LOCUS       RC215286                7434 bp    DNA     circular  SYN 
DEFINITION  LPIN3 (Myc-DDK-tagged)-Human lipin 3 (LPIN3)
ACCESSION   NM_022896
VERSION     NM_022896.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3591..3682 
                     /label="Myc-DDK"  
     misc_feature    3712..4297 
                     /label="PolyA signal"  
     misc_feature    3769..3788 
                     /label="Restriction Enzyme"  
     misc_feature    4573..5245 
                     /label="ColE1"  
     misc_feature    5675..6469 
                     /label="Kan/Neo"  
     misc_feature    6504..6806 
                     /label="SV40 ori"  
     misc_feature    6892..7369 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3573..3579 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3573 
                     /label="LPIN3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAACTACGTG GGGCAGCTGG CGGAGACGGT GTTTGGGACG GTGAAGGAGC 
     1081 TGTACCGGGG CCTGAACCCA GCCACACTGA GCGGCGGCAT TGACGTGCTG GTGGTGAAGC 
     1141 AGGTGGACGG CTCGTTCCGG TGCTCACCCT TCCACGTGCG TTTTGGCAAG CTGGGCGTCC 
     1201 TGCGGTCGCG GGAGAAGGTG GTAGACATTG AGCTCAATGG GGAGCCTGTG GACTTGCACA 
     1261 TGAAGCTTGG GGACAGCGGG GAGGCCTTCT TTGTTCAGGA GCTGGAGAGC GATGATGAAC 
     1321 ATGTGCCTCC CGGCCTGTGC ACCTCACCCA TCCCTTGGGG GGGTCTGTCT GGCTTCCCCT 
     1381 CGGACTCCCA GCTGGGCACT GCCAGTGAGC CTGAGGGCCT CGTCATGGCA GGCACGGCCT 
     1441 CCACTGGGCG GAGGAAGAGG CGTCGCAGGA GGAAACCCAA GCAGAAAGAG GATGCAGTGG 
     1501 CAACTGATTC TAGTCCAGAG GAACTGGAGG CAGGCGCTGA GAGTGAGCTA TCCCTGCCGG 
     1561 AAAAGCTGAG GCCAGAGCCC CCAGGTGTCC AGTTGGAAGA GAAGTCTTCA CTGCAGCCCA 
     1621 AAGACATCTA CCCCTACTCG GATGGCGAGT GGCCCCCCCA GGCCAGCCTC TCAGCAGGTG 
     1681 AGCTAACATC CCCTAAGAGC GACTCGGAGC TGGAGGTGCG GACCCCGGAG CCCAGTCCCC 
     1741 TAAGAGCCGA GTCCCACATG CAGTGGGCCT GGGGGAGGCT GCCTAAGGTG GCCAGAGCTG 
     1801 AGCGGCCCGA GTCCTCAGTG GTCCTTGAAG GCAGAGCTGG GGCAACCTCT CCTCCTCGGG 
     1861 GAGGACCCAG CACTCCCTCT ACCTCTGTGG CTGGCGGCGT GGACCCTTTG GGACTCCCAA 
     1921 TCCAGCAAAC AGAGGCTGGT GCCGACCTTC AGCCTGACAC AGAGGATCCC ACTCTAGTGG 
     1981 GTCCCCCTCT CCACACCCCA GAGACAGAGG AAAGCAAGAC TCAGAGCTCT GGGGACATGG 
     2041 GCCTCCCTCC TGCCTCCAAG TCATGGAGCT GGGCCACTCT GGAGGTTCCA GTTCCCACCG 
     2101 GGCAGCCAGA GAGGGTCTCC AGGGGGAAAG GCTCCCCAAA GAGAAGCCAG CACCTGGGCC 
     2161 CCAGTGACAT CTACCTGGAT GACTTGCCCT CCCTGGACTC TGAGAATGCA GCGCTTTACT 
     2221 TCCCCCAAAG TGACTCTGGG CTGGGGGCCA GAAGATGGAG TGAACCCAGC AGTCAGAAGT 
     2281 CCCTGAGGGA CCCCAACCCT GAACATGAAC CTGAACCCAC TCTGGACACA GTGGATACAA 
     2341 TAGCACTGTC CCTCTGTGGT GGACTGGCTG ACAGCCGGGA CATCTCCCTA GAGAAATTCA 
     2401 ACCAGCACAG CGTCTCTTAC CAGGACCTCA CCAAAAACCC CGGACTTTTG GATGACCCAA 
     2461 ACCTAGTGGT GAAAATCAAT GGAAAGCATT ATAACTGGGC TGTGGCTGCC CCCATGATCC 
     2521 TCTCCCTGCA AGCCTTCCAG AAAAACTTGC CCAAGAGCAC CATGGACAAG CTGGAGAGGG 
     2581 AGAAGATGCC CCGGAAGGGT GGGCGATGGT GGTTTTCCTG GCGACGCAGG GACTTCCTGG 
     2641 CCGAGGAGCG CAGTGCCCAG AAGGAGAAGA CTGCAGCCAA GGAGCAGCAG GGGGAGAAGA 
     2701 CAGAAGTCCT GAGCAGTGAT GACGATGCCC CAGACAGCCC TGTGATCCTG GAGATCCCCT 
     2761 CCTTGCCACC CTCCACTCCA CCCTCCACTC CTACCTACAA GAAGTCCCTC CGCCTCTCCT 
     2821 CCGATCAGAT CCGGCGCCTG AACCTGCAAG AAGGTGCCAA TGATGTGGTC TTCAGCGTGA 
     2881 CCACTCAGTA CCAGGGCACC TGCCGCTGCA AGGCCACCAT CTACCTGTGG AAATGGGACG 
     2941 ACAAGGTGGT CATCTCTGAC ATCGACGGCA CCATCACCAA GTCAGATGCT CTGGGCCATA 
     3001 TCCTGCCCCA GCTGGGGAAA GACTGGACAC ACCAGGGCAT CACCAGTCTC TATCACAAAA 
     3061 TCCAACTAAA TGGGTACAAG TTCCTGTACT GCTCGGCGCG GGCCATTGGC ATGGCGGACC 
     3121 TCACCAAGGG GTACCTGCAG TGGGTGAGCG AGGGGGGCTG TAGCCTCCCC AAGGGCCCCA 
     3181 TCCTTCTGTC TCCCAGCAGC CTCTTCTCTG CCCTCCACAG AGAGGTGATC GAGAAGAAAC 
     3241 CAGAGGTGTT CAAGGTCGCC TGCCTGAGTG ACATCCAGCA GCTGTTTCTG CCCCACGGAC 
     3301 AGCCCTTCTA TGCTGCCTTT GGGAATAGGC CCAATGATGT CTTTGCCTAC CGGCAGGTGG 
     3361 GCCTGCCTGA GTCACGCATC TTCACAGTCA ACCCCCGGGG AGAGCTCATC CAGGAGCTCA 
     3421 TAAAGAACCA CAAATCCACG TATGAGCGGC TTGGTGAAGT GGTCGAGCTC CTCTTCCCAC 
     3481 CTGTGGCCCG TGGCCCCAGC ACAGACCTGG CCAACCCTGA ATACAGTAAC TTCTGCTACT 
     3541 GGCGGGAGCC ACTGCCTGCT GTGGACCTTG ATACCCTGGA CACGCGTACG CGGCCGCTCG 
     3601 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     3661 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     3721 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     3781 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     3841 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     3901 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     3961 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4021 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     4081 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     4141 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     4201 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     4261 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     4321 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     4381 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     4441 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     4501 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     4561 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     4621 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     4681 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     4741 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     4801 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     4861 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     4921 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     4981 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     5041 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     5101 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     5161 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     5221 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     5281 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     5341 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     5401 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     5461 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     5521 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     5581 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     5641 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     5701 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     5761 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     5821 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     5881 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     5941 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     6001 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     6061 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     6121 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     6181 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     6241 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     6301 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     6361 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     6421 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     6481 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     6541 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     6601 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     6661 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     6721 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     6781 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     6841 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     6901 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     6961 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     7021 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     7081 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     7141 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     7201 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     7261 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     7321 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     7381 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//