LOCUS       RC215546                8058 bp    DNA     circular  SYN 
DEFINITION  PLEKHM1 (Myc-DDK-tagged)-Human pleckstrin homology domain containing, family M (with RUN domain) member 1 (PLEKHM1), transcript variant 1
ACCESSION   NM_014798
VERSION     NM_014798.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4213..4304 
                     /label="Myc-DDK"  
     misc_feature    4334..4919 
                     /label="PolyA signal"  
     misc_feature    4391..4410 
                     /label="Restriction Enzyme"  
     misc_feature    5195..5867 
                     /label="ColE1"  
     misc_feature    6297..7091 
                     /label="Kan/Neo"  
     misc_feature    7126..7428 
                     /label="SV40 ori"  
     misc_feature    7514..7991 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4188..4195 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4188 
                     /label="PLEKHM1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTTTCAGTG GTGGAGAATG GACTGGACCC CCAGGCTGCC ATCCCGGTCA 
     1081 TCAAGAAGAA GCTGGTGGGA TCCGTGAAGG CCTTGCAGAA GCAGTACGTG TCCCTGGACA 
     1141 CGGTGGTCAC TAGTGAAGAC GGAGATGCCA ACACCATGTG CAGCGCCCTG GAGGCCGTAT 
     1201 TTATCCATGG CCTGCACGCC AAGCACATCC GAGCTGAGGC CGGAGGAAAA AGGAAGAAAA 
     1261 GTGCCCACCA GAAGCCTCTG CCCCAGCCTG TCTTCTGGCC CCTCCTGAAA GCTGTCACCC 
     1321 ACAAACACAT CATCTCAGAG TTGGAGCACC TGACGTTTGT CAACACGGAT GTGGGCCGCT 
     1381 GCCGGGCATG GCTGCGGCTG GCCCTGAACG ATGGCCTGAT GGAGTGCTAC CTGAAGCTGC 
     1441 TGCTGCAGGA GCAGGCCCGC TTGCATGAGT ACTACCAGCC CACCGCCCTG CTCCGGGATG 
     1501 CTGAGGAGGG CGAGTTCCTC CTTAGCTTCC TGCAGGGCCT CACGTCCTTG TCCTTCGAAC 
     1561 TCTCCTACAA GTCTGCCATC TTAAATGAGT GGACGCTCAC CCCATTGGCC CTGTCTGGGC 
     1621 TTTGCCCGCT TTCTGAGCTG GACCCTCTCT CTACCTCTGG TGCAGAACTA CAGCGGAAGG 
     1681 AATCTCTGGA TTCCATTTCC CATTCTTCAG GCTCTGAAGA CATCGAAGTC CATCACTCGG 
     1741 GCCATAAGAT ACGGAGGAAC CAGAAGCTGA CTGCCTCCTC CCTCAGCCTG GACACGGCCA 
     1801 GTTCATCCCA GCTGTCCTGC AGCCTAAACT CTGATAGCTG CTTACTCCAA GAGAATGGCT 
     1861 CCAAGAGTCC AGACCATTGC GAGGAGCCCA TGTCCTGTGA CTCAGACCTG GGCACAGCAA 
     1921 ATGCTGAGGA CTCAGACCGG TCTCTGCAAG AGGTATTGTT GGAATTCAGC AAAGCCCAGG 
     1981 TAAACTCTGT GCCAACCAAC GGACTGAGCC AAGAAACAGA GATCCCCACA CCACAGGCCT 
     2041 CGCTCTCCCT CCATGGCCTC AACACCAGCA CATACCTGCA CTGTGAGGCA CCTGCAGAGC 
     2101 CCCTTCCTGC CCAGGCAGCC TCTGGAACTC AAGATGGTGT CCACGTGCAG GAGCCGCGTC 
     2161 CCCAGGCGCC CAGCCCCCTG GACTTACAGC AGCCTGTAGA GAGCACCTCA GGCCAGCAGC 
     2221 CTTCTAGTAC TGTCAGCGAG ACAGCCAGAG AAGTGGGCCA AGGGAATGGC CTGCAGAAGG 
     2281 CCCAGGCTCA TGACGGAGCT GGTCTGAAGC TGGTAGTTTC CTCACCCACC AGTCCGAAAA 
     2341 ACAAGAGCTG GATCTCAGAG GATGACTTCT ACCGGCCTTC CCGGGAGCAA CCCCTGGAGA 
     2401 GTGCTTCAGA CCACCCAATA GCTTCTTACA GGGGGACTCC AGGGTCAAGG CCTGGTCTCC 
     2461 ACAGGCATTT TTCTCAAGAA CCAAGAAAAA ACTGCTCCCT GGGGGCGTTA GACCAAGCGT 
     2521 GTGTACCTTC CCCAGGAAGA AGGCAAGCCC AGGCAGCCCC ATCCCAGGGG CATAAGAGCT 
     2581 TCCGGGTGGT ACACCGGAGA CAGATGGGAC TGTCCAACCC ATTCCGGGGT CTCATGAAGC 
     2641 TGGGCACCGT GGAGCGGCGG GGGGCAATGG GCATCTGGAA GGAGCTCTTC TGCGAGCTCT 
     2701 CCCCGCTGGA GTTCCGCCTC TACCTGAGCA ACGAGGAGCA CACCTGTGTG GAGAACTGCT 
     2761 CGCTGCTTCG CTGTGAGTCT GTGGGGCCAG CCCATAGTGA TGGGCGCTTT GAGCTGGTCT 
     2821 TCTCTGGCAA GAAGCTGGCC CTGCGCGCCT CCTCCCAGGA CGAAGCTGAG GACTGGCTGG 
     2881 ACCGGGTGCG GGAGGCCCTG CAGAAGGTCC GGCCTCAGCA GGAGGATGAG TGGGTGAACG 
     2941 TGCAGTACCC AGACCAGCCT GAGGAACCCC CCGAGGCGCC CCAGGGCTGC CTCTCTCCCT 
     3001 CAGACCTGCT CTCGGAGCCC GCGGCCCTCC AGGGCACACA GTTTGACTGG TCGTCCGCCC 
     3061 AGGTTCCAGA GCCAGATGCC ATCAAGGAGT CCCTGCTGTA CTTGTACATG GACAGGACCT 
     3121 GGATGCCCTA TATATTTTCT CTGTCCTTGG AGGCTCTGAA ATGTTTCCGC ATCAGGAACA 
     3181 ATGAGAAGAT GCTGAGTGAC AGCCACGGCG TGGAGACCAT CCGGGACATC CTGCCAGACA 
     3241 CCAGCCTTGG GGGCCCATCC TTCTTCAAAA TCATCACGGC CAAGGCTGTC CTGAAGCTGC 
     3301 AGGCCGGAAA CGCCGAGGAA GCCGCCCTGT GGAGGGATCT GGTCCGCAAA GTCCTGGCAT 
     3361 CCTACTTGGA GACAGCCGAG GAGGCGGTGA CCCTGGGCGG GAGCCTGGAT GAAAACTGTC 
     3421 AGGAGGTGCT GAAATTTGCC ACCCGGGAGA ATGGCTTCCT GCTGCAGTAC CTGGTGGCTA 
     3481 TCCCCATGGA GAAAGGCCTT GACTCCCAAG GCTGCTTCTG CGCAGGCTGC TCCCGGCAGA 
     3541 TCGGCTTCTC CTTTGTACGA CCCAAGCTCT GTGCCTTCTC TGGCCTCTAT TACTGTGACA 
     3601 TCTGCCACCA AGACGATGCC TCAGTGATTC CGGCCAGGAT CATCCACAAC TGGGACCTCA 
     3661 CCAAGCGCCC GATCTGCAGG CAGGCCCTGA AGTTTCTGAC ACAGATCCGG GCCCAGCCCC 
     3721 TCATCAACCT GCAGATGGTG AACGCGTCTC TGTACGAGCA TGTGGAGCGG ATGCACCTCA 
     3781 TTGGGAGGAG ACGGGAGCAG CTGAAGCTCC TGGGGGATTA CCTGGGCCTG TGCCGGAGTG 
     3841 GCGCCCTGAA GGAGCTCAGC AAGAGGCTCA ACCACAGGAA TTATCTCTTG GAATCTCCGC 
     3901 ATAGGTTCAG TGTTGCTGAC CTCCAACAGA TCGCAGACGG GGTGTATGAA GGATTCCTCA 
     3961 AGGCCCTGAT TGAATTTGCC TCCCAGCATG TCTACCACTG CGACCTGTGC ACCCAGCGCG 
     4021 GCTTCATCTG CCAGATCTGC CAGCACCACG ACATCATCTT CCCCTTTGAG TTTGACACCA 
     4081 CAGTCAGGTG TGCCGAGTGC AAGACCGTCT TCCACCAGAG CTGCCAGGCT GTGGTGAAGA 
     4141 AGGGCTGCCC CCGCTGTGCC CGCCGGCGCA AGTACCAGGA ACAGAACATT TTCGCCAGCG 
     4201 GACCGACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     4261 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     4321 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4381 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     4441 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     4501 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     4561 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     4621 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     4681 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     4741 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     4801 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     4861 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     4921 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     4981 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     5041 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     5101 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     5161 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     5221 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     5281 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     5341 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     5401 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     5461 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     5521 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     5581 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     5641 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     5701 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     5761 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     5821 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     5881 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     5941 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     6001 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     6061 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     6121 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     6181 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     6241 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     6301 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     6361 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     6421 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     6481 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     6541 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     6601 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     6661 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     6721 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     6781 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     6841 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     6901 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     6961 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     7021 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     7081 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     7141 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     7201 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     7261 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     7321 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7381 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7441 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     7501 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     7561 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     7621 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     7681 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     7741 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     7801 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     7861 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     7921 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     7981 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     8041 AAATTTAACG CGAATTTT
//