LOCUS       RC215323                9315 bp    DNA     circular  SYN 
DEFINITION  FYCO1 (Myc-DDK-tagged)-Human FYVE and coiled-coil domain containing 1 (FYCO1)
ACCESSION   NM_024513
VERSION     NM_024513.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5472..5563 
                     /label="Myc-DDK"  
     misc_feature    5593..6178 
                     /label="PolyA signal"  
     misc_feature    5650..5669 
                     /label="Restriction Enzyme"  
     misc_feature    6454..7126 
                     /label="ColE1"  
     misc_feature    7556..8350 
                     /label="Kan/Neo"  
     misc_feature    8385..8687 
                     /label="SV40 ori"  
     misc_feature    8773..9250 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5454..5460 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5454 
                     /label="FYCO1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCTCCACC AATGCAGAGA GCCAGCTCCA GAGAATCATC CGAGACTTGC 
     1081 AAGATGCTGT GACAGAACTA AGCAAAGAAT TTCAGGAAGC AGGGGAACCC ATCACGGATG 
     1141 ACAGCACCAG CTTGCATAAA TTTTCTTATA AACTTGAGTA TCTCCTGCAA TTTGATCAGA 
     1201 AAGAGAAGGC CACCCTCCTG GGCAACAAGA AGGACTACTG GGATTACTTC TGTGCCTGCC 
     1261 TGGCCAAGGT GAAAGGAGCC AATGATGGGA TCCGCTTTGT CAAGTCTATC TCAGAGCTCC 
     1321 GAACATCCTT GGGGAAAGGA AGAGCATTTA TTCGCTACTC CTTGGTGCAC CAGAGGTTGG 
     1381 CAGACACCTT ACAGCAGTGC TTCATGAACA CCAAAGTGAC CAGTGACTGG TACTATGCAA 
     1441 GAAGCCCCTT TCTGCAGCCA AAGCTGAGCT CGGACATTGT GGGCCAACTC TATGAGCTGA 
     1501 CTGAGGTTCA GTTTGACCTG GCGTCGAGGG GCTTTGACTT GGATGCTGCC TGGCCAACAT 
     1561 TTGCCAGGAG GACGCTGACC ACTGGCTCTT CTGCTTACCT GTGGAAACCC CCTAGCCGCA 
     1621 GCTCCAGCAT GAGCAGCTTG GTGAGCAGCT ACCTGCAGAC TCAAGAGATG GTGTCCAACT 
     1681 TTGACCTGAA CAGCCCCCTA AACAACGAGG CATTGGAGGG CTTTGATGAG ATGCGACTAG 
     1741 AGCTGGACCA GTTGGAGGTG CGGGAGAAGC AGCTACGGGA GCGCATGCAG CAGCTGGACA 
     1801 GAGAGAACCA GGAGCTGAGG GCAGCTGTCA GCCAGCAAGG GGAGCAACTG CAGACAGAGA 
     1861 GGGAGAGGGG GCGCACTGCA GCGGAGGACA ACGTTCGCCT CACTTGCTTG GTAGCTGAGC 
     1921 TCCAGAAGCA GTGGGAGGTC ACCCAGGCCA CCCAGAACAC TGTGAAGGAG CTGCAGACAT 
     1981 GCCTGCAGGG CCTGGAGCTA GGAGCAGCAG AGAAGGAGGA GGACTACCAC ACAGCCCTGC 
     2041 GGCGGCTGGA GTCCATGCTG CAGCCCTTGG CACAGGAGCT TGAGGCCACA CGGGACTCAC 
     2101 TGGACAAGAA AAACCAGCAT TTAGCCAGCT TCCCAGGCTG GCTAGCCATG GCTCAGCAGA 
     2161 AGGCAGATAC GGCATCAGAC ACAAAGGGCC GGCAAGAACC TATTCCCAGT GATGCGGCCC 
     2221 AGGAGATGCA GGAGCTAGGG GAGAAGCTTC AAGCCCTAGA AAGGGAGAGA ACCAAGGTCG 
     2281 AGGAGGTCAA CAGACAGCAG AGTGCCCAAC TGGAACAGCT GGTCAAGGAG CTTCAGCTGA 
     2341 AAGAGGATGC CCGGGCCAGC CTGGAGCGCC TGGTGAAGGA GATGGCCCCA CTCCAGGAGG 
     2401 AGTTGTCTGG GAAGGGACAG GAGGCAGACC AGCTCTGGCG ACGGCTGCAG GAGTTGCTGG 
     2461 CCCACACGAG CTCCTGGGAG GAGGAGCTAG CAGAGTTGAG GCGGGAGAAA AAACAGCAAC 
     2521 AGGAGGAGAA GGAGCTGCTG GAGCAGGAGG TCAGGTCTCT GACCCGGCAG CTGCAGTTCC 
     2581 TGGAGACCCA GCTGGCACAG GTGAGCCAAC ATGTGAGTGA CCTGGAGGAG CAGAAGAAGC 
     2641 AGCTCATTCA GGACAAAGAC CACCTCAGCC AGCAGGTGGG TATGCTCGAG CGGCTTGCTG 
     2701 GGCCGCCTGG CCCAGAACTG CCAGTGGCAG GTGAGAAGAA TGAGGCCCTG GTCCCTGTGA 
     2761 ACTCCAGTCT GCAAGAGGCC TGGGGGAAGC CAGAGGAGGA GCAGAGGGGC CTGCAGGAGG 
     2821 CACAGTTAGA CGATACCAAG GTGCAAGAGG GCAGCCAGGA GGAAGAGCTC CGGCAGGCCA 
     2881 ACAGGGAGCT GGAGAAGGAG CTACAGAATG TGGTCGGGCG TAACCAGCTC CTGGAGGGCA 
     2941 AGCTGCAAGC CCTGCAGGCC GATTACCAGG CTTTGCAGCA GCGGGAATCA GCCATCCAGG 
     3001 GCTCCTTGGC CTCCCTGGAG GCCGAGCAGG CCAGCATCCG GCACTTGGGT GACCAGATGG 
     3061 AGGCGAGCTT GCTGGCTGTA AGGAAGGCCA AGGAGGCCAT GAAAGCCCAG ATGGCAGAGA 
     3121 AGGAGGCCAT TCTACAGAGC AAGGAGGGCG AGTGTCAGCA GCTGCGGGAG GAGGTGGAGC 
     3181 AGTGCCAGCA ACTGGCAGAA GCCCGGCACA GAGAGCTTAG GGCTCTCGAG AGCCAGTGCC 
     3241 AGCAGCAGAC CCAGCTGATT GAGGTCCTCA CAGCAGAGAA AGGCCAACAG GGAGTTGGCC 
     3301 CACCCACTGA CAATGAAGCC CGTGAGCTGG CTGCCCAGCT AGCCCTGTCT CAGGCGCAGC 
     3361 TGGAAGTCCA TCAGGGGGAG GTCCAACGGC TGCAGGCTCA GGTGGTGGAC CTCCAGGCCA 
     3421 AGATGCGGGC AGCCCTGGAT GACCAGGACA AGGTGCAGAG CCAGCTAAGC ATGGCTGAGG 
     3481 CCGTCCTGAG GGAGCACAAA ACCCTTGTGC AGCAGCTGAA GGAGCAGAAT GAAGCCCTTA 
     3541 ACAGAGCCCA TGTCCAGGAG CTGCTGCAAT GCTCGGAGCG TGAAGGGGCA CTGCAGGAGG 
     3601 AGAGGGCCGA TGAGGCCCAG CAGAGGGAGG AGGAGCTGCG GGCCCTGCAG GAGGAGCTGT 
     3661 CCCAGGCCAA ATGCAGCTCC GAGGAAGCAC AGCTGGAGCA CGCTGAGCTG CAAGAGCAGC 
     3721 TGCACCGGGC CAACACAGAC ACAGCTGAGC TGGGCATCCA GGTTTGCGCA CTGACCGTGG 
     3781 AAAAGGAGCG AGTGGAGGAG GCACTGGCCT GTGCTGTCCA GGAGCTCCAG GACGCCAAAG 
     3841 AGGCAGCCTC AAGGGAGCGA GAGGGCCTGG AGCGCCAAGT AGCTGGGCTG CAGCAAGAGA 
     3901 AGGAGAGCTT GCAGGAGAAG CTGAAGGCGG CCAAGGCAGC AGCCGGCTCA CTGCCTGGCC 
     3961 TGCAGGCCCA GCTCGCCCAG GCAGAGCAGC GGGCCCAGAG CCTCCAAGAG GCTGCACACC 
     4021 AGGAGCTCAA CACCCTCAAG TTCCAGCTGA GTGCTGAAAT CATGGACTAC CAGAGCAGAC 
     4081 TTAAGAATGC TGGTGAAGAG TGCAAGAGCC TCAGGGGCCA GCTTGAGGAG CAAGGCCGGC 
     4141 AGCTGCAGGC TGCTGAGGAA GCTGTGGAGA AGCTGAAGGC CACCCAAGCA GACATGGGAG 
     4201 AGAAGCTGAG CTGCACTAGC AACCATCTTG CAGAGTGCCA GGCGGCCATG CTGAGGAAGG 
     4261 ACAAGGAGGG GGCTGCCCTG CGTGAAGACC TAGAAAGGAC CCAGAAGGAA CTCGAAAAAG 
     4321 CCACAACAAA AATCCAAGAG TATTACAACA AACTCTGCCA GGAGGTGACA AATCGTGAGA 
     4381 GGAATGACCA GAAGATGCTT GCTGACCTGG ATGACCTCAA CAGAACCAAG AAGTATCTCG 
     4441 AGGAGCGGCT GATAGAGCTG CTCAGGGACA AGGATGCTCT CTGGCAGAAG TCAGATGCCC 
     4501 TGGAATTCCA GCAGAAGCTC AGTGCTGAGG AGAGATGGCT CGGAGACACA GAGGCAAACC 
     4561 ACTGCCTCGA CTGTAAGCGG GAGTTCAGCT GGATGGTGCG GCGGCACCAC TGCAGGATAT 
     4621 GTGGCCGCAT CTTCTGTTAC TACTGCTGCA ACAACTACGT CCTGAGCAAG CACGGTGGCA 
     4681 AAAAGGAGCG CTGCTGCCGA GCCTGTTTCC AGAAGCTCAG TGAAGGCCCT GGCTCCCCTG 
     4741 ATAGCAGTGG CTCAGGCACT AGCCAGGGAG AGCCCAGCCC TGCACTGTCA CCAGCCTCAC 
     4801 CTGGGCCCCA GGCCACAGGA GGCCAAGGAG CAAATACAGA CTACAGGCCA CCGGACGACG 
     4861 CTGTGTTTGA TATCATCACA GATGAGGAAT TGTGCCAGAT ACAGGAGTCC GGCTCCTCTT 
     4921 TGCCTGAAAC ACCCACTGAA ACTGATTCTC TCGACCCAAA TGCGGCTGAA CAGGATACTA 
     4981 CATCAACCTC GCTAACGCCT GAGGACACTG AAGACATGCC CGTGGGGCAG GATTCGGAAA 
     5041 TCTGCCTGCT GAAGTCTGGA GAACTGATGA TCAAAGTACC CCTCACAGTG GATGAGATCG 
     5101 CCAGCTTCGG GGAGGGTAGC AGGGAGCTGT TTGTGAGGTC CAGCACCTAC AGCCTGATCC 
     5161 CCATCACTGT GGCCGAGGCA GGCCTCACCA TCAGCTGGGT CTTCTCCTCT GACCCCAAGA 
     5221 GCATCTCCTT CAGTGTGGTC TTCCAGGAGG CCGAGGACAC ACCGCTGGAT CAGTGTAAGG 
     5281 TCCTCATTCC CACGACCCGA TGCAACTCCC ACAAGGAGAA CATCCAGGGC CAGCTCAAGG 
     5341 TTCGCACACC CGGCATCTAC ATGCTCATCT TCGACAATAC CTTCTCAAGG TTTGTCTCTA 
     5401 AAAAGGTATT TTATCACTTG ACGGTTGATC GGCCTGTGAT CTACGATGGA AGTGATTTCC 
     5461 TGACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     5521 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     5581 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     5641 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     5701 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     5761 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     5821 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     5881 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     5941 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     6001 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     6061 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     6121 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     6181 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     6241 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     6301 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     6361 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     6421 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     6481 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     6541 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     6601 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     6661 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     6721 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     6781 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     6841 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     6901 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     6961 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     7021 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     7081 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     7141 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     7201 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     7261 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     7321 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     7381 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     7441 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     7501 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     7561 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     7621 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     7681 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     7741 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     7801 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     7861 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     7921 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     7981 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     8041 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     8101 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     8161 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     8221 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     8281 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     8341 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     8401 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     8461 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     8521 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     8581 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     8641 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     8701 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     8761 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     8821 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     8881 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     8941 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     9001 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     9061 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     9121 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     9181 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     9241 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     9301 TTTAACGCGA ATTTT
//