LOCUS       RC215776                8676 bp    DNA     circular  SYN 
DEFINITION  KDM2B (Myc-DDK-tagged)-Human lysine (K)-specific demethylase 2B (KDM2B), transcript variant 2
ACCESSION   NM_001005366
VERSION     NM_001005366.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4833..4924 
                     /label="Myc-DDK"  
     misc_feature    4954..5539 
                     /label="PolyA signal"  
     misc_feature    5011..5030 
                     /label="Restriction Enzyme"  
     misc_feature    5815..6487 
                     /label="ColE1"  
     misc_feature    6917..7711 
                     /label="Kan/Neo"  
     misc_feature    7746..8048 
                     /label="SV40 ori"  
     misc_feature    8134..8611 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4815..4821 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4815 
                     /label="KDM2B"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGGCAGAG AAAGACTCTG GAAGAAGATT GCGCCCGATT GACCGCCAGC 
     1081 GATACGACGA GAACGAGGAC TTGTCGGACG TGGAGGAGAT CGTCAGCGTC CGCGGCTTCA 
     1141 GCCTGGAGGA GAAGCTTCGC AGCCAGCTGT ACCAGGGGGA CTTCGTGCAC GCCATGGAGG 
     1201 GCAAAGATTT CAACTATGAG TACGTACAGA GAGAAGCTCT CAGGGTTCCC CTGATATTTC 
     1261 GAGAAAAGGA TGGACTGGGA ATTAAGATGC CTGACCCTGA TTTCACAGTC CGAGACGTCA 
     1321 AACTCCTAGT GGGGAGCCGG CGGCTTGTGG ACGTGATGGA TGTGAACACC CAGAAGGGCA 
     1381 CGGAGATGAG CATGTCCCAG TTTGTGCGTT ACTACGAGAC GCCCGAGGCC CAGCGGGACA 
     1441 AGCTGTACAA CGTCATCAGC CTAGAGTTCA GCCACACCAA GCTGGAGCAC TTGGTCAAGC 
     1501 GTCCGACTGT GGTAGACCTG GTGGACTGGG TGGACAACAT GTGGCCCCAG CATCTGAAGG 
     1561 AGAAGCAGAC AGAAGCCACG AACGCCATTG CAGAGATGAA GTACCCGAAA GTGAAAAAGT 
     1621 ACTGTCTGAT GAGCGTGAAA GGTTGTTTCA CCGACTTCCA CATCGACTTT GGAGGCACTT 
     1681 CCGTTTGGTA CCATGTTTTC CGGGGTGGGA AGATTTTTTG GCTGATTCCT CCAACGCTGC 
     1741 ACAATTTGGC GCTGTACGAG GAGTGGGTGC TGTCAGGCAA ACAGAGTGAC ATCTTTCTGG 
     1801 GAGACCGTGT GGAACGATGC CAAAGAATTG AGCTGAAGCA GGGCTACACA TTTTTCATCC 
     1861 CTTCCGGTTG GATCCATGCC GTCTACACCC CTGTAGACTC TTTGGTGTTC GGCGGAAACA 
     1921 TCCTGCACAG CTTTAACGTG CCCATGCAGC TGCGGATCTA CGAGATCGAG GACAGGACGC 
     1981 GGGTGCAGCC CAAATTCCGT TACCCCTTCT ACTATGAGAT GTGCTGGTAT GTCCTGGAGA 
     2041 GATACGTGTA CTGTGTGACC CAGCGCTCCC ACCTCACTCA GGAATACCAG AGGGAGTCGA 
     2101 TGCTTATTGA TGCCCCGAGG AAGCCCAGCA TAGACGGCTT CTCTTCGGAT TCCTGGCTGG 
     2161 AGATGGAGGA GGAGGCCTGT GATCAGCAGC CTCAGGAGGA GGAGGAGAAG GACGAGGAGG 
     2221 GCGAGGGCAG GGACAGGGCA CCCAAACCGC CCACCGATGG CTCCACTTCA CCCACCAGCA 
     2281 CGCCCTCTGA GGACCAGGAG GCCCTCGGGA AGAAGCCCAA AGCACCTGCC CTGCGATTCC 
     2341 TCAAAAGGAC TTTGTCTAAT GAGTCGGAGG AAAGTGTGAA GTCCACCACA TTGGCCGTAG 
     2401 ACTACCCCAA GACCCCCACC GGCTCTCCCG CCACGGAGGT CTCTGCCAAA TGGACCCATC 
     2461 TCACTGAGTT TGAACTGAAG GGCCTGAAAG CTCTGGTGGA GAAACTGGAA TCCCTCCCGG 
     2521 AGAACAAGAA GTGTGTCCCC GAGGGCATCG AGGACCCCCA GGCACTCCTG GAGGGTGTGA 
     2581 AGAACGTCCT GAAGGAGCAC GCAGATGATG ACCCTAGTCT GGCCATCACT GGGGTCCCTG 
     2641 TGGTGACTTG GCCAAAGAAG ACTCCAAAGA ACCGGGCTGT GGGTCGGCCC AAGGGGAAGC 
     2701 TGGGCCCGGC CTCCGCGGTG AAGTTGGCCG CCAACCGGAC AACGGCAGGA GCTCGGCGGC 
     2761 GCCGGACGCG ATGCCGCAAG TGCGAGGCCT GCCTGCGGAC CGAGTGCGGA GAGTGCCACT 
     2821 TCTGCAAGGA CATGAAGAAG TTCGGGGGCC CCGGGCGCAT GAAGCAGAGC TGCATCATGC 
     2881 GGCAGTGCAT CGCGCCAGTG CTGCCCCACA CCGCCGTGTG CCTTGTGTGT GGCGAGGCGG 
     2941 GGAAGGAAGA CACGGTGGAA GAGGAGGAAG GCAAGTTTAA CCTCATGCTC ATGGAGTGCT 
     3001 CCATCTGCAA TGAAATCATC CACCCTGGAT GCCTTAAGAT TAAGGAGTCA GAGGGTGTGG 
     3061 TCAACGACGA GCTTCCAAAC TGCTGGGAGT GTCCGAAGTG TAACCACGCC GGCAAGACCG 
     3121 GGAAACAAAA GCGTGGCCCT GGCTTTAAGT ACGCCTCCAA CCTGCCCGGC TCCCTGCTCA 
     3181 AGGAGCAGAA GATGAACCGG GACAACAAGG AAGGGCAGGA ACCTGCCAAG CGGAGGAGTG 
     3241 AGTGTGAGGA GGCGCCCCGG CGCAGGTCGG ATGAGCACTC GAAGAAGGTG CCGCCGGACG 
     3301 GCCTTCTGCG CAGAAAGTCT GACGACGTGC ACCTGAGGAA GAAGCGGAAA TACGAGAAGC 
     3361 CCCAGGAGCT GAGTGGACGC AAGCGGCTCA AACCTGGCAA AGAAGATAAG CTTTTCAGGA 
     3421 AAAAGCGGCG GTCCTGGAAG AACGCCGAGG ACCGCATGGC GCTGGCCAAC AAGCCCCTCC 
     3481 GGCGCTTCAA GCAGGAACCC GAGGACGAAC TGCCCGAGGC GCCCCCCAAG ACCAGGGAGA 
     3541 GCGACCACTC CCGCTCCAGC TCCCCCACCG CGGGACCCAG CACCGAAGGG GCCGAGGGCC 
     3601 CGGAGGAGAA GAAGAAGGTG AAGATGCGCC GGAAGCGGCG GCTTCCCAAC AAGGAGCTGA 
     3661 GCAGGGAGCT GAGCAAGGAG CTCAACCACG AGATCCAGAG GACGGAGAAC AGCCTGGCCA 
     3721 ACGAGAACCA GCAGCCCATC AAGTCGGAGC CTGAGAGCGA GGGCGAGGAG CCCAAGCGGC 
     3781 CCCCGGGCAT CTGCGAGCGT CCCCACCGCT TCAGCAAGGG GCTCAACGGC ACCCCCCGGG 
     3841 AGCTGCGGCA CCAGCTGGGG CCCAGCCTGC GCAGCCCGCC CCGTGTCATC TCCCGGCCCC 
     3901 CACCCTCCGT GTCCCCGCCC AAGTGTATCC AGATGGAGCG CCATGTGATC CGGCCACCCC 
     3961 CCATCAGCCC CCCGCCTGAC TCGCTACCCC TGGACGATGG GGCAGCCCAC GTCATGCACA 
     4021 GGGAGGTGTG GATGGCCGTC TTCAGCTACC TCAGCCACCA AGACCTGTGT GTGTGCATGC 
     4081 GGGTCTGCAG GACCTGGAAC CGCTGGTGCT GCGATAAGCG GTTGTGGACC CGCATTGACC 
     4141 TGAACCACTG CAAGTCTATC ACACCCCTGA TGCTGAGTGG CATCATCCGG CGACAGCCCG 
     4201 TCTCCCTCGA CCTCAGCTGG ACCAATATCT CCAAGAAGCA GCTGAGCTGG CTCATCAACC 
     4261 GGCTGCCTGG GCTCCGGGAC TTGGTGCTGT CAGGCTGCTC ATGGATCGCG GTCTCGGCCC 
     4321 TTTGCAGCTC CAGTTGTCCG CTGCTCCGGA CCCTGGATGT CCAGTGGGTG GAGGGACTAA 
     4381 AGGATGCCCA GATGCGGGAT CTCCTGTCCC CGCCCACAGA CAACAGGCCA GGTCAGATGG 
     4441 ACAATCGGAG CAAGCTCCGG AACATCGTGG AGCTGCGCCT GGCAGGCCTG GACATCACAG 
     4501 ATGCCTCCCT GCGGCTCATC ATCCGCCACA TGCCCCTGCT CTCCAAGCTC CACCTCAGTT 
     4561 ACTGTAACCA CGTCACCGAC CAGTCTATCA ACCTGCTCAC TGCTGTTGGC ACCACCACCC 
     4621 GAGACTCCTT AACCGAGATC AACCTGTCTG ACTGCAATAA GGTCACTGAT CAGTGCCTGT 
     4681 CCTTCTTCAA ACGCTGTGGA AACATCTGTC ATATTGACCT GAGGTACTGC AAGCAAGTCA 
     4741 CCAAGGAAGG CTGTGAGCAG TTCATAGCCG AGATGTCTTC TTTCCAAGGG AGAAGCTGTT 
     4801 CAACCACCCG TTTGGGGGAT GAGACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4861 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4921 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4981 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     5041 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     5101 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     5161 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     5221 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     5281 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     5341 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     5401 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     5461 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5521 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5581 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     5641 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     5701 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     5761 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5821 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5881 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5941 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     6001 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     6061 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     6121 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     6181 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     6241 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     6301 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     6361 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     6421 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     6481 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     6541 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6601 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     6661 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6721 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6781 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6841 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6901 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6961 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     7021 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     7081 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     7141 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     7201 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     7261 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     7321 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     7381 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     7441 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     7501 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     7561 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7621 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     7681 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7741 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7801 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7861 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7921 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7981 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     8041 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     8101 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     8161 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     8221 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     8281 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     8341 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     8401 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     8461 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     8521 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     8581 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     8641 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//