LOCUS       RC215366                8139 bp    DNA     circular  SYN 
DEFINITION  TNK2 (Myc-DDK-tagged)-Human tyrosine kinase, non-receptor, 2 (TNK2), transcript variant 2
ACCESSION   NM_001010938
VERSION     NM_001010938.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4296..4387 
                     /label="Myc-DDK"  
     misc_feature    4417..5002 
                     /label="PolyA signal"  
     misc_feature    4474..4493 
                     /label="Restriction Enzyme"  
     misc_feature    5278..5950 
                     /label="ColE1"  
     misc_feature    6380..7174 
                     /label="Kan/Neo"  
     misc_feature    7209..7511 
                     /label="SV40 ori"  
     misc_feature    7597..8074 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4278..4284 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4278 
                     /label="TNK2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCTCGAGGCC CGGCCCCCCA GGACGCAGGG CAGTGACGCT GCCGGTGCCG 
     1081 CTGCGGGGCG GGGGCTGCGG GCGCTGCTTC TCTCCCTGAC CGCAGCCGCT GGGATTTGGG 
     1141 GCTCCATGGG GGAGAGATCT GCTTACCAGC GCCTGGCTGG GGGCGAGGAG GGACCGCAGA 
     1201 GGCTGGGAGG CGGCAGAATG CAGCCAGAGG AGGGCACAGG CTGGCTGCTG GAGCTGCTGT 
     1261 CCGAGGTGCA GCTGCAACAG TACTTCCTGC GGCTCCGAGA TGACCTCAAC GTCACCCGCC 
     1321 TGTCCCACTT TGAGTACGTC AAGAATGAGG ACCTGGAGAA GATCGGCATG GGTCGGCCTG 
     1381 GCCAGCGGCG GCTGTGGGAG GCTGTGAAGA GGAGGAAGGC CTTGTGCAAA CGCAAGTCGT 
     1441 GGATGAGTAA GGTGTTCAGT GGAAAGCGAC TGGAGGCTGA GTTCCCACCT CATCACTCTC 
     1501 AGAGCACCTT CCGGAAGACC TCGCCCGCCC CTGGGGGCCC AGCAGGGGAG GGGCCCCTGC 
     1561 AGAGCCTCAC CTGCCTCATT GGGGAGAAGG ACCTGCGCCT CCTGGAGAAG CTGGGTGATG 
     1621 GTTCCTTTGG CGTGGTGCGC AGGGGCGAGT GGGACGCGCC CTCAGGGAAG ACGGTGAGTG 
     1681 TGGCTGTGAA GTGCCTGAAG CCCGATGTCC TGAGCCAGCC AGAAGCCATG GACGACTTCA 
     1741 TCCGGGAGGT CAATGCCATG CACTCGCTCG ACCACCGAAA CCTCATCCGC CTCTACGGGG 
     1801 TGGTGCTCAC GCCGCCCATG AAGATGGTGA CAGAGCTGGC ACCTCTGGGA TCGTTGTTGG 
     1861 ACCGGCTACG TAAGCACCAG GGCCACTTCC TCCTGGGGAC TCTGAGCCGC TACGCTGTGC 
     1921 AGGTGGCTGA GGGCATGGGC TACCTGGAGT CCAAGCGCTT TATTCACCGT GACCTGGCTG 
     1981 CCCGCAATCT GCTGTTGGCT ACCCGCGACC TGGTCAAGAT CGGGGACTTT GGGCTGATGC 
     2041 GAGCACTACC TCAGAATGAC GACCATTACG TCATGCAGGA ACATCGCAAG GTGCCCTTCG 
     2101 CCTGGTGTGC CCCCGAGAGC CTGAAGACAC GCACCTTCTC CCATGCCAGC GACACCTGGA 
     2161 TGTTCGGGGT GACACTGTGG GAAATGTTCA CCTACGGCCA GGAGCCCTGG ATCGGCCTCA 
     2221 ACGGCAGTCA GATCCTGCAT AAGATCGACA AGGAGGGGGA GCGGCTGCCC CGGCCCGAGG 
     2281 ACTGTCCCCA GGACATCTAC AACGTCATGG TCCAGTGCTG GGCTCACAAG CCAGAGGACA 
     2341 GACCCACGTT TGTGGCCCTG CGGGACTTCC TGCTGGAGGC CCAGCCCACA GACATGCGGG 
     2401 CCCTTCAGGA CTTTGAGGAA CCGGACAAGC TGCACATCCA GATGAATGAT GTCATCACCG 
     2461 TCATCGAGGG AAGGGCCGAG AACTACTGGT GGCGTGGCCA GAACACACGG ACGCTGTGTG 
     2521 TGGGGCCCTT CCCTCGCAAC GTGGTGACCT CCGTGGCCGG CCTGTCGGCC CAGGACATCA 
     2581 GCCAGCCCCT GCAGAACAGC TTCATCCACA CAGGGCATGG CGACAGTGAC CCCCGCCACT 
     2641 GCTGGGGCTT CCCGGACAGG ATTGACGAAC TGTATCTGGG AAACCCCATG GACCCCCCCG 
     2701 ACCTCCTGAG CGTGGAACTG AGCACCTCCC GGCCCCCCCA GCATCTAGGA GGGGTGAAAA 
     2761 GGGAGCCTCC ACCTCGCCCA CCTCAGCCTG CCTTCTTCAC TCAGAAACCA ACCTATGACC 
     2821 CTGTGAGCGA GGACCAAGAC CCCTTGTCCA GCGACTTCAA GAGGCTGGGC CTGCGGAAGC 
     2881 CAGGCCTGCC CCGAGGGCTG TGGCTGGCGA AGCCCTCGGC GCGGGTGCCG GGCACCAAGG 
     2941 CCAGCCGAGG CAGCGGGGCT GAGGTCACGC TCATCGACTT CGGTGAGGAG CCCGTGGTCC 
     3001 CGGCCCTACG GCCCTGCGCG CCCTCCCTGG CGCAGCTGGC CATGGACGCC TGCTCCCTGC 
     3061 TGGACGAGAC CCCGCCTCAG AGCCCCACGC GGGCACTGCC CCGGCCCCTG CACCCCACGC 
     3121 CTGTGGTGGA CTGGGACGCA CGCCCGCTGC CCCCCCCGCC CGCCTATGAC GACGTGGCCC 
     3181 AGGATGAGGA TGACTTTGAG ATCTGCTCCA TCAACAGCAC CCTCGTGGGC GCGGGGGTCC 
     3241 CTGCCGGGCC CAGCCAGGGC CAGACCAACT ACGCCTTTGT GCCTGAGCAG GCGCGGCCGC 
     3301 CCCCTCCCCT GGAGGACAAC CTGTTCCTCC CGCCCCAGGG TGGGGGCAAG CCGCCCAGCT 
     3361 CCGCACAGAC CGCAGAGATC TTCCAGGCGC TACAGCAGGA GTGCATGAGG CAACTGCAGG 
     3421 CTCCGGCCGG CTCCCCGGCC CCCTCTCCCA GCCCGGGGGG TGACGACAAG CCCCAGGTGC 
     3481 CTCCTCGGGT ACCCATCCCC CCTCGGCCCA CGCGCCCACA CGTCCAGCTG TCTCCAGCCC 
     3541 CCCCGGGCGA GGAGGAGACC AGCCAGTGGC CTGGACCTGC TTCCCCTCCC CGGGTGCCTC 
     3601 CGCGGGAGCC CCTGTCCCCT CAAGGCTCGA GGACACCCAG CCCCCTGGTA CCACCTGGCA 
     3661 GCTCCCCGCT GCCACCCCGG CTCTCAAGCT CACCTGGGAA GACCATGCCC ACCACCCAGA 
     3721 GCTTTGCCTC AGACCCCAAG TACGCCACCC CCCAGGTGAT CCAGGCCCCT GGCCCGCGGG 
     3781 CTGGTCCCTG CATCCTGCCC ATCGTCCGGG ATGGCAAGAA GGTCAGCAGC ACCCACTATT 
     3841 ACTTGCTGCC CGAGCGACCA TCCTACCTGG AGCGCTACCA GCGCTTCCTG CGTGAGGCCC 
     3901 AGAGCCCCGA GGAGCCTACC CCCCTGCCTG TGCCTCTGCT GCTGCCCCCA CCCAGCACCC 
     3961 CAGCCCCCGC CGCCCCCACG GCCACCGTGC GGCCGATGCC CCAGGCTGCC TTGGACCCCA 
     4021 AGGCCAACTT CTCCACCAAC AACAGCAACC CAGGGGCCCG GCCACCACCC CCGAGGGCCA 
     4081 CTGCTCGGCT GCCACAGAGG GGCTGCCCTG GCGATGGGCC AGAGGCGGGC CGGCCAGCAG 
     4141 ACAAGATCCA GATGGTGGAG CAGCTCTTCG GGCTGGGTCT GCGGCCCAGA GGGGAGTGCC 
     4201 ACAAAGTGCT GGAGATGTTC GACTGGAACC TGGAGCAGGC CGGCTGCCAC CTTCTGGGCT 
     4261 CCTGGGGCCC TGCCCACCAC AAGCGCACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     4321 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     4381 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4441 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4501 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4561 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4621 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     4681 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     4741 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     4801 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     4861 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     4921 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     4981 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     5041 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     5101 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     5161 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     5221 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     5281 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     5341 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     5401 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     5461 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     5521 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     5581 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     5641 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     5701 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     5761 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     5821 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     5881 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     5941 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     6001 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     6061 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     6121 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     6181 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     6241 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     6301 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     6361 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     6421 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     6481 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     6541 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     6601 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     6661 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     6721 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     6781 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     6841 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     6901 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     6961 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     7021 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     7081 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     7141 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     7201 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     7261 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     7321 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     7381 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7441 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7501 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     7561 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     7621 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     7681 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     7741 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     7801 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     7861 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     7921 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     7981 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     8041 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     8101 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//