LOCUS       RC215795                8364 bp    DNA     circular  SYN 
DEFINITION  BMP2K (Myc-DDK-tagged)-Human BMP2 inducible kinase (BMP2K), transcript variant 1
ACCESSION   NM_198892
VERSION     NM_198892.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4521..4612 
                     /label="Myc-DDK"  
     misc_feature    4642..5227 
                     /label="PolyA signal"  
     misc_feature    4699..4718 
                     /label="Restriction Enzyme"  
     misc_feature    5503..6175 
                     /label="ColE1"  
     misc_feature    6605..7399 
                     /label="Kan/Neo"  
     misc_feature    7434..7736 
                     /label="SV40 ori"  
     misc_feature    7822..8299 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4503..4509 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4503 
                     /label="BMP2K"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGAAGTTC TCTCGGATGC CCAAGTCGGA GGGCGGCAGC GGCGGCGGAG 
     1081 CGGCGGGTGG CGGGGCTGGC GGGGCCGGGG CCGGGGCCGG CTGCGGCTCC GGCGGCTCGT 
     1141 CCGTGGGGGT CCGGGTGTTC GCGGTCGGCC GCCACCAGGT CACCCTGGAA GAGTCGCTGG 
     1201 CCGAAGGTGG ATTCTCCACA GTTTTCCTCG TGCGTACTCA CGGTGGAATC CGATGTGCAT 
     1261 TGAAGCGAAT GTATGTCAAT AACATGCCAG ACCTCAATGT TTGTAAAAGG GAAATTACAA 
     1321 TTATGAAAGA GCTATCTGGT CACAAAAATA TTGTGGGCTA TTTGGACTGT GCTGTTAATT 
     1381 CAATTAGTGA TAATGTATGG GAAGTCCTTA TCTTAATGGA ATATTGTCGA GCTGGACAGG 
     1441 TAGTGAATCA AATGAATAAG AAGCTACAGA CGGGTTTTAC AGAACCAGAA GTGTTACAGA 
     1501 TATTCTGTGA TACCTGTGAA GCTGTTGCAA GGTTGCATCA GTGTAAGACT CCAATAATTC 
     1561 ACCGGGATCT GAAGGTAGAA AATATTTTGT TGAATGATGG TGGGAACTAT GTACTTTGTG 
     1621 ACTTTGGCAG TGCCACTAAT AAATTTCTTA ATCCTCAAAA AGATGGAGTT AATGTAGTAG 
     1681 AAGAAGAAAT TAAAAAGTAT ACAACTCTGT CATACAGAGC CCCTGAAATG ATCAACCTTT 
     1741 ATGGAGGGAA ACCCATCACC ACCAAGGCTG ATATCTGGGC ACTGGGATGT CTACTCTATA 
     1801 AACTTTGTTT CTTCACTCTT CCTTTTGGTG AGAGTCAGGT TGCTATCTGT GATGGCAACT 
     1861 TCACCATCCC AGACAATTCT CGTTACTCCC GTAACATACA TTGCTTAATA AGGTTCATGC 
     1921 TTGAACCAGA TCCGGAACAT AGACCTGATA TATTTCAAGT GTCATATTTT GCATTTAAAT 
     1981 TTGCCAAAAA GGATTGTCCA GTCTCCAACA TCAATAATTC TTCTATTCCT TCAGCTCTTC 
     2041 CTGAACCGAT GACTGCTAGT GAAGCAGCTG CTAGGAAAAG CCAAATAAAA GCCAGAATAA 
     2101 CAGATACCAT TGGACCAACA GAAACCTCAA TTGCACCAAG ACAAAGACCA AAGGCCAACT 
     2161 CTGCTACTAC TGCCACTCCC AGTGTGCTGA CCATTCAAAG TTCAGCAACA CCTGTTAAAG 
     2221 TCCTTGCTCC TGGTGAATTC GGTAACCATA GACCAAAAGG GGCACTAAGA CCTGGAAATG 
     2281 GCCCTGAAAT TTTATTGGGT CAGGGACCTC CTCAGCAGCC GCCACAGCAG CATAGAGTAC 
     2341 TCCAGCAACT ACAGCAGGGA GATTGGAGAT TACAGCAACT CCATTTACAG CATCGTCATC 
     2401 CTCACCAGCA GCAGCAGCAG CAGCAGCAGC AACAGCAACA GCAGCAGCAG CAACAGCAAC 
     2461 AGCAGCAGCA GCAGCAGCAG CAGCAGCACC ACCACCACCA CCACCACCAC CTACTTCAAG 
     2521 ATGCTTATAT GCAGCAGTAT CAACATGCAA CACAGCAGCA ACAGATGCTT CAACAACAAT 
     2581 TTTTAATGCA TTCGGTATAT CAACCACAAC CTTCTGCATC ACAGTATCCT ACAATGATGC 
     2641 CGCAGTATCA GCAGGCTTTC TTTCAACAGC AGATGCTAGC TCAACATCAG CCGTCTCAAC 
     2701 AACAGGCATC ACCTGAATAT CTTACCTCCC CTCAAGAGTT CTCACCAGCC TTAGTTTCCT 
     2761 ACACTTCATC ACTTCCAGCT CAGGTTGGAA CCATAATGGA CTCCTCCTAT AGTGCCAATA 
     2821 GGTCAGTTGC TGATAAAGAG GCCATTGCAA ATTTCACAAA TCAGAAGAAC ATCAGCAATC 
     2881 CACCTGATAT GTCAGGGTGG AATCCTTTTG GAGAGGATAA TTTCTCTAAG TTAACAGAAG 
     2941 AGGAACTATT GGACAGAGAA TTTGACCTTC TAAGATCAAA TAGGCTCGAG GAGAGAGCAT 
     3001 CCTCAGATAA GAATGTAGAC TCACTTTCTG CTCCACATAA CCATCCTCCA GAAGATCCTT 
     3061 TTGGTTCTGT TCCTTTCATT TCTCATTCAG GTTCTCCTGA AAAGAAAGCT GAACATTCAT 
     3121 CTATAAATCA AGAAAATGGC ACTGCAAACC CTATCAAGAA CGGTAAAACA AGTCCAGCAT 
     3181 CTAAAGATCA GCGGACTGGA AAGAAAACCT CAGTACAGGG TCAAGTGCAA AAGGGGAATG 
     3241 ATGAATCTGA AAGTGATTTT GAATCAGATC CCCCTTCTCC TAAGAGCAGT GAAGAGGAAG 
     3301 AGCAAGATGA TGAAGAAGTT CTTCAGGGGG AACAAGGAGA TTTTAATGAT GATGATACTG 
     3361 AACCAGAAAA TCTGGGTCAT AGGCCTCTCC TCATGGATTC TGAAGATGAG GAAGAAGAGG 
     3421 AGAAACATAG CTCTGATTCT GATTATGAGC AGGCTAAAGC AAAGTACAGT GACATGAGCT 
     3481 CTGTCTACAG AGACAGATCT GGCAGTGGAC CAACCCAAGA TCTTAATACA ATACTCCTCA 
     3541 CCTCAGCCCA ATTATCCTCT GATGTTGCAG TGGAGACTCC CAAACAGGAG TTTGATGTAT 
     3601 TTGGCGCTGT CCCCTTCTTT GCAGTGCGTG CTCAACAGCC CCAGCAAGAA AAGAATGAAA 
     3661 AGAACCTCCC TCAACACAGG TTTCCTGCTG CAGGACTGGA GCAGGAGGAA TTTGATGTAT 
     3721 TCACAAAGGC GCCTTTTAGC AAGAAGGTGA ATGTACAAGA ATGCCATGCA GTGGGGCCTG 
     3781 AGGCACATAC TATCCCTGGT TATCCCAAAA GTGTAGATGT ATTTGGCTCC ACTCCATTTC 
     3841 AGCCCTTCCT CACATCAACA AGTAAAAGTG AAAGCAATGA GGACCTTTTT GGGCTTGTGC 
     3901 CCTTTGATGA AATAACGGGG AGCCAGCAGC AAAAAGTCAA ACAGCGCAGC TTACAGAAAC 
     3961 TGTCCTCTCG CCAAAGGCGC ACAAAGCAGG ATATGTCCAA AAGTAATGGG AAGCGGCATC 
     4021 ATGGCACGCC AACTAGCACA AAGAAGACTT TGAAGCCTAC CTATCGCACT CCAGAGAGGG 
     4081 CTCGCAGGCA CAAAAAAGTG GGCCGCCGAG ACTCTCAAAG TAGCAATGAA TTTTTAACCA 
     4141 TCTCAGACTC CAAGGAGAAC ATTAGTGTTG CACTGACTGA TGGGAAAGAT AGGGGGAATG 
     4201 TCTTACAACC TGAGGAGAGC CTGTTGGACC CCTTCGGTGC CAAGCCCTTC CATTCTCCAG 
     4261 ACCTGTCATG GCACCCTCCA CATCAGGGCC TGAGCGACAT CCGTGCTGAT CACAATACTG 
     4321 TCCTGCCAGG GCGGCCAAGA CAAAATTCAC TACATGGGTC ATTCCATAGT GCAGATGTAT 
     4381 TGAAAATGGA TGATTTTGGT GCCGTGCCCT TTACAGAACT TGTGGTGCAA AGCATCACTC 
     4441 CACATCAGTC CCAACAGTCC CAACCAGTCG AATTAGACCC ATTTGGTGCT GCTCCATTTC 
     4501 CTTCTAAACA GACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     4561 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4621 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4681 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4741 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4801 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4861 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4921 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4981 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     5041 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     5101 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     5161 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     5221 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     5281 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     5341 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     5401 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     5461 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     5521 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     5581 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     5641 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     5701 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     5761 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     5821 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     5881 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     5941 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     6001 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     6061 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     6121 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     6181 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     6241 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6301 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6361 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6421 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     6481 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     6541 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6601 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     6661 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6721 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6781 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     6841 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     6901 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     6961 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     7021 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     7081 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     7141 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     7201 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     7261 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7321 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7381 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7441 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     7501 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     7561 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7621 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7681 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7741 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7801 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     7861 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     7921 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     7981 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     8041 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     8101 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     8161 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     8221 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     8281 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     8341 TAACAAAAAT TTAACGCGAA TTTT
//