LOCUS       RC215853                8538 bp    DNA     circular  SYN 
DEFINITION  FKBP15 (Myc-DDK-tagged)-Human FK506 binding protein 15, 133kDa (FKBP15)
ACCESSION   NM_015258
VERSION     NM_015258.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4695..4786 
                     /label="Myc-DDK"  
     misc_feature    4816..5401 
                     /label="PolyA signal"  
     misc_feature    4873..4892 
                     /label="Restriction Enzyme"  
     misc_feature    5677..6349 
                     /label="ColE1"  
     misc_feature    6779..7573 
                     /label="Kan/Neo"  
     misc_feature    7608..7910 
                     /label="SV40 ori"  
     misc_feature    7996..8473 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4677..4683 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4677 
                     /label="FKBP15"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTTCGGTGCG GGGGACGAGG ACGACACCGA TTTCCTCTCG CCGAGCGGCG 
     1081 GTGCCAGATT GGCCTCACTT TTTGGACTGG ATCAGGCAGC TGCTGGCCAT GGAAATGAAT 
     1141 TTTTCCAGTA CACAGCCCCA AAACAGCCTA AGAAAGGCCA GGGAACGGCA GCAACAGGAA 
     1201 ATCAGGCAAC ACCAAAAACA GCACCAGCCA CCATGAGCAC TCCCACAATA CTGGTCGCAA 
     1261 CAGCAGTCCA TGCATATCGA TACACAAATG GTCAATATGT AAAGCAGGGC AAATTTGGTG 
     1321 CTGCAGTTCT GGGGAACCAC ACAGCCAGAG AGTATAGGAT TCTTCTTTAT ATCAGTCAAC 
     1381 AACAGCCAGT TACGGTTGCT AGGATTCATG TGAACTTTGA GCTAATGGTT CGGCCCAATA 
     1441 ACTATAGCAC CTTTTATGAT GACCAGAGAC AGAACTGGTC CATCATGTTT GAGTCGGAAA 
     1501 AGGCTGCTGT GGAGTTCAAT AAGCAGGTGT GCATTGCTAA GTGCAACAGT ACCTCTTCCC 
     1561 TGGATGCAGT GCTCTCCCAG GACCTCATTG TGGCAGACGG CCCTGCTGTA GAAGTTGGAG 
     1621 ATTCTTTGGA AGTGGCCTAT ACCGGCTGGC TCTTTCAGAA TCATGTGCTG GGCCAGGTTT 
     1681 TCGACTCCAC TGCTAACAAA GATAAGTTGC TTCGCTTGAA GTTAGGATCA GGAAAAGTCA 
     1741 TCAAGGGCTG GGAGGATGGA ATGCTGGGCA TGAAAAAAGG AGGAAAGCGA TTGCTTATTG 
     1801 TCCCTCCAGC CTGTGCTGTT GGCTCAGAAG GGGTAATAGG CTGGACTCAA GCAACGGACT 
     1861 CGATCCTGGT GTTCGAGGTG GAGGTTAGGC GGGTGAAGTT TGCCAGAGAT TCTGGCTCTG 
     1921 ATGGTCACAG TGTTAGTTCC CGCGATTCTG CAGCTCCGTC TCCCATCCCT GGTGCTGACA 
     1981 ACCTCTCTGC TGATCCTGTT GTGTCACCAC CCACATCAAT ACCTTTCAAA TCAGGGGAGC 
     2041 CAGCTCTTCG TACCAAATCT AACTCCCTCA GTGAACAACT TGCAATAAAT ACAAGTCCCG 
     2101 ATGCAGTCAA AGCCAAGTTG ATCTCTCGGA TGGCTAAAAT GGGCCAGCCC ATGCTGCCCA 
     2161 TCCTTCCACC ACAGCTGGAT TCCAATGATT CAGAAATCGA AGATGTGAAC ACTCTGCAAG 
     2221 GAGGTGGGCA GCCTGTGGTG ACTCCGTCCG TCCAGCCCTC TCTTCATCCG GCCCATCCAG 
     2281 CGTTACCACA GATGACCTCA CAGGCACCTC AGCCATCTGT TACTGGGCTC CAGGCACCTT 
     2341 CTGCTGCCTT AATGCAAGTG TCATCTCTCG ATTCCCACTC AGCTGTATCT GGAAATGCCC 
     2401 AATCCTTTCA GCCCTATGCA GGTATGCAAG CCTACGCTTA TCCCCAGGCA TCTGCCGTCA 
     2461 CCTCCCAGCT GCAGCCCGTT CGGCCTTTGT ACCCAGCACC GCTCTCTCAG CCTCCCCATT 
     2521 TCCAAGGATC AGGTGATATG GCTTCATTTC TCATGACTGA AGCCCGGCAA CATAACACTG 
     2581 AAATTCGAAT GGCAGTCAGC AAAGTGGCTG ATAAAATGGA TCATCTCATG ACTAAGGTTG 
     2641 AAGAGTTACA GAAACATAGT GCTGGCAATT CCATGCTTAT TCCTAGCATG TCAGTTACAA 
     2701 TGGAAACAAG CATGATTATG AGCAACATCC AGCGAATCAT TCAGGAAAAT GAAAGATTGA 
     2761 AGCAAGAGAT CCTTGAAAAG AGCAATCGGA TAGAAGAACA GAATGACAAG ATTAGTGAAC 
     2821 TAATTGAACG AAATCAGAGG TATGTTGAGC AGAGTAACCT GATGATGGAG AAGAGGAACA 
     2881 ACTCACTTCA GACAGCCACA GAAAACACAC AGGCAAGAGT ATTGCATGCT GAACAAGAGA 
     2941 AGGCCAAGGT GACAGAGGAG TTAGCAGCGG CCACTGCACA GGTCTCTCAT CTGCAGCTGA 
     3001 AAATGACTGC TCACCAAAAA AAGGAAACAG AGCTGCAGAT GCAGCTGACA GAAAGCCTGA 
     3061 AGGAGACAGA TCTTCTCAGG GGCCAGCTCA CCAAAGTGCA GGCAAAGCTC TCAGAGCTCC 
     3121 AAGAAACCTC TGAGCAAGCA CAGTCCAAAT TCAAAAGTGA AAAGCAGAAC CGGAAACAAC 
     3181 TGGAACTCAA GGTGACATCC CTGGAGGAGG AACTGACTGA CCTTCGAGTT GAGAAGGAGT 
     3241 CCTTGGAAAA GAACCTCTCA GAAAGGAAAA AGAAGTCAGC TCAAGAGCGT TCTCAGGCCG 
     3301 AGGAGGAGAT AGATGAAATT CGCAAGTCAT ACCAGGAGGA ATTGGACAAA CTTCGACAGC 
     3361 TCTTGAAAAA GACTCGAGTG TCCACAGACC AAGCAGCTGC AGAGCAGCTG TCTTTAGTAC 
     3421 AGGCTGAGCT ACAGACCCAG TGGGAAGCAA AATGTGAACA TTTGTTGGCC TCCGCCAAGG 
     3481 ATGAGCACCT GCAGCAGTAC CAGGAGGTGT GCGCACAGAG AGATGCCTAC CAGCAGAAGC 
     3541 TGGTACAACT TCAGGAAAAG TGTTTAGCCC TCCAGGCCCA AATCACAGCT CTCACCAAGC 
     3601 AAAATGAACA GCACATCAAG GAACTAGAGA AGAACAAGTC CCAGATGTCT GGGGTTGAAG 
     3661 CTGCTGCATC TGACCCCTCA GAGAAGGTCA AGAAGATCAT GAACCAGGTG TTCCAGTCCT 
     3721 TACGGAGAGA GTTTGAGCTG GAGGAATCTT ACAATGGCAG GACCATTCTG GGAACCATCA 
     3781 TGAATACGAT CAAGATGGTG ACTCTTCAGC TGTTAAACCA ACAGGAGCAA GAGAAGGAAG 
     3841 AGAGCAGCAG TGAAGAAGAA GAAGAAAAAG CAGAAGAGCG GCCACGAAGA CCTTCCCAGG 
     3901 AGCAGTCAGC CTCAGCCAGT TCTGGGCAGC CTCAAGCACC CCTGAATAGG GAGAGGCCAG 
     3961 AGTCCCCCAT GGTGCCCTCA GAGCAGGTGG TCGAGGAAGC TGTCCCGTTG CCTCCTCAGG 
     4021 CCCTCACCAC TTCCCAGGAT GGACACAGAA GGAAAGGGGA CTCAGAAGCT GAGGCACTCT 
     4081 CAGAGATAAA AGATGGTTCC CTTCCACCCG AACTGTCTTG CATCCCATCC CACAGAGTTC 
     4141 TAGGGCCCCC GACTTCAATT CCACCTGAGC CCCTAGGCCC TGTATCCATG GACTCTGAGT 
     4201 GTGAGGAGTC ACTTGCTGCC AGCCCAATGG CAGCTAAGCC CGACAACCCA TCAGGAAAGG 
     4261 TCTGTGTCAG GGAAGTAGCA CCAGATGGCC CACTACAAGA AAGCTCCACA AGACTGTCCC 
     4321 TGACTTCAGA CCCCGAGGAG GGGGACCCAC TGGCCTTAGG GCCTGAAAGC CCAGGAGAGC 
     4381 CTCAGCCTCC ACAGCTCAAG AAAGATGATG TCACTAGCTC CACCGGTCCC CACAAGGAGC 
     4441 TGTCAAGCAC AGAGGCAGGT TCCACAGTTG CAGGAGCAGC CCTCAGACCC AGCCATCATT 
     4501 CCCAGCGTTC CAGTCTCTCT GGGGATGAAG AGGATGAACT GTTTAAAGGG GCAACTCTGA 
     4561 AAGCTCTGAG GCCCAAAGCA CAGCCTGAGG AGGAGGATGA AGACGAGGTG AGCATGAAGG 
     4621 GACGCCCGCC CCCAACGCCC CTTTTTGGAG ATGATGATGA TGACGATGAC ATTGACTGGC 
     4681 TGGGAACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     4741 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     4801 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4861 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     4921 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     4981 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     5041 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     5101 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     5161 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     5221 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     5281 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     5341 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     5401 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     5461 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     5521 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     5581 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     5641 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     5701 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     5761 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     5821 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     5881 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     5941 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     6001 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     6061 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     6121 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     6181 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     6241 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     6301 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     6361 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     6421 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     6481 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     6541 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     6601 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     6661 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     6721 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     6781 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     6841 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     6901 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     6961 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     7021 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     7081 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     7141 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     7201 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     7261 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     7321 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     7381 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     7441 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     7501 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     7561 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     7621 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     7681 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     7741 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     7801 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7861 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7921 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     7981 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     8041 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     8101 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     8161 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     8221 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     8281 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     8341 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     8401 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     8461 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     8521 AAATTTAACG CGAATTTT
//