LOCUS       RC215762                6579 bp    DNA     circular  SYN 
DEFINITION  INSM2 (Myc-DDK-tagged)-Human insulinoma-associated 2 (INSM2)
ACCESSION   NM_032594
VERSION     NM_032594.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2736..2827 
                     /label="Myc-DDK"  
     misc_feature    2857..3442 
                     /label="PolyA signal"  
     misc_feature    2914..2933 
                     /label="Restriction Enzyme"  
     misc_feature    3718..4390 
                     /label="ColE1"  
     misc_feature    4820..5614 
                     /label="Kan/Neo"  
     misc_feature    5649..5951 
                     /label="SV40 ori"  
     misc_feature    6037..6514 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2718..2724 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2718 
                     /label="INSM2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCAAGGGGA TTCCTGGTGA AGCGAACTAA ACGGACAGGC GGCTTGTACC 
     1081 GAGTTCGCCT TGCGGAGCGT GTCTTCCCTC TGCTGGGGCC CCAGGGGGCG CCGCCCTTCT 
     1141 TGGAGGAGGC TCCCAGCGCC TCCTTGCCCG GCGCGGAGCG GGCGACACCC CCCACCCGAG 
     1201 AGGAACCAGG AAAGGGGTTG ACGGCGGAGG CGGCCCGGGA ACAGTCGGGG TCGCCATGTC 
     1261 GGGCGGCTGG GGTGAGCCCG GGGACGGGCG GGCGGGAAGG CGCGGAGTGG CGGGCGGGTG 
     1321 GCAGGGAAGG TCCCGGGCCC AGCCCCAGCC CCAGCCCCAG TCCAGCGAAG CCGGCAGGCG 
     1381 CAGAGCTGCG TCGGGCGTTC CTGGAGCGCT GCCTCAGCTC GCCCGTCTCC GCCGAGTCTT 
     1441 TCCCCGGGGG CGCCGCCGCC GTGGCCGCTT TCTCCTGCTC CGTGGCGCCA GCAGCCGCAC 
     1501 CGACCCCGGG GGAGCAGTTT CTGCTGCCGC TTCGGGCGCC GTTCCCAGAG CCCGCGCTTC 
     1561 AGCCGGACCC TGCGCCCCTC TCGGCCGCCC TTCAGAGTCT GAAGCGGGCG GCCGGCGGCG 
     1621 AGCGCCGCGG CAAGGCACCC ACGGGCTGCG CGTCTGGACC CGCGGCCGCG GGAATCAAGA 
     1681 AGCCAAAGGC CATGAGGAAG TTGAGCTTTG CCGATGAGGT GACCACATCC CCTGTCCTGG 
     1741 GCCTGAAGAT CAAGGAGGAG GAGCCCGGAG CGCCGTCCCG GGGCTTGGGG GGCAGCCGCA 
     1801 CGCCACTGGG GGAGTTCATC TGCCAGCTGT GCAAGGAGCA GTACGCAGAC CCCTTCGCGC 
     1861 TGGCCCAGCA CCGCTGCTCC CGCATCGTGC GCGTAGAGTA CCGCTGCCCT GAGTGCGACA 
     1921 AGGTGTTCAG CTGTCCTGCG AACCTGGCCT CCCATCGCCG CTGGCATAAG CCGCGTCCTG 
     1981 CGGCTGCAAA CGCCGCCACA GTCTCCTCCG CCGACGGGAA GCCGCCTTCT TCGTCGTCTT 
     2041 CGTCCTCCCG GGACTCCGGG GCCATTGCAT CTTTTCTGGC GGAGGGAAAG GAGAACAGCC 
     2101 GAATAGAGCG GACTGCGGAT CAGCACCCGC AGGCCAGGGA CAGCTCCGGG GCGGATCAGC 
     2161 ACCCGGACAG CGCCCCGAGG CAGGGCCTCC AGGTGGTGAC GCATCCAGAG CCACCGCTGC 
     2221 CTCAGGGCCC CTACACGGAG GGGGTGTTGG GGCGCCGGGT ACCTGTGCCG GGCAGTACCA 
     2281 GTGGTGGCAG GGGATCCGAG ATTTTCGTGT GCCCATATTG CCACAAAAAG TTTCGTCGCC 
     2341 AAGCCTATCT GCGCAAGCAC CTGAGCACTC ACGAGGCGGG CTCGGCCCGT GCGCTAGCGC 
     2401 CGGGCTTTGG CTCCGAACGC GGTGCCCCAC TTGCCTTCGC TTGCCCATTG TGCGGAGCGC 
     2461 ACTTCCCTAC AGCAGATATC AGGGAGAAGC ACCGGCTGTG GCATGCTGTC CGCGAGGAGC 
     2521 TGCTCCTGCC CGCTCTGGCG GGGGCTCCTC CCGAAACGTC GGGCCCTAGC GGGCCATCTG 
     2581 ACGGGAGTGC CCAGCAAATT TTCTCGTGCA AGCACTGCCC GTCCACTTTT TTTAGCTCTC 
     2641 CAGGGCTGAC CCGGCACATC AATAAGTGCC ACCCCTCAGA AAGCCGGCAA GTGCTGCTGC 
     2701 TGCAGATGCC ACTGCGGCCT GGCTGCACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     2761 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     2821 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     2881 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     2941 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     3001 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     3061 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     3121 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     3181 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     3241 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     3301 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     3361 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     3421 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     3481 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     3541 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     3601 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     3661 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     3721 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     3781 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     3841 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     3901 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     3961 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     4021 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     4081 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     4141 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     4201 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     4261 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     4321 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     4381 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     4441 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     4501 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     4561 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     4621 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     4681 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     4741 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     4801 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     4861 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     4921 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     4981 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     5041 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     5101 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     5161 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     5221 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     5281 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     5341 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     5401 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     5461 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     5521 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     5581 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     5641 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     5701 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     5761 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     5821 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     5881 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     5941 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     6001 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     6061 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     6121 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     6181 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     6241 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     6301 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     6361 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     6421 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     6481 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     6541 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//