LOCUS       RC215568                8847 bp    DNA     circular  SYN 
DEFINITION  FRMPD4 (Myc-DDK-tagged)-Human FERM and PDZ domain containing 4 (FRMPD4)
ACCESSION   NM_014728
VERSION     NM_014728.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5004..5095 
                     /label="Myc-DDK"  
     misc_feature    5125..5710 
                     /label="PolyA signal"  
     misc_feature    5182..5201 
                     /label="Restriction Enzyme"  
     misc_feature    5986..6658 
                     /label="ColE1"  
     misc_feature    7088..7882 
                     /label="Kan/Neo"  
     misc_feature    7917..8219 
                     /label="SV40 ori"  
     misc_feature    8305..8782 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4986..4992 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4986 
                     /label="FRMPD4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATGTCTTC AGCTTTGTGA AGATTGCAAA GCTTTCGAGC CACAGGACGA 
     1081 AGTCTTCAGG CTGGCCGCCT CCCTCGGGAA CCTGGGGCTT GAGCCAGGTG CCGCCCTATG 
     1141 GATGGGAGAT GACGGCAAAC CGAGATGGGC GAGACTACTT CATCAATCAC ATGACACAGG 
     1201 CAATCCCTTT TGACGACCCT CGGTTAGAGA GCTGCCAAAT CATCCCTCCG GCTCCTCGGA 
     1261 AGGTGGAGAT GAGAAGGGAC CCCGTGCTGG GATTTGGTTT TGTGGCAGGC AGTGAAAAGC 
     1321 CAGTGGTCGT TCGCTCAGTA ACACCAGGTG GCCCCTCTGA AGGCAAGCTG ATCCCGGGAG 
     1381 ATCAGATTGT AATGATTAAT GATGAACCGG TCAGCGCTGC ACCCAGAGAG CGGGTCATCG 
     1441 ATCTGGTCAG AAGCTGCAAA GAATCGATAC TCCTCACTGT CATTCAGCCT TACCCTTCTC 
     1501 CCAAATCAGC ATTTATTAGT GCTGCAAAAA AGGCAAGATT AAAGTCCAAT CCTGTCAAAG 
     1561 TACGCTTCTC TGAGGAGGTC ATCATCAACG GCCAAGTGTC GGAAACTGTT AAGGACAACT 
     1621 CACTTCTTTT TATGCCAAAT GTTTTGAAAG TCTATCTGGA AAATGGGCAG ACCAAATCAT 
     1681 TTCGTTTTGA CTGCAGCACT TCCATTAAGG ATGTCATCTT AACCCTTCAA GAGAAGCTCT 
     1741 CCATCAAAGG CATTGAACAC TTCTCTCTCA TGCTGGAGCA GAGGACAGAA GGGGCTGGAA 
     1801 CGAAGCTGCT CTTGCTTCAT GAACAGGAGA CTCTAACTCA GGTGACACAG AGGCCCAGCT 
     1861 CCCATAAGAT GAGATGTCTT TTCCGAATTA GCTTCGTCCC AAAAGATCCA ATTGACCTTT 
     1921 TAAGGAGAGA TCCAGTTGCT TTCGAGTATC TCTATGTTCA GAGTTGTAAC GATGTGGTTC 
     1981 AGGAGCGATT TGGGCCGGAG CTGAAATATG ACATAGCCCT GCGGCTGGCC GCATTACAAA 
     2041 TGTACATTGC AACCGTTACC ACCAAGCAAA CGCAGAAAAT CTCCCTCAAA TACATCGAAA 
     2101 AAGAATGGGG ATTAGAGACT TTTCTTCCCT CTGCTGTGCT GCAAAGCATG AAAGAGAAGA 
     2161 ACATAAAGAA AGCACTTTCA CACCTTGTCA AAGCAAATCA AAACTTGGTA CCACCGGGTA 
     2221 AAAAGCTCTC TGCACTACAA GCCAAGGTCC ATTATCTCAA GTTCCTCAGT GACCTACGAT 
     2281 TGTATGGGGG CCGTGTGTTC AAGGCAACAT TAGTGCAGGC AGAAAAGCGC TCGGAAGTGA 
     2341 CTCTCCTGGT TGGGCCCCGG TATGGCATAA GCCATGTCAT CAACACCAAA ACCAATCTGG 
     2401 TGGCTCTTTT AGCCGACTTT AGCCACGTCA ACAGGATCGA AATGTTTTCC GAGGAGGAGA 
     2461 GCTTGGTGCG GGTAGAACTC CACGTGCTAG ATGTGAAGCC TATCACGCTT CTGATGGAAT 
     2521 CCTCAGATGC CATGAACCTG GCCTGCTTGA CGGCTGGATA CTACCGGCTG CTTGTTGATT 
     2581 CCAGGAGGTC GATATTTAAC ATGGCCAACA AGAAAAACAC AGCGACCCAG GAAACAGGAC 
     2641 CTGAAAACAA GGGGAAGCAT AACCTCCTTG GCCCAGATTG GAACTGTATA CCCCAAATGA 
     2701 CCACCTTTAT TGGCGAAGGG GAACAAGAAG CCCAGATAAC ATACATAGAT TCAAAGCAGA 
     2761 AGACGGTGGA GATCACAGAC AGCACCATGT GTCCAAAAGA GCACCGGCAC TTGTACATAG 
     2821 ACAATGCCTA TAGTTCAGAT GGACTTAACC AGCAGCTGAG CCAGCCCGGG GAGGCCCCCT 
     2881 GTGAGGCAGA CTACAGAAGT CTAGCTCAGC GGTCCCTATT GACCCTCTCA GGACCAGAAA 
     2941 CTCTGAAGAA AGCACAGGAA TCTCCGAGAG GAGCTAAAGT GTCCTTTATT TTTGGAGACT 
     3001 TCGCCTTGGA TGATGGTATT AGTCCCCCAA CCCTTGGCTA TGAAACGCTA CTAGATGAGG 
     3061 GTCCTGAAAT GCTGGAGAAG CAGAGAAATC TCTACATTGG CAGTGCCAAT GACATGAAGG 
     3121 GCCTGGATCT CACTCCAGAG GCAGAGGGCA TCCAGTTTGT GGAAAATTCT GTTTATGCAA 
     3181 ACATAGGCGA TGTGAAGAGC TTCCAGGCCG CGGAGGGGAT CGAGGAACCC CTCTTGCATG 
     3241 ACATCTGTTA TGCAGAAAAC ACTGATGACG CGGAGGACGA GGACGAGGTG AGCTGCGAGG 
     3301 AGGACCTCGT GGTGGGGGAG ATGAACCAGC CGGCCATCCT CAACCTGTCT GGGTCAAGCG 
     3361 ATGACATCAT TGACCTCACA TCCCTGCCCC CTCCAGAAGG TGATGACAAT GAGGATGACT 
     3421 TCCTGTTGCG TTCCTTGAAC ATGGCCATTG CCGCACCCCC ACCTGGCTTT AGAGACAGTT 
     3481 CAGATGAAGA GGACTCTCAG AGCCAGGCAG CTTCCTTCCC CGAGGACAAG GAGAAAGGCA 
     3541 GCAGCCTGCA AAATGATGAG ATCCCCGTGT CCCTCATTGA CGCTGTGCCC ACCAGCGCCG 
     3601 AAGGCAAGTG TGAGAAGGGA CTGGATAATG CCGTCGTCTC CACGCTGGGA GCTCTAGAGG 
     3661 CTCTATCCGT GTCAGAAGAA CAGCAGACCA GTGACAATTC AGGTGTAGCC ATCTTGCGGG 
     3721 CTTATAGTCC TGAGTCTTCG TCAGACTCGG GCAATGAAAC TAACTCTTCT GAAATGACTG 
     3781 AGAGTTCTGA ACTGGCCACA GCACAAAAAC AGTCAGAAAA CCTCTCCCGC ATGTTCTTGG 
     3841 CCACTCACGA AGGCTACCAC CCCCTTGCAG AAGAGCAGAC CGAGTTCCCG GCCTCCAAGA 
     3901 CCCCCGCTGG GGGCTTGCCT CCAAAGTCCT CGCACGCCCT GGCTGCTAGG CCAGCAACCG 
     3961 ACCTCCCGCC CAAAGTTGTG CCTTCCAAGC AGTTACTTCA CTCAGACCAC ATGGAGATGG 
     4021 AGCCTGAAAC TATGGAGACT AAGTCGGTCA CTGACTATTT TAGCAAACTG CACATGGGGT 
     4081 CGGTGGCATA CTCCTGCACT AGCAAAAGGA AAAGCAAGCT GGCCGATGGT GAGGGGAAGG 
     4141 CACCCCCTAA TGGGAACACA ACAGGAAAAA AACAGCAGGG GACCAAAACG GCAGAGATGG 
     4201 AGGAGGAGGC CAGTGGTAAA TTTGGTACTG TGTCTTCACG AGACAGTCAA CACCTGAGCA 
     4261 CTTTTAATCT GGAGAGAACT GCCTTTCGCA AGGACAGTCA AAGATGGTAT GTGGCCACTG 
     4321 AAGGTGGGAT GGCTGAAAAA AGTGGATTAG AAGCAGCAAC AGGGAAAACC TTTCCAAGAG 
     4381 CTTCTGGTCT TGGGGCAAGG GAGGCCGAAG GGAAGGAAGA AGGAGCTCCT GATGGAGAAA 
     4441 CCAGTGATGG CTCAGGACTT GGTCAAGGGG ACCGCTTCTT AACTGACGTG ACCTGTGCAT 
     4501 CTTCAGCCAA AGACTTAGAT AACCCAGAGG ACGCTGACTC GTCCACCTGC GACCATCCTT 
     4561 CCAAGCTTCC TGAGGCTGAT GAGAGTGTGG CCCGCCTTTG TGACTACCAC TTGGCCAAGC 
     4621 GGATGTCATC ACTGCAAAGC GAGGGCCATT TTTCTCTGCA GAGCTCCCAA GGCTCTTCAG 
     4681 TGGATGCAGG CTGTGGCACA GGCAGCAGTG GCAGTGCCTG TGCCACACCC GTGGAGTCGC 
     4741 CGCTCTGCCC CTCCCTGGGG AAGCACTTGA TTCCTGACGC TTCTGGGAAA GGCGTGAATT 
     4801 ACATTCCTTC AGAGGAGAGA GCCCCTGGGC TTCCCAACCA CGGAGCCACC TTTAAGGAAC 
     4861 TGCACCCACA GACAGAAGGG ATGTGTCCAC GGATGACAGT GCCTGCTCTG CACACAGCCA 
     4921 TTAACACCGA ACCCCTGTTT GGCACATTGA GAGATGGATG CCATCGGCTC CCCAAGATTA 
     4981 AGGAAACCAC AGTGACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     5041 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     5101 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     5161 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     5221 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     5281 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     5341 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     5401 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     5461 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     5521 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     5581 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     5641 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     5701 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     5761 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     5821 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     5881 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     5941 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     6001 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     6061 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     6121 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     6181 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     6241 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     6301 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     6361 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     6421 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     6481 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     6541 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     6601 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     6661 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     6721 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     6781 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     6841 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     6901 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     6961 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     7021 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     7081 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     7141 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     7201 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     7261 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     7321 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     7381 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     7441 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     7501 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     7561 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     7621 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     7681 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     7741 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     7801 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     7861 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     7921 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     7981 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     8041 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     8101 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     8161 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     8221 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     8281 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     8341 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     8401 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     8461 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     8521 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     8581 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     8641 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     8701 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     8761 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     8821 ATTTAACAAA AATTTAACGC GAATTTT
//