LOCUS       RC215526                9327 bp    DNA     circular  SYN 
DEFINITION  PZP (Myc-DDK-tagged)-Human pregnancy-zone protein (PZP)
ACCESSION   NM_002864
VERSION     NM_002864.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5484..5575 
                     /label="Myc-DDK"  
     misc_feature    5605..6190 
                     /label="PolyA signal"  
     misc_feature    5662..5681 
                     /label="Restriction Enzyme"  
     misc_feature    6466..7138 
                     /label="ColE1"  
     misc_feature    7568..8362 
                     /label="Kan/Neo"  
     misc_feature    8397..8699 
                     /label="SV40 ori"  
     misc_feature    8785..9262 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5466..5472 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5466 
                     /label="PZP"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCGGAAAGAC AGACTTCTTC ATTTATGTCT TGTGCTACTT CTTATCCTGC 
     1081 TTTCTGCCAG TGACTCAAAC TCTACAGAAC CGCAGTATAT GGTGCTGGTC CCCTCCCTGC 
     1141 TCCACACTGA GGCCCCTAAG AAGGGCTGTG TCCTTCTGAG CCACCTGAAT GAGACAGTGA 
     1201 CTGTAAGTGC TTCCTTGGAG TCTGGCAGGG AAAACAGGAG CCTCTTCACT GACCTGGTGG 
     1261 CGGAGAAGGA CTTATTCCAC TGTGTCTCCT TCACTCTCCC AAGGATCTCA GCCTCTTCAG 
     1321 AGGTGGCATT CCTTAGCATC CAGATAAAGG GGCCTACGCA AGATTTCAGG AAGAGGAACA 
     1381 CAGTTCTGGT ACTGAACACC CAAAGTCTGG TCTTTGTCCA GACAGACAAA CCCATGTATA 
     1441 AACCAGGACA GACAGTAAGA TTCCGTGTTG TCTCCGTGGA TGAAAATTTT CGCCCTCGAA 
     1501 ATGAACTGAT TCCACTGATA TACCTTGAGA ACCCAAGAAG AAATCGAATT GCACAATGGC 
     1561 AGAGTCTCAA GCTAGAAGCT GGCATCAATC AGTTGTCCTT TCCCCTCTCA TCAGAGCCCA 
     1621 TTCAGGGCTC CTACAGGGTG GTGGTACAGA CAGAATCAGG TGGAAGGATA CAGCACCCCT 
     1681 TCACCGTGGA GGAATTTGTG CTTCCCAAGT TTGAGGTCAA AGTTCAGGTG CCAAAGATAA 
     1741 TCAGTATCAT GGATGAAAAA GTGAACATAA CAGTCTGTGG AGAATACACT TATGGGAAGC 
     1801 CTGTCCCAGG ACTTGCAACT GTGAGCCTGT GTAGAAAATT ATCTCGTGTT CTTAATTGTG 
     1861 ACAAGCAGGA GGTCTGTGAG GAATTCAGTC AACAGCTTAA CAGCAATGGC TGCATCACCC 
     1921 AACAAGTACA CACCAAAATG CTCCAGATTA CAAATACGGG CTTTGAAATG AAGCTTAGAG 
     1981 TGGAAGCCAG GATCAGAGAA GAGGGGACAG ACCTGGAAGT CACTGCAAAC AGGATCAGTG 
     2041 AAATCACAAA CATTGTATCC AAACTCAAAT TCGTGAAAGT GGATTCACAC TTTAGACAAG 
     2101 GAATCCCCTT TTTTGCACAG GTGCTTCTGG TGGATGGAAA AGGTGTGCCC ATCCCCAATA 
     2161 AACTCTTCTT CATCTCTGTG AATGACGCCA ATTATTACTC CAATGCAACC ACCAATGAGC 
     2221 AGGGTCTTGC ACAGTTTTCA ATCAATACTA CCAGTATCTC GGTTAATAAA CTTTTTGTCC 
     2281 GGGTTTTCAC TGTGCATCCC AACTTGTGTT TTCACTATTC ATGGGTAGCA GAAGACCACC 
     2341 AGGGTGCTCA GCACACTGCA AATCGTGTTT TCTCCTTAAG TGGAAGTTAC ATTCACCTGG 
     2401 AGCCTGTGGC TGGTACCCTG CCCTGTGGCC ACACGGAGAC TATCACGGCA CACTATACAC 
     2461 TGAATAGACA GGCCATGGGA GAGTTATCGG AGCTCAGTTT CCATTACCTG ATCATGGCTA 
     2521 AGGGAGTCAT CGTCAGATCT GGAACCCACA CTCTGCCTGT GGAGTCAGGA GACATGAAAG 
     2581 GCAGTTTTGC CTTATCCTTC CCTGTGGAGT CAGACGTTGC CCCCATTGCA CGAATGTTCA 
     2641 TCTTTGCCAT TTTACCAGAT GGAGAAGTTG TTGGAGACTC TGAAAAATTT GAGATTGAAA 
     2701 ACTGTCTAGC CAACAAGGTG GATTTGAGCT TCAGCCCAGC ACAAAGTCCC CCAGCCTCAC 
     2761 ATGCCCACCT GCAAGTAGCA GCTGCTCCGC AGTCCCTCTG TGCCCTTCGT GCTGTGGACC 
     2821 AAAGTGTGCT GCTCATGAAG CCTGAGGCTG AGCTCTCTGT GTCCTCAGTA TATAATCTGC 
     2881 TAACTGTGAA GGATCTCACC AATTTTCCTG ACAATGTGGA CCAGCAGGAG GAAGAACAAG 
     2941 GACACTGTCC CCGTCCTTTC TTCATTCATA ATGGAGCCAT CTATGTTCCC TTATCAAGTA 
     3001 ATGAAGCAGA TATTTATAGC TTCCTCAAGG GGATGGGATT GAAGGTGTTC ACTAACTCAA 
     3061 AAATCCGAAA ACCAAAGTCG TGTTCAGTCA TCCCTTCCGT GTCTGCAGGA GCAGTAGGTC 
     3121 AAGGATACTA TGGAGCAGGT CTAGGAGTAG TAGAGAGACC ATATGTTCCT CAATTAGGCA 
     3181 CATATAATGT GATACCCTTA AATAATGAAC AAAGTTCAGG GCCAGTCCCT GAAACGGTGC 
     3241 GAAGCTATTT TCCTGAGACT TGGATCTGGG AGTTGGTGGC AGTGAACTCA TCAGGTGTGG 
     3301 CTGAGGTAGG AGTAACAGTC CCTGACACCA TCACCGAGTG GAAGGCAGGG GCCTTCTGCC 
     3361 TGTCCGAAGA TGCTGGACTT GGTATCTCTT CCACTGCCTC TCTCCGAGCC TTCCAGCCCT 
     3421 TCTTTGTGGA GCTCACAATG CCTTACTCTG TGATTCGTGG AGAGGTCTTC ACACTCAAGG 
     3481 CCACGGTCCT AAACTACCTT CCCAAATGCA TCCGGGTCAG TGTGCAGCTG AAAGCCTCTC 
     3541 CAGCCTTCCT AGCTTCCCAA AATACAAAGG GAGAAGAATC CTATTGTATC TGTGGAAATG 
     3601 AGAGACAAAC CTTGTCTTGG ACAGTGACTC CTAAAACTCT GGGGAATGTG AACTTCTCAG 
     3661 TGAGTGCAGA GGCAATGCAG TCCTTAGAAC TCTGTGGAAA TGAGGTTGTT GAGGTCCCTG 
     3721 AGATTAAAAG AAAAGACACA GTCATCAAAA CCCTGTTGGT GGAGGCTGAA GGTATTGAGC 
     3781 AAGAAAAGAC TTTCAGTTCT ATGACCTGTG CCTCAGGTGC TAATGTGTCT GAGCAGTTGT 
     3841 CCTTGAAGCT CCCATCAAAT GTGGTCAAAG AATCTGCCAG AGCTTCTTTC TCAGTTCTGG 
     3901 GTGACATATT AGGTTCTGCT ATGCAAAATA TACAAAATCT CCTCCAGATG CCATATGGCT 
     3961 GTGGAGAACA GAACATGGTC CTATTTGCTC CTAACATCTA TGTCTTGAAC TATCTGAATG 
     4021 AAACCCAGCA GCTGACGCAG GAGATCAAGG CCAAGGCCGT TGGCTATCTC ATCACTGGTT 
     4081 ACCAGAGACA GCTGAACTAC AAACACCAAG ATGGCTCCTA CAGCACCTTT GGGGAACGAT 
     4141 ATGGCAGGAA CCAGGGCAAC ACTTGGCTCA CAGCTTTTGT ACTGAAGACT TTCGCCCAGG 
     4201 CTCGATCCTA CATCTTCATT GATGAAGCAC ACATTACCCA ATCTCTCACG TGGCTCTCCC 
     4261 AGATGCAGAA GGACAATGGC TGTTTCAGGA GCTCTGGGTC ACTGCTCAAC AATGCCATAA 
     4321 AGGGAGGTGT AGAAGATGAA GCGACCCTCT CCGCCTATGT TACTATTGCC CTTCTGGAAA 
     4381 TTCCTCTCCC AGTCACTAAC CCTATTGTTC GCAATGCCCT GTTCTGCCTG GAGTCAGCCT 
     4441 GGAATGTAGC AAAGGAGGGG ACCCATGGGA GCCATGTCTA CACCAAGGCA TTGCTGGCCT 
     4501 ATGCTTTTTC CCTACTGGGA AAGCAAAATC AGAATAGAGA AATACTGAAC TCACTTGATA 
     4561 AGGAAGCTGT GAAAGAAGAC AACCTCGTCC ATTGGGAGCG CCCTCAGAGA CCCAAGGCAC 
     4621 CAGTGGGGCA TCTTTACCAA ACCCAGGCTC CCTCTGCTGA GGTGGAGATG ACATCCTATG 
     4681 TGCTCCTCGC TTATCTCACG GCCCAGCCAG CCCCCACCTC AGGGGACCTG ACCTCTGCAA 
     4741 CTAACATTGT GAAGTGGATC ATGAAGCAGC AGAACGCCCA AGGTGGTTTC TCCTCCACCC 
     4801 AGGACACAGT GGTGGCTCTC CATGCCCTGT CCAGGTATGG AGCAGCCACT TTCACCAGAA 
     4861 CTGAGAAAAC TGCACAGGTC ACCGTTCAGG ATTCACAGAC CTTTTCTACA AATTTCCAAG 
     4921 TAGACAACAA CAACCTCCTA TTACTGCAGC AGATCTCATT GCCAGAGCTC CCTGGAGAAT 
     4981 ATGTCATAAC AGTAACTGGG GAAAGATGTG TGTATCTTCA GACATCCATG AAATACAATA 
     5041 TTCTTCCAGA GAAAGAGGAC TCCCCATTTG CTTTAAAAGT GCAGACTGTG CCCCAAACTT 
     5101 GCGATGGACA CAAAGCCCAC ACCAGCTTTC AGATCTCACT GACCATCAGT TACACAGGAA 
     5161 ACCGTCCTGC TTCCAATATG GTGATTGTTG ATGTAAAGAT GGTATCTGGT TTTATTCCCC 
     5221 TGAAACCAAC AGTAAAAATG CTTGAAAGAT CTAGCTCTGT GAGCCGGACA GAAGTGAGCA 
     5281 ACAACCATGT CCTCATTTAT GTGGAACAGG TGACAAATCA GACGCTAAGT TTTTCCTTCA 
     5341 TGGTTCTGCA AGACATCCCA GTAGGAGACT TGAAGCCAGC AATTGTTAAA GTCTATGATT 
     5401 ACTATGAGAC AGATGAGTCT GTGGTTGCTG AGTATATCGC CCCCTGCAGC ACAGATACAG 
     5461 AGCATGGAAA TGTTACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     5521 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     5581 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     5641 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     5701 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     5761 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     5821 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     5881 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     5941 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     6001 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     6061 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     6121 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     6181 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     6241 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     6301 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     6361 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     6421 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     6481 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     6541 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     6601 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     6661 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     6721 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     6781 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     6841 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     6901 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     6961 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     7021 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     7081 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     7141 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     7201 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     7261 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     7321 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     7381 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     7441 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     7501 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     7561 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     7621 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     7681 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     7741 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     7801 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     7861 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     7921 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     7981 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     8041 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     8101 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     8161 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     8221 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     8281 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     8341 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     8401 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     8461 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     8521 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     8581 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     8641 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     8701 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     8761 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     8821 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     8881 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     8941 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     9001 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     9061 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     9121 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     9181 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     9241 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     9301 ATTTAACAAA AATTTAACGC GAATTTT
//