LOCUS       RC215796                7737 bp    DNA     circular  SYN 
DEFINITION  GAA (Myc-DDK-tagged)-Human glucosidase, alpha, acid (GAA), transcript variant 1
ACCESSION   NM_000152
VERSION     NM_000152.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3894..3985 
                     /label="Myc-DDK"  
     misc_feature    4015..4600 
                     /label="PolyA signal"  
     misc_feature    4072..4091 
                     /label="Restriction Enzyme"  
     misc_feature    4876..5548 
                     /label="ColE1"  
     misc_feature    5978..6772 
                     /label="Kan/Neo"  
     misc_feature    6807..7109 
                     /label="SV40 ori"  
     misc_feature    7195..7672 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3876..3882 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3876 
                     /label="GAA"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGAGTGAGG CACCCGCCCT GCTCCCACCG GCTCCTGGCC GTCTGCGCCC 
     1081 TCGTGTCCTT GGCAACCGCT GCACTCCTGG GGCACATCCT ACTCCATGAT TTCCTGCTGG 
     1141 TTCCCCGAGA GCTGAGTGGC TCCTCCCCAG TCCTGGAGGA GACTCACCCA GCTCACCAGC 
     1201 AGGGAGCCAG CAGACCAGGG CCCCGGGATG CCCAGGCACA CCCCGGCCGT CCCAGAGCAG 
     1261 TGCCCACACA GTGCGACGTC CCCCCCAACA GCCGCTTCGA TTGCGCCCCT GACAAGGCCA 
     1321 TCACCCAGGA ACAGTGCGAG GCCCGCGGCT GTTGCTACAT CCCTGCAAAG CAGGGGCTGC 
     1381 AGGGAGCCCA GATGGGGCAG CCCTGGTGCT TCTTCCCACC CAGCTACCCC AGCTACAAGC 
     1441 TGGAGAACCT GAGCTCCTCT GAAATGGGCT ACACGGCCAC CCTGACCCGT ACCACCCCCA 
     1501 CCTTCTTCCC CAAGGACATC CTGACCCTGC GGCTGGACGT GATGATGGAG ACTGAGAACC 
     1561 GCCTCCACTT CACGATCAAA GATCCAGCTA ACAGGCGCTA CGAGGTGCCC TTGGAGACCC 
     1621 CGCATGTCCA CAGCCGGGCA CCGTCCCCAC TCTACAGCGT GGAGTTCTCC GAGGAGCCCT 
     1681 TCGGGGTGAT CGTGCGCCGG CAGCTGGACG GCCGCGTGCT GCTGAACACG ACGGTGGCGC 
     1741 CCCTGTTCTT TGCGGACCAG TTCCTTCAGC TGTCCACCTC GCTGCCCTCG CAGTATATCA 
     1801 CAGGCCTCGC CGAGCACCTC AGTCCCCTGA TGCTCAGCAC CAGCTGGACC AGGATCACCC 
     1861 TGTGGAACCG GGACCTTGCG CCCACGCCCG GTGCGAACCT CTACGGGTCT CACCCTTTCT 
     1921 ACCTGGCGCT GGAGGACGGC GGGTCGGCAC ACGGGGTGTT CCTGCTAAAC AGCAATGCCA 
     1981 TGGATGTGGT CCTGCAGCCG AGCCCTGCCC TTAGCTGGAG GTCGACAGGT GGGATCCTGG 
     2041 ATGTCTACAT CTTCCTGGGC CCAGAGCCCA AGAGCGTGGT GCAGCAGTAC CTGGACGTTG 
     2101 TGGGATACCC GTTCATGCCG CCATACTGGG GCCTGGGCTT CCACCTGTGC CGCTGGGGCT 
     2161 ACTCCTCCAC CGCTATCACC CGCCAGGTGG TGGAGAACAT GACCAGGGCC CACTTCCCCC 
     2221 TGGACGTCCA GTGGAACGAC CTGGACTACA TGGACTCCCG GAGGGACTTC ACGTTCAACA 
     2281 AGGATGGCTT CCGGGACTTC CCGGCCATGG TGCAGGAGCT GCACCAGGGC GGCCGGCGCT 
     2341 ACATGATGAT CGTGGATCCT GCCATCAGCA GCTCGGGCCC TGCCGGGAGC TACAGGCCCT 
     2401 ACGACGAGGG TCTGCGGAGG GGGGTTTTCA TCACCAACGA GACCGGCCAG CCGCTGATTG 
     2461 GGAAGGTATG GCCCGGGTCC ACTGCCTTCC CCGACTTCAC CAACCCCACA GCCCTGGCCT 
     2521 GGTGGGAGGA CATGGTGGCT GAGTTCCATG ACCAGGTGCC CTTCGACGGC ATGTGGATTG 
     2581 ACATGAACGA GCCTTCCAAC TTCATCAGGG GCTCTGAGGA CGGCTGCCCC AACAATGAGC 
     2641 TGGAGAACCC ACCCTACGTG CCTGGGGTGG TTGGGGGGAC CCTCCAGGCG GCCACCATCT 
     2701 GTGCCTCCAG CCACCAGTTT CTCTCCACAC ACTACAACCT GCACAACCTC TACGGCCTGA 
     2761 CCGAAGCCAT CGCCTCCCAC AGGGCGCTGG TGAAGGCTCG GGGGACACGC CCATTTGTGA 
     2821 TCTCCCGCTC GACCTTTGCT GGCCACGGCC GATACGCCGG CCACTGGACG GGGGACGTGT 
     2881 GGAGCTCCTG GGAGCAGCTC GCCTCCTCCG TGCCAGAAAT CCTGCAGTTT AACCTGCTGG 
     2941 GGGTGCCTCT GGTCGGGGCC GACGTCTGCG GCTTCCTGGG CAACACCTCA GAGGAGCTGT 
     3001 GTGTGCGCTG GACCCAGCTG GGGGCCTTCT ACCCCTTCAT GCGGAACCAC AACAGCCTGC 
     3061 TCAGTCTGCC CCAGGAGCCG TACAGCTTCA GCGAGCCGGC CCAGCAGGCC ATGAGGAAGG 
     3121 CCCTCACCCT GCGCTACGCA CTCCTCCCCC ACCTCTACAC ACTGTTCCAC CAGGCCCACG 
     3181 TCGCGGGGGA GACCGTGGCC CGGCCCCTCT TCCTGGAGTT CCCCAAGGAC TCTAGCACCT 
     3241 GGACTGTGGA CCACCAGCTC CTGTGGGGGG AGGCCCTGCT CATCACCCCA GTGCTCCAGG 
     3301 CCGGGAAGGC CGAAGTGACT GGCTACTTCC CCTTGGGCAC ATGGTACGAC CTGCAGACGG 
     3361 TGCCAGTAGA GGCCCTTGGC AGCCTCCCAC CCCCACCTGC AGCTCCCCGT GAGCCAGCCA 
     3421 TCCACAGCGA GGGGCAGTGG GTGACGCTGC CGGCCCCCCT GGACACCATC AACGTCCACC 
     3481 TCCGGGCTGG GTACATCATC CCCCTGCAGG GCCCTGGCCT CACAACCACA GAGTCCCGCC 
     3541 AGCAGCCCAT GGCCCTGGCT GTGGCCCTGA CCAAGGGTGG GGAGGCCCGA GGGGAGCTGT 
     3601 TCTGGGACGA TGGAGAGAGC CTGGAAGTGC TGGAGCGAGG GGCCTACACA CAGGTCATCT 
     3661 TCCTGGCCAG GAATAACACG ATCGTGAATG AGCTGGTACG TGTGACCAGT GAGGGAGCTG 
     3721 GCCTGCAGCT GCAGAAGGTG ACTGTCCTGG GCGTGGCCAC GGCGCCCCAG CAGGTCCTCT 
     3781 CCAACGGTGT CCCTGTCTCC AACTTCACCT ACAGCCCCGA CACCAAGGTC CTGGACATCT 
     3841 GTGTCTCGCT GTTGATGGGA GAGCAGTTTC TCGTCAGCTG GTGTACGCGT ACGCGGCCGC 
     3901 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     3961 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4021 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4081 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4141 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4201 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4261 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4321 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     4381 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     4441 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     4501 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     4561 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     4621 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     4681 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     4741 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     4801 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     4861 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     4921 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     4981 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     5041 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     5101 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     5161 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     5221 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     5281 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     5341 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     5401 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     5461 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     5521 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     5581 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     5641 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     5701 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     5761 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     5821 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     5881 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     5941 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     6001 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     6061 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     6121 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     6181 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     6241 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     6301 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     6361 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     6421 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     6481 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     6541 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     6601 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     6661 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     6721 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     6781 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     6841 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     6901 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     6961 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7021 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     7081 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     7141 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     7201 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     7261 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     7321 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     7381 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     7441 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     7501 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     7561 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     7621 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     7681 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//