LOCUS       RC215364                8256 bp    DNA     circular  SYN 
DEFINITION  COL28A1 (Myc-DDK-tagged)-Human collagen, type XXVIII, alpha 1 (COL28A1)
ACCESSION   NM_001037763
VERSION     NM_001037763.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4413..4504 
                     /label="Myc-DDK"  
     misc_feature    4534..5119 
                     /label="PolyA signal"  
     misc_feature    4591..4610 
                     /label="Restriction Enzyme"  
     misc_feature    5395..6067 
                     /label="ColE1"  
     misc_feature    6497..7291 
                     /label="Kan/Neo"  
     misc_feature    7326..7628 
                     /label="SV40 ori"  
     misc_feature    7714..8191 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4395..4401 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4395 
                     /label="COL28A1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTGGAACAGA TATTTTGTCT TCTATCTCCT GCTTTTGTCA GCGTTTACGA 
     1081 GTCAAACAGT ATCCGGACAA AGAAAGAAAG GACCAAAATC AAATTTGCTT GCAAGGAAAA 
     1141 GTGATGTCCA GGGCTCCATT TGTTTCATAG ATATTGTCTT CATCGTGGAC AGCTCTGAAA 
     1201 GTTCTAAAAT TGCCCTCTTT GATAAACAGA AAGATTTTGT GGATAGCTTG AGTGACAAGA 
     1261 TTTTCCAATT GACTCCTGGT CGCTCCTTGG AATATGACAT CAAACTGGCA GCCCTTCAGT 
     1321 TTAGCAGCTC TGTCCAAATT GATCCACCTT TTTCTTCCTG GAAGGACCTG CAGACATTTA 
     1381 AGCAGAAGGT CAAGTCTATG AATTTAATAG GGCAAGGTAC CTTCTCTTAT TATGCCATTT 
     1441 CCAATGCCAC TAGGCTACTT AAGAGAGAAG GGCGTAAGGA TGGTGTGAAA GTGGTTTTGC 
     1501 TGATGACTGA TGGCATCGAC CATCCAAAGA ATCCAGATGT TCAAAGTATT TCTGAAGATG 
     1561 CCAGAATTTC AGGAATATCA TTTATCACCA TTGCACTTTC TACGGTAGTC AATGAAGCCA 
     1621 AACTTCGTTT GATTTCTGGG GATTCATCCA GTGAACCCAC TTTACTGTTG AGTGATCCAA 
     1681 CCCTTGTAGA TAAAATTCAA GATCGTCTGG ATATCTTATT TGAAAAGAAG TGTGAACGCA 
     1741 AGATTTGTGA ATGTGAGAAG GGAGATCCAG GTGATCCAGG GCCTCCTGGT ACACATGGAA 
     1801 ATCCAGGTAT CAAAGGTGAG CGAGGACCAA AAGGAAACCC GGGCAACGCT CAAAAAGGAG 
     1861 AAGCTGGAGA AAGAGGTCCA GGGGGAATTC CTGGGTACAA GGGTGACAAG GGTGAACGTG 
     1921 GGGAATGTGG TAAACCAGGG ATAAAAGGTG ACAAGGGATC CCCAGGGCCA TATGGACCAA 
     1981 AGGGACCCAG AGGAATTCAG GGAATTACTG GACCTCCAGG AGACCCAGGC CCAAAGGGGT 
     2041 TTCAAGGCAA TAAGGGTGAG CCAGGTCCTC CTGGTCCTTA TGGTTCTCCA GGAGCTCCTG 
     2101 GAATTGGACA GCAAGGTATT AAGGGAGAAA GAGGCCAAGA AGGAAGACCG GGAGCTCCAG 
     2161 GACCCATTGG AGTTGGTGAG CCTGGACAGC CAGGTCCCCG TGGTCCTGAG GGAGTACCAG 
     2221 GAGAGAGGGG CTTACCCGGA GAAGGATTTC CAGGACCAAA GGGTGAAAAA GGTTCTGAAG 
     2281 GACCAACTGG CCCACAGGGA TTACAAGGCC TGTCAATCAA AGGGGAGAAG GGGGATATAG 
     2341 GACCTGTGGG ACCCCAAGGA CCAATGGGTA TCCCTGGAAT CGGGAGTCAA GGGGAACAGG 
     2401 GAATCCAAGG TCCTATTGGT CCACCTGGTC CACAAGGGCC CGCAGGACAG GGCTTACCTG 
     2461 GTTCCAAGGG AGAAGTAGGC CAAATGGGAC CTACAGGCCC TCGAGGACCA GTGGGAATTG 
     2521 GAGTACAAGG TCCAAAGGGA GAGCCAGGCT CAATAGGGCT CCCAGGACAA CCAGGAGTAC 
     2581 CAGGAGAAGA TGGAGCTGCA GGGAAGAAGG GAGAAGCGGG GCTTCCGGGA GCAAGAGGCC 
     2641 CAGAAGGACC ACCTGGAAAA GGACAGCCTG GCCCCAAGGG TGACGAAGGC AAGAAAGGGA 
     2701 GCAAAGGAAA TCAAGGACAG AGGGGACTTC CAGGGCCCGA AGGACCAAAG GGTGAACCGG 
     2761 GCATTATGGG CCCTTTTGGA ATGCCTGGAA CATCAATTCC TGGACCACCT GGGCCAAAGG 
     2821 GAGATAGAGG AGGACCTGGG ATACCTGGAT TTAAGGGAGA ACCTGGACTT TCTATTCGAG 
     2881 GACCAAAGGG TGTCCAAGGC CCTCGGGGAC CAGTGGGTGC TCCAGGACTC AAAGGTGATG 
     2941 GCTATCCTGG TGTGCCTGGA CCTCGTGGAT TACCAGGACC CCCTGGGCCG ATGGGTTTAC 
     3001 GTGGAGTGGG AGACACTGGA GCAAAGGGAG AGCCTGGGGT CAGAGGCCCT CCAGGTCCTT 
     3061 CTGGGCCTCG GGGCGTAGGA ACCCAAGGGC CAAAGGGTGA TACTGGGCAG AAAGGCTTGC 
     3121 CTGGCCCTCC TGGCCCCCCT GGCTATGGAT CACAGGGAAT TAAAGGGGAA CAAGGACCAC 
     3181 AAGGCTTCCC AGGCCCAAAG GGCACAATGG GCCATGGCCT CCCAGGCCAG AAGGGAGAGC 
     3241 ACGGAGAACG GGGCGATGTG GGAAAGAAAG GTGATAAAGG AGAAATTGGA GAGCCTGGAT 
     3301 CTCCAGGAAA ACAGGGTTTA CAAGGACCCA AAGGAGACCT AGGACTTACA AAAGAAGAAA 
     3361 TTATCAAACT TATTACAGAA ATATGTGGTT GTGGGCCCAA ATGCAAAGAG ACTCCACTAG 
     3421 AGCTGGTGTT TGTGATCGAC AGCTCAGAAA GCGTGGGGCC AGAGAACTTT CAGATCATTA 
     3481 AAAATTTTGT GAAGACTATG GCTGACCGGG TTGCTCTGGA CCTTGCCACG GCCCGCATAG 
     3541 GCATAATCAA CTATAGCCAT AAGGTGGAGA AGGTGGCTAA TTTGAAGCAG TTCTCCAGCA 
     3601 AGGATGACTT CAAGTTGGCT GTGGACAACA TGCAGTATCT GGGGGAAGGC ACATACACAG 
     3661 CCACTGCTCT GCAAGCAGCC AACGACATGT TTGAAGATGC AAGGCCAGGT GTAAAAAAAG 
     3721 TGGCCTTGGT CATCACTGAT GGACAGACAG ATTCTCGTGA TAAAGAGAAA CTGACAGAGG 
     3781 TGGTGAAGAA TGCCAGTGAC ACCAATGTGG AGATATTTGT GATAGGGGTG GTGAAGAAAA 
     3841 ATGATCCCAA CTTTGAAATA TTCCACAAAG AAATGAATCT AATTGCTACT GACCCAGAGC 
     3901 ATGTTTACCA GTTTGATGAT TTCTTTACCC TGCAAGACAC CCTGAAGCAA AAATTGTTTC 
     3961 AAAAAATTTG TGAGGATTTT GATTCCTATC TCGTTCAAAT TTTTGGTTCA TCGTCACCTC 
     4021 AACCTGGATT TGGGATGTCA GGGGAAGAAC TCAGTGAATC TACTCCAGAG CCTCAAAAAG 
     4081 AAATTTCTGA GTCATTGAGT GTCACCAGAG ACCAGGATGA AGATGATAAG GCTCCAGAGC 
     4141 CAACGTGGGC TGATGATCTG CCTGCCACTA CCTCATCTGA GGCCACCACC ACCCCCAGGC 
     4201 CACTGCTCAG CACCCCTGTG GATGGGGCAG AGGATCCTAG ATGTTTGGAA GCCTTGAAGC 
     4261 CTGGAAACTG TGGTGAATAT GTGGTTCGAT GGTATTATGA CAAACAGGTC AACTCTTGTG 
     4321 CCCGATTTTG GTTCAGTGGC TGTAATGGCT CAGGAAATAG ATTCAACAGT GAAAAGGAAT 
     4381 GTCAAGAAAC CTGCATTCAA GGAACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4441 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4501 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4561 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4621 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4681 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4741 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     4801 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     4861 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     4921 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     4981 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     5041 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5101 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5161 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     5221 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     5281 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     5341 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5401 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5461 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5521 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     5581 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     5641 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     5701 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     5761 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     5821 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     5881 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     5941 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     6001 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     6061 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     6121 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6181 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     6241 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6301 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6361 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6421 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6481 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6541 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     6601 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     6661 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     6721 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     6781 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     6841 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     6901 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     6961 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     7021 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     7081 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     7141 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7201 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     7261 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7321 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7381 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7441 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7501 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7561 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7621 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     7681 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     7741 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     7801 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     7861 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     7921 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     7981 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     8041 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     8101 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     8161 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     8221 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//