGGCGT TGGTGGGTTC GGAAGAGTTG AGCTTGTTAA AGTAAAAAAT GAGAATGTTG 
     2461 CTTTTGCTAT GAAGTGTATA AGGAAGAAGC ACATAGTTGA CACCAAGCAG CAGGAGCATG 
     2521 TCTACTCAGA GAAGAGGATC CTAGAGGAGC TGTGCTCTCC ATTCATTGTG AAATTATATC 
     2581 GTACTTTCAA GGACAATAAG TATGTATACA TGCTTCTGGA GGCCTGCTTA GGTGGGGAGC 
     2641 TCTGGAGTAT ATTAAGGGAC AGAGGCAGCT TTGATGAACC CACCTCCAAA TTCTGCGTTG 
     2701 CTTGTGTGAC AGAAGCATTT GATTACCTGC ATCGACTAGG TATTATCTAC AGAGACTTGA 
     2761 AACCAGAAAA CTTAATTCTA GATGCTGAGG GTTACCTTAA ATTGGTTGAC TTTGGATTTG 
     2821 CGAAGAAAAT AGGGTCTGGA CAGAAAACAT GGACATTCTG TGGGACTCCA GAATATGTAG 
     2881 CTCCTGAAGT CATTCTCAAC AAGGGACATG ACTTCAGTGT GGATTTCTGG TCACTGGGAA 
     2941 TTCTAGTGTA TGAGCTCCTA ACGGGCAACC CACCCTTTTC TGGGGTTGAC CAAATGATGA 
     3001 CCTACAATTT GATTCTCAAA GGAATTGAAA AAATGGATTT TCCCAGGAAG ATAACACGAC 
     3061 GACCTGAGGA TTTGATTCGG AGGCTTTGCA GGCAAAATCC AACAGAAAGG CTGGGAAATC 
     3121 TGAAGAATGG AATAAATGAC ATTAAGAAAC ACAGGTGGTT AAATGGTTTT AATTGGGAGG 
     3181 GACTGAAAGC ACGGAGCCTT CCATCACCTT TGCAAAGAGA GCTCAAGGGA CCCATAGATC 
     3241 ACAGCTACTT TGACAAATAT CCTCCTGAAA AGGGAATGCC TCCAGATGAG CTATCAGGCT 
     3301 GGGATAAAGA CTTCACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     3361 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     3421 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     3481 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     3541 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     3601 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     3661 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     3721 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     3781 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     3841 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     3901 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     3961 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     4021 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     4081 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     4141 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     4201 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     4261 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     4321 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     4381 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     4441 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     4501 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     4561 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     4621 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     4681 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     4741 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     4801 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     4861 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     4921 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     4981 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     5041 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     5101 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     5161 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     5221 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     5281 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     5341 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     5401 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     5461 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     5521 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     5581 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     5641 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     5701 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     5761 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     5821 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     5881 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     5941 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     6001 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     6061 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     6121 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     6181 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     6241 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     6301 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     6361 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     6421 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     6481 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     6541 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     6601 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     6661 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     6721 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     6781 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     6841 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     6901 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     6961 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     7021 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     7081 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     7141 ATTTAACAAA AATTTAACGC GAATTTT
//