LOCUS       RC215761                7269 bp    DNA     circular  SYN 
DEFINITION  TLR6 (Myc-DDK-tagged)-Human toll-like receptor 6 (TLR6)
ACCESSION   NM_006068
VERSION     NM_006068.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3426..3517 
                     /label="Myc-DDK"  
     misc_feature    3547..4132 
                     /label="PolyA signal"  
     misc_feature    3604..3623 
                     /label="Restriction Enzyme"  
     misc_feature    4408..5080 
                     /label="ColE1"  
     misc_feature    5510..6304 
                     /label="Kan/Neo"  
     misc_feature    6339..6641 
                     /label="SV40 ori"  
     misc_feature    6727..7204 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3408..3414 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3408 
                     /label="TLR6"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACCAAAGAC AAAGAACCTA TTGTTAAAAG CTTCCATTTT GTTTGCCTTA 
     1081 TGATCATAAT AGTTGGAACC AGAATCCAGT TCTCCGACGG AAATGAATTT GCAGTAGACA 
     1141 AGTCAAAAAG AGGTCTTATT CATGTTCCAA AAGACCTACC GCTGAAAACC AAAGTCTTAG 
     1201 ATATGTCTCA GAACTACATC GCTGAGCTTC AGGTCTCTGA CATGAGCTTT CTATCAGAGT 
     1261 TGACAGTTTT GAGACTTTCC CATAACAGAA TCCAGCTACT TGATTTAAGT GTTTTCAAGT 
     1321 TCAACCAGGA TTTAGAATAT TTGGATTTAT CTCATAATCA GTTGCAAAAG ATATCCTGCC 
     1381 ATCCTATTGT GAGTTTCAGG CATTTAGATC TCTCATTCAA TGATTTCAAG GCCCTGCCCA 
     1441 TCTGTAAGGA ATTTGGCAAC TTATCACAAC TGAATTTCTT GGGATTGAGT GCTATGAAGC 
     1501 TGCAAAAATT AGATTTGCTG CCAATTGCTC ACTTGCATCT AAGTTATATC CTTCTGGATT 
     1561 TAAGAAATTA TTATATAAAA GAAAATGAGA CAGAAAGTCT ACAAATTCTG AATGCAAAAA 
     1621 CCCTTCACCT TGTTTTTCAC CCAACTAGTT TATTCGCTAT CCAAGTGAAC ATATCAGTTA 
     1681 ATACTTTAGG GTGCTTACAA CTGACTAATA TTAAATTGAA TGATGACAAC TGTCAAGTTT 
     1741 TCATTAAATT TTTATCAGAA CTCACCAGAG GTTCAACCTT ACTGAATTTT ACCCTCAACC 
     1801 ACATAGAAAC GACTTGGAAA TGCCTGGTCA GAGTCTTTCA ATTTCTTTGG CCCAAACCTG 
     1861 TGGAATATCT CAATATTTAC AATTTAACAA TAATTGAAAG CATTCGTGAA GAAGATTTTA 
     1921 CTTATTCTAA AACGACATTG AAAGCATTGA CAATAGAACA TATCACGAAC CAAGTTTTTC 
     1981 TGTTTTCACA GACAGCTTTG TACACCGTGT TTTCTGAGAT GAACATTATG ATGTTAACCA 
     2041 TTTCAGATAC ACCTTTTATA CACATGCTGT GTCCTCATGC ACCAAGCACA TTCAAGTTTT 
     2101 TGAACTTTAC CCAGAACGTT TTCACAGATA GTATTTTTGA AAAATGTTCC ACGTTAGTTA 
     2161 AATTGGAGAC ACTTATCTTA CAAAAAAATG GATTAAAAGA CCTTTTCAAA GTAGGTCTCA 
     2221 TGACGAAGGA TATGCCTTCT TTGGAAATAC TGGATGTTAG CTGGAACTCT TTGGAATCTG 
     2281 GTAGACATAA AGAAAACTGC ACTTGGGTTG AGAGTATAGT GGTGTTAAAT TTGTCTTCAA 
     2341 ATATGCTTAC TGACTCTGTT TTCAGATGTT TACCTCCCAG GATCAAGGTA CTTGATCTTC 
     2401 ACAGCAATAA AATAAAGAGC GTTCCTAAAC AAGTCGTAAA ACTGGAAGCT TTGCAAGAAC 
     2461 TCAATGTTGC TTTCAATTCT TTAACTGACC TTCCTGGATG TGGCAGCTTT AGCAGCCTTT 
     2521 CTGTATTGAT CATTGATCAC AATTCAGTTT CCCACCCATC GGCTGATTTC TTCCAGAGCT 
     2581 GCCAGAAGAT GAGGTCAATA AAAGCAGGGG ACAATCCATT CCAATGTACC TGTGAGCTAA 
     2641 GAGAATTTGT CAAAAATATA GACCAAGTAT CAAGTGAAGT GTTAGAGGGC TGGCCTGATT 
     2701 CTTATAAGTG TGACTACCCA GAAAGTTATA GAGGAAGCCC ACTAAAGGAC TTTCACATGT 
     2761 CTGAATTATC CTGCAACATA ACTCTGCTGA TCGTCACCAT CGGTGCCACC ATGCTGGTGT 
     2821 TGGCTGTGAC TGTGACCTCC CTCTGCATCT ACTTGGATCT GCCCTGGTAT CTCAGGATGG 
     2881 TGTGCCAGTG GACCCAGACT CGGCGCAGGG CCAGGAACAT ACCCTTAGAA GAACTCCAAA 
     2941 GAAACCTCCA GTTTCATGCT TTTATTTCAT ATAGTGAACA TGATTCTGCC TGGGTGAAAA 
     3001 GTGAATTGGT ACCTTACCTA GAAAAAGAAG ATATACAGAT TTGTCTTCAT GAGAGGAACT 
     3061 TTGTCCCTGG CAAGAGCATT GTGGAAAATA TCATCAACTG CATTGAGAAG AGTTACAAGT 
     3121 CCATCTTTGT TTTGTCTCCC AACTTTGTCC AGAGTGAGTG GTGCCATTAC GAACTCTATT 
     3181 TTGCCCATCA CAATCTCTTT CATGAAGGAT CTAATAACTT AATCCTCATC TTACTGGAAC 
     3241 CCATTCCACA GAACAGCATT CCCAACAAGT ACCACAAGCT GAAGGCTCTC ATGACGCAGC 
     3301 GGACTTATTT GCAGTGGCCC AAGGAGAAAA GCAAACGTGG GCTCTTTTGG GCTAACATTA 
     3361 GAGCCGCTTT TAATATGAAA TTAACACTAG TCACTGAAAA CAATGATGTG AAATCTACGC 
     3421 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     3481 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     3541 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     3601 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     3661 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     3721 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     3781 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     3841 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     3901 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     3961 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     4021 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     4081 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     4141 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     4201 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     4261 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     4321 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     4381 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     4441 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     4501 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     4561 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     4621 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     4681 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     4741 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     4801 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     4861 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     4921 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     4981 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     5041 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     5101 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     5161 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     5221 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     5281 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     5341 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     5401 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     5461 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     5521 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     5581 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     5641 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     5701 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     5761 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     5821 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     5881 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     5941 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     6001 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     6061 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     6121 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     6181 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     6241 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     6301 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     6361 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     6421 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     6481 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     6541 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     6601 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     6661 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     6721 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     6781 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     6841 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     6901 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     6961 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     7021 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     7081 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     7141 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     7201 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     7261 GCGAATTTT
//