LOCUS       RC215396                9114 bp    DNA     circular  SYN 
DEFINITION  EEA1 (Myc-DDK-tagged)-Human early endosome antigen 1 (EEA1)
ACCESSION   NM_003566
VERSION     NM_003566.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5271..5362 
                     /label="Myc-DDK"  
     misc_feature    5392..5977 
                     /label="PolyA signal"  
     misc_feature    5449..5468 
                     /label="Restriction Enzyme"  
     misc_feature    6253..6925 
                     /label="ColE1"  
     misc_feature    7355..8149 
                     /label="Kan/Neo"  
     misc_feature    8184..8486 
                     /label="SV40 ori"  
     misc_feature    8572..9049 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5253..5259 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5253 
                     /label="EEA1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTTAAGGAGG ATTTTACAGA GGACTCCTGG GAGAGTTGGC TCTCAAGGTT 
     1081 CTGATTTAGA TTCATCAGCA ACTCCTATAA ACACAGTGGA CGTCAATAAT GAAAGCTCTT 
     1141 CAGAGGGTTT CATATGTCCC CAGTGTATGA AATCTCTTGG ATCTGCTGAT GAACTTTTCA 
     1201 AACATTATGA AGCTGTTCAT GATGCTGGTA ATGACTCAGG TCATGGAGGA GAGTCTAATC 
     1261 TTGCTTTGAA GCGAGATGAT GTAACACTGC TCAGACAAGA GGTCCAAGAC CTACAGGCTT 
     1321 CACTTAAGGA AGAAAAATGG TACTCGGAAG AATTAAAGAA GGAATTAGAA AAATATCAAG 
     1381 GGCTGCAGCA GCAAGAGGCC AAACCTGATG GGTTGGTGAC TGATTCATCA GCAGAACTAC 
     1441 AGTCTTTGGA ACAGCAATTA GAAGAAGCCC AAACAGAAAA TTTTAATATT AAGCAAATGA 
     1501 AAGACTTATT TGAACAGAAA GCAGCCCAAC TTGCTACTGA AATTGCAGAT ATAAAGTCAA 
     1561 AGTATGATGA AGAAAGGAGT CTTCGAGAAG CTGCTGAACA AAAAGTGACA CGTCTGACAG 
     1621 AAGAATTAAA CAAAGAGGCA ACTGTAATTC AAGATCTGAA GACGGAACTG CTTCAGAGAC 
     1681 CTGGTATAGA AGATGTTGCC GTGCTAAAGA AAGAACTGGT CCAAGTTCAA ACACTAATGG 
     1741 ATAACATGAC CTTGGAACGT GAGCGAGAAT CTGAAAAACT CAAAGATGAA TGCAAAAAAT 
     1801 TGCAGTCACA ATATGCTAGC TCAGAGGCCA CAATAAGCCA GCTAAGGAGT GAACTTGCCA 
     1861 AAGGCCCCCA GGAAGTTGCT GTATATGTAC AGGAACTACA AAAACTGAAA AGTTCAGTTA 
     1921 ATGAATTAAC ACAAAAAAAT CAGACCTTGA CAGAAAACTT GCTGAAAAAA GAACAAGACT 
     1981 ATACTAAGTT AGAGGAGAAA CATAATGAAG AATCTGTGAG TAAAAAGAAT ATTCAGGCAA 
     2041 CCCTTCATCA AAAAGACCTA GATTGTCAAC AGCTTCAGTC AAGATTGTCT GCATCTGAAA 
     2101 CCTCACTGCA TAGAATACAT GTAGAACTAA GTGAAAAAGG AGAAGCTACT CAAAAGCTCA 
     2161 AAGAAGAATT ATCTGAGGTA GAGACCAAGT ACCAGCATCT AAAGGCGGAG TTTAAGCAGC 
     2221 TACAACAACA GAGAGAAGAA AAGGAGCAGC ATGGGTTACA ACTCCAAAGT GAAATTAATC 
     2281 AATTACATAG CAAACTTCTG GAGACAGAGC GCCAACTAGG GGAAGCTCAT GGTAGGCTGA 
     2341 AGGAACAGAG ACAGCTTTCA AGTGAAAAGT TGATGGATAA AGAACAACAA GTGGCTGATT 
     2401 TACAACTCAA ACTTTCTCGG TTAGAAGAGC AGTTGAAGGA AAAAGTTACA AATTCTACAG 
     2461 AATTGCAGCA TCAATTAGAT AAAACAAAGC AACAGCATCA AGAACAACAG GCTCTTCAGC 
     2521 AAAGCACCAC GGCAAAACTT CGAGAAGCTC AGAATGATTT GGAACAAGTT CTACGTCAAA 
     2581 TTGGCGATAA GGACCAAAAG ATCCAGAACC TTGAAGCTTT ATTACAGAAG AGTAAAGAAA 
     2641 ATATTTCATT ACTAGAAAAA GAAAGAGAAG ATCTTTATGC AAAAATTCAG GCTGGTGAAG 
     2701 GAGAGACTGC TGTTCTTAAC CAGTTACAAG AAAAAAACCA TACACTACAG GAGCAAGTAA 
     2761 CTCAACTAAC AGAGAAGCTG AAGAATCAGT CAGAAAGTCA TAAACAAGCC CAGGAGAATT 
     2821 TGCATGACCA GGTACAAGAG CAGAAGGCAC ATCTTAGAGC TGCACAAGAC CGTGTCCTTT 
     2881 CCCTAGAAAC TAGTGTCAAT GAATTAAATA GTCAATTAAA TGAAAGCAAG GAGAAGGTCT 
     2941 CCCAGCTTGA CATACAGATT AAAGCCAAAA CCGAACTATT ACTATCAGCA GAAGCAGCAA 
     3001 AAACTGCTCA AAGAGCTGAT CTTCAGAATC ATTTGGACAC AGCTCAAAAT GCATTACAAG 
     3061 ATAAACAGCA GGAGTTAAAT AAGATTACTA CTCAGTTGGA TCAGGTCACT GCAAAGTTAC 
     3121 AAGACAAGCA AGAACATTGC AGTCAGCTGG AAAGTCATCT TAAAGAATAT AAAGAGAAAT 
     3181 ACCTCTCTTT AGAACAGAAA ACCGAAGAGC TAGAAGGTCA AATTAAGAAA CTAGAAGCTG 
     3241 ATAGTCTTGA AGTTAAAGCA AGCAAGGAGC AGGCTTTGCA AGATCTACAA CAGCAAAGAC 
     3301 AGCTGAACAC AGATTTAGAG CTCAGAGCCA CAGAATTGAG TAAACAACTT GAAATGGAGA 
     3361 AGGAAATAGT ATCCAGTACA AGATTGGATC TACAGAAAAA ATCTGAAGCC CTTGAAAGTA 
     3421 TCAAGCAAAA GCTTACCAAG CAAGAGGAAG AAAAACAAAT CCTGAAACAA GATTTTGAAA 
     3481 CTTTAAGTCA AGAAACAAAG ATTCAGCATG AGGAATTGAA TAACAGAATT CAAACAACAG 
     3541 TAACAGAACT ACAAAAAGTG AAAATGGAGA AAGAAGCTTT AATGACAGAG CTTTCTACAG 
     3601 TAAAGGACAA ACTATCAAAA GTTTCTGATT CTTTGAAAAA CTCTAAAAGT GAATTTGAAA 
     3661 AGGAGAATCA GAAAGGAAAA GCCGCTATAT TAGACTTGGA AAAAACTTGC AAAGAATTAA 
     3721 AGCATCAACT TCAAGTGCAG ATGGAAAACA CACTTAAGGA ACAGAAGGAA CTGAAAAAGT 
     3781 CACTTGAAAA AGAGAAGGAG GCTTCTCATC AGTTGAAATT GGAACTCAAT TCAATGCAGG 
     3841 AACAACTTAT ACAGGCCCAG AATACTTTAA AACAAAATGA AAAAGAAGAG CAACAACTTC 
     3901 AGGGGAACAT AAATGAGCTA AAGCAATCAA GTGAACAGAA GAAAAAACAA ATTGAAGCAC 
     3961 TCCAAGGAGA GCTTAAAATT GCTGTTTTAC AGAAGACAGA GCTTGAGAAT AAACTACAGC 
     4021 AGCAGTTAAC ACAGGCAGCC CAGGAACTTG CAGCAGAGAA AGAGAAAATA TCAGTATTAC 
     4081 AAAACAACTA TGAAAAAAGT CAGGAAACTT TCAAACAGCT TCAATCTGAT TTCTATGGGA 
     4141 GGGAATCTGA ACTTCTAGCC ACCAGGCAAG ATCTTAAGTC TGTAGAAGAG AAGCTTTCTC 
     4201 TAGCACAGGA GGACTTGATT TCAAACAGAA ATCAAATTGG AAATCAAAAT AAATTGATTC 
     4261 AAGAACTGAA GACTGCCAAG GCTACATTGG AGCAGGATTC AGCAAAGAAA GAACAGCAAT 
     4321 TGCAGGAGCG ATGTAAAGCA CTACAAGACA TTCAGAAAGA AAAGTCACTG AAAGAAAAAG 
     4381 AACTGGTAAA TGAGAAGTCT AAATTGGCAG AGATAGAAGA AATTAAATGT AGACAAGAAA 
     4441 AAGAAATCAC TAAACTAAAC GAAGAACTCA AGTCCCACAA ACTAGAAAGC ATAAAGGAGA 
     4501 TAACAAATCT TAAAGATGCT AAACAGCTTC TAATTCAGCA GAAATTAGAA CTTCAAGGAA 
     4561 AAGCGGACTC CCTGAAGGCA GCTGTTGAAC AGGAGAAGAG AAATCAGCAG ATACTAAAAG 
     4621 ACCAGGTGAA AAAGGAAGAA GAGGAGCTGA AGAAAGAATT TATTGAGAAA GAAGCTAAGT 
     4681 TGCATTCCGA AATAAAAGAA AAGGAAGTAG GAATGAAGAA GCATGAAGAA AATGAGGCTA 
     4741 AACTTACCAT GCAGATTACA GCATTAAATG AAAACTTAGG CACTGTGAAG AAGGAGTGGC 
     4801 AATCTAGTCA ACGGAGAGTT AGTGAGCTTG AGAAACAAAC GGATGACTTA CGGGGTGAAA 
     4861 TTGCAGTATT AGAAGCAACG GTTCAGAATA ATCAAGATGA AAGGAGAGCA CTACTGGAAA 
     4921 GATGTCTTAA AGGAGAAGGT GAAATAGAAA AGCTTCAAAC CAAAGTATTA GAATTGCAAA 
     4981 GAAAGCTGGA TAATACAACT GCAGCAGTGC AGGAGCTGGG CAGAGAAAAC CAATCACTTC 
     5041 AGATCAAACA TACACAAGCG TTGAATAGAA AGTGGGCCGA AGACAATGAA GTACAAAACT 
     5101 GTATGGCCTG TGGGAAAGGC TTTTCAGTAA CAGTGAGACG GCATCACTGC CGACAGTGTG 
     5161 GAAATATCTT CTGTGCTGAA TGTTCAGCCA AAAATGCCTT AACTCCTTCC TCCAAGAAGC 
     5221 CTGTTCGTGT CTGTGATGCA TGTTTCAATG ACTTGCAAGG AACGCGTACG CGGCCGCTCG 
     5281 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     5341 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     5401 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     5461 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     5521 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     5581 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     5641 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     5701 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5761 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     5821 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5881 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5941 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     6001 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     6061 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     6121 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     6181 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     6241 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     6301 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     6361 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     6421 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     6481 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     6541 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     6601 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     6661 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     6721 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6781 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6841 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6901 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6961 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     7021 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     7081 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     7141 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     7201 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     7261 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     7321 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     7381 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     7441 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     7501 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     7561 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     7621 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     7681 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     7741 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7801 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7861 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7921 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7981 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     8041 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     8101 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     8161 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     8221 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     8281 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     8341 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     8401 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     8461 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     8521 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     8581 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     8641 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     8701 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     8761 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     8821 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8881 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8941 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     9001 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     9061 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//