LOCUS       RC215529                6747 bp    DNA     circular  SYN 
DEFINITION  DMD (Myc-DDK-tagged)-Human dystrophin (DMD), transcript variant Dp71ab
ACCESSION   NM_004018
VERSION     NM_004018.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2904..2995 
                     /label="Myc-DDK"  
     misc_feature    3025..3610 
                     /label="PolyA signal"  
     misc_feature    3082..3101 
                     /label="Restriction Enzyme"  
     misc_feature    3886..4558 
                     /label="ColE1"  
     misc_feature    4988..5782 
                     /label="Kan/Neo"  
     misc_feature    5817..6119 
                     /label="SV40 ori"  
     misc_feature    6205..6682 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2886..2892 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2886 
                     /label="DMD"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGGGAACAG CTCAAAGGCC ACGAGACTCA AACAACTTGC TGGGACCATC 
     1081 CCAAAATGAC AGAGCTCTAC CAGTCTTTAG CTGACCTGAA TAATGTCAGA TTCTCAGCTT 
     1141 ATAGGACTGC CATGAAACTC CGAAGACTGC AGAAGGCCCT TTGCTTGGAT CTCTTGAGCC 
     1201 TGTCAGCTGC ATGTGATGCC TTGGACCAGC ACAACCTCAA GCAAAATGAC CAGCCCATGG 
     1261 ATATCCTGCA GATTATTAAT TGTTTGACCA CTATTTATGA CCGCCTGGAG CAAGAGCACA 
     1321 ACAATTTGGT CAACGTCCCT CTCTGCGTGG ATATGTGTCT GAACTGGCTG CTGAATGTTT 
     1381 ATGATACGGG ACGAACAGGG AGGATCCGTG TCCTGTCTTT TAAAACTGGC ATCATTTCCC 
     1441 TGTGTAAAGC ACATTTGGAA GACAAGTACA GATACCTTTT CAAGCAAGTG GCAAGTTCAA 
     1501 CAGGATTTTG TGACCAGCGC AGGCTGGGCC TCCTTCTGCA TGATTCTATC CAAATTCCAA 
     1561 GACAGTTGGG TGAAGTTGCA TCCTTTGGGG GCAGTAACAT TGAGCCAAGT GTCCGGAGCT 
     1621 GCTTCCAATT TGCTAATAAT AAGCCAGAGA TCGAAGCGGC CCTCTTCCTA GACTGGATGA 
     1681 GACTGGAACC CCAGTCCATG GTGTGGCTGC CCGTCCTGCA CAGAGTGGCT GCTGCAGAAA 
     1741 CTGCCAAGCA TCAGGCCAAA TGTAACATCT GCAAAGAGTG TCCAATCATT GGATTCAGGT 
     1801 ACAGGAGTCT AAAGCACTTT AATTATGACA TCTGCCAAAG CTGCTTTTTT TCTGGTCGAG 
     1861 TTGCAAAAGG CCATAAAATG CACTATCCCA TGGTGGAATA TTGCACTCCG ACTACATCAG 
     1921 GAGAAGATGT TCGAGACTTT GCCAAGGTAC TAAAAAACAA ATTTCGAACC AAAAGGTATT 
     1981 TTGCGAAGCA TCCCCGAATG GGCTACCTGC CAGTGCAGAC TGTCTTAGAG GGGGACAACA 
     2041 TGGAAACGCC TGCCTCGTCC CCTCAGCTTT CACACGATGA TACTCATTCA CGCATTGAAC 
     2101 ATTATGCTAG CAGGCTAGCA GAAATGGAAA ACAGCAATGG ATCTTATCTA AATGATAGCA 
     2161 TCTCTCCTAA TGAGAGCATA GATGATGAAC ATTTGTTAAT CCAGCATTAC TGCCAAAGTT 
     2221 TGAACCAGGA CTCCCCCCTG AGCCAGCCTC GTAGTCCTGC CCAGATCTTG ATTTCCTTAG 
     2281 AGAGTGAGGA AAGAGGGGAG CTAGAGAGAA TCCTAGCAGA TCTTGAGGAA GAAAACAGGA 
     2341 ATCTGCAAGC AGAATATGAC CGTCTAAAGC AGCAGCACGA ACATAAAGGC CTGTCCCCAC 
     2401 TGCCGTCCCC TCCTGAAATG ATGCCCACCT CTCCCCAGAG TCCCCGGGAT GCTGAGCTCA 
     2461 TTGCTGAGGC CAAGCTACTG CGTCAACACA AAGGCCGCCT GGAAGCCAGG ATGCAAATCC 
     2521 TGGAAGACCA CAATAAACAG CTGGAGTCAC AGTTACACAG GCTAAGGCAG CTGCTGGAGC 
     2581 AACCCCAGGC AGAGGCCAAA GTGAATGGCA CAACGGTGTC CTCTCCTTCT ACCTCTCTAC 
     2641 AGAGGTCCGA CAGCAGTCAG CCTATGCTGC TCCGAGTGGT TGGCAGTCAA ACTTCGGACT 
     2701 CCATGGGTGA GGAAGATCTT CTCAGTCCTC CCCAGGACAC AAGCACAGGG TTAGAGGAGG 
     2761 TGATGGAGCA ACTCAACAAC TCCTTCCCTA GTTCAAGAGG ACACAATGTA GGAAGTCTTT 
     2821 TCCACATGGC AGATGATTTG GGCAGAGCGA TGGAGTCCTT AGTATCAGTC ATGACAGATG 
     2881 AAGAAGGAGC AGAAACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     2941 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     3001 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     3061 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     3121 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     3181 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     3241 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     3301 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     3361 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     3421 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     3481 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     3541 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     3601 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     3661 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     3721 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     3781 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     3841 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     3901 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     3961 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     4021 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     4081 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     4141 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     4201 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     4261 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     4321 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     4381 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     4441 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     4501 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     4561 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     4621 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     4681 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     4741 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     4801 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     4861 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     4921 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     4981 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     5041 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     5101 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     5161 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     5221 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     5281 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     5341 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     5401 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     5461 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     5521 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     5581 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     5641 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     5701 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     5761 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     5821 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     5881 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     5941 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     6001 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     6061 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     6121 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     6181 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     6241 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     6301 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     6361 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     6421 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     6481 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     6541 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     6601 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     6661 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     6721 ATTTAACAAA AATTTAACGC GAATTTT
//