LOCUS       RC215770                8376 bp    DNA     circular  SYN 
DEFINITION  TET2 (Myc-DDK-tagged)-Human tet oncogene family member 2 (TET2), transcript variant 2
ACCESSION   NM_017628
VERSION     NM_017628.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4533..4624 
                     /label="Myc-DDK"  
     misc_feature    4654..5239 
                     /label="PolyA signal"  
     misc_feature    4711..4730 
                     /label="Restriction Enzyme"  
     misc_feature    5515..6187 
                     /label="ColE1"  
     misc_feature    6617..7411 
                     /label="Kan/Neo"  
     misc_feature    7446..7748 
                     /label="SV40 ori"  
     misc_feature    7834..8311 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4515..4521 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4515 
                     /label="TET2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAACAGGAT AGAACCAACC ATGTTGAGGG CAACAGACTA AGTCCATTCC 
     1081 TGATACCATC ACCTCCCATT TGCCAGACAG AACCTCTGGC TACAAAGCTC CAGAATGGAA 
     1141 GCCCACTGCC TGAGAGAGCT CATCCAGAAG TAAATGGAGA CACCAAGTGG CACTCTTTCA 
     1201 AAAGTTATTA TGGAATACCC TGTATGAAGG GAAGCCAGAA TAGTCGTGTG AGTCCTGACT 
     1261 TTACACAAGA AAGTAGAGGG TATTCCAAGT GTTTGCAAAA TGGAGGAATA AAACGCACAG 
     1321 TTAGTGAACC TTCTCTCTCT GGGCTCCTTC AGATCAAGAA ATTGAAACAA GACCAAAAGG 
     1381 CTAATGGAGA AAGACGTAAC TTCGGGGTAA GCCAAGAAAG AAATCCAGGT GAAAGCAGTC 
     1441 AACCAAATGT CTCCGATTTG AGTGATAAGA AAGAATCTGT GAGTTCTGTA GCCCAAGAAA 
     1501 ATGCAGTTAA AGATTTCACC AGTTTTTCAA CACATAACTG CAGTGGGCCT GAAAATCCAG 
     1561 AGCTTCAGAT TCTGAATGAG CAGGAGGGGA AAAGTGCTAA TTACCATGAC AAGAACATTG 
     1621 TATTACTTAA AAACAAGGCA GTGCTAATGC CTAATGGTGC TACAGTTTCT GCCTCTTCCG 
     1681 TGGAACACAC ACATGGTGAA CTCCTGGAAA AAACACTGTC TCAATATTAT CCAGATTGTG 
     1741 TTTCCATTGC GGTGCAGAAA ACCACATCTC ACATAAATGC CATTAACAGT CAGGCTACTA 
     1801 ATGAGTTGTC CTGTGAGATC ACTCACCCAT CGCATACCTC AGGGCAGATC AATTCCGCAC 
     1861 AGACCTCTAA CTCTGAGCTG CCTCCAAAGC CAGCTGCAGT GGTGAGTGAG GCCTGTGATG 
     1921 CTGATGATGC TGATAATGCC AGTAAACTAG CTGCAATGCT AAATACCTGT TCCTTTCAGA 
     1981 AACCAGAACA ACTACAACAA CAAAAATCAG TTTTTGAGAT ATGCCCATCT CCTGCAGAAA 
     2041 ATAACATCCA GGGAACCACA AAGCTAGCGT CTGGTGAAGA ATTCTGTTCA GGTTCCAGCA 
     2101 GCAATTTGCA AGCTCCTGGT GGCAGCTCTG AACGGTATTT AAAACAAAAT GAAATGAATG 
     2161 GTGCTTACTT CAAGCAAAGC TCAGTGTTCA CTAAGGATTC CTTTTCTGCC ACTACCACAC 
     2221 CACCACCACC ATCACAATTG CTTCTTTCTC CCCCTCCTCC TCTTCCACAG GTTCCTCAGC 
     2281 TTCCTTCAGA AGGAAAAAGC ACTCTGAATG GTGGAGTTTT AGAAGAACAC CACCACTACC 
     2341 CCAACCAAAG TAACACAACA CTTTTAAGGG AAGTGAAAAT AGAGGGTAAA CCTGAGGCAC 
     2401 CACCTTCCCA GAGTCCTAAT CCATCTACAC ATGTATGCAG CCCTTCTCCG ATGCTTTCTG 
     2461 AAAGGCCTCA GAATAATTGT GTGAACAGGA ATGACATACA GACTGCAGGG ACAATGACTG 
     2521 TTCCATTGTG TTCTGAGAAA ACAAGACCAA TGTCAGAACA CCTCAAGCAT AACCCACCAA 
     2581 TTTTTGGTAG CAGTGGAGAG CTACAGGACA ACTGCCAGCA GTTGATGAGA AACAAAGAGC 
     2641 AAGAGATTCT GAAGGGTCGA GACAAGGAGC AAACACGAGA TCTTGTGCCC CCAACACAGC 
     2701 ACTATCTGAA ACCAGGATGG ATTGAATTGA AGGCCCCTCG TTTTCACCAA GCGGAATCCC 
     2761 ATCTAAAACG TAATGAGGCA TCACTGCCAT CAATTCTTCA GTATCAACCC AATCTCTCCA 
     2821 ATCAAATGAC CTCCAAACAA TACACTGGAA ATTCCAACAT GCCTGGGGGG CTCCCAAGGC 
     2881 AAGCTTACAC CCAGAAAACA ACACAGCTGG AGCACAAGTC ACAAATGTAC CAAGTTGAAA 
     2941 TGAATCAAGG GCAGTCCCAA GGTACAGTGG ACCAACATCT CCAGTTCCAA AAACCCTCAC 
     3001 ACCAGGTGCA CTTCTCCAAA ACAGACCATT TACCAAAAGC TCATGTGCAG TCACTGTGTG 
     3061 GCACTAGATT TCATTTTCAA CAAAGAGCAG ATTCCCAAAC TGAAAAACTT ATGTCCCCAG 
     3121 TGTTGAAACA GCACTTGAAT CAACAGGCTT CAGAGACTGA GCCATTTTCA AACTCACACC 
     3181 TTTTGCAACA TAAGCCTCAT AAACAGGCAG CACAAACACA ACCATCCCAG AGTTCACATC 
     3241 TCCCTCAAAA CCAGCAACAG CAGCAAAAAT TACAAATAAA GAATAAAGAG GAAATACTCC 
     3301 AGACTTTTCC TCACCCCCAA AGCAACAATG ATCAGCAAAG AGAAGGATCA TTCTTTGGCC 
     3361 AGACTAAAGT GGAAGAATGT TTTCATGGTG AAAATCAGTA TTCAAAATCA AGCGAGTTCG 
     3421 AGACTCATAA TGTCCAAATG GGACTGGAGG AAGTACAGAA TATAAATCGT AGAAATTCCC 
     3481 CTTATAGTCA GACCATGAAA TCAAGTGCAT GCAAAATACA GGTTTCTTGT TCAAACAATA 
     3541 CACACCTAGT TTCAGAGAAT AAAGAACAGA CTACACATCC TGAACTTTTT GCAGGAAACA 
     3601 AGACCCAAAA CTTGCATCAC ATGCAATATT TTCCAAATAA TGTGATCCCA AAGCAAGATC 
     3661 TTCTTCACAG GTGCTTTCAA GAACAGGAGC AGAAGTCACA ACAAGCTTCA GTTCTACAGG 
     3721 GATATAAAAA TAGAAACCAA GATATGTCTG GTCAACAAGC TGCGCAACTT GCTCAGCAAA 
     3781 GGTACTTGAT ACATAACCAT GCAAATGTTT TTCCTGTGCC TGACCAGGGA GGAAGTCACA 
     3841 CTCAGACCCC TCCCCAGAAG GACACTCAAA AGCATGCTGC TCTAAGGTGG CATCTCTTAC 
     3901 AGAAGCAAGA ACAGCAGCAA ACACAGCAAC CCCAAACTGA GTCTTGCCAT AGTCAGATGC 
     3961 ACAGGCCAAT TAAGGTGGAA CCTGGATGCA AGCCACATGC CTGTATGCAC ACAGCACCAC 
     4021 CAGAAAACAA AACATGGAAA AAGGTAACTA AGCAAGAGAA TCCACCTGCA AGCTGTGATA 
     4081 ATGTGCAGCA AAAGAGCATC ATTGAGACCA TGGAGCAGCA TCTGAAGCAG TTTCACGCCA 
     4141 AGTCGTTATT TGACCATAAG GCTCTTACTC TCAAATCACA GAAGCAAGTA AAAGTTGAAA 
     4201 TGTCAGGGCC AGTCACAGTT TTGACTAGAC AAACCACTGC TGCAGAACTT GATAGCCACA 
     4261 CCCCAGCTTT AGAGCAGCAA ACAACTTCTT CAGAAAAGAC ACCAACCAAA AGAACAGCTG 
     4321 CTTCTGTTCT CAATAATTTT ATAGAGTCAC CTTCCAAATT ACTAGATACT CCTATAAAAA 
     4381 ATTTATTGGA TACACCTGTC AAGACTCAAT ATGATTTCCC ATCTTGCAGA TGTGTAGGTA 
     4441 AGTGCCAGAA ATGTACTGAG ACACATGGCG TTTATCCAGA ATTAGCAAAT TTATCTTCAG 
     4501 ATATGGGATT TTCCTTCTTT TTTACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4561 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4621 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4681 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4741 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4801 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4861 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     4921 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     4981 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     5041 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     5101 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     5161 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5221 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5281 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     5341 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     5401 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     5461 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5521 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5581 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5641 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     5701 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     5761 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     5821 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     5881 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     5941 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     6001 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     6061 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     6121 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     6181 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     6241 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6301 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     6361 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6421 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6481 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6541 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6601 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6661 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     6721 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     6781 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     6841 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     6901 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     6961 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     7021 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     7081 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     7141 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     7201 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     7261 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7321 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     7381 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7441 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7501 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7561 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7621 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7681 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7741 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     7801 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     7861 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     7921 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     7981 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     8041 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     8101 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     8161 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     8221 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     8281 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     8341 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//