LOCUS       RC215270                8421 bp    DNA     circular  SYN 
DEFINITION  ATP13A2 (Myc-DDK-tagged)-Human ATPase type 13A2 (ATP13A2), transcript variant 1
ACCESSION   NM_022089
VERSION     NM_022089.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4578..4669 
                     /label="Myc-DDK"  
     misc_feature    4699..5284 
                     /label="PolyA signal"  
     misc_feature    4756..4775 
                     /label="Restriction Enzyme"  
     misc_feature    5560..6232 
                     /label="ColE1"  
     misc_feature    6662..7456 
                     /label="Kan/Neo"  
     misc_feature    7491..7793 
                     /label="SV40 ori"  
     misc_feature    7879..8356 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4560..4566 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4560 
                     /label="ATP13A2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGCGCAGAC AGCAGCCCTC TCGTGGGCAG CACGCCCACC GGTTATGGGA 
     1081 CCCTGACGAT AGGGACATCA ATAGATCCCC TCAGCTCCTC AGTTTCATCC GTGAGGCTCA 
     1141 GCGGCTACTG TGGCAGTCCA TGGAGGGTCA TCGGCTATCA CGTCGTGGTC TGGATGATGG 
     1201 CTGGGATCCC TTTGCTGCTC TTCCGTTGGA AGCCCCTGTG GGGGGTGCGG CTGCGGCTCC 
     1261 GGCCCTGCAA CCTGGCCCAC GCCGAAACAC TCGTTATCGA AATAAGAGAC AAAGAGGATA 
     1321 GTTCCTGGCA GCTCTTCACT GTCCAGGTGC AGACTGAGGC CATCGGCGAG GGCAGCCTGG 
     1381 AGCCGTCCCC ACAGTCCCAG GCAGAGGATG GCCGGAGCCA GGCGGCAGTT GGGGCGGTAC 
     1441 CAGAGGGTGC CTGGAAGGAT ACGGCCCAGC TCCACAAGAG CGAGGAGGCG GTGAGTGTCG 
     1501 GACAGAAGCG GGTGCTGCGG TATTACCTCT TCCAGGGCCA GCGCTATATC TGGATCGAGA 
     1561 CCCAGCAAGC CTTCTACCAG GTCAGCCTCC TGGACCATGG CCGCTCTTGT GACGACGTCC 
     1621 ACCGCTCCCG CCATGGCCTC AGCCTCCAGG ACCAAATGGT GAGGAAGGCC ATTTACGGCC 
     1681 CCAACGTGAT CAGCATACCG GTCAAGTCCT ACCCCCAGCT GCTGGTGGAC GAGGCACTGA 
     1741 ACCCCTACTA TGGGTTCCAG GCCTTCAGCA TCGCGCTGTG GCTGGCTGAC CACTACTACT 
     1801 GGTACGCCCT GTGCATCTTC CTCATTTCCT CCATCTCCAT CTGCCTGTCG CTGTACAAGA 
     1861 CCAGAAAGCA AAGCCAGACT CTAAGGGACA TGGTCAAGTT GTCCATGCGG GTGTGCGTGT 
     1921 GCCGGCCAGG GGGAGAGGAA GAGTGGGTGG ACTCCAGTGA GCTAGTGCCC GGAGACTGCC 
     1981 TGGTGCTGCC CCAGGAGGGT GGGCTGATGC CCTGTGATGC CGCCCTGGTG GCCGGCGAGT 
     2041 GCATGGTGAA TGAGAGCTCT CTGACAGGAG AGAGCATTCC AGTGCTGAAG ACGGCACTGC 
     2101 CGGAGGGGCT GGGGCCCTAC TGTGCAGAGA CACACCGGCG GCACACACTC TTCTGCGGGA 
     2161 CCCTCATCTT GCAGGCCCGG GCCTATGTGG GACCGCACGT CCTGGCAGTG GTGACCCGCA 
     2221 CAGGGTTCTG CACGGCAAAA GGGGGCCTGG TGAGCTCCAT CTTGCACCCC CGGCCCATCA 
     2281 ACTTCAAGTT CTATAAACAC AGCATGAAGT TTGTGGCTGC CCTCTCTGTC CTGGCTCTCC 
     2341 TCGGCACCAT CTACAGCATC TTCATCCTCT ACCGAAACCG GGTGCCTCTG AATGAGATTG 
     2401 TAATCCGGGC TCTCGACCTG GTGACCGTGG TGGTGCCACC TGCCCTGCCT GCTGCCATGA 
     2461 CTGTGTGCAC GCTCTACGCC CAGAGCCGAC TGCGGAGACA GGGCATTTTC TGCATCCACC 
     2521 CACTGCGCAT CAACCTGGGG GGCAAGCTGC AGCTGGTGTG TTTCGACAAG ACGGGCACCC 
     2581 TCACTGAGGA CGGCTTAGAC GTGATGGGGG TGGTGCCCCT GAAGGGGCAG GCATTCCTGC 
     2641 CCCTGGTCCC AGAGCCTCGC CGCCTGCCTG TGGGGCCCCT GCTCCGAGCA CTGGCCACCT 
     2701 GCCATGCCCT CAGCCGGCTC CAGGACACCC CCGTGGGCGA CCCCATGGAC TTGAAGATGG 
     2761 TGGAGTCTAC TGGCTGGGTC CTGGAGGAAG AGCCGGCTGC AGACTCAGCA TTTGGGACCC 
     2821 AGGTCTTGGC AGTGATGAGA CCCCCACTTT GGGAGCCCCA GCTGCAGGCA ATGGAGGAGC 
     2881 CCCCGGTGCC AGTCAGCGTC CTCCACCGCT TCCCCTTCTC TTCGGCTCTG CAGCGCATGA 
     2941 GTGTGGTGGT GGCGTGGCCA GGGGCCACTC AGCCCGAGGC CTACGTCAAA GGCTCCCCGG 
     3001 AGCTGGTGGC AGGGCTCTGC AACCCCGAGA CAGTGCCCAC CGACTTCGCC CAGATGCTGC 
     3061 AGAGCTATAC AGCTGCTGGC TACCGTGTCG TGGCCCTGGC CAGCAAGCCA CTGCCCACTG 
     3121 TGCCCAGCCT GGAGGCAGCC CAGCAACTGA CGAGGGACAC TGTGGAAGGA GACCTGAGCC 
     3181 TCCTGGGGCT GCTGGTCATG AGGAACCTAC TGAAGCCGCA GACAACGCCA GTTATCCAGG 
     3241 CTCTGCGAAG GACCCGCATC CGCGCCGTCA TGGTGACAGG GGACAACCTG CAGACAGCGG 
     3301 TGACTGTGGC CCGGGGCTGT GGCATGGTGG CCCCCCAGGA GCATCTGATC ATCGTCCACG 
     3361 CCACCCACCC TGAGCGGGGT CAGCCTGCCT CTCTCGAGTT CCTGCCGATG GAGTCCCCCA 
     3421 CAGCCGTGAA TGGCGTTAAG GATCCTGACC AGGCTGCAAG CTACACCGTG GAGCCAGACC 
     3481 CCCGATCCAG GCACCTGGCC CTCAGCGGGC CCACCTTTGG TATCATTGTG AAGCACTTCC 
     3541 CCAAGCTGCT GCCCAAGGTC CTGGTCCAGG GCACTGTCTT TGCCCGCATG GCCCCTGAGC 
     3601 AGAAGACAGA GCTGGTGTGC GAGCTACAGA AGCTTCAGTA CTGCGTGGGC ATGTGCGGAG 
     3661 ACGGCGCCAA TGACTGTGGG GCCCTGAAGG CGGCTGATGT CGGCATCTCG CTGTCCCAGG 
     3721 CAGAAGCCTC AGTGGTCTCA CCCTTCACCT CGAGCATGGC CAGTATTGAG TGCGTGCCCA 
     3781 TGGTCATCAG GGAGGGGCGC TGTTCCCTTG ACACTTCGTT CAGCGTCTTC AAGTACATGG 
     3841 CTCTGTACAG CCTGACCCAG TTCATCTCCG TCCTGATCCT CTACACGATC AACACCAACC 
     3901 TGGGTGACCT GCAGTTCCTG GCCATCGACC TGGTCATCAC CACCACAGTG GCAGTGCTCA 
     3961 TGAGCCGCAC GGGGCCAGCG CTGGTCCTGG GACGGGTGCG GCCACCGGGG GCGCTGCTCA 
     4021 GCGTGCCCGT GCTCAGCAGC CTGCTGCTGC AGATGGTCCT GGTGACCGGC GTGCAGCTAG 
     4081 GGGGCTACTT CCTGACCCTG GCCCAGCCAT GGTTCGTGCC TCTGAACAGG ACAGTGGCCG 
     4141 CACCAGACAA CCTGCCCAAC TACGAGAACA CCGTGGTCTT CTCTCTGTCC AGCTTCCAGT 
     4201 ACCTCATCCT GGCTGCAGCC GTGTCCAAGG GGGCGCCCTT CCGCCGGCCG CTCTACACCA 
     4261 ATGTGCCCTT CCTGGTGGCC CTGGCGCTCC TGAGCTCCGT CCTGGTGGGC CTTGTCCTGG 
     4321 TCCCCGGCCT CCTGCAGGGG CCGCTGGCGC TGAGGAACAT CACTGACACC GGCTTCAAGC 
     4381 TGCTGCTGCT GGGTCTGGTC ACCCTCAACT TCGTGGGGGC CTTCATGCTG GAGAGCGTGC 
     4441 TAGACCAGTG CCTCCCCGCC TGCCTGCGCC GCCTCCGGCC CAAGCGGGCC TCCAAGAAGC 
     4501 GCTTCAAGCA GCTGGAACGA GAGCTGGCCG AGCAGCCCTG GCCGCCGCTG CCCGCCGGCC 
     4561 CCCTGAGGAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     4621 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     4681 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     4741 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4801 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     4861 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     4921 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     4981 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     5041 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     5101 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     5161 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     5221 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5281 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5341 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     5401 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     5461 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     5521 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     5581 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     5641 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     5701 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     5761 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     5821 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     5881 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     5941 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     6001 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     6061 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     6121 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     6181 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     6241 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     6301 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     6361 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     6421 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     6481 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     6541 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     6601 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     6661 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     6721 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     6781 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     6841 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     6901 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     6961 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     7021 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     7081 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     7141 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     7201 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     7261 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     7321 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     7381 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     7441 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     7501 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     7561 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     7621 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     7681 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7741 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7801 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     7861 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     7921 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     7981 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     8041 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     8101 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     8161 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     8221 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     8281 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     8341 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     8401 CAAAAATTTA ACGCGAATTT T
//